DNA-Gated Graphene Field-Effect Transistors for Specific Detection of Arsenic(III) in Rice.

As one of the most toxic forms of arsenic, inorganic As(III) is easy to accumulate in rice, leading to severe public health problems. Effective control of As(III) requires the development of fast analytical methods for its detection with high sensitivity and specificity. Toward this end, in this work, we report the fabrication of an As(III) electrochemical sensor based on a solution-gated graphene transistor (SGGT) platform with a novel sensing mechanism. The gold gate electrode of the SGGT was modified with DNA probes and then blocked with bovine serum albumin (BSA). The specific interaction between As(III) and gold disrupted the adsorption states of DNA probes, redistributing surface charges on the gate electrode, further leading to potential drop changes at the interfaces of the gate electrode and graphene active layer. This new mechanism based on DNA-charge-redistribution-induced SGGT current responses (denoted as "DNA-SGGT") was found to greatly improve the selectivity of the sensor: the response of DNA-SGGT to As(III) was effectively enhanced fourfold, while to other interfering cations, it was significantly reduced. The optimized sensor showed a detection limit as low as 5 nM with superior selectivity to As(III). The as-prepared DNA-SGGT-based sensor has also been successfully applied to the detection of As(III) in practical rice samples with a high recovery rate, showing great potential for heavy metal detection in many types of food samples.

Single-Atom Enzyme-Functionalized Solution-Gated Graphene Transistor for Real-Time Detection of Mercury Ion.

Mercury ion (Hg2+), a bioaccumulating and toxic heavy metal, can cause severe damages to the environment and human health. Therefore, development of high-performance Hg2+ sensors is highly desirable. Herein, we construct a uniform dodecahedral shaped N-doped carbon decorated by single Fe site enzyme (Fe-N-C SAE), which exhibits good performance for Hg2+ detection. The N atom on Fe-N-C SAE can specifically recognize Hg2+ through chelation between Hg2+ and N atom, while the catalytic site on the single-atom enzyme acts as a signal amplifier. The Fe-N-C SAE-functionalized solution-gated graphene transistor exhibits a dramatic improvement in the selectivity and sensitivity of the devices. The sensor can rapidly detect Hg2+ down to 1 nM within 2 s. Besides, a relatively good repeatability and reproducibility for the detection of Hg2+ have also been found in our sensor platform. Our findings expand the application of single-atom catalysts in the field of food safety and environmental monitoring.

Porous carbon supported nanoceria derived from one step in situ pyrolysis of Jerusalem artichoke stalk for functionalization of solution-gated graphene transistors for real-time detection of lactic acid from cancer cell metabolism.

Effective detection of biomarkers for tumor cells has been the focus of attention. In this work, we have successfully fabricated a highly sensitive sensor based on solution-gated graphene transistors (SGGT) for detecting lactic acid content accumulated in tumor cells through their glycolysis metabolism. The sensing mechanism of the lactic acid sensor is attributed to electrochemical catalysis of H2O2 produced by the oxidation of lactic acid by lactate oxidase near the gate electrode. The key component of the sensor is the functionalization of porous carbon loaded with ceria nanoparticles derived from a novel one step in situ pyrolysis of pretreated Jerusalem artichoke stalk, which significantly improved the sensor sensitivity, i.e. a detection limit as low as 300 nM and linear range from 3 µM to 300 µM. The optimized lactic acid sensor has successfully applied to the detection of lactic acid in practical cell culture samples with high credibility. The SGGT-based lactic acid biosensor shows great potential for the application in tumor microenvironment due to its superior biocompatibility and accuracy.