RESUMEN
BACKGROUND: The functional activity of trimeric guanine-nucleotide-binding proteins (G-proteins) represents an essential step in linking and regulation of the opioid receptor (mu-, delta- and kappa-OR)-initiated signaling pathways. Theoretical basis and/or molecular mechanism(s) of opioid tolerance and addiction proceeding in the central nervous system were not studied in the forebrain cortex of mammals with respect to quantitative analysis of opioid-stimulated trimeric G-protein activity. MATERIAL/METHODS: G-protein activity was measured in PercollR-purified plasma membranes (PM) isolated from the frontal brain cortex of control and morphine-treated rats by both high-affinity [32P]GTPase and [35S]GTPgammaS binding assays. Exposition to morphine was performed by intra-muscular application of this drug. Control animals were injected with sterile PBS. RESULTS: Both mu-OR (DAMGO)- and delta-OR (DADLE)-responses were clearly desensitized in PM isolated from morphine-treated rats; kappa-OR (U-69593)- and baclofen (GABAB-R)-stimulated [35S]GTPgammaS binding was unchanged, indicating the specificity of the morphine effect. Under such conditions, the amount of G-protein alpha subunits was unchanged. The order of efficacy DADLE>DAMGO>U-69593 was the same in control and morphine-treated PM. Behavioral tests indicated that morphine-treated animals were fully drug-dependent and developed tolerance to subsequent drug addition. CONCLUSIONS: Prolonged exposure of rats to high doses of morphine results in decrease of the over-all output of OR-stimulated G-protein activity in the forebrain cortex but does not decrease the amount of these regulatory proteins. These data support the view that the mechanism of the long-term adaptation to high doses of morphine is primarily based on desensitization of OR-response preferentially oriented to mu-OR and delta-OR.
Asunto(s)
Adaptación Fisiológica/efectos de los fármacos , Corteza Cerebral/metabolismo , Subunidades alfa de la Proteína de Unión al GTP/metabolismo , Morfina/administración & dosificación , Morfina/farmacología , Receptores Opioides delta/metabolismo , Receptores Opioides mu/metabolismo , Animales , Baclofeno/farmacología , Conducta Animal/efectos de los fármacos , Membrana Celular/efectos de los fármacos , Membrana Celular/enzimología , Corteza Cerebral/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Tolerancia a Medicamentos , Encefalina Ala(2)-MeFe(4)-Gli(5)/farmacología , Guanosina 5'-O-(3-Tiotrifosfato)/metabolismo , Guanosina Difosfato/metabolismo , Masculino , Ratas , Ratas Wistar , Receptores Opioides delta/agonistas , Receptores Opioides mu/agonistas , Síndrome de Abstinencia a Sustancias/fisiopatología , Factores de TiempoRESUMEN
Regulator of G protein signaling (RGS) proteins function as GTPase-activating proteins (GAPs) for the alpha-subunit of heterotrimeric G proteins. Several RGS proteins have been found to interact with 14-3-3 proteins. The 14-3-3 protein binding inhibits the GAP function of RGS proteins presumably by blocking their interaction with G(alpha) subunit. Since RGS proteins interact with G(alpha) subunits through their RGS domains, it is reasonable to assume that the 14-3-3 protein can either sterically occlude the G(alpha) interaction surface of RGS domain and/or change its structure. In this work, we investigated whether the 14-3-3 protein binding affects the structure of RGS3 using the time-resolved tryptophan fluorescence spectroscopy. Two single-tryptophan mutants of RGS3 were used to study conformational changes of RGS3 molecule. Our measurements revealed that the 14-3-3 protein binding induces structural changes in both the N-terminal part and the C-terminal RGS domain of phosphorylated RGS3 molecule. Experiments with the isolated RGS domain of RGS3 suggest that this domain alone can, to some extent, interact with the 14-3-3 protein in a phosphorylation-independent manner. In addition, a crystal structure of the RGS domain of RGS3 was solved at 2.3A resolution. The data obtained from the resolution of the structure of the RGS domain suggest that the 14-3-3 protein-induced conformational change affects the region within the G(alpha)-interacting portion of the RGS domain. This can explain the inhibitory effect of the 14-3-3 protein on GAP activity of RGS3.
Asunto(s)
Proteínas 14-3-3/química , Proteínas 14-3-3/metabolismo , Proteínas de Unión al GTP/química , Proteínas de Unión al GTP/metabolismo , Proteínas Activadoras de GTPasa/química , Proteínas Activadoras de GTPasa/metabolismo , Secuencia de Aminoácidos , Sustitución de Aminoácidos , Sitios de Unión , Cristalografía por Rayos X , Transferencia Resonante de Energía de Fluorescencia , Proteínas de Unión al GTP/genética , Proteínas Activadoras de GTPasa/genética , Humanos , Técnicas In Vitro , Modelos Moleculares , Complejos Multiproteicos , Mutagénesis Sitio-Dirigida , Fosforilación , Dominios y Motivos de Interacción de Proteínas , Estabilidad Proteica , Estructura Terciaria de Proteína , Subunidades de Proteína , Proteínas RGS , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Espectrometría de Fluorescencia , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Triptófano/químicaRESUMEN
This study aimed to determine whether sustained stimulation with thyrotropin-releasing hormone (TRH), a peptide with important physiological functions, can possibly affect expression of plasma membrane proteins in HEK293 cells expressing high levels of TRH receptor and G(11)alpha protein. Our previous experiments using silver-stained two-dimensional polyacrylamide gel electrophoretograms did not reveal any significant changes in an overall composition of membrane microdomain proteins after long-term treatment with TRH of these cells (Matousek et al. 2005 Cell Biochem Biophys 42: 21-40). Here we used a purified plasma membrane fraction prepared by Percoll gradient centrifugation and proteins resolved by 2D electrophoresis were stained with SYPRO Ruby gel stain. The high enrichment in plasma membrane proteins of this preparation was confirmed by a multifold increase in the number of TRH receptors and agonist stimulated G-protein activity, compared to postnuclear supernatant. By a combination of these approaches we were able to determine a number of clearly discernible protein changes in the plasma membrane-enriched fraction isolated from cells treated with TRH (1 x 10(-5) M, 16 h): 4 proteins disappeared, the level of 18 proteins decreased and the level of 39 proteins increased. Our concomitant immunochemical determinations also indicated a clear down-regulation of G(q/11)alpha proteins in preparations from hormone-treated cells. In parallel, we observed decrease in caspase 3 and alterations in some other apoptotic marker proteins, which were in line with the presumed antiapoptotic effect of TRH.
Asunto(s)
Expresión Génica/fisiología , Proteínas de la Membrana/metabolismo , Proteínas Nucleares/metabolismo , Proteínas Serina-Treonina Quinasas/metabolismo , Receptores de Hormona Liberadora de Tirotropina/metabolismo , Hormona Liberadora de Tirotropina/metabolismo , Apoptosis/fisiología , Western Blotting , Línea Celular , Electroforesis en Gel Bidimensional , Expresión Génica/efectos de los fármacos , Humanos , Proteínas de la Membrana/efectos de los fármacos , Hormona Liberadora de Tirotropina/farmacologíaRESUMEN
BACKGROUND: Heterotrimeric guanine nucleotide-binding proteins (G proteins) play an essential role in linking cell-surface receptors to effector proteins at the plasma membrane. The functional activities of G proteins in various plasma membrane compartments remain to be elucidated. MATERIAL/METHODS: Plasma membranes from rat cerebral cortex were isolated on Percoll and fractionated by sucrose-density gradient. Fractions were screened for plasma membrane markers and signaling molecules. G-protein activity was determined by agonist-stimulated gamma-32P-GTPase or 35S-GTPgammaS binding. The largest content of markers was found at the 35% to 40% (w/v) sucrose interface. This fraction was defined as the bulk of the plasma membrane. The low-density plasma membrane fraction was localized in 15% to 20% (w/v) sucrose. RESULTS: Both bulk and low-density plasma membrane fractions were characterized by high levels of nonspecific, low-affinity GTPase activity and basal, high-affinity GTPase activity. Baclofen-stimulated GTPase activity was twice as high in the bulk fraction as in the low-density fraction. The effect of other G protein-coupled receptor agonists was not significant. 35S-GTPgammaS saturation-binding experiments measured with increasing concentrations of GDP revealed high-affinity sites that were clearly distinguishable from basal binding and responded to agonists in the following order of efficacy: baclofen >(DADLE) >(DAMGO) >U-69593. CONCLUSIONS: The method presented here describes a straightforward method for the isolation of clearly defined plasma membrane preparations from rat brain cortex. Quantitative assessment of G-protein activity, particularly the high basal activity, differs from that reported in membrane fractions from HEK 293 cells.
Asunto(s)
Corteza Cerebral/metabolismo , Proteínas de Unión al GTP/metabolismo , Adenilil Ciclasas/metabolismo , Animales , Baclofeno/farmacología , Western Blotting , Línea Celular , Membrana Celular/metabolismo , Corteza Cerebral/enzimología , Electroforesis en Gel de Poliacrilamida , Activación Enzimática , GTP Fosfohidrolasas/metabolismo , Proteínas de Unión al GTP/agonistas , Guanosina 5'-O-(3-Tiotrifosfato)/metabolismo , Humanos , Masculino , Ratas , Ratas WistarRESUMEN
We monitored the radioligand-binding characteristics of thyrotropin-releasing hormone (TRH) receptors, functional activity of G(q/11)alpha proteins, and functional status of the whole signaling cascade in HEK293 expressing high levels of TRH receptors and G(11)alpha. Our analyses indicated that disruption of plasma membrane microdomains by cholesterol depletion did not markedly influence the binding parameters of TRH receptors, but it altered efficacy of signal transduction. The functional coupling between TRH receptor and G(q/11)alpha was assessed by agonist-stimulated [(35)S]GTPgammaS binding, and results of these measurements pointed out to significantly lower potency of TRH to mediate G protein activation in the plasma membrane fraction isolated from cholesterol-depleted cells; there was a shift in sensitivity by one order of magnitude to the higher concentrations. A markedly lower sensitivity to stimulation with TRH was also observed in our experiments dealing with determination of hormone-induced Ca(2+) response. These data suggest that the intact structure of plasma membranes is an important optimum signal transduction initiated by TRH receptors and mediated by G(q/11)alpha proteins.
Asunto(s)
Membrana Celular/metabolismo , Colesterol/fisiología , Subunidades alfa de la Proteína de Unión al GTP Gq-G11/metabolismo , Microdominios de Membrana/metabolismo , Receptores de Hormona Liberadora de Tirotropina/metabolismo , Transducción de Señal , Hormona Liberadora de Tirotropina/metabolismo , Animales , Calcio/metabolismo , Línea Celular , Membrana Celular/ultraestructura , Guanosina 5'-O-(3-Tiotrifosfato)/metabolismo , Humanos , LigandosRESUMEN
Adenylyl cyclase (AC) in brain cortex from young (12-day-old) rats exhibits markedly higher activity than in adult (90-day-old) animals. In order to find some possibly different regulatory features of AC in these two age groups, here we modulated AC activity by dithiothreitol (DTT), Fe(2+), ascorbic acid and suramin. We did not detect any substantial difference between the effects of all these tested agents on AC activity in cerebrocortical membranes from young and adult rats, and the enzyme activity was always about two-fold higher in the former preparations. Nevertheless, several interesting findings have come out of these investigations. Whereas forskolin- and Mn(2+)-stimulated AC activity was significantly enhanced by the addition of DTT, increased concentrations of Fe(2+) ions or ascorbic acid substantially suppressed the enzyme activity. Lipid peroxidation induced by suitable combinations of DTT/Fe(2+) or by ascorbic acid did not influence AC activity. We have also observed that PKC- or protein tyrosine kinase-mediated phosphorylation apparently does not play any significant role in different activity of AC determined in cerebrocortical preparations from young and adult rats. Our experiments analysing the presumed modulatory role of suramin revealed that this pharmacologically important drug may act as a direct inhibitor of AC. The enzyme activity was diminished to the same extent by suramin in membranes from both tested age groups. Our present data show that AC is regulated similarly in brain cortex from both young and adult rats, but its overall activity is much lower in adulthood.
Asunto(s)
Inhibidores de Adenilato Ciclasa , Adenilil Ciclasas/metabolismo , Envejecimiento , Encéfalo/enzimología , Corteza Cerebral/enzimología , Suramina/química , Adenilil Ciclasas/química , Compuestos de Aluminio/farmacología , Animales , Ácido Ascórbico/química , Ácido Ascórbico/farmacología , Encéfalo/metabolismo , Membrana Celular/metabolismo , Corteza Cerebral/metabolismo , Colforsina/farmacología , Ditiotreitol/farmacología , Relación Dosis-Respuesta a Droga , Fluoruros/farmacología , Guanosina 5'-O-(3-Tiotrifosfato)/metabolismo , Hierro/química , Peroxidación de Lípido , Masculino , Manganeso/farmacología , Fosforilación , Proteína Quinasa C/metabolismo , Proteínas Tirosina Quinasas/metabolismo , Ratas , Ratas Wistar , Suramina/metabolismo , Suramina/farmacología , Factores de TiempoRESUMEN
The number and affinity of GABA(B) receptors (assayed by the specific antagonist [(3)H]CGP54626A) was unchanged when compared in carefully washed cerebrocortical membranes from young (12-day-old) and adult (90-day-old) rats. In contrast, high-affinity GTPase activity, both basal and baclofen-stimulated was significantly higher (by 45% and 56%, respectively) in adult than in young rats. Similar results were obtained by concomitant determination of agonist (baclofen)-stimulated GTP gamma S binding. Under standard conditions, baclofen-stimulated GTPase activity was further considerably enhanced by exogenously added regulator of G protein function, RGS1, but not by RGS16. RGS16 was able to affect agonist-stimulated GTPase activity only in the presence of markedly increase substrate (GTP) concentrations. RGS1 alone slightly increased GTPase activity in adult rats, but neither RGS1 nor RGS16 influenced GTPase activity in membrane preparations isolated from young animals. These findings indicate increasing functional activity of trimeric G protein(s) involved in GABAergic transmission in the developing rat brain cortex and suggest a high potential of RGS1 in regulation of high-affinity GTPase activity.
Asunto(s)
Baclofeno/farmacología , Corteza Cerebral/efectos de los fármacos , Proteínas de Unión al GTP/fisiología , Envejecimiento/fisiología , Análisis de Varianza , Animales , Animales Recién Nacidos , Corteza Cerebral/crecimiento & desarrollo , Relación Dosis-Respuesta a Droga , Interacciones Farmacológicas , Agonistas del GABA/farmacología , GTP Fosfohidrolasas/metabolismo , Guanosina 5'-O-(3-Tiotrifosfato)/farmacología , Masculino , Compuestos Organofosforados/farmacocinética , Unión Proteica/efectos de los fármacos , Unión Proteica/fisiología , Proteínas/farmacología , Proteínas RGS/farmacología , Ensayo de Unión Radioligante/métodos , Ratas , Tritio/farmacocinéticaRESUMEN
Low-density membrane fragments (domains) were separated from the bulk of plasma membranes of human embryonic kidney (HEK)293 cells expressing a delta-opioid (DOP) receptor-Gi1alpha fusion protein by drastic homogenization and flotation on equilibrium sucrose density gradients. The functional activity of trimeric G proteins and capacity of the DOP receptor to stimulate both the fusion protein-linked Gi1alpha and endogenous pertussis-toxin sensitive G proteins was measured as d-Ala2, d-Leu5-enkephalin stimulated high-affinity GTPase or guanosine-5'-[gamma-35S]triphosphate ([35S]GTPgammaS) binding. The maximum d-Ala2-d-Leu5 enkephalin (DADLE)-stimulated GTPase was two times higher in low-density membrane fragments than in bulk of plasma membranes; 58 and 27 pmol/mg/min, respectively. The same difference was obtained for [35S]GTPgammaS binding. Contrarily, the low-density domains contained no more than half the DOP receptor binding sites (Bmax = 6.6 pmol/mg versus 13.6 pmol/mg). Thus, when corrected for expression levels of the receptor, low-density domains exhibited four times higher agonist-stimulated GTPase and [35S]GTPgammaS binding than the bulk plasma membranes. The regulator of G protein signaling RGS1, enhanced further the G protein functional activity but did not remove the difference between domain-bound and plasma membrane pools of G protein. The potency of the agonist in functional studies and the affinity of specific [3H]DADLE binding to the receptor were, however, the same in both types of membranes - EC50 = 4.5 +/- 0.1 x 10(-8) and 3.2 +/- 1.4 x 10(-8) m for GTPase; Kd = 1.2 +/- 0.1 and 1.3 +/- 0.1 nm for [3H]DADLE radioligand binding assay. Similar results were obtained when sodium bicarbonate was used for alkaline isolation of membrane domains. By contrast, detergent-insensitive membrane domains isolated following treatment of cells with Triton X100 exhibited no DADLE-stimulated GTPase or GTPgammaS binding. Functional coupling between the DOP receptor and cognate G proteins was also blocked by high-energy ultrasound and repeated freezing-thawing. Our data indicate, for the first time, that membrane domains isolated using 'detergent-free' procedures exhibit higher efficiency of coupling between a G protein-coupled receptor and its corresponding G protein(s) than bulk plasma membranes. Detergent-extraction diminishes these interactions, even when the receptor and G proteins are physically tethered together.
Asunto(s)
Membrana Celular/química , Proteínas de Unión al GTP Heterotriméricas/química , Receptores Opioides delta/química , Línea Celular , Centrifugación por Gradiente de Densidad , Detergentes/química , Detergentes/farmacología , Relación Dosis-Respuesta a Droga , Leucina Encefalina-2-Alanina/farmacología , Activación Enzimática/efectos de los fármacos , Activación Enzimática/fisiología , Guanosina 5'-O-(3-Tiotrifosfato)/farmacocinética , Humanos , Riñón/química , Riñón/citología , Unión Proteica/efectos de los fármacos , Unión Proteica/fisiología , Receptores Opioides delta/agonistas , Transducción de Señal/efectos de los fármacos , Transducción de Señal/fisiología , Solubilidad/efectos de los fármacos , Sonicación , Fracciones Subcelulares/químicaRESUMEN
In vitro experiments suggest that stimulation of lipolysis by catecholamines in adipocytes depends on the energy status of these cells. We tested whether mitochondrial uncoupling proteins (UCPs) that control the efficiency of ATP production could affect lipolysis and noradrenaline signalling in white fat in vivo. The lipolytic effect of noradrenaline was lowered by ectopic UCP1 in white adipocytes of aP2-Ucp1 transgenic mice, overexpressing the UCP1 gene from the aP2 gene promoter, reflecting the magnitude of UCP1 expression, the impaired stimulation of cAMP levels by noradrenaline and the reduction of the ATP/ADP ratio in different fat depots. Thus only subcutaneous but not epididymal fat was affected. UCP1 also down-regulated the expression of hormone-sensitive lipase and lowered its activity, and altered the expression of trimeric G-proteins in adipocytes. The adipose tissue content of the stimulatory G-protein alpha subunit was increased while that of the inhibitory G-protein alpha subunits decreased in response to UCP1 expression. Our results support the idea that the energy status of cells, and the ATP/ADP ratio in particular, modulates the lipolytic effects of noradrenaline in adipose tissue in vivo. They also demonstrate changes at the G-protein level that tend to overcome the reduction of lipolysis when ATP level in adipocytes is low. Therefore, respiratory uncoupling may exert a broad effect on hormonal signalling in adipocytes.
Asunto(s)
Tejido Adiposo/metabolismo , Proteínas Portadoras/fisiología , Proteínas de Unión al GTP/metabolismo , Lipólisis , Proteínas de la Membrana/fisiología , Norepinefrina/metabolismo , Adenosina Difosfato/metabolismo , Adenosina Trifosfato/metabolismo , Tejido Adiposo/enzimología , Animales , Secuencia de Bases , Proteínas Portadoras/genética , AMP Cíclico/metabolismo , Cartilla de ADN , Regulación Enzimológica de la Expresión Génica , Genotipo , Hepatocitos/enzimología , Canales Iónicos , Lipasa/genética , Masculino , Proteínas de la Membrana/genética , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Proteínas Mitocondriales , Proteína Desacopladora 1RESUMEN
Current strategies for cell-based screening generally focus on the development of highly specific assays, which require an understanding of the nature of the signaling molecules and cellular pathways involved. In contrast, changes in temperature of cells provides a measure of altered cellular metabolism that is not stimulus specific and hence could have widespread applications in cell-based screening of receptor agonists and antagonists, as well as in the assessment of toxicity of new lead compounds. Consequently, we have developed a micromachined nanocalorimetric biological sensor using a small number of isolated living cells integrated within a subnanoliter format, which is capable of detecting 13 nW of generated power from the cells, upon exposure to a chemical or pharmaceutical stimulus. The sensor comprises a 10-junction gold and nickel thermopile, integrated on a silicon chip which was back-etched to span a 800-nm-thick membrane of silicon nitride. The thin-film membrane, which supported the sensing junctions of the thermoelectric transducer, gave the system a temperature resolution of 0.125 mK, a low heat capacity of 1.2 nJ mK(-1), and a rapid (unfiltered) response time of 12 ms. The application of the system in ultra-low-volume cell-based assays could provide a rapid endogenous screen. It offers important additional advantages over existing methods in that it is generic in nature, it does not require the use of recombinant cell lines or of detailed assay development, and finally, it can enable the use of primary cell lines or tissue biopsies.
Asunto(s)
Rastreo Diferencial de Calorimetría/instrumentación , Metabolismo , Adipocitos/metabolismo , Animales , Rastreo Diferencial de Calorimetría/métodos , Diseño de Equipo , Calor , Masculino , Ratones , Microquímica/instrumentación , Mitocondrias Hepáticas/metabolismo , Nanotecnología/instrumentación , Nanotecnología/métodos , Ratas , TransductoresRESUMEN
Developmental changes in the distribution of guanine nucleotide-binding regulatory proteins (G proteins) were investigated in the rat brain during postnatal development. Using a standard or high-resolution urea-SDS-PAGE and specific polyclonal antipeptide antibodies oriented against G(i)alpha1/G(i)alpha2, G(i)alpha3, G(s)alpha, G(o)alpha1/G(o)alpha2, G(q)alpha/G(11)alpha and Gbeta subunit, all these proteins were determined by quantitative immunoblotting in homogenates prepared from cortex, thalamus, hippocampus and pituitary of 1-, 7-, 12-, 18-, 25- and 90-day-old animals. The levels of the majority of G protein alpha subunits, namely G(i)alpha1, G(i)alpha2, G(i)alpha3, G(o)alpha1, G(o)alpha2, G(q)alpha, G(11)alpha and Gbeta, were high already at birth. Whereas the short variant of G(s)alpha, G(s)alphaS, rose sharply in all tested brain regions between postnatal day (PD) 1 and 90, the long variant of G(s)alpha, G(s)alphaL, was unchanged in cortex and thalamus and slightly increased in hippocampus. An increase was observed also in expression of G(i)alpha1/G(i)alpha2 and G(o)alpha1 protein, while G(o)alpha2 remained constant. Minority protein G(o)alpha* dramatically increased in cortex and thalamus, was unchanged in hippocampus and not detectable in pituitary. By contrast, the highest levels of G(i)alpha3 and G(q)alpha/G(11)alpha were detected as early as at PD 1. During the next 90 days, the immunological signal of G(i)alpha3 almost disappeared and G(q)alpha/G(11)alpha continuously declined to the levels corresponding to 50% of the levels determined at birth. Expression of Gbeta subunit was basically unchanged during postnatal development. Our present analysis indicates that G(s)alpha, G(i)alpha/G(o)alpha and G(q)alpha/G(11)alpha proteins are differently expressed in the course of brain development. Differential expression of the individual alpha subunits of trimeric G proteins during postnatal development suggests their different roles in maturation of the brain tissue.
Asunto(s)
Envejecimiento/metabolismo , Encéfalo/crecimiento & desarrollo , Encéfalo/metabolismo , Diferenciación Celular/fisiología , Regulación hacia Abajo/fisiología , Proteínas de Unión al GTP/metabolismo , Neuroglía/metabolismo , Neuronas/metabolismo , Regulación hacia Arriba/fisiología , Animales , Animales Recién Nacidos , Subunidad alfa de la Proteína de Unión al GTP Gi2 , Subunidades alfa de la Proteína de Unión al GTP , Subunidades alfa de la Proteína de Unión al GTP Gi-Go/metabolismo , Subunidades alfa de la Proteína de Unión al GTP Gq-G11 , Subunidades alfa de la Proteína de Unión al GTP Gs/metabolismo , Proteínas de Unión al GTP Heterotriméricas/metabolismo , Masculino , Proteínas Proto-Oncogénicas/metabolismo , Ratas , Ratas WistarRESUMEN
Maturation of the brain adenylyl cyclase (AC) signalling system was investigated in the developing rat cortex, thalamus and hippocampus. Expression of AC type II, IV and VI measured by Western blot dramatically increased in all tested brain regions during the first 3 weeks after birth and these levels were maintained in adulthood. AC type I did not change during ontogenesis. In parallel, AC enzyme activities were determined in order to obtain the functional correlates to the preceding structural (immunoblot) analyses of trimeric G proteins [Ihnatovych et al., Dev. Brain Res. (2002) in press]. Surprisingly, basal, manganese-, fluoride-, forskolin- and GTP-stimulated adenylyl cyclase developed similarly. The relatively low enzyme activities, which were determined at birth, progressively increased (about four times) to a clear maximum around postnatal day PD 12. This was followed by a progressive regression to adulthood so that activity of AC at PD 90 was comparable with the low neonatal level. The peak of AC activities at PD 12 was detected in all tested brain regions. Stimulatory (isoproterenol) effect on basal AC activity as well as inhibitory (baclofen) effect on forskolin-stimulated AC activity were unchanged between PD 12 and PD 90. Thus, comparison of results of the structural and functional analyses of adenylyl cyclase signalling system revealed a clear dissociation between the increase in the amount protein of various AC isoforms and the decrease of total G-protein mediated enzyme activities between PD 12 and adulthood. As none of the complex changes in trimeric G protein levels can explain this difference, the future research has to be oriented to identification of potential negative regulators of AC in the course of brain development. Among these, the newly discovered group of GTPase activating proteins, RGS, appears to be of primary importance because these proteins represent potent negative regulators of any G protein-mediated signalling in brain.