RESUMEN
A role for toll-like receptor 4 (TLR4) has been suggested in previous studies of glomerulonephritis, but the complex integration of these effects has not been explored. To separate effects on the innate and adaptive immune responses, we use the autologous nephrotoxic nephritis model with two disease induction protocols. First, we give a TLR4 ligand at the time of immunization and show the effects are mediated via TLR4 by comparing wild-type and TLR4-deficient mice. In wild-type mice histological measures of disease and serum creatinine are all at least twice as high as TLR4-deficient mice, due to an enhanced immune response to the nephritogenic sheep IgG. Second, we stimulate TLR4 later in the course of disease development and construct four groups of bone marrow chimeric or sham chimeric mice to study the role of TLR4 on bone marrow or renal cells. The most striking finding is that renal cell TLR4 stimulation increases glomerular crescent formation, with a mean of 21% and 25% in the two groups of mice with renal cell TLR4 compared with 0.1% and 0.6% in the two groups without, with differences mirrored by changes in serum creatinine. These findings, in a single disease model, illustrate that TLR4 stimulation triggers crescentic glomerulonephritis by effects on both the adaptive and innate immune response, with a crucial direct effect on renal cells.
Asunto(s)
Células Epiteliales/metabolismo , Glomerulonefritis/inmunología , Glomérulos Renales , Receptor Toll-Like 4/inmunología , Animales , Quimera/inmunología , Células Epiteliales/citología , Células Epiteliales/patología , Glomerulonefritis/patología , Sistema Inmunológico/inmunología , Glomérulos Renales/citología , Glomérulos Renales/patología , Ratones , Ratones Endogámicos C57BL , Receptor Toll-Like 4/genéticaAsunto(s)
Hipercalcemia/prevención & control , Hiperfosfatemia/prevención & control , Fallo Renal Crónico/terapia , Diálisis Renal/métodos , Deficiencia de Vitamina D/etiología , Vitamina D , Vitaminas , Calcio/sangre , Contraindicaciones , Humanos , Hipercalcemia/etiología , Hipercalcemia/metabolismo , Hiperfosfatemia/etiología , Hiperfosfatemia/metabolismo , Fallo Renal Crónico/complicaciones , Fosfatos/sangre , Pronóstico , Vitamina D/uso terapéutico , Deficiencia de Vitamina D/tratamiento farmacológico , Vitaminas/uso terapéuticoRESUMEN
The relative ability of IgG subclasses to cause acute inflammation and the roles of specific effector mechanisms in this process are not clear. We explored this in an in vivo model of glomerular inflammation in the mouse. Trinitrophenol was planted on the glomerular basement membrane after conjugation to nephrotoxic Ab. The relative nephritogenicity of anti-trinitrophenol switch variant mAbs was then explored and shown to be IgG2a > IgG2b, with no disease caused by IgG1. Using knockout mice, we showed that FcgammaRIII was necessary for both neutrophil influx and glomerular damage induced by IgG2a and IgG2b. Surprisingly, IgG1 did not cause disease although it binds to FcgammaRIII. Using blocking Abs, we showed that this was explained by an additional requirement for FcgammaRIV, which does not bind to IgG1. IgG2a- or IgG2b-induced neutrophil influx was not affected by deficiency of either FcgammaRI or C3. Bone marrow chimeras were constructed to test the effect of combined deficiency of FcgammaRI and C3, and there was no effect on IgG2a- or IgG2b-mediated neutrophil influx. However, IgG2b-induced albuminuria and thrombosis were reduced in C3-deficient mice, showing an additional role for complement in IgG2b-mediated glomerular damage. The results show that IgG2a and IgG2b are the pathogenic subclasses in acute neutrophil-mediated glomerular inflammation, with an indispensable role for both FcgammaRIII and FcgammaRIV. Additionally, complement contributes to IgG2b-induced glomerular injury.
Asunto(s)
Anticuerpos Monoclonales/toxicidad , Glomerulonefritis/inmunología , Glomerulonefritis/patología , Cambio de Clase de Inmunoglobulina/genética , Inmunoglobulina G/clasificación , Inmunoglobulina G/toxicidad , Receptores de IgG/fisiología , Enfermedad Aguda , Animales , Anticuerpos Monoclonales/genética , Activación de Complemento/genética , Activación de Complemento/inmunología , Complemento C3/deficiencia , Complemento C3/genética , Glomerulonefritis/metabolismo , Inmunoglobulina G/biosíntesis , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Ratones Noqueados , Picratos/inmunología , Picratos/toxicidad , Proteinuria/inmunología , Proteinuria/patología , Receptores de IgG/deficiencia , Receptores de IgG/genética , Trombosis/inmunología , Trombosis/patologíaRESUMEN
Autoimmune diseases such as glomerulonephritis are exacerbated by infection. This study examined the effect of the Toll-like receptor 4 (TLR4) ligand lipid A on the development of heterologous nephrotoxic nephritis. Administration of nephrotoxic antibody resulted in significant glomerular neutrophil infiltration and albuminuria only when a TLR4 ligand was administered simultaneously. The contribution of TLR4 on renal cells and circulating leukocytes was assessed. Bone marrow chimeras were constructed with TLR4 only on renal cells or bone marrow-derived cells. The administration of nephrotoxic serum and lipid A caused a neutrophil influx in both chimeric groups greater than in sham chimeras that were totally TLR4 deficient but significantly less than in sham chimeras that were totally TLR4 sufficient. Both chimeric groups had greater albuminuria than totally TLR4-deficient sham chimeras; however, the chimeras with TLR4 only on intrinsic renal cells had significantly less than the sham positive group. In situ hybridization showed expression of TLR4 mRNA in mesangial cells and glomerular epithelial cells. For investigation of the potential mechanism by which renal cells could contribute to disease exacerbation, mesangial cells were cultured and found to express mRNA for TLR4, and stimulation of wild-type and TLR4-deficient mesangial cells with LPS caused production of CXC chemokines by wild-type cells only. Treatment of chimeras with TLR4 present only on intrinsic renal cells with anti-CXCL1 and anti-CXCL2 antibody before disease induction significantly reduced renal neutrophil infiltration. These results show that TLR4 on both circulating leukocytes and intrinsic renal cells contributes to the inflammatory effects of antibody deposition within the glomerulus, which depends at least in part on the production of CXC chemokines by intrinsic renal cells.
Asunto(s)
Quimiocina CXCL1/metabolismo , Quimiocina CXCL2/metabolismo , Glomerulonefritis/inmunología , Glomerulonefritis/metabolismo , Células Mesangiales/inmunología , Receptor Toll-Like 4/metabolismo , Albuminuria/inmunología , Albuminuria/metabolismo , Albuminuria/patología , Animales , Células de la Médula Ósea/citología , Células de la Médula Ósea/inmunología , Células de la Médula Ósea/metabolismo , Células Cultivadas , Quimiocina CXCL1/inmunología , Quimiocina CXCL2/inmunología , Femenino , Glomerulonefritis/patología , Inmunoglobulina G/metabolismo , Inmunoglobulina G/farmacología , Ligandos , Lípido A/farmacología , Masculino , Células Mesangiales/citología , Células Mesangiales/metabolismo , Ratones , Ratones Endogámicos C57BL , Ratones Mutantes , Neutrófilos/inmunología , Neutrófilos/patología , Índice de Severidad de la Enfermedad , Ovinos , Receptor Toll-Like 4/genética , Receptor Toll-Like 4/inmunologíaRESUMEN
Infection may exacerbate organ-specific autoimmune disease such as glomerulonephritis. This may occur in the absence of a measurable effect on the adaptive immune response, and the mechanisms responsible are not fully understood. To investigate this, we have studied the effect of TLR2 ligation by the synthetic ligand Pam(3)CysSK(4) on the development of glomerulonephritis in mice. We demonstrated that glomerular inflammation induced by passive administration of nephrotoxic Ab does not occur in the absence of TLR2 stimulation, with a strong synergy when Ab deposition and TLR2 stimulation occur together. Parameters of glomerular inflammation were neutrophil influx, thrombosis, and albuminuria. To investigate the relative contribution of TLR2 on bone marrow-derived cells and intrinsic renal cells, we constructed bone marrow chimeras. Nephrotoxic Ab and TLR2 ligation caused a neutrophil influx in both types of chimera above [corrected] that seen in sham chimeras totally TLR2 deficient [corrected] Albuminuria was seen in both types of chimera above that seen in sham chimeras that were totally TLR2 deficient. This was greater in chimeras with TLR2 present on bone marrow-derived cells. To find a potential mechanism by which intrinsic renal cells may contribute toward disease exacerbation, mesangial cells were studied and shown to express TLR2 and MyD88. Wild-type but not TLR2-deficient mesangial cells produced CXC chemokines in response to stimulation with Pam(3)CysSK(4). These results demonstrate that TLR2 stimulation on both bone marrow-derived and resident tissue cells plays a role in amplifying the inflammatory effects of Ab deposition in the glomerulus.
Asunto(s)
Glomerulonefritis/inmunología , Glomerulonefritis/patología , Glomérulos Renales/inmunología , Glomérulos Renales/patología , Receptor Toll-Like 2/fisiología , Proteínas Adaptadoras Transductoras de Señales/biosíntesis , Albuminuria/inmunología , Albuminuria/metabolismo , Albuminuria/patología , Animales , Células de la Médula Ósea/inmunología , Células de la Médula Ósea/metabolismo , Movimiento Celular/inmunología , Quimiocinas CXC/biosíntesis , Endotoxinas/administración & dosificación , Endotoxinas/sangre , Mesangio Glomerular/inmunología , Mesangio Glomerular/metabolismo , Glomerulonefritis/genética , Glomerulonefritis/metabolismo , Inmunoglobulina G/metabolismo , Inyecciones Intravenosas , Glomérulos Renales/metabolismo , Recuento de Leucocitos , Lipopéptidos , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Factor 88 de Diferenciación Mieloide , Neutrófilos/patología , Péptidos/administración & dosificación , Índice de Severidad de la Enfermedad , Receptor Toll-Like 2/antagonistas & inhibidores , Receptor Toll-Like 2/biosíntesis , Receptor Toll-Like 2/deficienciaRESUMEN
Glomerulonephritis can be exacerbated by infection. This study investigated the effect of N-palmitoyl-S-(2,3-bis(palmitoyloxy)-(2R,S)-propyl)-(R)-cysteinyl-seryl-(lysyl)3-lysine (Pam3CysSK4), a synthetic Toll-like receptor 2 (TLR2) ligand, that was given at immunization on disease severity in accelerated nephrotoxic nephritis. Stimulation of TLR by microbial constituents is known to influence the development of the adaptive immunity. It was hypothesized that the TLR2 ligand Pam3CysSK4 can modulate the development of disease in accelerated nephrotoxic nephritis by influencing the development of adaptive immunity. It is shown that Pam3CysSK4, when given at immunization, can increase profoundly the severity of disease, and with the use of TLR2-deficient mice, it is shown that this disease exacerbation depends on the presence of TLR2. Wild-type mice that were given Pam3CysSK4 at immunization and had more severe disease also had a greater amount of antigen-specific IgG1, IgG2b, and IgG3 in the serum and more IgG2b and IgG3 deposited within the glomerulus. They also had increased numbers of glomerular CD4-positive T cells. Therefore, the more severe disease that was seen in the group that was immunized with lipopeptide can be attributed to an influence on the adaptive immune response.