RESUMEN
The dynamics of lung microbiota in tuberculosis remains poorly understood. Sequencing of variable regions of the 16S rRNA gene from surgically excised tuberculosis foci and biopsy specimens of normal lung tissue allowed characterization of the diversity and predictive potential of bacterial communities. Taxonomic diversity indices attested to differences in the structure of microbial communities between "healthy" lungs and tuberculomas. The microbial composition of "healthy" lungs varied in taxonomic diversity and was presented by both gram-positive and gram-negative bacteria with sufficiently similar metabolic potential. The microbiota of the examined tuberculomas consisted of Mycobacterium tuberculosis in 99.9% of cases. A significant part of the metabolic pathways predicted by PICRUSt2 included cholesterol catabolism, sulfate assimilation, and various pathways for the biosynthesis of cell wall components.
Asunto(s)
Pulmón , Mycobacterium tuberculosis , ARN Ribosómico 16S , Tuberculoma , Humanos , ARN Ribosómico 16S/genética , Mycobacterium tuberculosis/genética , Tuberculoma/microbiología , Tuberculoma/patología , Tuberculoma/genética , Pulmón/microbiología , Pulmón/patología , Pulmón/metabolismo , Microbiota/genética , Microbiota/fisiología , Masculino , Adulto , Tuberculosis Pulmonar/microbiología , Femenino , Persona de Mediana Edad , Bacterias Gramnegativas/genética , Bacterias Grampositivas/genética , Bacterias Grampositivas/metabolismo , Bacterias Grampositivas/clasificaciónRESUMEN
We compared alpha diversity indices of the intestinal microbiota in adolescents with obesity and normal body weight, taking into account their ethnicity. Intestinal biocenosis was studied by metasequencing of amplicon libraries of V3-V4 fragments of the 16S rRNA gene. The alpha diversity of the microbiota was assessed using classical and alternative indices. Statistically significant differences in intestinal microbiota were observed between Russians with obesity and Buryats with normal body weight, as well as between Russians with obesity and Buryats with obesity when assessing the Shannon-Weaver, Chao1 indices, Faith phylogenetic diversity index, ACE, Fisher, Gini coefficient, Margalef, and Menkhinik indices. It was shown that alpha diversity indices can be used to assess significance of differences and variability of the intestinal microbiota in multifactorial diseases such as obesity in adolescents; however, the scope of application of the criteria should be considered.
