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Biol Reprod ; 66(3): 580-8, 2002 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-11870061

RESUMEN

We used okadaic acid (OA), a potent inhibitor of protein phosphatases 1 and 2A, to study the regulatory effects of protein phosphatases on mitogen-activated protein (MAP) kinase phosphorylation, morphological changes in the nucleus, and microtubule assembly during pig oocyte maturation and fertilization in vitro. When germinal vesicle (GV) stage oocytes were exposed to OA, MAP kinase phosphorylation was greatly accelerated, being fully activated at 10 min. However, MAP kinase was dephosphorylated by long-term (>20 h) exposure to OA. Correspondingly, premature chromosome condensation and GV breakdown were accelerated, whereas meiotic spindle assembly and meiotic progression beyond metaphase I stage were inhibited. OA also quickly reversed the inhibitory effects of butyrolactone I, a specific inhibitor of maturation-promoting factor (MPF), on MAP kinase phosphorylation and meiosis resumption. Treatment of metaphase II oocytes triggered metaphase II spindle elongation and disassembly as well as chromosome alignment disruption. OA treatment of fertilized eggs resulted in prompt phosphorylation of MAP kinase, disassembly of microtubules around the pronuclear area, chromatin condensation, and pronuclear membrane breakdown, but inhibited further cleavage. Our results suggest that inhibition of protein phosphatases promptly phosphorylates MAP kinase, induces premature chromosome condensation and meiosis resumption as well as pronucleus breakdown, but inhibits spindle organization and suppresses microtubule assembly by sperm centrosomes in pig oocytes and fertilized eggs.


Asunto(s)
4-Butirolactona/análogos & derivados , Ciclo Celular , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Oocitos/metabolismo , Oocitos/ultraestructura , Fosfoproteínas Fosfatasas/metabolismo , Porcinos , 4-Butirolactona/farmacología , Animales , Cromatina/efectos de los fármacos , Cromatina/ultraestructura , Fase de Segmentación del Huevo/efectos de los fármacos , Activación Enzimática/efectos de los fármacos , Inhibidores Enzimáticos/farmacología , Femenino , Fertilización In Vitro/veterinaria , Factor Promotor de Maduración/antagonistas & inhibidores , Meiosis , Metafase , Microtúbulos/efectos de los fármacos , Microtúbulos/ultraestructura , Membrana Nuclear/efectos de los fármacos , Membrana Nuclear/ultraestructura , Ácido Ocadaico/farmacología , Fosfoproteínas Fosfatasas/antagonistas & inhibidores , Fosforilación , Huso Acromático/efectos de los fármacos
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