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1.
Protein Pept Lett ; 28(7): 761-768, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33302826

RESUMEN

BACKGROUND: The microbiome is now known for its important role in whole-body homeostasis. A dysbiosis of the normal microbiota is correlated with metabolic disorders. In this sense, the search for compounds able to modulate the microbiome is needed. Resveratrol, a natural compound found in grapes seems to be a promising candidate. OBJECTIVE: In this study, our motivation was to evaluate the effects of the association between Resveratrol and Lactococcus lactis, a probiotic, on the composition of the gastrointestinal microbiota and body weight of mice. METHODS: Twenty female mice were divided into 4 groups: (1) standard diet, (2) standard diet plus Lactococcus lactis, (3) standard diet plus resveratrol, and (4) standard diet plus Lactococcus lactis and resveratrol. At the end of the treatment period, samples of blood, mucus, stomach, and small and large intestines were collected for analysis. Total levels of Immunoglobulin A and Immunoglobulin E, Lac+ and Lac- bacteria and Lactobacillus were measured. RESULTS: The main results indicate that the association between resveratrol and probiotics was able to decrease mice body weight, as compared to the other groups, in addition to decrease the number of Lac- bacteria and increasing the number of Lac+ bacteria. The levels of secretory IgA were also decreased, compared to the animals treated with only probiotics or resveratrol. CONCLUSION: We observed potential synergism between Resveratrol and Lactococcus lactis mainly in modulating the stomach and intestinal microbiota.


Asunto(s)
Peso Corporal/efectos de los fármacos , Enterobacteriaceae/efectos de los fármacos , Microbioma Gastrointestinal/efectos de los fármacos , Lactococcus lactis/inmunología , Probióticos/administración & dosificación , Resveratrol/administración & dosificación , Animales , Peso Corporal/inmunología , Dieta/métodos , Enterobacteriaceae/crecimiento & desarrollo , Enterobacteriaceae/inmunología , Femenino , Microbioma Gastrointestinal/inmunología , Inmunoglobulina A/biosíntesis , Inmunoglobulina E/sangre , Intestino Grueso/efectos de los fármacos , Intestino Grueso/inmunología , Intestino Grueso/microbiología , Intestino Delgado/efectos de los fármacos , Intestino Delgado/inmunología , Intestino Delgado/microbiología , Ratones , Ratones Endogámicos C57BL , Estómago/efectos de los fármacos , Estómago/inmunología , Estómago/microbiología
2.
Protein Pept Lett ; 24(9): 784-792, 2017 Nov 17.
Artículo en Inglés | MEDLINE | ID: mdl-28814250

RESUMEN

INTRODUCTION: The Renin-Angiotensin System (RAS) has emerged as being related to vascular disease. Recently the RAS has been associated with obesity, diabetes, and even cancer. OBJECTIVE: This review and Bioinformatics analyses focuses on the investigation of Angiotensinconverting enzymes (ACE and ACE2) as therapeutical targets for Malignant Epithelial Neoplasia, specifically for Oral Squamous Cell Carcinoma (OSCC). CONCLUSION: The literature review and Bioinformatics analyses showed that ACE and ACE2 are interesting targets for OSCC treatment. Studies involving RAS and OSCC should be encouraged for experimental validation.


Asunto(s)
Inhibidores de la Enzima Convertidora de Angiotensina/farmacología , Antineoplásicos/farmacología , Carcinoma de Células Escamosas/tratamiento farmacológico , Neoplasias de la Boca/tratamiento farmacológico , Peptidil-Dipeptidasa A/metabolismo , Enzima Convertidora de Angiotensina 2 , Inhibidores de la Enzima Convertidora de Angiotensina/química , Inhibidores de la Enzima Convertidora de Angiotensina/uso terapéutico , Antineoplásicos/química , Antineoplásicos/uso terapéutico , Biología Computacional , Humanos , Terapia Molecular Dirigida , Neoplasias de la Boca/metabolismo , Peptidil-Dipeptidasa A/química , Sistema Renina-Angiotensina/fisiología
3.
Surg Obes Relat Dis ; 12(7): 1292-1299, 2016 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-27039133

RESUMEN

BACKGROUND: Visceral obesity has been considered a risk factor for metabolic and cardiovascular complications. In an attempt to reduce the visceral adipose tissue, omentectomy has been proposed to be performed along with bariatric surgery. OBJECTIVE: The goal of this study was to evaluate whether omentectomy associated with sleeve gastrectomy (SG) is beneficial to the inflammatory and metabolic profile of rats fed a standard diet (STD) or high-fat diet (HFD). SETTING: University hospital, Brazil. METHODS: For this experiment, male Wistar rats were randomly divided into 6 groups as follows: sham surgery (STD+L or HFD+L), SG alone (STD+SG or HFD+SG), or SG with omentectomy (STD+SGO or HFD+SGO). Anthropometric data and metabolic profiles were evaluated, and the tissue expression of inflammatory markers in the visceral adipose tissue was measured. RESULTS: In rats with diet-induced obesity treated with SG with or without omentectomy, there was a reduction in weight (HFD+SG: P<.01 and HFD+SGO: P<.05), adiposity (HFD+SG: P<.001 and HFD+SGO: P<.05), plasma levels of glucose (HFD+SG: P<.01 and HFD+SGO: P<.01), plasma levels of C-peptide (HFD+SG: P<.01 and HFD+SGO: P<.001), plasma levels of insulin (HFD+SG: P<.05 and HFD+SGO: P<.001), plasma levels of total cholesterol (HFD+SG: P<.01 and HFD+SGO: P<.01), and tissue expression of TNF-α (HFD+SG: P<.001 and HFD+SGO: P<.01), but there was no statistically significant difference between the groups in which omentectomy was performed or was not. CONCLUSION: In this study, we did not observe additional beneficial effects due to omentectomy associated with SG in the metabolic profile and tissue expression of inflammatory markers.


Asunto(s)
Cirugía Bariátrica/métodos , Gastrectomía/métodos , Obesidad Mórbida/cirugía , Epiplón/cirugía , Animales , Biomarcadores/metabolismo , Colesterol/metabolismo , Citocinas/metabolismo , Dieta Alta en Grasa , Grasa Intraabdominal/anatomía & histología , Masculino , Distribución Aleatoria , Ratas Wistar , Pérdida de Peso/fisiología
4.
Int J Mol Cell Med ; 5(4): 199-219, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-28357197

RESUMEN

Pathogenesis of odontogenic tumors is not well known. It is important to identify genetic deregulations and molecular alterations. This study aimed to investigate, through bioinformatic analysis, the possible genes involved in the pathogenesis of ameloblastoma (AM) and keratocystic odontogenic tumor (KCOT). Genes involved in the pathogenesis of AM and KCOT were identified in GeneCards. Gene list was expanded, and the gene interactions network was mapped using the STRING software. "Weighted number of links" (WNL) was calculated to identify "leader genes" (highest WNL). Genes were ranked by K-means method and Kruskal-Wallis test was used (P<0.001). Total interactions score (TIS) was also calculated using all interaction data generated by the STRING database, in order to achieve global connectivity for each gene. The topological and ontological analyses were performed using Cytoscape software and BinGO plugin. Literature review data was used to corroborate the bioinformatics data. CDK1 was identified as leader gene for AM. In KCOT group, results show PCNA and TP53. Both tumors exhibit a power law behavior. Our topological analysis suggested leader genes possibly important in the pathogenesis of AM and KCOT, by clustering coefficient calculated for both odontogenic tumors (0.028 for AM, zero for KCOT). The results obtained in the scatter diagram suggest an important relationship of these genes with the molecular processes involved in AM and KCOT. Ontological analysis for both AM and KCOT demonstrated different mechanisms. Bioinformatics analyzes were confirmed through literature review. These results may suggest the involvement of promising genes for a better understanding of the pathogenesis of AM and KCOT.

5.
Protein Pept Lett ; 22(4): 332-40, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-25666042

RESUMEN

Recent studies have shown that angiotensin-converting enzyme 2 (ACE2)/angiotensin (Ang) -(1-7)/Mas axis activation is able to improve the metabolic profile, enhance glucose tolerance and insulin sensitivity, improve metabolic parameters, and counteract deleterious effects of Ang II. The effects of endogenous ACE 2 activation on the metabolic profile of mice are poorly studied. In this study, 12 weeks old male mice were treated with the ACE 2 activator (diminazene aceturate, DIZE, 1 mg/kg/day, gavage) or saline (control) for 30 days followed by glucose tolerance tests, insulin sensitivity tests, and blood analysis. Epididymal ACE2, ACE, angiotensinogen, acetyl-CoA carboxylase (ACC), and fatty acid synthase (FAS) were measured by quantitative RT-PCR. ACE 2 activation treatment lowered body weight (DIZE vs control) (28.69 vs 30.28g, P < 0.001), serum cholesterol (140,0 vs 177.5; P < .05), and serum triglycerides (75,00 vs 165,0; P < .05) as well as epididymal (0.008 vs 0.016; P < .05) and retroperitoneal (0.0024 vs. 0.0068; P < .01) adipose tissue weights. These effects were associated with significantly increased epididymal ACE 2 and decreased ACE and angiotensinogen (AGT) expression. Additionally, DIZE decreased adipogenesis-related gene transcription, such as ACC and FAS mRNA. In conclusion, these results indicate that activation of ACE2 by oral DIZE treatment improves the metabolic profile and reduces fat deposition in mice. These results, along with the reduction of lipogenesis markers open a new perspective for metabolic disorder pharmacotherapy.


Asunto(s)
Diminazeno/análogos & derivados , Inhibidores Enzimáticos/metabolismo , Lipogénesis/efectos de los fármacos , Peptidil-Dipeptidasa A/metabolismo , Transcriptoma/efectos de los fármacos , Tejido Adiposo/efectos de los fármacos , Enzima Convertidora de Angiotensina 2 , Animales , Peso Corporal/efectos de los fármacos , Diminazeno/metabolismo , Diminazeno/farmacología , Inhibidores Enzimáticos/farmacología , Humanos , Masculino , Ratones , Proteínas Proto-Oncogénicas p21(ras)/metabolismo
6.
J Endod ; 39(4): 453-5, 2013 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-23522535

RESUMEN

INTRODUCTION: Chronic dental periapical lesions result from chronic inflammation of periapical tissues caused by continuous antigenic stimulation from infected root canals. Recent findings have suggested that T helper (Th) 1 and Th2-like cytokines are important in the pathogenesis of chronic periapical inflammatory diseases. However, the mechanisms regulating these immunoinflammatory pathways have not been fully elucidated. Thus, the aim of this study was to evaluate interleukin (IL)-4, IL-12, and interferon γ (IFN-γ) protein levels in human radicular cysts and periapical granulomas. METHODS: Archived samples of cysts (n = 52) and granulomas (n = 27) were sectioned and submitted to immunohistochemistry to evaluate the tissue expression of IL-4, IL-12, and IFN-γ. The data were analyzed using the Mann-Whitney U test (P < .05). RESULTS: An increased expression of IFN-γ was observed in radicular cysts. IL-4 expression was stronger in periapical granulomas than in radicular cysts. IL-12 was not detected in any of the samples. CONCLUSIONS: Our study showed that IFN-γ protein levels are increased in radicular cysts, whereas IL-4 expression is stronger in samples of periapical granulomas. Further studies are necessary to elucidate the signaling pathways mediated by these cytokines and to facilitate the development of more effective periapical disease management strategies.


Asunto(s)
Interferón gamma/metabolismo , Interleucina-4/metabolismo , Granuloma Periapical/inmunología , Quiste Radicular/inmunología , Balance Th1 - Th2 , Adulto , Estudios Transversales , Femenino , Humanos , Interleucina-12/metabolismo , Masculino , Persona de Mediana Edad , Estadísticas no Paramétricas , Adulto Joven
7.
Artículo en Inglés | MEDLINE | ID: mdl-17178493

RESUMEN

OBJECTIVE: White sponge nevus (WSN) is a rare autosomal dominant disorder that results in soft, white, and spongy plaques in the oral mucosa. The aim of this study was to describe the clinical, histopathologic, and genetic features of a family, spanning 3 generations, affected by WSN. STUDY DESIGN: This study was performed using a cross-sectional layout analyzing a family with WSN. RESULTS: Clinical examination of family members revealed that of 23 descendants, 8 (34.78%) had WSN features. Unaffected and affected members transmitted the disease to their offspring. The offspring recurrence risk was 0.34, and an incomplete level of penetrance was observed. The lesions showed many clinical and histopathologic similarities to cases previously reported. The most affected sites were buccal and labial mucosa, with a rare appearance in the palate. No extraoral lesion was found. Histological examination showed intense acanthosis and hyperparakeratosis-induced epithelial hyperplasia. Within the spinous layer, cells showing perinuclear eosinophilic condensation of the cytokeratin (CK) filaments were frequent. CONCLUSION: The disease was transmitted by an autosomal dominant mode of inheritance, appearing mainly in the buccal and labial mucosa.


Asunto(s)
Familia , Leucoqueratosis Mucosa Hereditaria/genética , Enfermedades de la Boca/genética , Estudios Transversales , Femenino , Genes Dominantes , Humanos , Leucoqueratosis Mucosa Hereditaria/patología , Masculino , Enfermedades de la Boca/patología , Mucosa Bucal/patología , Linaje , Penetrancia
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