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Introduction Nephrogenic ascites is an uncommon disorder associated with grave prognosis. Studies on etiopathogenesis and outcomes are scarce. This study aimed to identify the etiologies of ascites in patients with chronic kidney disease (CKD) and estimate the proportion of nephrogenic ascites and the 90-day mortality. Methods This was a prospective, observational, and hospital-based study. Consecutive patients with CKD admitted to a tertiary care government teaching hospital were recruited. History, examination, investigations, and evaluation of the etiology of ascites were performed. Ascites was classified into high and low serum albumin-ascites gradient types. Patients with ascites were also followed up for three months to monitor for worsening symptoms, further workup (if necessary), and mortality. Results A total of 355 patients were recruited, with 72.5% being males. Of these, 76 were newly diagnosed with CKD. The most common comorbidities were diabetes mellitus and hypertension. Forty patients had ascites with a mean duration of CKD and hemodialysis of 20.9±23.1 months and 9.3±15.5 months, respectively. Thirteen of the 40 patients with ascites were lost to follow-up. Among the remaining 27, 13 died during follow-up. A known etiology was seen in 29 of the 40 (72.5%) patients. The multiple etiologies group (any combination of cardiac or liver disease, malignancy, and hypothyroidism) constituted 21 patients. Overall, among the 40 patients with ascites, 11 (27.5%) had nephrogenic ascites of whom, four died during follow-up. Conclusions Nephrogenic ascites was observed in 11 patients. Most patients with ascites in CKD have an identifiable etiology. The prognosis of ascites in patients with CKD in our study was dismal.
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Total anomalous pulmonary venous connection (TAPVC) is a rare congenital cardiovascular malformation in which all four pulmonary veins anomalously drain into the right atrium (RA) either directly or indirectly. There are four main types based on the site of connection. Any type of TAPVC may be associated with obstruction and presents early in the neonatal period with cyanosis, tachycardia, or respiratory distress. We present four cases of all types of TAPVC and its imaging findings in multidetector computed tomography (MDCT) angiography. Cardiac CT and magnetic resonance imaging (MRI) are very useful in delineating the anatomy and drainage pathway of anomalous pulmonary veins. MDCT angiography is noninvasive and easily available, and rapid image acquisition is possible with high spatial resolution. Since early diagnosis and surgical correction are necessary for the survival of these neonates, rapid image acquisition using MDCT angiography can be preferred over MRI.
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Background: Congenital diaphragmatic hernia (CDH) is an anomaly with significant morbidity in neonates. It has been traditionally managed by an open approach with a recent trend toward a minimally invasive approach. Aim: This is a retrospective study of our institutional experience with neonatal thoracoscopic management of CDH, with the impact of few technical nuances. Patients and Methods: The data was collected on neonatal thoracoscopic CDH repair between January 2015 and December 2018, in terms of the demographics, intra-operative parameters, post-operative status, recurrence, and mortality. While analyzing data, we found few technical modifications adopted by the surgeon such as trimming the margin of the defect, use of prosthetic mesh overlay reinforcement for repairs under tension, and to prefer extra-corporeal knotting along with higher placement of trocar, temporary increase in CO2, maximal use of muscle relaxant, extra-corporeal corner hitch stitch at some point, and continuation for further cases. An internal comparison was made to analyze the technical modifications influencing the outcomes, by dividing them into two groups, those with (group A) and without modifications (group B). The data was analyzed using SPSS software (IBM, Version 23). A P value of <0.05 was considered statistically significant. Results: Out of 45 newborns 64.4% were males with an average birth weight of 2.6 kg. Baseline variables were comparable between the groups. The operating time significantly reduced after a higher-level camera port was used (P-value: 0.0001). The mean follow-up was 30.8 months. There were totally seven recurrences (6 in group A and 1 in group B), all within 12 months. Seven parents gave the overall post-treatment feedback as "unsatisfied". The operating time, recurrence rate, and parental satisfaction feedback were significantly less in group B (P-value: 0.001). Conclusion: We recommend trimming the margin of the defect, use of prosthetic mesh overlay reinforcement for repairs under tension, and to prefer extra-corporeal knotting along with higher placement of trocar, temporary increase in CO2, maximal use of muscle relaxant, extra-corporeal corner hitch stitch to reduce the operating time, and recurrence after thoracoscopic CDH repair.
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Hernias Diafragmáticas Congénitas , Masculino , Recién Nacido , Humanos , Femenino , Hernias Diafragmáticas Congénitas/cirugía , Estudios Retrospectivos , Toracoscopía , Dióxido de Carbono , Resultado del TratamientoRESUMEN
Bisphenol-A (BPA) is one of the extensively studied estrogenic endocrine disrupting chemicals (EDC) with ubiquitous exposure among humans and wildlife. While there are literature reporting the association of dysregulated Brain-derived neurotrophic factor (BDNF) expression levels with altered cognitive and emotional behaviour such as anxiety-like and stress behaviour in animal models, there are no studies in BPA that investigate these altered neurobehavioural outcomes in parallel with the expression of intracellular proteins involved in BDNF signaling pathway. In this study, pregnant Wistar rats were exposed to BPA through water (25 µg/L, 250 µg/L, and 2.5 mg/L) during gestation day (GD) 9-21. Prenatal BPA exposure, increased anxiety-like behaviour in males and decreased exploratory behaviour in both male and female offspring. Downregulation of both BDNF and CYP19A1 genes were observed in male BPA-exposed offspring, whereas in females, the expression was upregulated. The expression of p-AKT, p-MEK and p-ERK proteins were increased in males, while in females, it decreased. Both the male and the female BPA-exposed offspring exhibited elevated levels of DNMT1 protein. The sex-specific alteration in the expression of CYP19A1 and DNA methyltransferase 1 (DNMT1) suggests that both hormonal and epigenetic dysregulation could underlie the long-term BPA-induced effect on anxiety-like behaviour in the offspring.
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Ansiedad/inducido químicamente , Aromatasa/metabolismo , Conducta Animal/efectos de los fármacos , Compuestos de Bencidrilo/toxicidad , Factor Neurotrófico Derivado del Encéfalo/metabolismo , Disruptores Endocrinos/toxicidad , Fenoles/toxicidad , Efectos Tardíos de la Exposición Prenatal , Factores Sexuales , Animales , Compuestos de Bencidrilo/administración & dosificación , Disruptores Endocrinos/administración & dosificación , Femenino , Masculino , Fenoles/administración & dosificación , Embarazo , Ratas , Transducción de SeñalRESUMEN
BACKGROUND AND AIMS: Doppler renal resistive index (RI) has been studied to find its association with postoperative acute kidney injury (AKI). This study was conducted to evaluate the usefulness of preoperative RI, postoperative RI and RI variation before and after surgery expressed as a percentage (% RI) for early AKI detection in major abdominal surgery. METHODS: This was a single-centre, prospective observational trial performed in the critical care unit of an academic hospital. Eligible patients posted for major abdominal surgery under general anaesthesia using intraperitoneal approach with at least two predefined risk factors for AKI were included in the study. Renal RI was measured preoperatively and on postoperative day zero. Statistical comparisons were performed for various parameters between the AKI and the non-AKI groups. Pre- and postoperative RI receiver operating characteristics (ROC) curves were drawn and areas under the curves computed. Positive and negative predictive values, sensitivity, specificity and positive and negative likelihood ratios were calculated. RESULTS: A total of 69 subjects were enrolled, of which 14 developed AKI in the postoperative period. The mean resistive indices measured were 0.65 ± 0.09 and 0.74 ± 0.09 in the pre- and postoperative periods, respectively. The area under the ROC curve in the postoperative RI was 0.732 with 95% confidence intervals of 0.592-0.871. This most accurate cut-off value to detect postoperative AKI with sensitivity 57.1% and specificity of 85.5% was 0.77. CONCLUSION: Postoperative RI can detect early AKI after major abdominal surgery.
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Parabens are widely used as antimicrobial preservatives. Recent studies have reported the endocrine disrupting effects of these chemicals, especially methylparaben. Previously, we have reported the alteration in Vtg gene expression upon exposure to environmentally relevant doses of methylparaben in zebrafish (Danio rerio) embryos. However, studies reporting neurobehavioural outcomes on exposure to methylparaben are limited. Therefore, this study was aimed at investigating the methylparaben-induced effects on developmental and neurobehavioural endpoints. Zebrafish embryos were exposed to sub-lethal concentrations of methylparaben: 0.1â¯ppb, 1â¯ppb, 10â¯ppb and 100â¯ppb. Alterations in developmental landmarks such as heart rate and hatching percentage were observed in embryos exposed to 10â¯ppb and 100â¯ppb of methylparaben. Results obtained from the novel tank diving test established that anxiety-like behaviour is induced in larvae exposed to 0.1â¯ppb and 1â¯ppb of methylparaben. A significant inhibition in the acetylcholinesterase (AChE) activity was also recorded in methylparaben-exposed groups. An increase in cortisol levels was observed in the exposed groups, which further supports the observations made in the novel tank diving test, establishing methylparaben as an anxiogenic agent even at sub-lethal concentrations. The underlying molecular mechanism needs further elucidation to investigate whether the behavioural effects are proximally or distally induced by early developmental exposure to methylparaben.
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Acetilcolinesterasa/metabolismo , Ansiedad/patología , Conducta Animal , Embrión no Mamífero/enzimología , Embrión no Mamífero/patología , Hidrocortisona/metabolismo , Parabenos/toxicidad , Pez Cebra/embriología , Animales , Conducta Animal/efectos de los fármacos , Buceo , Embrión no Mamífero/efectos de los fármacos , Disruptores Endocrinos/toxicidad , Frecuencia Cardíaca/efectos de los fármacos , Larva/efectos de los fármacos , Larva/metabolismo , Contaminantes Químicos del Agua/toxicidadRESUMEN
A 7-year-old boy presented with a chronic, indurated, tender left thigh swelling in association with a hypertensive emergency. He had a bilateral moderate degree of hydronephrosis and a left perinephric abscess, and MRI features of posterior reversible encephalopathy syndrome. Histopathological examination of the biopsy specimen demonstrated eosinophilic fasciitis with filamentous fungi. Basidiobolus ranarum was isolated from the culture. The fungus was also isolated from a perinephric fluid aspirate. Computerised tomography of the abdomen demonstrated features consistent with fungal invasion of the pelvic floor muscles and urinary bladder, leading to bilateral hydronephrosis. He required multiple antihypertensive drug therapy and was treated with intravenous amphotericin B, oral itraconazole and potassium iodide. Antihypertensive agents were discontinued after 2 weeks of antifungal therapy. At 6-months follow-up, the hydronephrosis had resolved completely. Perinephric abscess associated with basidiobolomycosis has not been reported previously.
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Absceso/diagnóstico , Entomophthorales/aislamiento & purificación , Hidronefrosis/diagnóstico , Hipertensión/diagnóstico , Perinefritis/diagnóstico , Cigomicosis/complicaciones , Cigomicosis/microbiología , Absceso/patología , Anfotericina B/administración & dosificación , Antifúngicos/administración & dosificación , Antihipertensivos/administración & dosificación , Biopsia , Niño , Histocitoquímica , Humanos , Hidronefrosis/patología , Hipertensión/tratamiento farmacológico , Hipertensión/patología , Itraconazol/administración & dosificación , Imagen por Resonancia Magnética , Masculino , Microscopía , Perinefritis/patología , Yoduro de Potasio/administración & dosificación , Radiografía Abdominal , Tomografía Computarizada por Rayos X , Resultado del Tratamiento , Cigomicosis/tratamiento farmacológicoRESUMEN
MethylParaben (MP), a methyl ester of p-hydroxybenzoic acid, is used as an anti-microbial preservative in foods, drugs and cosmetics for decades. It enters the aquatic environment, and can have toxic effects on aquatic organisms. Little is known on the developmental toxicity of MP exposure to zebrafish during early life stages. In this study, the developmental effects of MP were evaluated in embryo-larval zebrafish (at concentrations ranging from 100µM, 200µM, 400µM, 800µM and 1000µM for 96h post fertilization (hpf). The survival, hatching, heart beat rate and developmental abnormalities were observed in the embryos exposed to MP. MP exposure resulted in decreased heart rate and hatching rate. Defects including pericardial edema blood cell accumulation and bent spine were observed in all the treated concentration, except at 100µM. With increasing concentrations, the frequency of these defects increased. The 96 hpf LC50 of MP was calculated to be 428µM (0.065mg/L). Furthermore, RT-PCR result showed that in larval zebrafish exposed to 100µM (0.015mg/L) of MP till 96 hpf, expression of vitellogenin I (Vtg -I) was significantly upregulated compared to the control group. This data suggest that even though lower concentrations of MP do not cause phenotypic malformations, it leads to dysregulated expression of estrogenic biomarker gene Vtg-I.
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Embrión no Mamífero/efectos de los fármacos , Disruptores Endocrinos/toxicidad , Parabenos/toxicidad , Vitelogeninas/biosíntesis , Contaminantes Químicos del Agua/toxicidad , Pez Cebra/embriología , Animales , Relación Dosis-Respuesta a Droga , Embrión no Mamífero/anomalías , Embrión no Mamífero/metabolismo , Larva , Dosificación Letal Mediana , Reproducción/efectos de los fármacos , Regulación hacia Arriba , Vitelogeninas/genética , Pez Cebra/anomalías , Pez Cebra/genéticaRESUMEN
There is a widespread exposure of general population, including pregnant women and developing fetuses, to the endocrine disrupting chemicals (EDCs). These chemicals have been reported to be present in urine, blood serum, breast milk and amniotic fluid. We aimed to investigate the association between the maternal exposure and in utero fetal exposure levels of these chemicals to study their transfer from maternal to fetal unit indicating prenatal exposure. Samples of maternal blood and amniotic fluid were collected as set from 53 pregnant women at full term. Nine phenolic EDCs, methyl paraben (MP; 20.92ng/mL and 18.92ng/mL), ethyl paraben (EP; 1.97ng/ mL and 1.89ng/mL), propyl paraben (PP; 19.22ng/mL and 18.82ng/mL), butyl paraben (BP; 1.11ng/mL and 1.37ng/mL), p-hydroxybenzoic acid (PHBA; 29.99ng/mL and 26.15ng/mL), bisphenol A (BPA; 7.43ng/mL and 7.75ng/mL), triclosan (TCS; 7.17ng/mL and 7.04ng/mL), octyl phenol (OP; 5.46ng/mL and 5.72ng/mL) and nonyl phenol (NP; 9.38ng/mL and 8.44ng/mL), were simultaneously detected in samples of maternal blood plasma and amniotic fluid respectively using Gas Chromatography-Mass Spectrometry (GC-MS). Highest positive correlation was found for total concentration of 4-nonyl phenol, NP (r=0.575, p<0.001), whereas the lowest positive correlation was found for free form of bisphenol A, BPA (r=0.343, p<0.05), when compared between the two matrices. Our results suggest that maternal exposure to several EDCs is positively associated with in utero exposure to the developing fetus. Future studies should focus on collection of amniotic fluid at different trimesters and the corresponding maternal samples to better characterize the pharmacokinetics and the associated disease etiologies of these EDCs during fetal development.
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Líquido Amniótico/química , Análisis Químico de la Sangre , Disruptores Endocrinos/análisis , Exposición Materna/estadística & datos numéricos , Fenoles/análisis , Adulto , Compuestos de Bencidrilo/análisis , Compuestos de Bencidrilo/sangre , Disruptores Endocrinos/sangre , Monitoreo del Ambiente/métodos , Femenino , Cromatografía de Gases y Espectrometría de Masas , Humanos , India/epidemiología , Parabenos/análisis , Fenoles/sangre , Embarazo , Adulto JovenRESUMEN
The effects of cadmium (CdCl2·7H2O) on cellulase, urease, amylase, invertase and phosphatase were assessed for a period of 45 days in the presence and absence of earthworms [Lampito mauritii (Kinberg)] in alfisol soil. The activities of all enzymes significantly increased with longer incubation times (45 days) under laboratory conditions in both control and Cd-amended soils (both with and without earthworm incubation). However, the activities of all enzymes decreased with increasing Cd concentrations under laboratory conditions, both in the presence and absence of earthworms. In the presence of earthworms, cellulase, urease, invertase and amylase activities increased. However, phosphatase activity was lower in most of the Cd-amended soils in the presence of earthworms compared to its activity levels in soils lacking earthworms. These results show that earthworms modulated the stress imposed by Cd by providing suitable substrates, which in turn acted as stimulants for extracellular enzyme secretion by microbes, and by removing Cd through its accumulation in the tissues of the earthworms.
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Cadmio/toxicidad , Oligoquetos/fisiología , Ureasa/análisis , Animales , Biodegradación Ambiental , Monitoreo del Ambiente , Suelo/química , Contaminantes del Suelo/análisis , Ureasa/metabolismoRESUMEN
BACKGROUND: Chronic hypoxemia is generally attributed to primary cardiac or pulmonary entities. CASE CHARACTERISTICS: A 9-year-old boy presenting with cyanosis, clubbing and hypoxemia, without icterus or hepatosplenomegaly. Cardiovascular and respiratory system examinations were normal. OUTCOME: He was diagnosed as type IB Abernethy malformation, a rare cause of hepatopulmonary syndrome. MESSAGE: Pediatricians should consider hepatopulmonary syndrome in the differential diagnosis of chronic hypoxemia, even in the absence of jaundice or hepatosplenomegaly.
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Malformaciones Arteriovenosas , Síndrome Hepatopulmonar , Hipoxia , Niño , Cianosis , Humanos , MasculinoRESUMEN
Scorpion sting is one of the common paediatric toxicological problems encountered in southern India. This rural emergency often results in an autonomic storm causing peripheral circulatory failure and/or congestive cardiac failure, leading to pulmonary oedema. A rare case of scorpion sting envenoming in an 11-year-old boy that led to local cellulitis, dyspnoea and congestive heart failure is presented. This was followed by a persistent high-grade fever which lasted for more than 2 weeks and was complicated by fatal Staphylococcus aureus infective endocarditis and septic pulmonary embolism. Although infective endocarditis has been reported occasionally in adults following scorpion sting, this is the first case of infective endocarditis in a native valve in a child following scorpion sting. The literature is reviewed and the mechanisms for this association are discussed.
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Endocarditis/diagnóstico , Endocarditis/patología , Neumonía/diagnóstico , Neumonía/patología , Embolia Pulmonar/diagnóstico , Embolia Pulmonar/patología , Picaduras de Escorpión/complicaciones , Niño , Endocarditis/complicaciones , Resultado Fatal , Humanos , India , Masculino , Neumonía/complicaciones , Embolia Pulmonar/complicaciones , Infecciones Estafilocócicas/diagnóstico , Infecciones Estafilocócicas/patología , Staphylococcus aureus/aislamiento & purificaciónRESUMEN
Tuberculous infection of the cavernous sinus and Meckel's cave is extremely rare. In this report, we describe a patient with tuberculoma of the cavernous sinus and Meckel's cave, extending to the petrous apex. The patient underwent microsurgical excision of the lesion and antitubercular chemotherapy resulting in a good outcome. We describe the diagnostic difficulties and review the relevant literature.
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Seno Cavernoso/patología , Duramadre/patología , Tuberculoma/diagnóstico , Niño , Femenino , Humanos , Tuberculoma/complicaciones , Tuberculoma/tratamiento farmacológico , Tuberculoma/cirugíaRESUMEN
Six years old boy underwent elective inguinal exploration for left congenital hernia. Per- operatively, an elongated, purplish-red, fleshy band of tissue was found inside the sac, adherent to the upper pole of testis. Biopsy was taken and the wound closed. An MRI done after 4 weeks proved the origin of the band from spleen. Laparotomy and excision of the band was done. The histo-pathology of the specimen was reported as normal splenic tissue. The above features are consistent with a diagnosis of spleno - gonadal fusion (SGF).
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Soils samples collected from five different areas (S1-S5) around electroplating industries in the city of Coimbatore were analysed for the activities of selected enzymes (cellulase, phosphatase, amylase, urease, and invertase) in the presence and absence of the earthworm Lampito mauritii (Kinberg). Heavy metal analysis of soils showed that chromium (<504 mg/kg) and copper (<28.1mg/kg) contents were much higher than cadmium (<10.60 mg/kg) except in S5, where cadmium (10.6 mg/kg) was higher than the copper. Except for phosphatase, the activities of all enzymes increased with increasing period of incubation under laboratory conditions, both with and without earthworms. The results of the three-way ANOVA (effect of three factors- worms-with and without addition, soil and incubation time), however, showed that there was no significant difference between enzyme activities (with and without earthworm) and soil and incubation time for amylase and urease activity. Further, no significant difference was found between soils for cellulase activity and between all the above factors for urease activity. The results concluded that though the earthworms died at the end of the incubation period, the resultant increase or decrease in the enzymatic activity may be attributed to the metabolic activities of the worms during their lifetime in the experimental container. Also, the worms after death may have provided suitable substrate for the growth of the microorganisms thereby influencing enzyme activity.
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Hidrolasas/metabolismo , Metales Pesados/análisis , Oligoquetos , Contaminantes del Suelo/análisis , Animales , Ciudades , Galvanoplastia , Monitoreo del Ambiente , IndiaRESUMEN
AIM: To estimate the arylesterase activity of serum paraoxonase-1 (PON1-ARE), which is reported to have an antioxidant and antiatherogenic potential and to correlate with plasma homocysteine (Hcys) and plasma TBARS in young adult central retinal venous occlusion (CRVO) patients. METHODS: A case-control prospective study carried out in 10 CRVO patients (mean age 27+/-5 years; 7 males, 3 females) and 20 healthy controls (mean age 29+/-5 years; 15 males, 5 females). RESULTS: The CRVO patients showed a significantly lowered serum PON1-ARE activity (P=0.009) along with a significant increase in the levels of plasma Hcys (P=0.018) when compared to the control subjects. There was a negative correlation between serum PON1-ARE and plasma Hcys levels (P=0.058) as well as between PON1-ARE and plasma TBARS levels (P=0.001) in the CRVO patients. CONCLUSION: This is the first report of lowered serum PON1-ARE level as a risk factor for CRVO (OR= 1.108, CI=0.914, 1.314; P=0.296), which is found to correlate with oxidative stress.
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Arildialquilfosfatasa/sangre , Hidrolasas de Éster Carboxílico/sangre , Hiperhomocisteinemia/complicaciones , Oclusión de la Vena Retiniana/enzimología , Oclusión de la Vena Retiniana/etiología , Adulto , Estudios de Casos y Controles , Femenino , Humanos , Hiperhomocisteinemia/enzimología , Masculino , Estrés Oxidativo , Oclusión de la Vena Retiniana/sangre , Factores de Riesgo , Sustancias Reactivas al Ácido Tiobarbitúrico/análisis , Adulto JovenRESUMEN
Hydrogen sulphide (H2S), a signaling gasotransmitter and a potent vasorelaxant is endogenously produced by the enzymes cystathionine-beta-synthase (CBS) and cystathionine-gamma-lyase (CSE). CBS is a predominant source of H2S in the central nervous system, while CSE is the major H2S producing enzyme in the brain and other nervous tissues. Though the expression of these enzymes in the blood lymphocytes is known, H2S formation in the lymphocytes has not been reported so far. In the present study, H2S levels in the lymphocytes of healthy control subjects were estimated, after suitable modifications in a routine method [Stipanuk M H & Beck P W (1982) Biochem J 206, 267-277] used for detecting tissue levels of H2S. In this method, homocysteine (Hcys) due to its higher solubility was used as the substrate in place of L-cysteine and NaOH was used in place of zinc acetate to increase the entrapment of H2S in the central well. A mean H2S level of 11.64 +/- 6.36 microM/min/mg protein was detected in the lymphocytes of 8 subjects (mean age, 24 +/- 2; 2 male, 6 female). The modified method was found to be more sensitive for H2S estimation in human lymphocytes. As endogenous H2S is reported to be involved in the pathogenesis of various cardiovascular and pulmonary diseases, the levels of H2S in lymphocytes can be a marker of the endogenous tissue levels.
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Sulfuro de Hidrógeno/análisis , Sulfuro de Hidrógeno/química , Linfocitos/metabolismo , Adulto , Cisteína/química , Femenino , Homocisteína/química , Humanos , Leucocitos Mononucleares/metabolismo , Linfocitos/citología , Masculino , Modelos Biológicos , Modelos Químicos , Transducción de Señal , Especificidad por Sustrato , Distribución Tisular , Acetato de Zinc/química , Acetato de Zinc/farmacologíaRESUMEN
A feasibility study was conducted to evaluate the efficiency of Pseudomonas fluorescens NS1 bioaugmented to stimulate in situ bioremediation of crude oil-contaminated soil with different amendments in treatment units. Pure culture of P. fluorescens NS1 was isolated from a petroleum-contaminated soil. The rate of degradation of petroleum hydrocarbons by the indigenous soil microflora and in the presence of P. fluorescens NS1 was assessed with the addition of nutrients and bulking agents for a period of about 35 days. The study showed that addition of wheat bran as bulking agent rapidly enhanced bioremediation of crude oil-contaminated soil compared to amendments in other treatment units.
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Petróleo/metabolismo , Pseudomonas fluorescens/fisiología , Contaminantes del Suelo/metabolismo , Biodegradación Ambiental , Fibras de la Dieta , Contaminación Ambiental/prevención & control , Dinámica Poblacional , Microbiología del SueloRESUMEN
A strain of Pseudomonas fluorescens, isolated from petroleum hydrocarbon-contaminated soil was examined for its ability to utilize a variety of hydrocarbon substrates. Surface tension measurements indicated the production of biosurfactant during the microbial degradation of hydrocarbon. The organism utilized both short and long chain n-alkanes. It emulsified a number of aliphatic and aromatic hydrocarbons.