RESUMEN
Phosphorus (P) nutrition of beech ecosystems depends on soil processes, plant internal P cycling and P acquisition. P uptake of trees in the field is currently not validated due to the lack of an experimental approach applicable in natural forests. Application of radiolabelled tracers such as 33 P and 32 P is limited to special research sites and not allowed in natural environments. Moreover, only one stable isotope of P, namely 31 P, exists. One alternative tool to measure P acquisition in the field could be the use of 18 O-labelled 31 P-phosphate (31 P18 O4 3- ). Phosphate (Pi ) uptake rates calculated from the 18 O enrichment of dried root material after application of 31 Pi 18 O4 3- via nutrient solution was always lower compared to 33 P incorporation, did not show increasing rates of Pi uptake at P deficiency under controlled conditions, and did not reveal seasonal fluctuations in the field. Consequently, a clear correlation between 33 P-based and 18 O-based Pi uptake by roots could not be established. Comparison of Pi uptake rates achieved from 33 P-Pi and 18 O-Pi application led to the conclusion of high Pi metabolism in roots after Pi uptake. The replacement of 18 O by 16 O from water in 18 O-Pi during root influx, but most probably after Pi uptake into roots, due to metabolic activities, indicates high and fast turnover of Pi . Hence, the use of 18 O-Pi as an alternative tool to estimate Pi acquisition of trees in the field must consider the increase of 18 O abundance in root water that was disregarded in dried root material.
