Membrane potentials, oxidative stress and the dispersal response of bacterial biofilms to 405 nm light.

The majority of chronic infections are caused by biofilms, which have higher levels of antibiotic resistance than planktonic growth. Violet-blue 405 nm light has recently emerged as a novel bactericide, but limited studies have been conducted on its effectiveness against biofilms. We found that in response to 405 nm light both Pseudomonas aeruginosa and Bacillus subtilis biofilms exhibited cell dispersal and membrane potential hyperpolarisations. The response to 405 nm light depended on the stage of biofilm growth. The use of reactive oxygen species scavengers reduced membrane hyperpolarisation and biofilm dispersal in response to 405 nm light. This is the first time that membrane potential hyperpolarisations have been linked with photooxidative stress in bacteria and with biofilm dispersal. These results provide a new insight into the role of membrane potentials in the bacterial stress response and could be used in the development of 405 nm light based biofilm treatments.

Spatial propagation of electrical signals in circular biofilms: A combined experimental and agent-based fire-diffuse-fire study.

Bacterial biofilms are a risk to human health, playing critical roles in persistent infections. Recent studies have observed electrical signaling in biofilms and thus biofilms represent a new class of active excitable matter in which cell division is the active process and the spiking of the individual bacterial cells is the excitable process. Electrophysiological models have predominantly been developed to describe eukaryotic systems, but we demonstrate their use in understanding bacterial biofilms. Our agent-based fire-diffuse-fire (ABFDF) model successfully simulates the propagation of both centrifugal (away from the center) and centripetal (toward the center) electrical signals through biofilms of Bacillus subtilis. Furthermore, the ABFDF model allows realistic spatial positioning of the bacteria in two dimensions to be included in the fire-diffuse-fire model and this is the crucial factor that improves agreement with experiments. The speed of propagation is not constant and depends on the radius of the propagating electrical wave front. Centripetal waves are observed to move faster than centrifugal waves, which is a curvature driven effect and is correctly captured by our simulations.