Stable population structure in Europe since the Iron Age, despite high mobility.

Ancient DNA research in the past decade has revealed that European population structure changed dramatically in the prehistoric period (14,000-3000 years before present, YBP), reflecting the widespread introduction of Neolithic farmer and Bronze Age Steppe ancestries. However, little is known about how population structure changed from the historical period onward (3000 YBP - present). To address this, we collected whole genomes from 204 individuals from Europe and the Mediterranean, many of which are the first historical period genomes from their region (e.g. Armenia and France). We found that most regions show remarkable inter-individual heterogeneity. At least 7% of historical individuals carry ancestry uncommon in the region where they were sampled, some indicating cross-Mediterranean contacts. Despite this high level of mobility, overall population structure across western Eurasia is relatively stable through the historical period up to the present, mirroring geography. We show that, under standard population genetics models with local panmixia, the observed level of dispersal would lead to a collapse of population structure. Persistent population structure thus suggests a lower effective migration rate than indicated by the observed dispersal. We hypothesize that this phenomenon can be explained by extensive transient dispersal arising from drastically improved transportation networks and the Roman Empire's mobilization of people for trade, labor, and military. This work highlights the utility of ancient DNA in elucidating finer scale human population dynamics in recent history.

A genetic history of the Balkans from Roman frontier to Slavic migrations.

The rise and fall of the Roman Empire was a socio-political process with enormous ramifications for human history. The Middle Danube was a crucial frontier and a crossroads for population and cultural movement. Here, we present genome-wide data from 136 Balkan individuals dated to the 1st millennium CE. Despite extensive militarization and cultural influence, we find little ancestry contribution from peoples of Italic descent. However, we trace a large-scale influx of people of Anatolian ancestry during the Imperial period. Between ∼250 and 550 CE, we detect migrants with ancestry from Central/Northern Europe and the Steppe, confirming that "barbarian" migrations were propelled by ethnically diverse confederations. Following the end of Roman control, we detect the large-scale arrival of individuals who were genetically similar to modern Eastern European Slavic-speaking populations, who contributed 30%-60% of the ancestry of Balkan people, representing one of the largest permanent demographic changes anywhere in Europe during the Migration Period.

A case of hydrocephalus in a child from early modern period Zumberak, Croatia.

OBJECTIVE: To evaluate the etiology of a subadult skeleton exhibiting increased size of the neurocranium and upper face. MATERIALS: Skeletal remains of a child aged between 1.5-2 years from the Early Modern Period (14th -18th), Zumberak, Croatia. METHODS: Metric and volumetric data were collected, and CT with VRT reconstruction was employed. RESULTS: Metric analyses indicate that the neurocranium and upper face display increased size and volume. CONCLUSION: A diagnosis of the communicating form of hydrocephalus is suggested. SIGNIFICANCE: This is the first published case of the communicating form of hydrocephalus. LIMITATIONS: It is difficult to determine the specific etiology of this condition based on skeletal remains.

Sex determination by discriminant function analysis of the tibia for contemporary Croats.

Previous studies have demonstrated that populations differ from each other in size and proportion, and that these differences can affect metric assessment of sex. This paper establishes standards for determining sex from fragmentary and complete tibiae in the modern Croatian population. Measurements were taken on 180 tibiae (109 male and 71 female) from positively identified victims of the 1991-1995 War in Croatia. Six standard dimensions: length of the tibia (CML), maximum epiphyseal breadth of the proximal tibia (MPEB), maximum epiphyseal breadth of the distal tibia (MDEB), maximum diameter of the tibia at the nutrient foramen (MDNF), transverse diameter of the tibia at the nutrient foramen (TDNF), and circumference of the tibia at the nutrient foramen (CNF), were taken and subjected to different discriminant function analyses. The highest level of accuracy (91.1%) in the analyzed data set was achieved employing the variables: maximum epiphyseal breadth of the proximal tibia, maximum epiphyseal breadth of the distal tibia, maximum diameter of the tibia at the nutrient foramen, transverse diameter of the tibia at the nutrient foramen, and circumference of the tibia at the nutrient foramen. The second highest level of accuracy (90.6%) was achieved using a combination of only three variables: maximum epiphyseal breadth of the proximal tibia, maximum diameter of the tibia at the nutrient foramen, and circumference of the tibia at the nutrient foramen. The lowest accuracy (84.4%) was obtained when only one variable (maximum diameter of the tibia at the nutrient foramen) was employed. The results of this study show that the modern Croatian tibia is a good skeletal component for determining sex. Standardized coefficients of the discriminant functions generated in this study support the results of previous studies that found that breadth dimensions provide better separation of the sexes than length.

Bone fractures as indicators of intentional violence in the eastern Adriatic from the antique to the late medieval period (2nd-16th century AD).

To test the historically documented hypothesis of a general increase in deliberate violence in the eastern Adriatic from the antique (AN; 2nd-6th c.) through the early medieval (EM; 7th-11th c.) to the late-medieval period (LM; 12th-16th c.), an analysis of the frequency and patterning of bone trauma was conducted in three skeletal series from these time periods. A total of 1,125 adult skeletons-346 from the AN, 313 from the EM, and 466 from the LM series-were analyzed. To differentiate between intentional violence and accidental injuries, data for trauma frequencies were collected for the complete skeleton, individual long bones, and the craniofacial region as well as by type of injury (perimortem vs. antemortem). The results of our analyses show a significant temporal increase in total fracture frequencies when calculated by skeleton as well as of individuals exhibiting one skeletal indicator of deliberate violence (sharp force lesions, craniofacial injuries, "parry" fractures, or perimortem trauma). No significant temporal increases were, however, noted in the frequencies of craniofacial trauma, "parry" fractures, perimortem injuries, or of individuals exhibiting multiple skeletal indicators of intentional violence. Cumulatively, these data suggest that the temporal increase in total fracture frequencies recorded in the eastern Adriatic was caused by a combination of factors that included not only an increase of intentional violence but also a significant change in lifestyle that accompanied the transition from a relatively affluent AN urban lifestyle to a more primitive rural medieval way of life.

The harsh life on the 15th century Croatia-Ottoman empire military border: analyzing and identifying the reasons for the massacre in Cepin.

Excavation of the historic period cemetery in Cepin, Croatia revealed the presence of a large number of perimortem injuries distributed among males, females, and subadults. Archaeological and historical data suggest these individuals were victims of a raid carried out by Turkish akinji light cavalry in 1441. Comparisons with the frequencies of perimortem trauma in 12 other, temporally congruent skeletal series from the Balkans (n = 2,123 skeletons) support this assumption. The role of the akinji in the Ottoman army was twofold: to supply war captives, and to terrorize and disperse local populations before the advance of regular troops. This article tests the hypothesis that the purpose of the 1441 raid was the latter. To accomplish this, perimortem trauma in the series were analyzed by sex, age, location, and depth of the injury. A total of 82 perimortem injuries were recorded in 12 males, 7 females, and 3 subadults. The demographic profile of the victims suggests that young adults were specifically targeted in the attack. Significant sex differences are noted in the number, distribution, and pattern of perimortem trauma. Females exhibit significantly more perimortem injuries per individual, and per bone affected, than males. The morphology and pattern of perimortem trauma in females is suggestive of gratuitous violence. Cumulatively, analysis of the osteological data suggest that the objective of the 1441 akinji raid was to spread terror and panic in the Cepin area, either as revenge for recent military setbacks, or as part of a long-term strategy intended to depopulate the area around Osijek.