Effects of dietary yeast mannan-rich fraction supplementation on growth performance, intestinal morphology, and lymphoid tissue characteristics in weaned piglets challenged with Escherichia Coli F4.

We evaluated the effects of supplementing yeast mannan-reach-fraction on growth performance, jejunal morphology and lymphoid tissue characteristics in weaned piglets challenged with E. Coli F4. A total of 20 crossbred piglets were used. At weaning, piglets were assigned at random to one of four groups: piglets challenged and fed the basal diet supplemented with yeast mannan-rich fraction (C-MRF, n = 5); piglets challenged and fed the basal diet (C-BD, n = 5); piglets not challenged and fed the basal diet supplemented with yeast mannan-rich fraction (NC-MRF, n = 5), and piglets not challenged and fed the basal diet (NC-BD). Each dietary treatment had five replicates. On days 4, 5 and 10, piglets were orally challenged with 108 CFU/mL of E. Coli F4. C-MRF piglets had higher BW (p = 0.002; interactive effect) than C-BD piglets. C-MRF piglets had higher (p = 0.02; interactive effect) ADG in comparison with C-BD piglets. C-MRF piglets had higher (p = 0.04; interactive effect) ADFI than C-BD piglets. The diameter of lymphoid follicles was larger (p = 0.010; interactive effect) in the tonsils of C-MRF piglets than C-BD piglets. Lymphoid cells proliferation was greater in the mesenteric lymphnodes and ileum (p = 0.04 and p = 0.03, respectively) of C-MRF piglets. A reduction (p > 0.05) in E. Coli adherence in the ileum of piglets fed MRF was observed. In conclusion, the results of the present study demonstrate that dietary yeast mannan-rich fraction supplementation was effective in protecting weaned piglets against E. Coli F4 challenge.

Potential of calcium nitrate to mitigate the aluminum toxicity in Phaseolus vulgaris: effects on morphoanatomical traits, mineral nutrition and photosynthesis.

Common bean (Phaseolus vulgaris) cultivation occurs mainly in regions with acidic soils, where high aluminum (Al) concentration is a major constraint to crop production. In this study, we evaluated tolerance and sensitivity traits to Al exposure and calcium (Ca) deficiency in bean plants, and determined the efficiency of Ca to mitigate the toxic Al effects. Two bean cultivars (BRS Estilo and Campos Gerais) were grown in three soil conditions: (I) soil liming with calcium hydroxide Ca(OH)2 and Al unavailable (-Al+Ca); (II) fertilized soil with calcium nitrate [Ca(NO3)2·4H2O] and Al available (+Al+Ca); and (III) soil without Ca addition and Al available (+Al-Ca). At the beginning of the reproductive stage, we evaluated the photosynthetic processes, mineral nutrition, and leaf anatomy and morphological traits of plants. The photosynthetic parameters were good tools for monitor Al sensitivity in bean. +Al+Ca soil treatment mitigated the deleterious effects of Al on growth and mineral nutrition of both bean cultivars. However, Ca did not prevent the toxic effects of Al on leaf anatomy. Al stress and Ca deficiency caused negative effects on nutrient content, photosynthetic activity and leaf anatomy of bean plants. Calcium mitigated Al toxicity, primarily in the Campos Gerais cultivar, showing the potential to improve bean crop productivity in acid soils.

Citopatologia cervical e perfil epidemiológico de mulheres com vida sexual ativa / Cervical cytopathology and epidemiologic profile of women with sexual active life / Citopatologia cervical y perfil epidemiológico de mujeres con vida sexual activa

Objetivo: caracterizar as alterações citopatológicas cervicais e o perfil epidemiológico de mulheres com vida sexual ativa. Método: estudo quantitativo, descritivo, analítico, de corte transversal e base documental retrospectiva, realizado no município de Campo Grande/MS. Foram analisados os resultados dos exames citopatológicos referentes ao período de janeiro de 2010 a 2011. Os dados foram coletados com instrumento próprio. O projeto de pesquisa teve a aprovação pelo Comitê de Ética em Pesquisa, protocolo nº 063/11. Resultados: obteve-se a amostra de 527 exames. As alterações citopatológicas foram encontradas nas pacientes mais jovens, com menor nível de escolaridade. A afecção mais encontrada foi a Inflamação (22,5%) e os agentes mais comuns foram Cocos, Bacilos e Lactobacilos. Conclusão: os profissionais de saúde devem ser preparados para atender o público feminino em suas especificidades, bem como estimular a realização do exame Papanicolaou.(AU)

Objective: to characterize the cervical cytopathological changes and the epidemiological profile of women with sexual active life. Method: quantitative, descriptive, analytical study, cross-sectional and retrospective documentary base, held in the city of Campo Grande/MS. The test results were analyzed for the period from January 2010 to 2011. Data were collected with the instrument. The research project was approved by the Research Ethics Committee, protocol number 063/11. Results: the sample was obtained from 527 tests. The cytological changes were found in younger patients with less education. The most frequent disease was inflammation (22.5%), and the most common agents were Cocci, Bacilli and Lactobacilli. Conclusion: health professionals should be prepared to meet the women in their specificities and stimulate the realization of the pap smear.(AU)

Objetivo: caracterizar las alteraciones citopatológicas cervicales y el perfil epidemiológico de mujeres con vida sexual activa. Método: estudio cuantitativo, descriptivo, analítico, de cohorte transversal y base documental retrospectiva, realizado en la ciudad de Campo Grande/MS. Fueron analizados los resultados de los exámenes citopatológicos referentes al período de enero de 2010 a 2011. Los datos fueron recogidos con instrumento propio. El proyecto de investigación fue aprobado por el Comité de Ética en Investigación, protocolo nº 063/11. Resultados: se obtuvo la muestra de 527 exámenes. Las alteraciones citopatológicas fueron encontradas en las pacientes más jóvenes, con menor nivel de escolaridad. La afección más encontrada fue la Inflamación (22,5%), y los agentes más comunes fueron Cocos, Bacilos y Lactobacilos. Conclusión: los profesionales de salud deben ser preparados para atender al público femenino en sus especificidades, bien como estimular la realización del examen Papanicolaou.(AU)

Interação entre herbicidas e cultivares de soja sobre o nematoide de cisto Heterodera glycines / Interaction between herbicides and soybean cultivars on the cyst nematode Heterodera glycines

O nematóide de cisto, Heterodera glycines, encontra-se disseminado por todas as regiões brasileiras produtoras de soja. Embora medidas para reduzir a população desse parasito sejam bem conhecidas pouco se sabe sobre o efeito de herbicidas e sua interação com cultivares suscetíveis e resistentes, ou mesmo transgênicas, sobre o nematóide. Com este objetivo foram conduzidos dois experimentos na safra 2006/2007, em áreas naturalmente infestadas, nos municípios de Campo Alegre de Goiás e Gameleira de Goiás, onde predominam, respectivamente, as raças 14 e 3 (Tipos 1,3-- e 0--) de H. glycines. O delineamento experimental utilizado foi o de blocos casualizados em esquema fatorial 4x4+1, com três repetições. Os tratamentos foram constituídos pela interação entre quatro cultivares de soja (BRSGO Ipameri, BRSGO Luziânia, BRSGO Jataí, BRS Silvânia RR) e quatro formas de controle de plantas daninhas (arranquio manual, chlorimuron-etil+lactofen, chlorimuron-etil e haloxyfop-metil), além do tratamento adicional representado pela combinação da cultivar transgênica BRS Silvânia RR e o herbicida glifosato. O uso de glifosato em cultivar transgênica não influenciou a população de H. glycines. Em Campo Alegre de Goias o uso de clorimuron + lactofen reduziu a população de H. glycines na cultivar BRSGO Silvânia RR aos 80 DAS e na cultivar BRSGO Jataí na avaliação final. A cultivar resistente BRSGO Ipameri não proporcionou redução na população do nematóide, mas apresentou maior desenvolvimento vegetativo, indicado por maior matéria seca, biomassa e índice de área foliar (IAF).

The soybean cyst nematode is spread in all soybean producer regions. Although methods for reducing the H. glycines population are well established, little is known about the effect of herbicides and its interaction with resistant and susceptible, or even genetically modified cultivars. With the purpose to evaluate these interactions two experiments were carried out in 2006/2007 agricultural year in two different fields naturally infested. Experiments were done in Campo Alegre de Goiás (HG Type 1,3--) and Gameleira de Goiás (HG Type 0--) using randomized block design with treatments arranged in a factorial scheme 4 x 4 + 1. Factor A was cultivars (BRSGO Ipameri, BRSGO Luziânia, BRSGO Jataí BRS Silvânia RR) and factor B weed control (hand weeding, chlorimuron-ethyl + lactofen, chlorimuronethyl and haloxyfop-methyl). The additional treatment was the combination of genetically modified cultivar BRS Silvania RR and herbicide glyphosate. The use of glyphosate on cultivar BRS Silvania did not affect H. glycines population. In Campo Alegre de Goiás clorimuron + lactofen reduced the nematode population on soybean cultivar BRSGO Silvania RR at 80 DAS and the final population on soybean cultivar BRSGO Jatai. The resistant cultivar BRSGO Ipameri did not reduce the nematode population, but increase the plant development as indicated by higher dry weight, biomass and LAI.

Efeito de fungicidas e herbicidas no controle de Sclerotinia sclerotiorum / Effect of fungicides and herbicides in the control of Sclerotinia sclerotiorum

O objetivo deste trabalho foi estudar o efeito de fungicidas e herbicidas sobre o crescimento micelial de S. sclerotiorum, e o efeito preventivo e curativo de fungicidas no controle da podridão branca da haste da soja. No experimento in vitro, vinte e oito fungicidas e quatro herbicidas, na concentração de 100 µg.i.a.mL-1, foram incorporados ao meio BDA e avaliados sobre o crescimento micelial de dois isolados. No experimento em campo, os fungicidas fluazinam, epoxiconazol+piraclostrobina, tebuconazol+trifloxistrobina, tebuconazol, ciproconazol+propiconazol, procimidona, iprodiona, vinclozolin, protioconazol, azoxistrobina+ciproconazol e tiofanato metílico, foram testados preventiva e curativamente no controle da doença. Com exceção de azoxistrobina, clorothalonil, pencycuron, quintozeno, tiofanato metílico, carboxin+thiram e carbendazim, todos os outros fungicidas inibiram entre 98% a 100% do crescimento micelial dos dois isolados. Dentre os herbicidas, clorimurom-etílico reduziu em 79,4% e 84,1% o crescimento micelial dos isolados. Os fungicidas protioconazol, iprodiona, ciproconazol+propiconazol, fluazinam, vinclozolin e epoxiconazol+piraclostrobina proporcionaram menor severidade da doença quando aplicados preventivamente. Em aplicações curativas, apenas iprodione destacou-se na redução da doença.

This study evaluated the effect of fungicides and herbicides on mycelial growth of S. sclerotiorum, and the preventive and curative effects of fungicides on the control of soybean stem white rot. Twenty eight fungicides and four herbicides were used in the in vitro experiment, at the concentration of 100 µg a.i. mL-1, incorporated into the medium PDA, and evaluated on the mycelial growth of two isolates. The fungicides fluazinam, epoxiconazole+piraclostrobine, tebuconazole+trifloxistrobine, tebuconazole, ciproconazole+propiconazole, procimidone, Biosci. J., Uberlândia, v. 29, n. 6 , p. 1989-1996, Nov./Dec. 2013 iprodione, vinclozolin, protioconazole, azoxistrobine+ciproconazole and methyl thiophanate were tested in the field for the preventive and curative control of the disease. Except for the fungicides azoxistrobine, clorothalonil, pencycuron, quintozene, methyl thiophanate, carboxin+thiram and carbendazim, all others inhibited from 98% to 100% of the mycelial growth of both isolates. Among the herbicides, clorimurom-ethyl reduced from 79.4% to 84.1% the growth of both isolates. The fungicides protioconazol, iprodione, ciproconazole+propiconazole, fluazinam, vinclozolin and epoxiconazole+piraclostrobine provided lower disease severity in prventive application; in contrast, only iprodione was effective in curative effect of disease reduction.

Atividade antifúngica de óleo e extratos vegetais sobre Sclerotinia sclerotiorum / Antifungal activity of vegetable oils and extracts against Sclerotinia sclerotiorum

Considerando a importância do mofo branco, causado pelo patógeno Sclerotinia sclerotiorum na cultura da soja (Glycine max) e a falta de estudos sobre alternativas de controle deste patógeno, este trabalho teve como objetivo estudar o efeito de óleos e extratos vegetais sobre o crescimento micelial de S. sclerotiorum. No experimento de óleos essenciais, concentrações de 25, 50, 75 e 100 µg de i.a mL-1 de azadiractina, obtida de nim indiano (Azadirachta indica A. Juss), foram estudadas em associação às doses de 0, 1/3, 1/6, 1/8 e 1/10 do óleo de Karanja (Pongamia glabra). Quanto ao experimento de extratos vegetais, estudou-se as espécies Aroeirinha (Schinus molle L.), Mentrasto (Ageratum conyzoides L.), Alfavaca (Ocimum gratissimum L.), Losna (Artemisia absinthium L.), Jambolão (Syzygium cumini (L.) Skeels), Arruda (Ruta graveolens L.), Mandioca (Manihot esculenta Crantz), Santa Bárbara (Melia azedarach L.) e Pimenta longa (Piper aduncum L.) na concentração de 30%. Óleos e extratos vegetais foram incorporados, separadamente, ao meio de cultura BDA e vertido em placa de Petri de 9 cm de diâmetro, na qual depositou-se um disco de BDA, contendo micélio do fungo. As placas foram incubadas à temperatura de 22 ± 3 oC e fotoperíodo de 12 horas. As avaliações consistiram em medições diárias do diâmetro da colônia, durante 48 horas. A maior inibição do crescimento micelial foi diretamente proporcional ao aumento das concentrações dos óleos de nim indiano e de Karanja. A concentração de azadiractina, correspondente a 100 µg de i.a mL-1 com 1/3 de óleo de Karanja foi a mais eficiente na redução do crescimento micelial, com 63% de inibição. Em relação aos extratos vegetais, o fruto de pimenta longa foi o mais promissor sobre a redução do crescimento micelial, com 43% de inibição.

Considering the importance of white mold, caused by the pathogen Sclerotinia sclerotiorum, to soybeans (Glycine max) and the lack of studies about alternative control ways of plant pathogens, this study evaluated the effect of vegetable oils and extracts on the mycelial growth of S. sclerotiorum. Concentrations of 25, 50, 75 and 100 µg a.i. mL-1 of azadiractine, obtained from neem (Azadirachta indica A. Juss), were evaluated associated with the doses of 0, 1/3, 1/6, 1/8 or 1/10 of Karanja (Pongamia glabra) oil in the experiment with essential oils. Vegetable extracts of the species Schinus molle L., Ageratum conyzoides L., Ocimum gratissimum L., Artemisia absinthium L., Syzygium cumini (L.) Skeels, Ruta graveolens L., Manihot esculenta Crantz, Melia azedarach L. and Piper aduncum L. were evaluated in the concentration of 30%. Oils and vegetable extracts were incorporated, separately, into PDA culture medium and poured in 9-cm diameter petri plates, over which a PDA disk containing mycelium of the fungus was placed. The plates were incubated at 22 ± 3 oC and 12 hours lighting. The evaluations consisted of daily measurements of colony diameter for two days. The greatest mycelial growth inhibition was directly proportional to the concentration increase of neem and Karanja oils. Azadiractin concentration corresponding to 100 µg a.i. mL-1 with 1/3 Karanja oil was the most effective on reducing mycelial growth, with 63% inhibition. Among the vegetable extracts, fruits of long pepper were the most promising for reducing mycelial growth, with 43% inhibition.