Taxonomic Interference Associated with Phonemic Paraphasias in Agrammatic Primary Progressive Aphasia.

Phonemic paraphasias are thought to reflect phonological (post-semantic) deficits in language production. Here we present evidence that phonemic paraphasias in non-semantic primary progressive aphasia (PPA) may be associated with taxonomic interference. Agrammatic and logopenic PPA patients and control participants performed a word-to-picture visual search task where they matched a stimulus noun to 1 of 16 object pictures as their eye movements were recorded. Participants were subsequently asked to name the same items. We measured taxonomic interference (ratio of time spent viewing related vs. unrelated foils) during the search task for each item. Target items that elicited a phonemic paraphasia during object naming elicited increased taxonomic interference during the search task in agrammatic but not logopenic PPA patients. These results could reflect either very subtle sub-clinical semantic distortions of word representations or partial degradation of specific phonological word forms in agrammatic PPA during both word-to-picture matching (input stage) and picture naming (output stage). The mechanism for phonemic paraphasias in logopenic patients seems to be different and to be operative at the pre-articulatory stage of phonological retrieval. Glucose metabolic imaging suggests that degeneration in the left posterior frontal lobe and left temporo-parietal junction, respectively, might underlie these different patterns of phonemic paraphasia.

Variability in blood oxygen level dependent (BOLD) signal in patients with stroke-induced and primary progressive aphasia.

Although fMRI is increasingly used to assess language-related brain activation in patients with aphasia, few studies have examined the hemodynamic response function (HRF) in perilesional, and contralesional areas of the brain. In addition, the relationship between HRF abnormalities and other variables such as lesion size and severity of aphasia has not been explored. The objective of this study was to investigate changes in HRF signal during language-related neural activation in patients with stroke-induced aphasia (SA). We also examined the status of the HRF in patients with aphasia due to nonvascular etiology, namely, primary progressive aphasia (PPA). Five right handed SA patients, three PPA patients, and five healthy individuals participated in the study. Structural damage was quantified with T1-weighted MR images. Functional MR imaging was performed with long trial event-related design and an overt naming task to measure BOLD signal time to peak (TTP) and percent signal change (ΔS). In SA patients, the average HRF TTP was significantly delayed in the left hemisphere regions involved in naming compared to healthy participants and PPA patients. However, ΔS was not different in SA patients compared to the other two groups. Delay in HRF TTP in the left hemisphere naming network of SA patients was correlated with lesion size and showed a negative correlation with global language function. There were no significant differences in the HRF TTP and ΔS in the right hemisphere homologues of the naming network or in the left and the right occipital control regions across the three groups. In PPA patients, HRF had a normal pattern. Our results indicate that abnormal task-related HRF is primarily found in the left hemisphere language network of SA patients and raise the possibility that abnormal physiology superimposed on structural damage may contribute to the clinical deficit. Follow-up investigations in a larger sample of age-matched healthy individuals, SA, and PPA patients will be needed to further confirm and extend our findings.

Kinetics of styrene biodegradation in synthetic wastewaters using an industrial activated sludge.

Kinetics of styrene biodegradation in synthetic wastewaters, containing either styrene or styrene together with ethanol, by an industrial activated sludge obtained from the wastewater treatment unit of a petrochemical complex was studied. The kinetic data could be fitted using the Haldane kinetic model. This model was previously used to predict kinetic data for biodegradation of styrene by pure or mixed microbial cultures isolated from biofilters, but the values of the model parameters reported in these studies was substantially different from that obtained for the industrial activated sludge. The presence of ethanol did not affect the kinetics of styrene biodegradation by the industrial activated sludge; however, it increased the rates of styrene biodegradation due to the resulting higher microbial growth rates. Styrene concentration was found to affect the specific growth rate in a manner similar to its effect on the styrene degradation rate. No lag phase was observed in styrene biodegradation by industrial activated sludge for styrene concentrations up to 100mg/L. Lag phase was observed for municipal activated sludge even at 50mg/L styrene concentration but the rate of styrene biodegradation after the lag phase was similar to that achieved by the industrial activated sludge.

Long-term operation of submerged membrane bioreactor (MBR) for the treatment of synthetic wastewater containing styrene as volatile organic compound (VOC): Effect of hydraulic retention time (HRT).

In this study, the membrane bioreactor (MBR) was utilized to remove styrene from a synthetic wastewater having a chemical oxygen demand (COD) and styrene concentration of 1500 mg/L and 50 mg/L, respectively. At two hydraulic retention times (HRTs) of 24 h and 18 h, the MBR was operated for a period in excess of 100 days. The HRT effects were studied and it was found out that the removal efficiency of COD and styrene for both HRTs was consistently higher than 99%. Unlike conventional activated sludge processes (CASPs), no styrene was detected in the exhaust air, which meant that biodegradation was the major styrene removal mechanism at both HRTs. The transmembrane pressure (TMP) profile during the operation of the MBR showed a fairly low and constant TMP up to day 70, after which, the TMP showed a dramatic rise, as a result of the occurrence of severe membrane fouling. It was thought that an increase in styrene loading rate, when HRT was reduced to 18 h, resulted in the release of extracellular polymeric substance (EPS) from the bacterial cells, which in turn was responsible for the rise in soluble microbial product (SMP) and sludge deflocculation. The severe fouling observed during operation of MBR at HRT of 18 h was attributed to the rise in SMP concentrations and decrease in mean floc size and increase in the proportion of small particles in the activated sludge.

Hemodynamic response function in patients with stroke-induced aphasia: implications for fMRI data analysis.

Functional MRI is based on changes in cerebral microvasculature triggered by increased neuronal oxidative metabolism. This change in blood flow follows a pattern known as the hemodynamic response function (HRF), which typically peaks 4-6 s following stimulus delivery. However, in the presence of cerebrovascular disease the HRF may not follow this normal pattern, due to either the temporal signal to noise (tSNR) ratio or delays in the HRF, which may result in misinterpretation or underestimation of fMRI signal. The present study examined the HRF and SNR in five individuals with aphasia resulting from stroke and four unimpaired participants using a lexical decision task and a long trial event-related design. T1-weighted images were acquired using an MP-RAGE sequence and BOLD T2*-weighted images were acquired using Echo Planar Imaging to measure time to peak (TTP) in the HRF. Data were analyzed using Brain Voyager in four anatomic regions known to be involved in language processing: Broca's area and the posterior perisylvian network (PPN) (including Wernicke's area, the angular and supramarginal gyri) and right hemisphere homologues of these regions. The occipital area also was examined as a control region. Analyses showed that the TTP in three out of five patients in the left perisylvian area was increased significantly as compared to normal individuals and the left primary visual cortex in the same patients. In two other patients no significant delays were detected. We also found that the SNR for BOLD signal detection may by insufficient in damaged areas. These findings indicate that obtaining physiologic (TTP) and quality assurance (tSNR) information is essential for studying activation patterns in brain-damaged patients in order to avoid errors in interpretation of the data. An example of one such misinterpretation and the need for alternative data analysis strategies is discussed.

Application of the central composite design and response surface methodology to the advanced treatment of olive oil processing wastewater using Fenton's peroxidation.

The central composite design (CCD) technique was used to study the effect of the Fenton's peroxidation on the removal of organic pollutants from olive oil mill wastewater (OMW). The ratio of hydrogen peroxide-to-Fe(II) (x1) was between 1.67 and 8.33. Fe(II) concentration was constant at 0.03 M while the H2O2 concentration was set at three levels: 0.05, 0.15 and 0.25 M. Based on the molarity ratio, the selected ratio were in the low range of Fe(II)-to-H2O2 ratio (<<1). While based on the wt/wt ratio, the tested Fe(II)-to-H2O2 ratios were in the range of < or =1:5. pH (x2) was between 3 and 5. The concentration of OMW (x3) was varied between 40 and 100%. The influence of these three independent variables on the four dependent variables, i.e. COD, total phenolics (TP), color and aromaticity removal was evaluated using a second-order polynomial multiple regression model. Analysis of variance (ANOVA) showed a high coefficient of determination (R2) value of 0.902-0.998, thus ensuring a satisfactory adjustment of the second-order regression model with the experimental data. H2O2-to-Fe(II) ratio had significant effect on all the four dependent variables. The positive sign for the regression coefficient of this regressor variable indicated that the level of the pollutant removal increased with the increased levels of factor x1 from 1.67 to 8.33 and this effect was the most pronounced for TP removal. pH had also significant effect on the pollutant removal and the effect was the most noticeable for TP reduction. The negative coefficient of this variable (pH) indicated that level of the pollutant removal decreased as the pH increased from 3 to 5. The negative coefficient of the interaction between variable x1 and x2 indicated that a simultaneous increase in H2O2-to-Fe(II) ratio with decrease in the pH of the reaction led to an increase in the COD, TP and color removal. Quadratic models were predicted for the response variable, i.e. pollutant removal, and the maximum model-predicted removals were 56, 100, 33 and 32% for COD, TP, color and aromaticity, respectively. Optimum conditions for this wastewater treatment was obtained based on the performance of the Fenton's peroxidation in the experiment where the H2O2-to-Fe(II) ratio was at its high level (8.33) and the pH and OMW concentration were 4 and 70%, respectively.