A guide to facilitate the creation of a femoral tunnel for arthroscopic ligamentum teres reconstruction: a three-dimensional computed tomography study.

The ideal femoral tunnel passing through the centre of the femoral neck targeted to the footprint of the ligamentum teres (LT) is established during the LT reconstruction surgery with the free-hand technique. We aimed to quantitatively determine the entry site and define the angular orientation of the ideal femoral tunnel with its relevance to the femoral valgus angle (FVA) and the femoral anteversion angle (FAA) to facilitate the creation of an ideal femoral tunnel during the LT reconstruction surgery. A total of 60 randomly selected CT images were obtained to reconstruct three-dimensional femur models. A virtual reamer representing the ideal femoral tunnel was placed in the femur models. The femur length, FVA, FAA, the femoral tunnel anterior angle, the femoral tunnel superior angle and the skin- and bony-entry sites were measured. The femoral tunnel angular orientation was strongly correlated with the FVA and the FAA. Mathematical formulas were defined by which entry site of the reamer and the anterior and superior angulation of the femoral tunnel could be estimated before the surgery. The mean skin-entry site was 67.3 mm distal and 0.1 mm anterior to the centre of the greater trochanter's superior border. The angular orientation of the femoral tunnel using FVA and FAA can be easily estimated using mathematical formulas before LT reconstruction surgery. The entry site and angular orientation of the femoral tunnel described in this study can be used to reduce dependency on the usage of fluoroscopy and the workload on the surgeon during the LT reconstruction surgery.

Hair diameter measurements for planning follicular unit extraction surgery (FUE): Is there a correlation between the micrometer caliper and scanning electron microscopy (SEM) findings?

BACKGROUND: Hair shaft diameter is one of the most important factors for the outcome of follicular unit extraction (FUE) surgery. In fact, the hair shaft is elliptical. Therefore, it has a long and short axis. Many hair transplantation surgeons use manual micrometer caliper for gauging hair diameter and use the results in the management of recipient and donor area. AIM: In this study, we aimed to identify the dependability of micrometer caliper and also the hair diameter diversity pattern in the donor area. PATIENTS/METHODS: Two hundred and seventy hairs were collected from three males with androgenetic alopecia. Hair samples were obtained from the 1 cm2 boxes from superior to inferior at the mid-point of temporal, parietal, and occipital donor areas. The diameter of each hair was measured both with a micrometer caliper and scanning electron microscopy (SEM). RESULTS: Average diameter measured by scanning electron microscopy was 83.01 µm for the long axis and 51.51 µm for the short axis. The average value for the micrometer caliper measurement was 53.32 µm. Comparison of micrometer caliper results with the short-axis measurements of SEM revealed a strong significant correlation. The hair diameters from superior, middle, and inferior boxes revealed a tendency to decrease toward the inferior regions. CONCLUSIONS: A manual micrometer caliper is a dependable tool for planning FUE surgery, and it measures the short axis of the elliptical hair shaft. Hair diameter tends to decrease toward the inferior regions of the donor area.

Iliocapsularis muscle in human fetuses.

PURPOSE: To determine the incidence of the iliocapsularis muscle in fetal period and its relationship with the hip joint capsule. METHODS: Twenty-one formalin-fixed fetuses (12 female and 9 male) with a mean gestational age of 29 ± 3.89 weeks (range 25-36) were dissected to reveal morphological properties of iliocapsularis muscle. RESULTS: Iliocapsularis muscle was observed in 39 out of 42 sides (92%). Its proximal attachment was detected either below the proximal attachment of rectus femoris muscle in 21 out of 39 sides (54%), or it was forming a common tendon with rectus femoris on the anterior inferior iliac spine in 10 out of 39 sides (26%), or it was forming an arch along the superior-medial-inferior sides of the proximal attachment of rectus femoris muscle in 8 out of 39 sides (20%). Muscle fibers originating from the anteromedial part of hip joint capsule were also constant in all sides. Distal attachment of iliocapsularis muscle was distal to the lesser trochanter in all specimens. Its form was as a broad muscle in 32 out of 39 sides (82%) and as a thin rectangular muscular slip in 7 out of 39 sides (18%). Iliopsoas and iliocapsularis muscles had their particular fascia in 34 out of 39 sides (87%), and in the other 5 sides, there was no fascia which prominently separated the two muscles. Its length was longer in females than males (p = 0.031) and it was wider on the right side (p = 0.029). Linear functions were y = 0.49 + 0.17 × weeks and y = 6.94 + 0.89 × weeks for width and length, respectively. CONCLUSION: Data obtained with the present study about iliocapsularis muscle in fetal period revealed that it is an individual and constant muscle. Its dimension, location, and course over the hip joint capsule support the idea that it tightens the hip capsule and stabilizes the femoral head.

Soft spot: the important zone at the standard posterior portal of shoulder arthroscopy

Background/aim: The localization of the standard posterior portal of shoulder arthroscopy and landmarks mentioned in the literature are unclear. The purpose of this prospective cadaveric study was to determine the localization of the standard posterior portal and its distance to the neural structures. Materials and methods: One fresh frozen and 10 formalin-fixed adult cadaveric shoulders were dissected. In the beach chair position, a 5-mm trocar was placed anteroposteriorly from the superior edge of the subscapularis muscle, superior to the tip of the coracoid process and tangent to the glenoid. The relevant distances of the posterior exit point were measured. Results: In all specimens, the exit point was a triangular fibrous area, between the posterior and lateral parts of the deltoid. Medial and inferior distances of the trocar to the posterolateral tip of the acromion were 1.88 ± 0.53 cm and 1.35 ± 0.34 cm and distances to the axillary and suprascapular nerves were 4.54 ± 1.08 cm and 2.54 ± 0.85 cm, respectively. Conclusion: The most important finding of this study was the superficial localization of the soft spot between the posterior and lateral parts of deltoid.

Drilling through lateral transmuscular portal lowers the risk of suprascapular nerve injury during arthroscopic SLAP repair.

PURPOSE: The aim of our study was to evaluate the risk of medial glenoid perforation and possible injury to suprascapular nerve during arthroscopic SLAP repair using lateral transmuscular portal. METHODS: Ten cadaveric shoulder girdles were isolated and drilled at superior glenoid rim from both anterior-superior portal (1 o'clock) and lateral transmuscular portal (12 o'clock) for SLAP repairs. Drill hole depth was determined by the manufacturer's drill stop (20 mm), and any subsequent drill perforations through the medial bony surface of the glenoid were directly confirmed by dissection. The bone tunnel depth and subsequent distance to the suprascapular nerve, scapular height and width, were compared for investigated locations. RESULTS: Four perforations out of ten (40 %) occurred through anterior-superior portal with one associated nerve injury. One perforation out of ten (10 %) occurred through lateral transmuscular portal without any nerve injury. The mean depth was calculated as 17.6 mm (SD 3) for anterior-superior portal and 26.5 mm (SD 3.6) for lateral transmuscular portal (P < 0.001). CONCLUSIONS: It is anatomically possible that suprascapular nerve could sustain iatrogenic injury during labral anchor placement during SLAP repair. However, lateral transmuscular portal at 12 o'clock drill entry location has lower risk of suprascapular nerve injury compared with anterior-superior portal at 1 o'clock drill entry location.

Comparison of radiological and morphologic assessments of myocardial bridges.

In this study we aimed to compare the findings of coronary dual-source computed tomography angiography of myocardial bridges with cadaveric dissections. Forty-one isolated, non-damaged fresh sheep hearts were used in this study. Myocardial bridges of the anterior interventricular branch of the left coronary artery were demonstrated and analyzed by a coronary dual-source computed tomography angiography. Dissections along the left anterior interventricular branch of the left coronary artery were performed by using Zeiss OPMI pico microscope and the length of the bridges were measured. The depths of the myocardial bridges were measured from the stained sections by using the light microscope (Leica DM 6000B). MBs were found in all 41 hearts (100%) during dissections. Dual-source computed tomography angiography successfully detected 87.8% (36 of the 41 hearts) of the myocardial bridges measured on left anterior interventricular branch of left coronary artery. The lengths of the myocardial bridges were found 5-40 and 8-50 mm with dissection and dual-source computed tomography angiography, respectively. And the depths were found 0.7-4.5 mm by dual-source computed tomography angiography and 0.745-4.632 mm morphologically. Comparison of the mean values of the lengths showed statistically significantly higher values (22.0 ± 8.5, 17.7 ± 7.7 mm, p = 0.003) for the dissections. Radiological assessment also effectively discriminated complete bridges from incomplete ones. Our study showed that coronary computed tomography angiography is reliable in evaluating the presence and depth of myocardial bridges.

Differential diagnosis in a professional basketball player with foot pain: is it an avulsion fracture or an os supranaviculare?

The os supranaviculare is an accessory bone located on the dorsal aspect of the talonavicular joint close to the midpoint. This rare incidental skeletal variant has an estimated prevalence of 1%. It may rarely become symptomatic and should not be confused with cortical avulsion fractures of navicular or talar head. We present the case of a 25-year-old professional basketball player with pain on the dorsum of his right foot after twisting his ankle during a regular season match. Magnetic resonance imaging findings of the player's foot represented a flake of bone on the superior part of the talar head. The differential diagnosis and clinical outcome of this unusual case are briefly discussed.

Minimally invasive plating of the distal tibia: do we really sacrifice saphenous vein and nerve? A cadaver study.

OBJECTIVE: To investigate the risk of saphenous nerve (SN) and great saphenous vein (GSV) injury during percutaneous screw placement of the 3.5/4.5 LCP Distal Tibial Metaphyseal Plate and the 3.5-mm LCP Medial Distal Tibia Plate with tab in distal tibias of cadaver extremities. METHODS: Thirty-one unpaired (1 fresh and 30 formalin fixed) adult cadaveric lower extremity specimens were dissected. Using the principles of minimally invasive plating, a 3.5/4.5 LCP Distal Tibial Metaphyseal Plate was implanted in 16 extremities and a 3.5-mm LCP Medial Distal Tibia Plate with tab in the remaining 15 extremities. Injuries to or any evidences of direct contact with the SN or GSV were recorded. Additionally, the shortest distances of each hole to the main branches of these anatomic structures were measured. RESULTS: The risk of injury to the SN and GSV was higher in holes 4, 5, and 6 when using the 3.5/4.5 LCP Distal Tibial Metaphyseal Plate and in holes 3, 5, and 8 when using the 3.5-mm LCP Medial Distal Tibia Plate. CONCLUSIONS: The SN and GSV are at high risk for injury during percutaneous screw placement of the 3.5/4.5 LCP Distal Tibial Metaphyseal Plate and the 3.5-mm LCP Medial Distal Tibia Plate at the distal tibia. Careful dissection in the stab incisions down the plate, atraumatic placement of the drill sleeves, and protection of the soft tissues during screw insertion might decrease the risk of injury to the SN and GSV.

Rotator interval dimensions in different shoulder arthroscopy positions: a cadaveric study.

The rotator interval was defined as a triangular structure, where the base of the triangle was the coracoid base, the upper border was the anterior margin of the supraspinatus, and the lower border was the superior margin of the subscapularis muscle-tendon unit. We evaluated the rotator interval dimensions in 15 shoulders from 10 lightly embalmed adult cadavers in 3 shoulder arthroscopy positions: 0 degrees of abduction and 30 degrees of flexion (beach chair [BC]), 45 degrees of abduction and 30 degrees of flexion (lateral decubitus 1), and 70 degrees of abduction and 30 degrees of flexion (lateral decubitus 2). In each shoulder position, measurements were made in neutral rotation (NR), 45 degrees of external rotation (ER), and 45 degrees of internal rotation (IR). The coracoid base lengthened with IR in all positions and shortened in ER in the lateral decubitus position but not in the BC position. Abduction significantly lengthened the coracoid base, which was shortest in the BC position with ER (24 +/- 4 mm) and longest in the lateral decubitus 2 position with IR (33 +/- 5 mm). The coracoid base, where sutures are placed during plication of the interval, was observed to lengthen and, therefore, loosen with IR and abduction. To prevent postoperative ER restriction, plication should be made in ER or neutral rotation when operating in the BC position and the degree of abduction should be decreased and the shoulder held in ER when operating in the lateral decubitus position.

The safe distance for the superior gluteal nerve in direct lateral approach to the hip and its relation with the femoral length: a cadaver study.

INTRODUCTION: The most inferior branch (MIB) of the superior gluteal nerve (SGN) is vulnerable during direct lateral approach to the hip. A safe distance proximal to the tip of the greater trochanter varying from 3 to 5 cm has been reported in different studies. Anatomical studies defining safe zones and clinical studies reporting the results use various reference points, and the oblique course of the MIB contributes to the confusion. Numerous efforts have been made to standardize the safe zone using patient characteristics such as body height; however, contradictory results have been reported. The purpose of this study was to measure the safe distance in line to the gluteal split and also to determine the relationship of the safe distance with femoral length, as a stable component of body height. MATERIALS AND METHODS: Fifteen lower extremities of 12 formalin-fixed cadavers (M/F: 7/5) were dissected. The most prominent lateral palpable part of the trochanter major (TM) was determined and the dissection in the gluteus medius muscle (GMM) was performed starting from this point upwards in line of the muscle fibers. The distances between the MIB in the plane of dissection in the GMM to the TM and also to the trochanteric apex (TA) were measured. Femoral lengths were measured between the TM point and the lateral epicondyle. Spearman's correlation and Mann-Whitney U tests were used for statistical analysis. RESULTS: The SGN in 13 hips had spray pattern and neural trunk pattern in two. The plane of dissection was within the anterior third of the GMM in all hips. The average femoral length was 37.5 cm. Average distance between TM and MIB was 44 mm; in three hips, the distance was <30 mm. The average distance between TA and TM was 21 mm. There was no statistically significant correlation between femoral length and TM-MIB distance. CONCLUSION: The distance from the TM to the MIB is highly variable and independent from body height or femoral length. The so called "safe zone" in which damage of significant nerve damage is excluded can have a rather small dimension in some patients. Short patients are not at increased risk and tall patients are not risk free. Modern techniques in total hip replacement which try to minimize proximal interruption of the GMM are therefore justified.

Risk of superior gluteal nerve and gluteus medius muscle injury during femoral nail insertion.

BACKGROUND: Abduction weakness and limping is a well-recognized complication of closed antegrade insertion of femoral nails. Iatrogenic injuries to the superior gluteal nerve and the gluteus medius muscle are the most likely contributing factors. The purpose of this study of cadavers was to assess the risk of nerve and muscle injury with various lower-limb positions used during nail insertion. METHODS: We studied thirteen hips of ten formalin-fixed adult cadavers. With the cadaver in the full lateral position, a 9-mm reamer was introduced in a retrograde fashion from the intercondylar notch and passed through the gluteus medius muscle. The distance between the point of entry of the reamer into the undersurface of this muscle and the inferior main branch of the superior gluteal nerve (the nerve-reamer distance) and the distance between the entry and exit points of the reamer in the gluteus medius muscle (the intramuscle distance) were measured in three different hip positions: 15 degrees of flexion and 15 degrees of adduction (Position 1), 30 degrees of flexion and 30 degrees of adduction (Position 2), and 60 degrees of flexion and 30 degrees of adduction (Position 3). RESULTS: In Position 1, the average nerve-reamer distance was 7 mm and the average intramuscle distance was 24 mm. In three hips the reamer injured the nerve directly, and in two other hips the distance was

Clarithromycin destroys biofilms and enhances bactericidal agents in the treatment of Pseudomonas aeruginosa osteomyelitis.

In this study, the effect of clarithromycin on the destruction of bacterial biofilm in Pseudomonas aeruginosa osteomyelitis was investigated. Foreign body-related osteomyelitis caused by ceftazidime-sensitive Pseudomonas aeruginosa was produced in the tibias of 26 rats. After osteomyelitis was verified on Day 14, 10 rats had ceftazidime (1500 mg/kg/day) given subcutaneously, and 10 rats had ceftazidime given subcutaneously and clarithromycin (100 mg/kg/day, two 50-mg/kg doses every 12 hours) given orally; three rats formed the control group. After a treatment period of 20 days, the tibias and the foreign bodies were removed, cultured, and examined by electron microscopy. The number of microorganisms growing on the bone tissue in the group receiving combined treatment was significantly lower than in the other groups. The number of microorganisms growing on the foreign body in the group receiving only ceftazidime was significantly higher than that of the group receiving combined treatment. Electron microscope examination revealed that the biofilm layer was eradicated in the group that had combined therapy; however, biofilm formation was evident on the foreign body in the group receiving only ceftazidime. Clarithromycin enhanced the activity of concomitantly used bactericidal agents by destroying biofilm on the surface of the materials.

Recesses along the posterior margin of the infrapatellar (Hoffa's) fat pad: prevalence and morphology on routine MR imaging of the knee.

The objective is to evaluate the prevalence and morphology of recesses along the posterior margin of the infrapatellar fat pad on routine MR imaging of the knee. MR images of 213 knees in 204 consecutive individuals were evaluated with regard to the prevalence and morphology of recesses (a "suprahoffatic" recess close to the inferior border of the patella and the previously described "infrahoffatic" recess anterior to the inferior portion of the infrapatellar plica). The recesses were analyzed with regard to synovial effusion and the condition of the anterior cruciate ligament (ACL). Anatomic dissection was made in 29 knees in 16 cadavers to verify the presence of the suprahoffatic recess. The infrahoffatic recess was present in 45% of the knees and mostly linear in shape (44%). The suprahoffatic recess was detected in 71% of the knees (45% in cadavers). Very weak to moderate positive correlation was found between the synovial effusion or the condition of the ACL and the presence and dimensions of the recesses. An awareness of the recesses in the infrapatellar fat pad is important in order to distinguish between pathology and anatomic variants on routine MR imaging of the knee.

Change of quadriceps angle values with age and activity.

OBJECTIVE: The lines connecting the anterior superior iliac spine to the center of the patella and the center of the patella to the tibial tuberosity make the quadriceps angle (Q angle), and this can be used as data for patellar alignment. We undertook this study to provide detailed information about the change of Q angle values with age and activity. METHODS: The study was conducted on 474 active (AG) (soccer players) and 765 sedentary (SG) boys (N=1239) from the age of 9-19, and the sedentary group also served as control to their age matched active counterparts. The statistical methods used were the student's t-test and the 3 way analysis of variance (ANOVA). The study was carried out in the laboratories of the Anatomy Departments and School of Physical Education and Sports, Istanbul and Hacettepe Universities between 2001 and 2003. RESULTS: The right and left Q angle values within both groups were statistically insignificant. The comparison of the groups showed a very high level of significant difference between the groups for both knees (AG right Q angle = 14.54 +/- 4.76, SG right Q angle = 17.98 +/- 3.24; AG left Q angle = 14.41 +/- 4.61, SG left Q angle = 18.12 +/- 3.55). The 3 way ANOVA showed that the age and physical activity had equally highly significant effects on Q angle values with a greater change in the active group's values. CONCLUSION: We conclude that 1) children and adolescents have greater Q angle values than adults, 2) a change in quadriceps strength and tone, caused by both growth and activity, results in a decrease of the Q angle and 3) activity, particularly playing soccer in our study, has a remarkable effect on the Q angle.

Vascular pedicles of infrahyoid muscles: an anatomical study.

This study was designed to determine the entering sites of vascular pedicles of the infrahyoid muscles. The neck regions of 12 cadavers were investigated bilaterally. The vascular pedicles of the infrahyoid muscles (except the thyrohyoid and inferior belly of the omohyoid muscle) were dissected and measurements taken with a caliper in reference to the attachments of the muscles. For the sternothyroid muscle, two vascular pedicles were found bilaterally in all cadavers. Two vascular pedicles were found bilaterally for the sternohyoid muscle in 11 cadavers; in one cadaver the inferior vascular pedicle was absent bilaterally. One vascular pedicle was found bilaterally for the superior belly of the omohyoid muscle in all cadavers. These data regarding the vascular pedicles of the infrahyoid muscles might be useful for preserving the vascular supply of the infrahyoid muscles during flap preparation.

The significance of the neurovascular structures passing through the spinoglenoid notch.

OBJECTIVE: To define the detailed anatomy of the neurovascular bundle at the spinoglenoid notch and to report the dimensions of these structures in cadavers. METHODS: In the present study, the external diameters of suprascapular artery, vein and nerve were measured at the spinoglenoid notch region in 18 formalin fixed cadavers (36 shoulders) by using a caliper. The study was carried out in the dissection laboratory of Anatomy Departments of Hacettepe University, Ankara University, Ankara and Mersin University, Mersin, Turkey, between 2002 and 2003. RESULTS: The average external diameter for the suprascapular vein was 2.6 mm, artery was 2.2 mm and nerve was 2.2 mm. The spinoglenoid notch was roofed by the spinoglenoid ligament and appeared as a fibroosseous foramen in all cadavers. We found that the vascular structures (suprascapular artery and vein) occupied 68.5% and the suprascapular nerve occupied 31.5% of this foramen. CONCLUSION: Although the diameters of the vascular structures at the spinoglenoid notch measured by magnetic resonance imaging have been reported, to our knowledge, external diameters of these structures at the spinoglenoid notch have not been described in cadavers. We believe that detailed anatomy of suprascapular neurovascular bundle at the spinoglenoid notch should be appreciated for better understanding of risk factors possibly causing the suprascapular nerve entrapment syndrome, specially for those who are involved in violent overhead sports activities such as volleyball and baseball.

Three-dimensional structure of the modiolus. A computerized reconstruction study.

OBJECTIVE: Modiolus is a dense, compact, mobile fibromuscular structure, reachable by the dissection of buccolabial musculature. We undertook this study to provide detailed information about the macroscopic and microscopic anatomy of modiolus and its 3-dimensional (3-D) shape. METHODS: Fifteen micrometer thick serial sections were taken from the fixed cadaveric tissue samples and then stained with hematoxylin and eosin, and Massons' trichrome stain. Stained sections were photographed digitally and images were transferred to computer medium to perform 3-D reconstruction. The study was carried out in the dissection lab of the Anatomy Department of Hacettepe University, Ankara, Turkey between 2002 and 2003. RESULTS: The modiolus appears to be a tortuous, blunt conelike structure, extending vertically from the buccal mucosa to the dermis of the skin. The base of the cone is adjacent to the mucosa. CONCLUSION: As the structure has great clinical importance, and satisfactory complete 3-D analysis of the subject is not yet available; we believe that our study presenting the histological and 3-D description of the modiolus will be of great help to surgeons for satisfactory esthetic and functional results during surgical operations related to the oral commissure.

Bilateral additional renal arteries and an additional right renal vein associated with unrotated kidneys.

We report a rare anomaly of the kidneys and its vessels, which were found during the routine dissection of a 68-year-old male cadaver. The anomaly consisted of bilateral additional renal arteries originating from the abdominal aorta and an additional right renal vein accompanying the additional right renal artery. These anomalies were associated with unrotated kidneys with extrarenal calices and pelves. All the additional vessels were located posterior to the ureter with a close relationship to the ureteropelvic junction on the right side. Additional renal vessels arise as a result of the complicated development of kidneys and variations in the positional anatomy of the kidneys, and their vascular supply are of clinical importance.