Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 11 de 11
Filtrar
Más filtros












Base de datos
Intervalo de año de publicación
1.
Quant Plant Biol ; 4: e1, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-37077702

RESUMEN

Plant organ morphogenesis spans several orders of magnitude in time and space. Because of limitations in live-imaging, analysing whole organ growth from initiation to mature stages typically rely on static data sampled from different timepoints and individuals. We introduce a new model-based strategy for dating organs and for reconstructing morphogenetic trajectories over unlimited time windows based on static data. Using this approach, we show that Arabidopsis thaliana leaves are initiated at regular 1-day intervals. Despite contrasted adult morphologies, leaves of different ranks exhibited shared growth dynamics, with linear gradations of growth parameters according to leaf rank. At the sub-organ scale, successive serrations from same or different leaves also followed shared growth dynamics, suggesting that global and local leaf growth patterns are decoupled. Analysing mutants leaves with altered morphology highlighted the decorrelation between adult shapes and morphogenetic trajectories, thus stressing the benefits of our approach in identifying determinants and critical timepoints during organ morphogenesis.

2.
Cell Rep ; 41(12): 111848, 2022 12 20.
Artículo en Inglés | MEDLINE | ID: mdl-36543124

RESUMEN

Cerebral small vessel disease and brain white matter injury are worsened by cardiovascular risk factors including obesity. Molecular pathways in cerebral endothelial cells activated by chronic cerebrovascular risk factors alter cell-cell signaling, blocking endogenous and post-ischemic white matter repair. Using cell-specific translating ribosome affinity purification (RiboTag) in white matter endothelia and oligodendrocyte progenitor cells (OPCs), we identify a coordinated interleukin-chemokine signaling cascade within the oligovascular niche of subcortical white matter that is triggered by diet-induced obesity (DIO). DIO induces interleukin-17B (IL-17B) signaling that acts on the cerebral endothelia through IL-17Rb to increase both circulating and local endothelial expression of CXCL5. In white matter endothelia, CXCL5 promotes the association of OPCs with the vasculature and triggers OPC gene expression programs regulating cell migration through chemokine signaling. Targeted blockade of IL-17B reduced vessel-associated OPCs by reducing endothelial CXCL5 expression. In multiple human cohorts, blood levels of CXCL5 function as a diagnostic and prognostic biomarker of vascular cognitive impairment.


Asunto(s)
Lesiones Encefálicas , Sustancia Blanca , Ratones , Humanos , Animales , Interleucina-17/metabolismo , Sustancia Blanca/metabolismo , Células Endoteliales/metabolismo , Encéfalo/metabolismo , Transducción de Señal , Lesiones Encefálicas/metabolismo , Oligodendroglía/metabolismo , Quimiocina CXCL5/metabolismo
3.
Plant Cell ; 34(12): 4738-4759, 2022 11 29.
Artículo en Inglés | MEDLINE | ID: mdl-36029254

RESUMEN

Stem cells play important roles in animal and plant biology, as they sustain morphogenesis and tissue replenishment following aging or injury. In plants, stem cells are embedded in multicellular structures called meristems. The formation of new meristems is essential for the plastic expansion of the highly branched shoot and root systems. In particular, axillary meristems (AMs) that produce lateral shoots arise from the division of boundary domain cells at the leaf base. The CUP-SHAPED COTYLEDON (CUC) genes are major determinants of the boundary domain and are required for AM initiation. However, how AMs get structured and how stem cells become established de novo remain elusive. Here, we show that two NGATHA-LIKE (NGAL) transcription factors, DEVELOPMENT-RELATED PcG TARGET IN THE APEX4 (DPA4)/NGAL3 and SUPPRESSOR OF DA1-1 7 (SOD7)/NGAL2, redundantly repress CUC expression in initiating AMs of Arabidopsis thaliana. Ectopic boundary fate leads to abnormal growth and organization of the AM and prevents de novo stem cell establishment. Floral meristems of the dpa4 sod7 double mutant show a similar delay in de novo stem cell establishment. Altogether, while boundary fate is required for the initiation of AMs, our work reveals how it is later repressed to allow proper meristem establishment and de novo stem cell niche formation.


Asunto(s)
Proteínas de Arabidopsis , Arabidopsis , Meristema/metabolismo , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Regulación de la Expresión Génica de las Plantas/genética , Arabidopsis/metabolismo , Células Madre/metabolismo , Brotes de la Planta/genética , Factores de Transcripción/metabolismo
4.
Biomedicines ; 10(5)2022 May 12.
Artículo en Inglés | MEDLINE | ID: mdl-35625863

RESUMEN

Food odour is a potent stimulus of food intake. Odour coding in the brain occurs in synergy or competition with other sensory information and internal signals. For eliciting feeding behaviour, food odour coding has to gain signification through enrichment with additional labelling in the brain. Since the ventral striatum, at the crossroads of olfactory and reward pathways, receives a rich dopaminergic innervation, we hypothesized that dopamine plays a role in food odour information processing in the ventral striatum. Using single neurones recordings in anesthetised rats, we show that some ventral striatum neurones respond to food odour. This neuronal network displays a variety of responses (excitation, inhibition, rhythmic activity in phase with respiration). The localization of recorded neurones in a 3-dimensional brain model suggests the spatial segregation of this food-odour responsive population. Using local field potentials recordings, we found that the neural population response to food odour was characterized by an increase of power in the beta-band frequency. This response was modulated by dopamine, as evidenced by its depression following administration of the dopaminergic D1 and D2 antagonists SCH23390 and raclopride. Our results suggest that dopamine improves food odour processing in the ventral striatum.

5.
Mol Metab ; 36: 100963, 2020 06.
Artículo en Inglés | MEDLINE | ID: mdl-32283518

RESUMEN

OBJECTIVE: Maternal unbalanced nutritional habits during embryonic development and perinatal stages perturb hypothalamic neuronal programming of the offspring, thus increasing obesity-associated diabetes risk. However, the underlying molecular mechanisms remain largely unknown. In this study we sought to determine the translatomic signatures associated with pro-opiomelanocortin (POMC) neuron malprogramming in maternal obesogenic conditions. METHODS: We used the RiboTag mouse model to specifically profile the translatome of POMC neurons during neonatal (P0) and perinatal (P21) life and its neuroanatomical, functional, and physiological consequences. RESULTS: Maternal high-fat diet (HFD) exposure did not interfere with offspring's hypothalamic POMC neuron specification, but significantly impaired their spatial distribution and axonal extension to target areas. Importantly, we established POMC neuron-specific translatome signatures accounting for aberrant neuronal development and axonal growth. These anatomical and molecular alterations caused metabolic dysfunction in early life and adulthood. CONCLUSIONS: Our study provides fundamental insights on the molecular mechanisms underlying POMC neuron malprogramming in obesogenic contexts.


Asunto(s)
Obesidad/genética , Efectos Tardíos de la Exposición Prenatal/metabolismo , Proopiomelanocortina/metabolismo , Animales , ADN/genética , Metilación de ADN , Dieta Alta en Grasa , Femenino , Estudio de Asociación del Genoma Completo , Hipotálamo/metabolismo , Masculino , Ratones , Neurogénesis/genética , Neuronas/metabolismo , Obesidad/metabolismo , Embarazo/genética , Embarazo/metabolismo , Proopiomelanocortina/fisiología
6.
J Physiol ; 597(13): 3407-3423, 2019 07.
Artículo en Inglés | MEDLINE | ID: mdl-31077360

RESUMEN

KEY POINTS: Spinally-projecting neurons of the rostral ventrolateral medulla (RVLM) determine sympathetic outflow to different territories of the body. Previous studies suggest the existence of RVLM neurons with distinct functional classes, such as neurons that target sympathetic nerves bound for functionally-similar tissue types (e.g. muscle vasculature). The existence of RVLM neurons with more general actions had not been critically tested. Using viral tracing, we show that a significant minority of RVLM neurons send axon collaterals to disparate spinal segments (T2 and T10 ). Furthermore, optogenetic activation of sympathetic premotor neurons projecting to lumbar spinal segments also produced activation of sympathetic nerves from rostral spinal segments that innervate functionally diverse tissues (heart and forelimb muscle). These findings suggest the existence of individual RVLM neurons for which the axons branch to drive sympathetic preganglionic neurons of more than one functional class and may be able to produce global changes in sympathetic activity. ABSTRACT: We investigate the extent of spinal axon collateralization of rat rostral ventrolateral medulla (RVLM) sympathetic premotor neurons and its functional consequences. In anatomical tracing experiments, two recombinant herpes viral vectors with retrograde tropism and expressing different fluorophores were injected into the intermediolateral column at upper thoracic and lower thoracic levels. Histological analysis revealed that ∼21% of RVLM bulbospinal neurons were retrogradely labelled by both vectors, indicating substantial axonal collateralization to disparate spinal segments. In functional experiments, another virus with retrograde tropism, a canine adenovirus expressing Cre recombinase, was injected into the left intermediolateral horn around the thoracolumbar junction, whereas a Cre-dependent viral vector encoding Channelrhodopsin2 under LoxP control was injected into the ipsilateral RVLM. In subsequent terminal experiments, blue laser light (473 nm × 20 ms pulses at 10 mW) was used to activate RVLM neurons that had been transduced by both vectors. Stimulus-locked activation, at appropriate latencies, was recorded in the following pairs of sympathetic nerves: forelimb and hindlimb muscle sympathetic fibres, as well as cardiac and either hindlimb muscle or lumbar sympathetic nerves. The latter result demonstrates that axon collaterals of lumbar-projecting RVLM neurons project to, and excite, both functionally similar (forelimb and hindlimb muscle) and functionally dissimilar (lumbar and cardiac) preganglionic neurons. Taken together, these findings show that the axons of a significant proportion of RVLM neurons collateralise widely within the spinal cord, and that they may excite preganglionic neurons of more than one functional class.


Asunto(s)
Axones/fisiología , Neuronas/fisiología , Médula Espinal/fisiología , Sistema Nervioso Simpático/fisiología , Animales , Fibras Autónomas Preganglionares/fisiología , Miembro Posterior/fisiología , Interneuronas/fisiología , Masculino , Bulbo Raquídeo/fisiología , Músculos/fisiología , Vías Nerviosas/fisiología , Ratas , Ratas Sprague-Dawley
7.
Development ; 143(18): 3417-28, 2016 09 15.
Artículo en Inglés | MEDLINE | ID: mdl-27387872

RESUMEN

A major challenge in morphometrics is to analyse complex biological shapes formed by structures at different scales. Leaves exemplify this challenge as they combine differences in their overall shape with smaller shape variations at their margin, leading to lobes or teeth. Current methods based on contour or on landmark analysis are successful in quantifying either overall leaf shape or leaf margin dissection, but fail in combining the two. Here, we present a comprehensive strategy and its associated freely available platform for the quantitative, multiscale analysis of the morphology of leaves with different architectures. For this, biologically relevant landmarks are automatically extracted and hierarchised, and used to guide the reconstruction of accurate average contours that properly represent both global and local features. Using this method, we establish a quantitative framework of the developmental trajectory of Arabidopsis leaves of different ranks and retrace the origin of leaf heteroblasty. When applied to different mutant forms, our method can contribute to a better understanding of gene function, as we show here for the role of CUC2 during Arabidopsis leaf serration. Finally, we illustrate the wider applicability of our tool by analysing hand morphometrics.


Asunto(s)
Hojas de la Planta/anatomía & histología , Programas Informáticos , Arabidopsis/anatomía & histología , Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Microscopía Fluorescente , Hojas de la Planta/metabolismo
8.
Plant J ; 87(2): 230-42, 2016 07.
Artículo en Inglés | MEDLINE | ID: mdl-27121260

RESUMEN

The localization of proteins in specific domains or compartments in the 3D cellular space is essential for many fundamental processes in eukaryotic cells. Deciphering spatial organization principles within cells is a challenging task, in particular because of the large morphological variations between individual cells. We present here an approach for normalizing variations in cell morphology and for statistically analyzing spatial distributions of intracellular compartments from collections of 3D images. The method relies on the processing and analysis of 3D geometrical models that are generated from image stacks and that are used to build representations at progressively increasing levels of integration, ultimately revealing statistical significant traits of spatial distributions. To make this methodology widely available to end-users, we implemented our algorithmic pipeline into a user-friendly, multi-platform, and freely available software. To validate our approach, we generated 3D statistical maps of endomembrane compartments at subcellular resolution within an average epidermal root cell from collections of image stacks. This revealed unsuspected polar distribution patterns of organelles that were not detectable in individual images. By reversing the classical 'measure-then-average' paradigm, one major benefit of the proposed strategy is the production and display of statistical 3D representations of spatial organizations, thus fully preserving the spatial dimension of image data and at the same time allowing their integration over individual observations. The approach and software are generic and should be of general interest for experimental and modeling studies of spatial organizations at multiple scales (subcellular, cellular, tissular) in biological systems.


Asunto(s)
Células/ultraestructura , Imagenología Tridimensional/métodos , Arabidopsis/ultraestructura , Proteínas Fluorescentes Verdes/metabolismo , Programas Informáticos , Análisis Espacial , Fracciones Subcelulares/ultraestructura
9.
PLoS One ; 9(2): e87759, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-24505311

RESUMEN

In biological systems, functions and spatial organizations are closely related. Spatial data in biology frequently consist of, or can be assimilated to, sets of points. An important goal in the quantitative analysis of such data is the evaluation and localization of differences in spatial distributions between groups. Because of experimental replications, achieving this goal requires comparing collections of point sets, a noticeably challenging issue for which no method has been proposed to date. We introduce a strategy to address this problem, based on the comparison of point intensities throughout space. Our method is based on a statistical test that determines whether local point intensities, estimated using replicated data, are significantly different or not. Repeating this test at different positions provides an intensity comparison map and reveals domains showing significant intensity differences. Simulated data were used to characterize and validate this approach. The method was then applied to two different neuroanatomical systems to evaluate its ability to reveal spatial differences in biological data sets. Applied to two distinct neuronal populations within the rat spinal cord, the method generated an objective representation of the spatial segregation established previously on a subjective visual basis. The method was also applied to analyze the spatial distribution of locus coeruleus neurons in control and mutant mice. The results objectively consolidated previous conclusions obtained from visual comparisons. Remarkably, they also provided new insights into the maturation of the locus coeruleus in mutant and control animals. Overall, the method introduced here is a new contribution to the quantitative analysis of biological organizations that provides meaningful spatial representations which are easy to understand and to interpret. Finally, because our approach is generic and punctual structures are widespread at the cellular and histological scales, it is potentially useful for a large spectrum of applications for the analysis of biological systems.


Asunto(s)
Locus Coeruleus/citología , Modelos Teóricos , Neuroimagen/métodos , Neuronas/citología , Reconocimiento de Normas Patrones Automatizadas/métodos , Médula Espinal/citología , Animales , Ratones , Ratones Mutantes , Ratas
10.
PLoS One ; 5(2): e8974, 2010 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-20126542

RESUMEN

BACKGROUND: The caudal brainstem plays an important role in short-term satiation and in the control of meal termination. Meal-related stimuli sensed by the gastrointestinal (GI) tract are transmitted to the area postrema (AP) via the bloodstream, or to the nucleus tractus solitarii (NTS) via the vagus nerve. Little is known about the encoding of macronutrient-specific signals in the caudal brainstem. We hypothesized that sucrose and casein peptone activate spatially distinct sub-populations of NTS neurons and thus characterized the latter using statistical three-dimensional modeling. METHODOLOGY/PRINCIPAL FINDINGS: Using immunolabeling of the proto-oncogene Fos as a marker of neuronal activity, in combination with a statistical three-dimensional modeling approach, we have shown that NTS neurons activated by sucrose or peptone gavage occupy distinct, although partially overlapping, positions. Specifically, when compared to their homologues in peptone-treated mice, three-dimensional models calculated from neuronal density maps following sucrose gavage showed that Fos-positive neurons occupy a more lateral position at the rostral end of the NTS, and a more dorsal position at the caudal end. CONCLUSION/SIGNIFICANCE: To our knowledge, this is the first time that subpopulations of NTS neurons have be distinguished according to the spatial organization of their functional response. Such neuronal activity patterns may be of particular relevance to understanding the mechanisms that support the central encoding of signals related to the presence of macronutrients in the GI tract during digestion. Finally, this finding also illustrates the usefulness of statistical three-dimensional modeling to functional neuroanatomical studies.


Asunto(s)
Tronco Encefálico/anatomía & histología , Tronco Encefálico/metabolismo , Modelos Anatómicos , Proteínas Proto-Oncogénicas c-fos/biosíntesis , Animales , Tronco Encefálico/citología , Caseínas/administración & dosificación , Proteínas en la Dieta/administración & dosificación , Sacarosa en la Dieta/administración & dosificación , Inmunohistoquímica , Masculino , Ratones , Ratones Endogámicos C57BL , Neuronas/citología , Neuronas/efectos de los fármacos , Neuronas/metabolismo , Núcleo Solitario/anatomía & histología , Núcleo Solitario/citología , Núcleo Solitario/metabolismo , Sacarosa/administración & dosificación
11.
J Comp Neurol ; 513(5): 483-95, 2009 Apr 10.
Artículo en Inglés | MEDLINE | ID: mdl-19226531

RESUMEN

An algorithm for the three-dimensional statistical representation of neuronal populations was designed and implemented. Using this algorithm a series of 3D models, calculated from repeated histological experiments, can be combined to provide a synthetic vision of a population of neurons taking into account biological and experimental variability. Based on the point process theory, our algorithm allows computation of neuronal density maps from which isodensity surfaces can be readily extracted and visualized as surface models revealing the statistical organization of the neuronal population under study. This algorithm was applied to the spatial distribution of locus coeruleus (LC) neurons of 30- and 90-day-old control and quaking mice. By combining 12 3D models of the LC, a region of the nucleus in which a subpopulation of neurons loses its noradrenergic phenotype between 30 and 90 days postnatally was demonstrated in control mice but not in quaking mice, leading to the hyperplasia previously reported in adult mutants. Altogether, this algorithm allows computation of 3D statistical and graphical models of neuronal populations, providing a contribution to quantitative 3D neuroanatomical modeling.


Asunto(s)
Algoritmos , Imagenología Tridimensional/métodos , Locus Coeruleus/anatomía & histología , Neuronas/metabolismo , Tirosina 3-Monooxigenasa/metabolismo , Factores de Edad , Animales , Recuento de Células , Locus Coeruleus/citología , Locus Coeruleus/enzimología , Ratones , Ratones Quaking , Modelos Estadísticos
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA
...