RESUMEN
Objective. To optimise the use of freeze/thaw testicular immotile spermatozoa from nonobstructive azoospermia patients and to analyse the outcome of intracytoplasmic sperm injection (ICSI) of such spermatozoa. Methods. Testicular specimens were retrieved and cryopreserved from forty patients with nonobstructive azoospermia and underwent one cycle with thawed spermatozoa (Group I) that led to pregnancy in sixteen cases. Twenty-four patients of group I underwent treatment with the same batch of thawed spermatozoa (Group II). For the first ICSI attempt, injection was performed when motile spermatozoa were found. In group II, injection was performed when maximum motility was reached. We compared mean of fertilization rate, embryo quality, clinical pregnancy rate and embryo implantation rate. Results. The mean percentage of motility was significantly higher in the group II than in the group I (18, 6 versus 8, 2). Group I showed a significant decrease in fertilization rates when compared with cryopreserved testicular spermatozoa in group II (54% versus 72%, P < 0.05). No difference was noted between the cleavage rate, embryo quality, clinical pregnancy rates and implantation rates among group II and I. Conclusion. Fecundation rate can be significantly improved after in-vitro culture and sperm selection of frozen-thawed immotile testicular spermatozoa in patients with nonobstructive azoospermia.
Asunto(s)
Fructosa/fisiología , Semen/fisiología , Motilidad Espermática , Cuello del Útero/fisiología , Femenino , Humanos , MasculinoAsunto(s)
Calcio/fisiología , Magnesio/fisiología , Semen/fisiología , Zinc/fisiología , Humanos , MasculinoRESUMEN
An electron-optical study on the endometrium of 5 controls and 25 patients subjected during 3-12 months to 0.25 mg norgestrel + 0.05 mg etinil-estradiol association treatment is performed. The most prominent electronmicroscopical features of endometrial changes are described, such as the asynchronical maturation of the epithelium and the stroma, the shorthening of the proliferative and secretory phases and the epithelial involution towards the last days of the cycle. Special emphasis is placed on the zonal distribution of these changes. An increase of the nostocitosis phenomena is reported and its significance as a complementary contraceptive mechanism is discussed.
PIP: The results of electron microscope studies on the endometrium of 30 women are reported. 25 took a dose of .25 mg norgestrel and .05 mg ethinyl estradiol as an oral contraceptive for a minimum of 3 months and a maximum of 12; the other 5 served as controls. Examinations were made on the 5th, 10th, 19th, and 25th days of the cycle. The authors intended to establish the electron microscopic basis of changes in the endometrium occurring as a result of taking oral contraceptives, to discuss the findings of others in light of their results, and to discuss the possible role of endometrial modifications on the contraceptive effe ct of the drugs. The most prominent endometrial changes are described, such as the asynchronal maturation of the epithelium and the stroma, the shortening of the proliferative and secretory phases and the epithelial involution toward the last days of the cycle. Special emphasis is placed on the zonal distribution of these changes. An increase in the nostocitosis phenomena is reported and its significance as a complementary contraceptive mechanism discussed.