RESUMEN
Natural history museum collections hold extremely rare, extinct species often described from a single known specimen. On occasions, rediscoveries open new opportunities to understand selective forces acting on phenotypic traits. Recent rediscovery of few individuals of Bocourt´s Terrific Skink Phoboscincus bocourti, from a small and remote islet in New Caledonia allowed to genetically identify a species of land crab in its diet. To explore this further, we CT- and MRI-scanned the head of the holotype, the only preserved specimen dated to about 1870, segmented the adductor muscles of the jaw and bones, and estimated bite force through biomechanical models. These data were compared with those gathered for 332 specimens belonging to 44 other skink species. Thereafter we recorded the maximum force needed to generate mechanical failure of the exoskeleton of a crab specimen. The bite force is greater than the prey hardness, suggesting that predation on hard-shelled crabs may be an important driver of performance. The high bite force seems crucial to overcome low or seasonal variations in resource availability in these extreme insular environments. Phoboscincus bocourti appears to be an apex predator in a remote and harsh environment and the only skink known to predate on hard-shelled land crabs.
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Braquiuros , Lagartos , Animales , Fenómenos Biomecánicos , Fuerza de la Mordida , Dieta , Humanos , Maxilares/anatomía & histología , Conducta PredatoriaRESUMEN
The few works on audition in sharks and rays concern only adult specimens. We report the hearing abilities in the dogfish Scyliorhinus canicula at different stages, from embryos that still have their yolk sac inside their egg, to juveniles. Hearing development corresponds to an increase in the frequency range from 100-300 Hz in early pre-hatching stages to 100-600 Hz in juveniles. Modifications in hearing abilities correspond to the development of the brain, the increase of the volume of the membranous labyrinth, the growth of the sensory epithelium, and the development of stereocilia in addition to kinocilium before hatching. This work offers solid insights into the development of hearing abilities that usually can only be inferred from the anatomy of vertebrates or after birth/hatching. It shows also that shark can be sensitive to background noise during development.
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Encéfalo/fisiología , Cazón/fisiología , Oído Interno/fisiología , Audición/fisiología , Animales , Encéfalo/crecimiento & desarrollo , Oído Interno/crecimiento & desarrolloRESUMEN
We here present the first detailed study of the specimen KNM-RU 18340 from Rusinga Island (Kenya), the only known complete early Miocene chameleon skull, using micro-CT. This specimen represents one of the oldest chameleon fossils ever recovered. For the first time, the skull bone internal surfaces, their sutures, and elements contained inside the rocky matrix are observed. Our morphological comparisons and phylogenetic analyses place this specimen confidently in the genus Calumma and a new species, Calumma benovskyi sp. nov., is erected for it. Since all species of this genus are endemic to Madagascar, this fossil uniquely demonstrates the existence of Calumma on continental Africa in the past. Our results challenge the long-held view that chameleons originated on Madagascar and dispersed over water to Africa, and provide a strong evidence of an African origin for some Malagasy lineages. The Oligocene-early Miocene dispersal to Madagascar, using oceanic currents that favoured eastward dispersal at that time, is a highly supported scenario matching the suggested dispersal of lemurs to this island. This is consistent with a previously suggested hypothesis based on molecular data.
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Fósiles/anatomía & histología , Lagartos/anatomía & histología , Cráneo/anatomía & histología , África , Animales , Evolución Biológica , Fósiles/diagnóstico por imagen , Madagascar , Masculino , Microtomografía por Rayos XRESUMEN
Being implied in flight, mimetism, communication, and protection, the insect wings were crucial organs for the mega diversification of this clade. Despite several attempts, the problem of wing evolution remains unresolved because the basal parts of the veins essential for vein identification are hidden in the basivenal sclerites. The homologies between wing characters thus cannot be accurately verified, while they are of primary importance to solve long-standing problems, such as the monophyly of the Palaeoptera, viz. Odonatoptera, Panephemeroptera, and Palaeozoic Palaeodictyopterida mainly known by their wings. Hitherto the tools to homologize venation were suffering several cases of exceptions, rendering them unreliable. Here we reconstruct the odonatopteran venation using fossils and a new 3D imaging tool, resulting congruent with the concept of Riek and Kukalová-Peck, with important novelties, viz. median anterior vein fused to radius and radius posterior nearly as convex as radius anterior (putative synapomorphies of Odonatoptera); subcostal anterior (ScA) fused to costal vein and most basal primary antenodal crossvein being a modified posterior branch of ScA (putative synapomorphies of Palaeoptera). These findings may reveal critical for future analyses of the relationships between fossil and extant Palaeoptera, helping to solve the evolutionary history of the insects as a whole.
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Insectos/anatomía & histología , Modelos Anatómicos , Venas , Alas de Animales/irrigación sanguínea , Animales , Fósiles , Imagenología TridimensionalRESUMEN
Brain endocasts obtained from computed tomography (CT) are now widely used in the field of comparative neuroanatomy. They provide an overview of the morphology of the brain and associated tissues located in the cranial cavity. Through anatomical comparisons between species, insights on the senses, the behavior, and the lifestyle can be gained. Although there are many studies dealing with mammal and bird endocasts, those performed on the brain endocasts of squamates are comparatively rare, thus limiting our understanding of their morphological variability and interpretations. Here, we provide the first comparative study of snake brain endocasts in order to bring new information about the morphology of these structures. Additionally, we test if the snake brain endocast encompasses a phylogenetic and/or an ecological signal. For this purpose, the digital endocasts of 45 snake specimens, including a wide diversity in terms of phylogeny and ecology, were digitized using CT, and compared both qualitatively and quantitatively. Snake endocasts exhibit a great variability. The different methods performed from descriptive characters, linear measurements and the outline curves provided complementary information. All these methods have shown that the shape of the snake brain endocast contains, as in mammals and birds, a phylogenetic signal but also an ecological one. Although phylogenetically related taxa share several similarities between each other, the brain endocast morphology reflects some notable ecological trends: e.g. (i) fossorial species possess both reduced optic tectum and pituitary gland; (ii) both fossorial and marine species have cerebral hemispheres poorly developed laterally; (iii) cerebral hemispheres and optic tectum are more developed in arboreal and terrestrial species.
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Encéfalo/anatomía & histología , Serpientes/anatomía & histología , Anatomía Comparada , Animales , Filogenia , Tomografía Computarizada por Rayos XRESUMEN
When the same complex trait is exhibited by closely related species, a single evolutionary origin is frequently invoked. The complex stridulatory apparatus present in the forewings of extant crickets, mole crickets, katydids, and prophalangopsids, is currently interpreted as sharing a single common origin due to their similarity and unique function. An alternative hypothesis of convergent evolution in these ensiferan groups has challenged this common view, but remained controversial because of competing interpretations of wing venation. Here we propose another hypothesis for the widely and long debated homology of ensiferan stridulatory apparatus, performing the first 3D reconstruction of hidden structures at the wing bases. This approach allowed defining the homology of each vein from its very origin rather than after its more distal characteristics, which may be subjected to environmental pressure of selection. The stridulatory apparatus involves different veins in these four singing clades. In light of the most recent phylogenetic evidence, this apparatus developed four times in Ensifera, illustrating extraordinary convergent evolutions between closely related clades, by far exceeding the number of evolutionary steps ever proposed for calling ability in this group.
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Evolución Biológica , Imagenología Tridimensional , Insectos/anatomía & histología , Insectos/fisiología , Vocalización Animal , Animales , Insectos/clasificaciónRESUMEN
Today, Diploglossine lizards (Anguidae) are common on the Greater Antillean Islands (West Indies), where they are represented by many endemic species. However these lizards are very rare on the Lesser Antillean Islands, where they are only represented by a single species, the Montserrat galliwasp (Diploglossus montisserrati). Here, we show that diploglossine lizards were present in the past on other Lesser Antillean islands, by reporting the discovery of Anguidae fossil remains in two Amerindian archaeological deposits and in a modern deposit. These remains are compared to skeletons of extant diploglossine lizards, including D. montisserrati, using X-ray microtomography of the type specimen of this critically endangered lizard. We also conducted a histological study of the osteoderms in order to estimate the putative age of the specimen. Our results show that the fossil specimens correspond to a member of the Diploglossus genus presenting strong similarities, but also minor morphological differences with D. montisserrati, although we postulate that these differences are not sufficient to warrant the description of a new species. These specimens, identified as Diploglossus sp., provide a new comparison point for the study of fossil diploglossine lizards and reflect the historical 17(th) century mentions of anguid lizards, which had not been observed since.
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Fósiles , Lagartos/anatomía & histología , Lagartos/clasificación , Animales , Indias OccidentalesRESUMEN
The Hula Painted Frog (Latonia nigriventer) is a rare frog species endemic to the Hula Valley, Israel. The species is the sole relict of a clade that was widespread mainly in Europe from the Oligocene until the beginning of the Pleistocene. The osteological characteristics of L. nigriventer are described based on X-ray microtomography scans of extant specimens and Pleistocene bones from the Hula Valley, to elucidate the evolutionary history of Alytidae and more specifically of Latonia. Based on the osteological description of L. nigriventer, we now better understand the differences, between Latonia and its sister taxon Discoglossus. They differ mainly in their cranial structure with the reinforced skull of Latonia having powerful jaws. Latonia nigriventer can achieve great force while closing its jaws, due to increased adductor muscle insertion surfaces as expressed by the presence of an additional paracoronoid process and an enlarged upper margin of the postero-lateral wall of the lower jaw. In addition, a wider pterygoid fossa and higher canthus postero-lateralis of the frontoparietal, compared to that of Discoglossus, also suggest the presence of well-developed adductor muscles. Furthermore, L. nigriventer have particularly strong skulls as expressed by: long articulations between different skull elements, interdigitation in the contact area between the nasals and between nasals and the frontoparietals, and fused frontoparietals. Both males and females L. nigriventer have very robust forelimbs, as indicated by well-developed medial crests of the humerus. Based on limited eastern Mediterranean paleontological data, we can only suspect that the dispersal of Latonia into the Levant from Asia Minor occurred at some point during the Miocene or later. The first appearance of L. nigriventer in the Hula Valley, its current habitat, dates to approximately 780 thousand years ago at the archaeological site of Gesher Benot Ya'aqov. J. Morphol. 277:1131-1145, 2016. © 2016 Wiley Periodicals, Inc.
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Anuros/anatomía & histología , Evolución Biológica , Huesos/anatomía & histología , Animales , Arqueología , Ecosistema , Femenino , Fósiles/anatomía & histología , Israel , Masculino , Osteología , PaleontologíaRESUMEN
In this paper, ultrasound measurements of 1:1 scale three-dimensional (3D) printed trabecular bone phantoms are reported. The micro-structure of a trabecular horse bone sample was obtained via synchrotron x-ray microtomography, converted to a 3D binary data set, and successfully 3D-printed at scale 1:1. Ultrasound through-transmission experiments were also performed through a highly anisotropic version of this structure, obtained by elongating the digitized structure prior to 3D printing. As in real anisotropic trabecular bone, both the fast and slow waves were observed. This illustrates the potential of stereolithography and the relevance of such bone phantoms for the study of ultrasound propagation in bone.
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Epífisis/diagnóstico por imagen , Fémur/diagnóstico por imagen , Fantasmas de Imagen , Impresión Tridimensional , Ondas Ultrasónicas , Ultrasonografía/instrumentación , Animales , Diseño de Equipo , Caballos , Interpretación de Imagen Asistida por Computador , Movimiento (Física) , Programas Informáticos , Sincrotrones , Factores de Tiempo , Microtomografía por Rayos XRESUMEN
The bony labyrinth provides a proxy for the morphology of the inner ear, a primary cognitive organ involved in hearing, body perception in space, and balance in vertebrates. Bony labyrinth shape variations often are attributed to phylogenetic and ecological factors. Here we use three-dimensional (3D) geometric morphometrics to examine the phylogenetic and ecological patterns of variation in the bony labyrinth morphology of the most species-rich and ecologically diversified traditionally recognized superfamily of Carnivora, the Musteloidea (e.g. weasels, otters, badgers, red panda, skunks, raccoons, coatis). We scanned the basicrania of specimens belonging to 31 species using high-resolution X-ray computed micro-tomography (µCT) to virtually reconstruct 3D models of the bony labyrinths. Labyrinth morphology is captured by a set of six fixed landmarks on the vestibular and cochlear systems, and 120 sliding semilandmarks, slid at the center of the semicircular canals and the cochlea. We found that the morphology of this sensory structure is not significantly influenced by bony labyrinth size, in comparisons across all musteloids or in any of the individual traditionally recognized families (Mephitidae, Procyonidae, Mustelidae). PCA (principal components analysis) of shape data revealed that bony labyrinth morphology is clearly distinguishable between musteloid families, and permutation tests of the Kmult statistic confirmed that the bony labyrinth shows a phylogenetic signal in musteloids and in most mustelids. Both the vestibular and cochlear regions display morphological differences among the musteloids sampled, associated with the size and curvature of the semicircular canals, angles between canals, presence or absence of a secondary common crus, degree of lateral compression of the vestibule, orientation of the cochlea relative to the semicircular canals, proportions of the cochlea, and degree of curvature of its turns. We detected a significant ecological signal in the bony labyrinth shape of musteloids, differentiating semi-aquatic taxa from non-aquatic ones (the taxa assigned to terrestrial, arboreal, semi-arboreal, and semi-fossorial categories), and a significant signal for mustelids, differentiating the bony labyrinths of terrestrial, semi-arboreal, arboreal, semi-fossorial and semi-aquatic species from each other. Otters and minks are distinguished from non-aquatic musteloids by an oval rather than circular anterior canal, sinuous rather than straight lateral canal, and acute rather than straight angle between the posterior and lateral semicircular canals - each of these morphological characters has been related previously to animal sensitivity for detecting head motion in space.
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Oído Interno/anatomía & histología , Mephitidae/anatomía & histología , Mustelidae/anatomía & histología , Procyonidae/anatomía & histología , Puntos Anatómicos de Referencia , Animales , Evolución Biológica , Imagenología Tridimensional , Filogenia , Análisis de Componente Principal , Microtomografía por Rayos XRESUMEN
Palaeobatrachidae are extinct frogs from Europe closely related to the Gondwanan Pipidae, which includes Xenopus. Their frontoparietal is a distinctive skeletal element which has served as a basis for establishing the genus Albionbatrachus. Because little was known about developmental and individual variation of the frontoparietal, and its usefulness in delimiting genera and species has sometimes been doubted, we investigate its structure in Palaeobatrachus and Albionbatrachus by means of X-ray high resolution computer tomography (micro-CT). To infer the scope of variation present in the fossil specimens, we also examined developmental and interspecific variation in extant Xenopus. In adults of extinct taxa, the internal structure of the frontoparietal bone consists of a superficial and a basal layer of compact bone, with a middle layer of cancellous bone between them, much as in early amphibians. In Albionbatrachus, the layer of cancellous bone, consisting of small and large cavities, was connected with the dorsal, sculptured surface of the bone by a system of narrow canals; in Palaeobatrachus, the layer of cancellous bone and the canals connecting this layer with the dorsal surface of the frontoparietal were reduced. The situation in Palaeobatrachus robustus from the lower Miocene of France is intermediate-while external features support assignment to Palaeobatrachus, the inner structure is similar to that in Albionbatrachus. It may be hypothesized that sculptured frontoparietals with a well-developed layer of cancellous (i.e., vascularized) bone may indicate adaptation to a more terrestrial way of life, whereas a reduced cancellous layer might indicate a permanent water dweller.
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Anuros/anatomía & histología , Anuros/clasificación , Evolución Biológica , Hueso Frontal/anatomía & histología , Hueso Parietal/anatomía & histología , Animales , Femenino , Microtomografía por Rayos XRESUMEN
The evolutionary history of chameleons has been predominantly studied through phylogenetic approaches as the fossil register of chameleons is limited and fragmented. The poor state of preservation of these fossils has moreover led to the origin of numerous nomen dubia, and the identification of many chameleon fossils remains uncertain. We here examine chameleon fossil fragments from the Early Pliocene Varswater formation, exposed at the locality of Langebaanweg "E" Quarry along the southwestern coast of South Africa. Our aim was to explore whether these fossil fragments could be assigned to extant genera. To do so, we used geometric morphometric approaches based on microtomographic imaging of extant chameleons as well as the fossil fragments themselves. Our study suggests that the fossils from this deposit most likely represent at least two different forms that may belong to different genera. Most fragments are phenotypically dissimilar from the South African endemic genus Bradypodion and are more similar to other chameleon genera such as Trioceros or Kinyongia. However, close phenetic similarities between some of the fragments and the Seychelles endemic Archaius or the Madagascan genus Furcifer suggest that some of these fragments may not contain enough genus-specific information to allow correct identification. Other fragments such as the parietal fragments appear to contain more genus-specific information, however. Although our data suggest that the fossil diversity of chameleons in South Africa was potentially greater than it is today, this remains to be verified based on other and more complete fragments.
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Fósiles , Lagartos/anatomía & histología , Animales , Biodiversidad , Filogenia , Sudáfrica , Especificidad de la EspecieRESUMEN
The comparative vertebral morphology of different chamaeleonid genera has been generally neglected and some aspects such as the comparative anatomy of the neck region remain poorly known. The atlas and axis of all chamaeleonid genera (Brookesia, Rieppeleon, Archaius, Rhampholeon, Nadzikambia, Bradypodion, Chamaeleo, Calumma, Furcifer, Kinyongia, and Trioceros) are studied here. Considerable morphological differences are revealed. Additionally, some taxa exhibit sexual dimorphism in the atlas and axis. An extremely long, divided posterodorsal process is present in males of the Trioceros johnstoni + Trioceros jacksonii clade. The solid and well-developed morphology of the posterodorsal process in males of this taxon could reflect its competitive behavior-males fight with their horns and attempt to dislodge one another from branches during encounters. An additional area of insertion for the cervical musculature may indicate an incremental cervical musculature mass and cross sectional area that can add extra support and stability to the head and assist during combat involving lateral pushing. This character is not present in females. Heterochronic processes have played a role in the evolution of chamaeleonids, as evidenced in many characters of the atlas-axis complex. A new hypothesis of an anterior shifting of synapophyses of the axis is erected and a new derived anatomical structure of the parietal of Chamaeleo calyptratus is described (the processus parietalis inferior). The presence of the processus parietalis inferior is associated with the evolution of the dorsally elevated parietal crest.
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Vértebra Cervical Axis/anatomía & histología , Evolución Biológica , Atlas Cervical/anatomía & histología , Lagartos/anatomía & histología , Cráneo/anatomía & histología , Animales , Vértebra Cervical Axis/diagnóstico por imagen , Vértebra Cervical Axis/fisiología , Atlas Cervical/diagnóstico por imagen , Atlas Cervical/fisiología , Femenino , Imagenología Tridimensional , Lagartos/fisiología , Masculino , Factores Sexuales , Cráneo/diagnóstico por imagen , Cráneo/fisiología , Especificidad de la Especie , Sincrotrones , Microtomografía por Rayos XRESUMEN
Many scientists argue that our planet is undergoing a mass extinction event that is largely due to human influences. In this context, rediscoveries of species presumed to be extinct are encouraging and of great potential interest. During a 2003 expedition to New Caledonia, Bocourt's terrific skink, Phoboscincus bocourti, was unexpectedly rediscovered on a small islet by one of us. This skink species had been described from a single specimen collected around 1872 in New Caledonia. Since that time, however, no data on the species' biology, trophic interactions, or role in the ecosystem have been collected, making it difficult to follow the established conservation plan. In this study, we used a multidisciplinary approach involving natural history, anatomy, morphology, genetics, and stable isotopes to elucidate the ecology of Bocourt's terrific skink. Over the course of three different expeditions to the islet (total of 55 days across 2005 and 2012), we captured 4 individuals and observed another 4 individuals. The species' dentition and trophic ecology suggest that it is a top predator in its ecosystem and a major consumer of small terrestrial reptiles. Its high degree of genetic relatedness to another New Caledonian skink, which has a broad distribution, suggests that P. bocourti underwent genetic isolation at a geographical remote location, where dispersal or colonization was highly improbable. Moreover, the lack of genetic variation among the four individuals we captured may imply that a unique lineage, characterized by few inter-island exchanges, exists on the islet. Bocourt's terrific skink may be the largest terrestrial squamate predator alive in New Caledonia today. As a result, it is likely vulnerable to habitat modifications and especially the invasive rodents found on this islet. Further information is necessary to assess the conservation plans and practices in place as no concrete changes have been made since the species' rediscovery almost 10 years ago.
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Conservación de los Recursos Naturales , Lagartos , Animales , Dieta , Expediciones , Extinción Biológica , Cadena Alimentaria , Islas , Lagartos/genética , Microtomografía por Rayos XRESUMEN
Snakes are a very speciose group of squamates that adapted to various habitats and ecological niches. Their ecological diversity is of particular interest and functional demands associated with their various styles of locomotion are expected to result in anatomical specializations. In order to explore the potential adaptation of snakes to their environment we here analyze variation in vertebral structure at the microanatomical level in species with different locomotor adaptations. Vertebrae, being a major element of the snake body, are expected to display adaptations to the physical constraints associated with the different locomotor modes and environments. Our results revealed a rather homogenous vertebral microanatomy in contrast to what has been observed for other squamates and amniotes more generally. We here suggest that the near-absence of microanatomical specializations in snake vertebrae might be correlated to their rather homogeneous overall morphology and reduced range of morphological diversity, as compared to lizards. Thus, snakes appear to retain a generalist inner morphology that allows them to move efficiently in different environments. Only a few ecologically highly specialized taxa appear to display some microanatomical specializations that remain to be studied in greater detail.
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Ecosistema , Serpientes/anatomía & histología , Columna Vertebral/anatomía & histología , Animales , Serpientes/clasificación , Especificidad de la EspecieRESUMEN
What originally appeared to be only an external cast of an anuran 'mummy' from the Quercy Phosphorites (southwestern France) was described as Rana plicata during the 19th century. Its geographical provenance is only vaguely known; therefore its precise age within the Paleogene was uncertain. The taxon was erected on the basis of the external morphology of the specimen, which includes few diagnostic characters. As a further complication, the name Rana plicata was recently shown to be unavailable at the time of the description, and the name Rana cadurcorum was proposed as a replacement. In order to see whether internal features were fossilized, the fossil was CT scanned. This showed that a large part of the skeleton is preserved. Unexpectedly, the scans revealed that the skull of the mummy is almost identical to that of Thaumastosaurus gezei, another anuran from the late middle or late Eocene of the Quercy Phosphorites. The few observed differences are attributable to intraspecific and ontogenetic variation, and R. cadurcorum is a junior subjective synonym of T. gezei. The mummy is therefore probably from the same time interval as T. gezei. The latter was previously known only by its skull, but the mummy provides important information on the postcranial skeleton. Earlier assessments, based only on the skull, placed Thaumastosaurus close to South American hyloid anurans, but a new phylogenetic analysis including postcranial characters reveals ranoid affinities. This study exemplifies the usefulness of modern imaging technologies that allow non-destructive study of previously inaccessible internal anatomical features.
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Anuros/anatomía & histología , Fósiles , Microtomografía por Rayos X , Animales , Anuros/clasificación , Huesos/anatomía & histología , Huesos/diagnóstico por imagen , Paleontología , Filogenia , Cráneo/anatomía & histología , Factores de TiempoRESUMEN
Acoustic communication is widespread in animals. According to the sensory drive hypothesis [Endler JA (1993) Philos Trans R Soc Lond B Biol Sci 340(1292):215-225], communication signals and perceptual systems have coevolved. A clear illustration of this is the evolution of the tetrapod middle ear, adapted to life on land. Here we report the discovery of a bone conduction-mediated stimulation of the ear by wave propagation in Sechellophryne gardineri, one of the world's smallest terrestrial tetrapods, which lacks a middle ear yet produces acoustic signals. Based on X-ray synchrotron holotomography, we measured the biomechanical properties of the otic tissues and modeled the acoustic propagation. Our models show how bone conduction enhanced by the resonating role of the mouth allows these seemingly deaf frogs to communicate effectively without a middle ear.
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Anuros/fisiología , Conducción Ósea/fisiología , Oído Interno/anatomía & histología , Audición/fisiología , Modelos Biológicos , Animales , Fenómenos Biomecánicos , Boca/anatomía & histología , Boca/fisiología , Sincrotrones , Vocalización Animal/fisiologíaRESUMEN
The occlusal morphology of the teeth is mostly determined by the enamel-dentine junction morphology; the enamel-dentine junction plays the role of a primer and conditions the formation of the occlusal enamel reliefs. However, the accretion of the enamel cap yields thickness variations that alter the morphology and the topography of the enamel-dentine junction (i.e., the differential deposition of enamel by the ameloblasts create an external surface that does not necessarily perfectly parallel the enamel-dentine junction). This self-reliant influence of the enamel on tooth morphology is poorly understood and still under-investigated. Studies considering the relationship between enamel and dentine morphologies are rare, and none of them tackled this relationship in a quantitative way. Major limitations arose from: (1) the difficulties to characterize the tooth morphology in its comprehensive tridimensional aspect and (2) practical issues in relating enamel and enamel-dentine junction quantitative traits. We present new aspects of form representation based exclusively on 3D analytical tools and procedures. Our method is applied to a set of 21 unworn upper second molars belonging to eight extant anthropoid genera. Using geometrical analysis of polygonal meshes representatives of the tooth form, we propose a 3D dataset that constitutes a detailed characterization of the enamel and of the enamel-dentine junction morphologies. Also, for the first time, to our knowledge, we intend to establish a quantitative method for comparing enamel and enamel-dentine junction surfaces descriptors (elevation, inclination, orientation, etc.). New indices that allow characterizing the occlusal morphology are proposed and discussed. In this note, we present technical aspects of our method with the example of anthropoid molars. First results show notable individual variations and taxonomic heterogeneities for the selected topographic parameters and for the pattern and strength of association between enamel-dentine junction and enamel, the enamel cap altering in different ways the "transcription" of the enamel-dentine junction morphology.
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Imagenología Tridimensional , Diente/diagnóstico por imagen , Animales , Primates , RadiografíaRESUMEN
Amphibian declines are seen as an indicator of the onset of a sixth mass extinction of life on earth. Because of a combination of factors such as habitat destruction, emerging pathogens and pollutants, over 156 amphibian species have not been seen for several decades, and 34 of these were listed as extinct by 2004. Here we report the rediscovery of the Hula painted frog, the first amphibian to have been declared extinct. We provide evidence that not only has this species survived undetected in its type locality for almost 60 years but also that it is a surviving member of an otherwise extinct genus of alytid frogs, Latonia, known only as fossils from Oligocene to Pleistocene in Europe. The survival of this living fossil is a striking example of resilience to severe habitat degradation during the past century by an amphibian.
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Anuros/anatomía & histología , Fósiles , Animales , Huesos/anatomía & histología , Europa (Continente) , Femenino , Israel , Funciones de Verosimilitud , Masculino , Datos de Secuencia Molecular , FilogeniaRESUMEN
Chameleons are highly specialized and mostly arboreal lizards characterized by a suite of derived characters. The grasping feet and tail are thought to be related to the arboreal lifestyle of chameleons, yet specializations for grasping are thought to exhibit a trade-off with running ability. Indeed, previous studies have demonstrated a trade-off between running and clinging performance, with faster species being poorer clingers. Here we investigate the presence of trade-offs by measuring running and grasping performance in four species of chameleon belonging to two different clades (Chamaeleo and Bradypodion). Within each clade we selected a largely terrestrial species and a more arboreal species to test whether morphology and performance are related to habitat use. Our results show that habitat drives the evolution of morphology and performance but that some of these effects are specific to each clade. Terrestrial species in both clades show poorer grasping performance than more arboreal species and have smaller hands. Moreover, hand size best predicts gripping performance, suggesting that habitat use drives the evolution of hand morphology through its effects on performance. Arboreal species also had longer tails and better tail gripping performance. No differences in sprint speed were observed between the two Chamaeleo species. Within Bradypodion, differences in sprint speed were significant after correcting for body size, yet the arboreal species were both better sprinters and had greater clinging strength. These results suggest that previously documented trade-offs may have been caused by differences between clades (i.e. a phylogenetic effect) rather than by design conflicts between running and gripping per se.