RESUMEN
A massive fish mortality of the major species, viz., Mugil cephalus, Chanos chanos, and Oreochromis mossambicus, occurred on November 27, 2017 in the Adyar estuary. This catastrophe followed a spell of heavy rainfall and flash floods. A detailed study of water quality parameters (pH, water temperature, salinity, total suspended matter, dissolved oxygen, biochemical oxygen demand, and nutrients) and heavy metals, i.e., chromium (Cr), copper (Cu), cadmium (Cd), nickel (Ni), lead (Pb), and zinc (Zn), in the sediment and fish tissues were analyzed. Pollution indices like the enrichment factor (EF), geo-accumulation index (Igeo), contamination factor (CF), pollution load index (PLI), potential ecological risk index (PERI), and biota sediment accumulation factor (BSAF) were used to measure heavy metals. The pollution indices revealed that metals were significantly enriched in the sediments. The Igeo indicates that there was moderate contamination by Cd (2.27-3.25), whereas CF shows high contamination by Cd (7.22-9.72) and moderate contamination by Pb (2.5-3.25). The PLI (1.04-1.13) suggests that heavy metal contamination of sediments has occurred. Results showed that high concentrations of the toxic metals Pb and Cd were found in the sediment as well as in fish tissues. Length and weight of fish are significantly correlated (r2 = 0.98, p < 0.05). The study revealed that the mass fish kill was due to impulsive changes in the water quality and heavy metal pollution from untreated urban sewage discharges in this region.
Asunto(s)
Monitoreo del Ambiente , Estuarios , Peces , Metales Pesados , Contaminantes Químicos del Agua , Animales , Metales Pesados/análisis , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidad , India , Sedimentos Geológicos/química , BahíasRESUMEN
The present study investigates the abundance, distribution, and characterization (shape, size, colour, chemical composition) of microplastics (MPs) in surface water and sediment from the shelf region of the central east coast of India. The surface water and sediment samples were collected at varying depths (12.8-63 m) from 21 locations covering â¼1200 km. The mean abundance of MPs in surface water and sediments were 5.3 × 104 particles. km-2, 209 ± 99 particles. kg-1 of dry weight, respectively. Stereomicroscopy, Raman spectroscopy, and micro Fourier Transform Infra-red Spectroscopy (FTIR) were employed for the quantification and characterization of the polymers. Polyolefin (polyethylene and polypropylene) were the dominant polymers in both surface water and sediments indicating their source primarily land based. Surface water and sediment MPs were mostly blue coloured. Fibre (77%) and fragment (38%) were the dominant morphotypes in surface water and sediments, respectively. Surface characteristics studies using Scanning Electron Microscope (SEM) highlight the breakdown progress of the particles; Small MPs (<1 mm) account for >50% of the whole and dominant in the offshore region (10 km). The results reveal that the primary sources of MPs are most likely to be originating from riverine fluxes and fishing-based activities.
Asunto(s)
Microplásticos , Contaminantes Químicos del Agua , Bahías , Monitoreo del Ambiente/métodos , Sedimentos Geológicos/química , Plásticos/química , Agua , Contaminantes Químicos del Agua/análisisRESUMEN
Coastal water quality assessment is important to maintain a healthy environment for various uses including fisheries and recreation. Microbial populations are used as biological indicators of contamination to monitor water quality and are considered by the government to be one of the critical features for issuing safety guidelines. Different bacterial groups (pathogenic, vibrio and faecal) from five major recreational beaches of Chennai, India, were monitored for the assessment of coastal water quality. Faecal coliforms (FC) were high at all the beaches, with up to 4.2 × 105 CFU/mL and exceeding the normal standard limits of 100 CFU/100 mL set by the Central Pollution Control Board (CPCB) of India. Rainfall was found to have a role in the variability and distribution of indicator and pathogenic bacteria. The seasonal dry period witnessed elevated FC, while dilution in the wet period reduced Escherichia coli-like organisms (ECLO). High microbial counts were detected near the beach situated close to the river mouth, mainly due to discharges of untreated domestic sewage and industrial wastes. Similarly, the biological oxygen demand (BOD) was also high, 0.32 to 10.32 mg/L. Dissolved inorganic nitrogen (DIN) ranged from 2.21 to 134.53 µmol/L and inorganic phosphate (IP) ranged from 0 to 57.53 µmol/L. These values indicated the presence of significant untreated sewage in the coastal water. This study revealed that Chennai coastal waters carry high levels of faecal and pathogenic bacteria, detrimental for recreational and other contact activities. The quantitative and qualitative analyses will be useful for modelling and prediction of coastal water quality and management of other recreational beaches in India.
Asunto(s)
Aguas del Alcantarillado , Microbiología del Agua , Bacterias , Playas , Monitoreo del Ambiente , Heces , IndiaRESUMEN
Chidiyatappu Bay is one of the least disturbed marine environments of Andaman & Nicobar Islands, the union territory of India. Oceanic flushing from southeast and northwest direction is prevalent in this bay. Further, anthropogenic activity is minimal in the adjoining environment. Considering the pristine nature of this bay, seawater samples collected from 12 sampling stations covering three seasons were analyzed. Principal Component Analysis (PCA) revealed 69.9% of total variance and exhibited strong factor loading for nitrite, chlorophyll a and phaeophytin. In addition, analysis of variance (ANOVA-one way), regression analysis, box-whisker plots and Geographical Information System based hot spot analysis further simplified and supported multivariate results. The results obtained are important to establish reference conditions for comparative study with other similar ecosystems in the region.
Asunto(s)
Monitoreo del Ambiente/métodos , Análisis Multivariante , Agua de Mar/química , Análisis de Varianza , Bahías , Clorofila/análisis , Clorofila A , Ecosistema , Humanos , India , Islas , Nitritos/análisis , Océanos y Mares , Análisis de Componente Principal , SalinidadRESUMEN
Blooming of diatom species Chaetoceros curvisetus (Cleve, 1889) was observed in Junglighat Bay and Haddo Harbour of Port Blair Bay of Andaman and Nicobar Islands during June 2010. Physico-chemical parameters, nutrient concentrations and phytoplankton composition data collected from five stations during 2010 were classified as bloom area (BA) and non-bloom area (NBA) and compared. Elevated values of dissolved oxygen were recorded in the BA, and it significantly varied (p < 0.01) from NBA. Among the nutrient parameters studied, nitrate concentration indicated significant variation in BA and NBA (p < 0.01). Phosphate and ammonia concentrations reduced to below detection level (BDL) in the BA and NBA, indicating its utilization. In Junglighat Bay, the C. curvisetus species constituted 93.4 and 69.2% composition of total phytoplankton population during day 1 and day 2, respectively. The bloom forming stations separated out from the non-bloom forming station in non-parametric multidimensional scaling (nMDS) ordinations; cluster analysis powered by SIMPROF test also grouped the stations as BA and NBA.
Asunto(s)
Diatomeas/crecimiento & desarrollo , Fitoplancton/crecimiento & desarrollo , Contaminación del Agua/estadística & datos numéricos , Calidad del Agua , Bahías , Análisis por Conglomerados , Monitoreo del Ambiente , Islas , Nitratos/análisis , Contaminación del Agua/análisisRESUMEN
Studies were carried out for the optimization and production of novel extracellular glutaminase-free L-asparaginase from Nocardiopsis alba NIOT-VKMA08. Among the tested carbon and nitrogen sources, maximum L-asparaginase production was observed with a combination of L-asparagine and maltose (1.5%) and twofold increase in yield (18.47 IU mL(-1)) was observed with newly optimized NIOT-asparaginase medium. Activity of the purified enzyme was moderately inhibited by various divalent cations and thiol group blocking reagents, with K(m) and V(max) of 0.127 mM and 5.50 U µg(-1). Optimum pH and temperature of purified L-asparaginase for the hydrolysis of L-asparagine was 8.0 and 37 °C, respectively. The enzyme inhibited polyacrylamide formation in 10% solution and it was very specific for its natural substrate L-asparagine. Partial glutaminase activity was not detected, which could reduce the possibility of side effects during cancer therapy. L-Asparaginase biosynthesis gene (ansA) was cloned and transformed in E. coli JM109. The ansA gene sequence reported in this study contains several base substitutions with that of reported sequences in GenBank, resulting in altered amino acid sequences of the translated protein.
Asunto(s)
Actinobacteria/clasificación , Actinobacteria/enzimología , Asparaginasa/biosíntesis , Asparaginasa/química , Glutaminasa/química , Secuencia de Aminoácidos , Asparaginasa/aislamiento & purificación , Activación Enzimática , Estabilidad de Enzimas , Datos de Secuencia Molecular , Especificidad de la Especie , Especificidad por SustratoRESUMEN
Aerial Bay is one of the harbor towns of Andaman and Nicobar Islands, the union territory of India. Nevertheless, it is least studied marine environment, particularly for physico-chemical assessment. Therefore, to evaluate the annual spatiotemporal variations of physico-chemical parameters, seawater samples collected from 20 sampling stations covering three seasons were analyzed. Multivariate statistics is applied to the investigated data in an attempt to understand the causes of variation in physico-chemical parameters. Cluster analysis distinguished mangrove and open sea stations from other areas by considering distinctive physico-chemical characteristics. Factor analysis revealed 79.5% of total variance in physico-chemical parameters. Strong loading included transparency, TSS, DO, BOD, salinity, nitrate, nitrite, inorganic phosphate, total phosphorus and silicate. In addition, box-whisker plots and Geographical Information System based land use data further facilitated and supported multivariate results.
Asunto(s)
Monitoreo del Ambiente/métodos , Nitratos/análisis , Agua de Mar/análisis , Análisis de Varianza , Bahías , Análisis por Conglomerados , Análisis Factorial , India , Análisis Multivariante , Nitritos/análisis , Océanos y Mares , Fosfatos/análisis , Fósforo/análisis , Análisis de Componente Principal , Salinidad , Estaciones del Año , Silicatos/análisisRESUMEN
Port Blair is the capital city of Andaman & Nicobar Islands, the union territory of India. More than 50% of the population of these islands lives around Port Blair Bay. Therefore the anthropogenic effects in the bay water were studied for monitoring purpose from seven stations. Physico-chemical parameters of seawater were analyzed in samples collected once in every 3 months for 2 years from seven sampling stations located in Port Blair Bay, South Andaman Island to evaluate the spatial and tidal variation. Cluster analysis and factor analysis were applied to the experimental data in an attempt to understand the sources of variation of physico-chemical parameters. In cluster analysis, the stations Junglighat Bay and Phoenix Bay having high anthropogenic influence formed a separate group. The factors obtained from factor analysis indicated that the parameters responsible for physico-chemical variations are mainly related to land run-off, sewage outfall and tidal flow.