Protein redox by a piezoelectric acousto-nanodevice.

Protein redox is responsible for many crucial biological processes; thus, the ability to modulate the redox proteins through external stimuli presents a unique opportunity to tune the system. In this work, we present an acousto-nanodevice that is capable of oxidizing redox protein under ultrasonic irradiation via surface-engineered barium titanate (BTO) nanoparticles with a gold half-coating. Using cytochrome c as the model protein, we demonstrate nanodevice-mediated protein oxidation. BINased on our experimental observations, we reveal that the electron transfer occurs in one direction due to the alternating electrical polarization of BTO under ultrasound. Such unique unidirectional electron transfer is enabled by modulating the work function of the gold surface with respect to the redox center. The new class of ultrasonically powered nano-sized protein redox agents could be a modulator for biological processes with high selectivity and deeper treatment sites.

Light responsive plasmonic silicone elastomer/hydrogel soft actuator.

Soft robots offer unique advantages in their ability to interact with fragile organisms. Light responsive soft actuators are promising for the development of the untethered soft robots. However, this requires a mechanism for converting the externally applied light into mechanical actuation. We designed, fabricated, and tested a light responsive soft actuator consist of silicone elastomer with plasmonic metal nanoparticle embedment and temperature sensitive hydrogel. We found that the selection of the carrier solvent for the metal nanoparticle embedment in silicone elastomer to be crucial. The fabricated soft actuator showed a relatively fast response time (< 5 min) under light illumination.

Implantable Cisplatin Synthesis Microdevice for Regional Chemotherapy.

Cisplatin, the first platinum chemotherapy agent to obtain Food and Drug Administration (FDA) approval in 1978, is widely used for a number of cancers. However, the painful side effects stemming from systemic delivery are the inevitable limitation of cisplatin. A possible solution is regional chemotherapy using various drug delivery systems, which reduces the systemic toxicity and increases drug accumulation in the tumor. In this paper, a rice-grain sized, ultrasonically powered, and implantable microdevice that can synthesize cisplatin in situ is presented. The microdevice produces 0.7 mg of cisplatin within 1 h under ultrasonic irradiation (400 mW cm-2 ). The effect of the microdevice-synthesized cisplatin is evaluated using in vitro murine breast cancer cells and ex vivo liver tissue. The results suggest that cytotoxic activities of the microdevice-mediated cisplatin delivery are significantly higher in both in vitro and ex vivo experiments. Overall, the proposed cisplatin synthesis microdevice represents a strong alternative treatment option for regional chemotherapy.

A Hydrogel-Based Ultrasonic Backscattering Wireless Biochemical Sensing.

Wireless monitoring of the physio-biochemical information is becoming increasingly important for healthcare. In this work, we present a proof-of-concept hydrogel-based wireless biochemical sensing scheme utilizing ultrasound. The sensing system utilizes silica-nanoparticle embedded hydrogel deposited on a thin glass substrate, which presents two prominent interfaces for ultrasonic backscattering (tissue/glass and hydrogel/glass). To overcome the effect of the varying acoustic properties of the intervening biological tissues between the sensor and the external transducer, we implemented a differential mode of ultrasonic back-scattering. Here, we demonstrate a wireless pH measurement with a resolution of 0.2 pH level change and a wireless sensing range around 10 cm in a water tank.

Ultrasonic Hydrogel Biochemical Sensor System.

In this work, we present a proof-of-concept hydrogel-based sensor system capable of wireless biochemical sensing through measuring backscattered ultrasound. The system consists of silica-nanoparticle embedded hydrogel deposited on a thin glass substrate, presenting two interfaces for backscattering (tissue/hydrogel and hydrogel/glass), which allows for system output to be invariant under the change in acoustic properties (e.g. attenuation, reflection) of the intervening biological tissue. We characterize the effect of silica nanoparticles (acoustic contrast agents) loading on the hydrogel's swelling ratio and its ultrasonic backscattering properties. We demonstrate a wireless pH measurement using dual modes of interrogations, reflection ratio and time delay. The ultrasonic hydrogel pH sensor is demonstrated with a sensing resolution of 0.2 pH level change with a wireless sensing distance around 10 cm.

Untethered Actuation of Hybrid Hydrogel Gripper via Ultrasound.

Stimuli-responsive hydrogels that exhibit reversible volume changes in response to stimulus cues such as heat, pH, and light have been utilized in soft robotics, microfluidics, electronics, and biomedical surgical tools. While the development of the soft robotics has widely expanded, most external triggering systems still have limited utilities due to the low selectivity. We present a hybrid gripper capable of undergoing preprogrammed shape transformation utilizing ultrasound energy on-off processes as the external triggering system, which can be utilized in invisible and nonselective environments. Furthermore, we describe the magnetic locomotion of the soft gripper enabled by the introduction of iron oxide (Fe2O3) ferrogel. By integrating these dual ultrasonic and magnetic control systems, we demonstrate the soft gripper could actively and safely perform pick-and-place tasks on a biological salmon roe in the aqueous maze environment. We expect that this study can provide the groundwork for the further important advances to the creation of ultrasound-responsive shape programmable and multifunctional smart soft robots.