Circulating markers of intestinal barrier injury and inflammation following exertion in hypobaric hypoxia.

Hypoxia induced intestinal barrier injury, microbial translocation, and local/systemic inflammation may contribute to high-altitude associated gastrointestinal complications or symptoms of acute mountain sickness (AMS). Therefore, we tested the hypothesis that six-hours of hypobaric hypoxia increases circulating markers of intestinal barrier injury and inflammation. A secondary aim was to determine if the changes in these markers were different between those with and without AMS. Thirteen participants were exposed to six hours of hypobaric hypoxia, simulating an altitude of 4572 m. Participants completed two 30-minute bouts of exercise during the early hours of hypoxic exposure to mimic typical activity required by those at high altitude. Pre- and post-exposure blood samples were assessed for circulating markers of intestinal barrier injury and inflammation. Data below are presented as mean ± standard deviation or median [interquartile range]. Intestinal fatty acid binding protein (Δ251 [103-410] pg•mL-1; p = 0.002, d = 0.32), lipopolysaccharide binding protein (Δ2 ± 2.4 µg•mL-1; p = 0.011; d = 0.48), tumor necrosis factor-α (Δ10.2 [3-42.2] pg•mL-1; p = 0.005; d = 0.25), interleukin-1ß (Δ1.5 [0-6.7] pg•mL-1 p = 0.042; d = 0.18), and interleukin-1 receptor agonist (Δ3.4 [0.4-5.2] pg•mL-1p = 0.002; d = 0.23) increased from pre- to post-hypoxia. Six of the 13 participants developed AMS; however, the pre- to post-hypoxia changes for each marker were not different between those with and without AMS (p > 0.05 for all indices). These data provide evidence that high altitude exposures can lead to intestinal barrier injury, which may be an important consideration for mountaineers, military personnel, wildland firefighters, and athletes who travel to high altitudes to perform physical work or exercise.

Could Orthostatic Stress Responses Predict Acute Mountain Sickness Susceptibility Prior to High Altitude Travel? A Pilot Study.

Bellovary, Bryanne N., Andrew D. Wells, Zachary J. Fennel, Jeremy B. Ducharme, Jonathan M. Houck, Trevor J. Mayschak, Ann L. Gibson, Scott N. Drum, and Christine M. Mermier. Could orthostatic stress responses predict acute mountain sickness susceptibility before high altitude travel? A pilot study. High Alt Med Biol. 24:19-26, 2023. Purpose: This study assessed head-up tilt (HUT) responses in relation to acute mountain sickness (AMS)-susceptibility during hypoxic exposure. Materials and Methods: Fifteen participants completed three lab visits: (1) protocol familiarization and cycle maximal oxygen consumption (VO2max) test; (2) HUT test consisting of supine rest for 20 minutes followed by 70° tilting for ≤40 minutes; and (3) 6 hours of hypobaric hypoxic exposure (4,572 m) where participants performed two 30-minute cycling bouts separated by 1 hour at a 50% VO2max workload within the first 3 hours and rested when not exercising. During HUT, systolic blood pressure (SBP), diastolic blood pressure, heart rate (HR), and variability (blood pressure variability [BPV] and HR variability [HRV]) were measured continuously. The AMS scores were determined after 6 hours of exposure. Correlations determined relationships between HUT cardiovascular responses and AMS scores. Repeated-measures analysis of variance (ANOVA) assessed differences between those with and without AMS symptoms during HUT. Results: Higher AMS scores correlated with greater change in SBP variability (r = 0.52, p = 0.048) and blunted changes in HRV (root mean square of successive differences between normal heartbeats r = 0.81, p = 0.001, percentage of adjacent normal sinus intervals that differ by more than 50 milliseconds [pNN50] r = 0.87, p < 0.001) during HUT. A pNN50 interaction (p = 0.02) suggested elevated cardiac sympathetic activity at baseline and a blunted increase in cardiac sympathetic influence throughout HUT in those with AMS (pNN50 baseline: AMS = 26.2% ± 15.3%, no AMS = 51.0% ± 13.5%; first 3 minutes into HUT: AMS = 17.2% ± 19.1%, no AMS = 17.1% ± 10.9%; end of HUT: AMS = 6.2% ± 9.1%, no AMS 11.0% ± 10.0%). Conclusions: The results suggest autonomic responses via HUT differ in AMS-susceptible individuals. Changes in HRV and BPV during HUT may be a promising predictive measurement for AMS-susceptibility, but further research is needed for confirmation.

The physiological, perceptual, and thermoregulatory responses to facemask use during exercise.

The use of masks in public settings and when around people has been recommended to limit the spread of Coronavirus disease 2019 (COVID-19) by major public health agencies. Several different types of masks classified as either medical- or non-medical grade are commonly used among the public. However, concerns with difficulty breathing, re-breathing exhaled carbon dioxide, a decrease in arterial oxygen saturation, and a decrease in exercise performance have been raised regarding the use of mask during exercise. We review the current knowledge related to the effect of different masks during exercise on cardiorespiratory, metabolic, thermoregulatory, and perceptual responses. As such, the current literature seems to suggest that there are minimal changes to cardiovascular, metabolic, and no changes to thermoregulatory parameters with facemask use. However, differences in ventilatory parameters have been reported with submaximal and maximal intensity exercise to volitional fatigue. Literature on perceptual responses to exercise indicate an impact on ratings of perceived exertion, dyspnea, and overall discomfort dependent on mask use as well as exercise intensity. In conclusion, data from the current literature suggests a minimal impact on physiological, perceptual, and thermoregulatory responses dependent on the type of mask used during exercise.

Metabolic Profile of Reciprocal Supersets in Young, Recreationally Active Women and Men.

ABSTRACT: Realzola, RA, Mang, ZA, Millender, DJ, Beam, JR, Bellovary, BN, Wells, AD, Houck, JM, and Kravitz, L. Metabolic profile of reciprocal supersets in young, recreationally active females and males. J Strength Cond Res 36(10): 2709-2716, 2022-Reciprocal supersets (RSSs) are a time-efficient style of resistance exercise (RE) that consist of performing 2 consecutive exercises with opposing muscle groups while limiting rest times between them. Previous research in men indicates a RSS has an increased physiological response when compared with traditional RE (TRAD). No between-sex comparison of metabolic data for RSSs exists. The purpose of this study was to create a metabolic profile for RSSs in men and women. Eighteen resistance-trained individuals underwent 2 bouts of volume-load equated RE: RSS and TRAD. Reciprocal superset exercises were split into 3 clusters: (a) hexagonal bar deadlift superset with leg press, (b) chest press superset with seated row, and (c) overhead dumbbell press superset with latissimus dorsi pull-downs. The TRAD protocol, doing the same exercises, emulated hypertrophy emphasis training. Oxygen uptake (V̇ o2 ), heart rate (HR), blood lactate ([BLa]), rate of perceived exertion (RPE), and excess post-exercise oxygen consumption (EPOC) were measured. Aerobic and anaerobic energy expenditure were estimated using V̇ o2 and lactate, respectively. The level of significance set for this study was p ≤ 0.05. Regardless of sex, a RSS elicited significantly greater average V̇ o2 , HR, [BLa], RPE, and anaerobic and aerobic energy expenditures, and was completed in a shorter time compared with TRAD ( p ≤ 0.05). When compared with women, men had significantly greater EPOC, average [BLa], and anaerobic and aerobic energy expenditures during RSSs ( p ≤ 0.05). The average [BLa] and aerobic energy expenditure of the men were also significantly greater than the women during TRAD ( p ≤ 0.05). This study suggests that a RSS is a metabolically demanding RE session that may elicit increases in musculoskeletal, cardiorespiratory, and physiological adaptations while decreasing the duration of exercise.

New Multisite Bioelectrical Impedance Device Compared to Hydrostatic Weighing and Skinfold Body Fat Methods.

The purpose of this study was to compare the Skulpt Chisel™ to seven-site skinfold (SKF) and hydrostatic weighing (HW) body fat percentage (%BF) estimates. Twenty-six participants (aged 24 ± 4 years; BMI 23.1 ± 3.5 kg·m-2) were assessed. Significant differences in %BF estimates were found for all methodological pairings; p < 0.05. The SKF method underestimated %BF compared to HW (-2.52 ± 3.42 %BF). The Skulpt Chisel™ overestimated %BF compared to both HW (3.38 ± 6.10 %BF) and SKF (5.90 ± 5.26 %BF). Limits of agreement comparing HW to Skulpt Chisel™ indicated a difference between 95% confidence interval bounds (Upper bound: 5.84 %BF, Lower bound 0.92 %BF) and for HW to SKF (Upper bound: -1.14 %BF, Lower bound: -3.91 %BF). Regression analysis showed no significant bias for any methodological pairing; (p > 0.05). In conclusion, the Skulpt Chisel™ method should be used with caution when evaluating %BF of adults with similar demographics reported in this study.

Perceived demands and postexercise physical dysfunction in CrossFit® compared to an ACSM based training session.

BACKGROUND: CrossFit® is considered an intense and extreme conditioning program (ECP) that can cause overtraining and injury. Exertional Rhabdomyolysis (ER) - breakdown of muscle tissue - after ECP has been reported in CrossFit® and might be linked to comparatively high rates of subjectively perceived exertion levels. Therefore, the present study aimed at recording symptoms of postexercise physical dysfunction (e.g., excessive muscle soreness, shortness of breath) following CrossFit® and ratings of perceived exertion (RPE) during CrossFit® compared with training according to the American College of Sports Medicine (ACSM) guidelines. METHODS: A validated questionnaire was completed by 101 CrossFit® (age: 35±8 years; weight: 79±16 kg) and 56 ACSM (age: 35±10 years; weight: 75±27 kg) participants. RESULTS: CrossFit® and ACSM groups, respectively, reported significantly different RPE levels of 7.3±1.7 and 5.5±1.4 (P≤0.001) and amounts of hard days per week of 4.0±1.1 and 3.5±1.4 (P=0.04). The five most frequent and hardest ECP workouts of the day (WODs) were Fran (47), Murph (27), Fight Gone Bad (10), Helen (9) and Filthy 50 (9). Presence of severe post-exercise symptoms was notably higher in CrossFit® for excessive fatigue (42 vs. 8; P<0.001), muscle soreness (96 vs. 48; P=0.04), muscle swelling (19 vs. 4; P=0.048), shortness of breath (13 vs. 1; P=0.02), muscle pain to touch (31 vs. 4; P=0.001), and limited muscle movement during workout (37 vs. 9; P=0.007). CONCLUSIONS: CrossFit® leads to "very hard" perceived exertion causing detrimental post-exercise effects on muscle and ventilatory function in experienced athletes. Improved training progression with adequate recovery schedules are needed to prevent severe muscle injury, such as ER.