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1.
Int J Parasitol ; 41(11): 1139-47, 2011 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-21802422

RESUMEN

Little is known of the genetic diversity of Toxoplasma gondii circulating in wildlife. In the present study wild animals, from the USA were examined for T. gondii infection. Tissues of naturally exposed animals were bioassayed in mice for isolation of viable parasites. Viable T. gondii was isolated from 31 animals including, to our knowledge for the first time, from a bald eagle (Haliaeetus leucocephalus), five gray wolves (Canis lupus), a woodrat (Neotoma micropus), and five Arctic foxes (Alopex lagopus). Additionally, 66 T. gondii isolates obtained previously, but not genetically characterised, were revived in mice. Toxoplasma gondii DNA isolated from these 97 samples (31+66) was characterised using 11 PCR-restriction fragment length polymorphism (RFLP) markers (SAG1, 5'- and 3'-SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico). A total of 95 isolates were successfully genotyped. In addition to clonal Types II, and III, 12 different genotypes were found. These genotype data were combined with 74 T. gondii isolates previously characterised from wildlife from North America and a composite data set of 169 isolates comprised 22 genotypes, including clonal Types II, III and 20 atypical genotypes. Phylogenetic network analysis showed limited diversity with dominance of a recently designated fourth clonal type (Type 12) in North America, followed by the Type II and III lineages. These three major lineages together accounted for 85% of strains in North America. The Type 12 lineage includes previously identified Type A and X strains from sea otters. This study revealed that the Type 12 lineage accounts for 46.7% (79/169) of isolates and is dominant in wildlife of North America. No clonal Type I strain was identified among these wildlife isolates. These results suggest that T. gondii strains in wildlife from North America have limited diversity, with the occurrence of only a few major clonal types.


Asunto(s)
Toxoplasma/genética , Toxoplasma/aislamiento & purificación , Toxoplasmosis Animal/epidemiología , Toxoplasmosis Animal/parasitología , Animales , Animales Domésticos/parasitología , Animales Salvajes/parasitología , Gatos , Variación Genética , Genotipo , Ratones , Datos de Secuencia Molecular , América del Norte , Filogenia , Prevalencia , Roedores , Porcinos , Toxoplasma/clasificación
2.
J Wildl Dis ; 45(2): 505-7, 2009 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-19395761

RESUMEN

Subcutaneous dermoid cysts were identified in eight wild caribou (Rangifer tarandus) from widely dispersed locations in northern Canada and in one wild caribou from Alaska. The dermoid cysts from Canadian caribou were found among 557 diagnostic specimens that had been detected by hunters and submitted by resource officers and biologists between 1 January 1966 and 15 May 2007. All of the cysts were located in the cervical region, and five of nine cysts were found in the throat area. All of the animals for which the age was known were adult; males and females were equally represented. Dermoid cysts were not diagnosed in any of 1,108 white-tailed deer (Odocoileus virginianus), 293 mule deer (Odocoileus hemionus), 174 elk (Cervus elaphus), or 529 moose (Alces alces) examined during the same period at the Canadian laboratory.


Asunto(s)
Quiste Dermoide/veterinaria , Reno , Neoplasias Cutáneas/veterinaria , Alaska/epidemiología , Animales , Animales Salvajes , Canadá/epidemiología , Quiste Dermoide/epidemiología , Femenino , Masculino , Cuello/patología , Faringe/patología , Vigilancia de Guardia/veterinaria , Neoplasias Cutáneas/epidemiología
3.
Arch Virol ; 151(3): 423-38, 2006 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-16328132

RESUMEN

Novel poxviruses were identified in skin lesions of several species of cetaceans and pinnipeds using polymerase chain reaction targeting DNA polymerase and DNA topoisomerase I genes of members of the subfamily Chordopoxvirinae. With the exception of parapoxviruses, no molecular data of marine mammal poxviruses were available to infer genetic and evolutionary relatedness to terrestrial vertebrate poxviruses. Viruses were assigned to a cetacean poxvirus 1 (CPV-1) group based on nucleotide and amino acid identities of gene fragments amplified from skin lesions of Asian bottlenose (Tursiops aduncus), Atlantic bottlenose (Tursiops truncatus), rough-toothed (Steno bredanensis), and striped (Stenella coeruleoalba) dolphins. A different poxvirus was detected in skin lesions of a bowhead whale (Balaena mysticetus) and provisionally assigned to a CPV-2 group. These viruses showed highest identity to terrestrial poxviruses of the genera Orthopoxvirus and Suipoxvirus. A novel species-specific poxvirus was also identified in skin lesions of Steller sea lions (Eumetopias jubatus). None of these poxviruses were found to have amplifiable hemagglutinin gene sequences. Novel parapoxviruses were also identified in skin lesions of Steller sea lions and spotted seals (Phoca largha). A significant degree of divergence was observed in sequences of Steller sea lion parapoxviruses, while those of spotted seals and harbor seals (Phoca vitulina) were highly conserved.


Asunto(s)
Caniformia/virología , Cetáceos/virología , Poxviridae/genética , Poxviridae/aislamiento & purificación , Alaska , Animales , Secuencia de Bases , Chordopoxvirinae/clasificación , Chordopoxvirinae/genética , Chordopoxvirinae/aislamiento & purificación , ADN-Topoisomerasas de Tipo I/genética , ADN Viral/genética , ADN Polimerasa Dirigida por ADN/genética , Genes Virales , Genes env , Hemaglutininas Virales/genética , Biología Marina , Filogenia , Reacción en Cadena de la Polimerasa , Poxviridae/clasificación
4.
Sci Total Environ ; 231(2-3): 183-200, 1999 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-10472134

RESUMEN

Levels of organochlorine contaminants in blood of northern fur seal (Callorhinus ursinus) pups and the blood and milk of their dams early in the lactation period are reported here. The contaminants included 15 selected individual polychlorinated biphenyl (PCB) congeners and DDT metabolites identified through high-performance liquid chromatography. Congeners CB-77, -81, -126, -169 and -189 were below the limits of detection in milk and blood samples analyzed. Congener-specific concentrations of PCBs in the blood of pups were compared based on the age of their dam (< or = 5 years or > 7 years). Pups of young (presumably primiparous) dams had significantly elevated levels of CBs-101, -118, -128, -138, -153/87, -170/194, and -180 than pups of older (multiparous) dams. Congeners CB-128 and -170/194 were detected in the blood of pups of young dams but not in the blood of pups of older dams nor in any of the dams blood. Additionally, pups had higher blood levels in seven of 10 detected PCB congeners as compared to the levels measured in milk when adjusted for lipid content. Levels of DDT metabolites and toxic equivalency quotients of dioxin-like congeners followed similar trends. Lipid-normalized concentrations of CB-101 and total PCBs were significantly higher in the blood of dams than in their milk. CB-128, -156, -157, -170/194, p,p'-DDT, o,p'-DDT, p,p'-DDD and o,p'-DDD were not detected in dam blood samples, but were detected in milk samples. Calculation of 'biomagnification factors' from milk to pup blood indicated a biomagnification of CB-101, -105, -118, -138, -153/87, and -180. Significant mean accumulation factors ranged from 1.5 to 7.5. Inter-annual differences in exposure levels and specific congener concentrations in both milk and blood were apparent. Northern fur seal pups, especially first-born, have a substantial exposure to organochlorine contaminants at a critical developmental stage.


Asunto(s)
Contaminantes Ambientales/sangre , Contaminantes Ambientales/metabolismo , Lobos Marinos/sangre , Lobos Marinos/metabolismo , Hidrocarburos Clorados/sangre , Hidrocarburos Clorados/metabolismo , Leche/metabolismo , Factores de Edad , Alaska , Animales , Animales Recién Nacidos , DDT/sangre , DDT/metabolismo , Femenino , Masculino , Bifenilos Policlorados/sangre , Bifenilos Policlorados/metabolismo
5.
J Wildl Dis ; 33(3): 438-49, 1997 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-9249688

RESUMEN

From 1984 through 1992, staff at The Marine Mammal Center (TMMC, Sausalito, California, USA) examined 207 northern elephant seals (Mirounga angustirostris) with a condition of unknown etiology called northern elephant seal skin disease (NESSD). The skin lesions were characterized by patchy to extensive alopecia and hyperpigmentation, punctate or coalescing epidermal ulceration, and occasionally, massive skin necrosis. Microscopic lesions included ulcerative dermatitis with hyperkeratosis, squamous metaplasia and atrophy of sebaceous glands. All diseased seals were less than 2 years of age and suffered from emaciation, depression, and dehydration. Mortality from septicemia increased significantly with severity of skin ulceration. Compared to 14 apparently unaffected seals, diseased seals had depressed levels of circulating thyroxine, triiodothyronine, retinol, serum iron, albumin, calcium, and cholesterol. Levels of alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, gamma glutamyl transpeptidase, blood urea nitrogen, and uric acid were elevated. Morphometrically, diseased animals were approximately 15% smaller than normal seals of the same sage. Serum and blubber concentrations of 36 polychlorinated biphenyl congeners (sigma PCB) and dichloro-diphenyl-dichloroethylene (p,p'-DDE) were negatively correlated with body mass. Mean concentrations of sigma PCB and p,p'-DDE in serum in diseased seals were elevated as compared to apparently normal seals. Etiology of this syndrome remains unknown, but the possibility of PCB toxicosis cannot be ruled out.


Asunto(s)
Phocidae , Enfermedades de la Piel/veterinaria , Piel/patología , Alopecia/sangre , Alopecia/patología , Alopecia/veterinaria , Animales , Biopsia/veterinaria , California , Estudios de Cohortes , Femenino , Masculino , Estudios Prospectivos , Estudios Retrospectivos , Glándulas Sebáceas/patología , Piel/microbiología , Enfermedades de la Piel/sangre , Enfermedades de la Piel/patología , Úlcera Cutánea/patología , Úlcera Cutánea/veterinaria
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