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1.
J Ethnopharmacol ; 224: 1-14, 2018 Oct 05.
Artículo en Inglés | MEDLINE | ID: mdl-29654829

RESUMEN

ETHNOPHARMACOLOGY RELEVANCE: Based on basic theories of Chinese medicine, Yi-Qi-Yang-Yin-Hua-Tan-Qu-Yu (YQYYHTQY) recipe was constituted by eleven kinds of Chinese herbs and effective in treatment of type 2 diabetes (T2DM). But the therapy target was unclear. OBJECTIVE: In this study, we used the serum proteome labeled by iTRAQ to find therapy target of YQYYHTQY recipe on T2DM. MATERIALS AND METHODS: The rat model was induced by high-fat diet (HFD) and streptozotocin (STZ, 30 mg/kg). Drugs were administered to rats once daily for 14 days. Related laboratory parameters were observed. Serum proteome were compared between T2DM and YQYYHTQY group using the iTRAQ labeling quantitative proteomics technique. Functional differential proteins were analysis by STRING software. Target proteins were confirmed by ELISA kits. RESULTS: Hyperglycemia, hyperinsulinemia, insulin resistance, decrease of glucose transporter, depilation, less activity, flock together, depression, ecchymosis of tongue and tail appearance, the typical diabetic patients "a little more than three" symptoms, as well as the decrease of grip strength, serum cyclic adenosine monophosphate (cAMP)/ cyclic guanosine monophosphate (cGMP) ratio, serum high density lipoprotein-cholesterol (HDL-C) and the increase of serum triglyceride (TG), total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C), thromboxane B2 (TXB2)/ 6-keto prostaglandin F1α (6-keto PGF1α) ratio, endothelin-1 (ET-1) levels were found in T2DM group. After drugs treatment, all the above indexes almost were improved in different degrees and effect of YQYYHTQY recipe was superior to pioglitazone hydrochloride. In addition, there were 23 differential proteins, 5 up-regulated and 18 down-regulated proteins. Of them, there were 4 proteins related with diabetes, blood and behavior. Cell division control protein 42 homolog (CDC42) and Ras homolog gene family member A (RhoA) were the therapy targets of YQYYHTQY recipe on T2DM. CONCLUSIONS: YQYYHTQY recipe showed therapy effect on T2DM. CDC42 and RhoA proteins were the therapy targets of YQYYHTQY recipe.


Asunto(s)
Diabetes Mellitus Experimental/sangre , Diabetes Mellitus Tipo 2/sangre , Medicamentos Herbarios Chinos/farmacología , Hipoglucemiantes/farmacología , Proteoma , Animales , Conducta Animal/efectos de los fármacos , Proteínas Sanguíneas/análisis , Diabetes Mellitus Experimental/tratamiento farmacológico , Diabetes Mellitus Tipo 2/tratamiento farmacológico , Medicamentos Herbarios Chinos/uso terapéutico , Hipoglucemiantes/uso terapéutico , Masculino , Proteómica , Ratas Sprague-Dawley
2.
Molecules ; 22(5)2017 Apr 26.
Artículo en Inglés | MEDLINE | ID: mdl-28445422

RESUMEN

Qingwen Baidu Decoction (QBD) is an extraordinarily "cold" formula. It was traditionally used to cure epidemic hemorrhagic fever, intestinal typhoid fever, influenza, sepsis and so on. The purpose of this study was to discover relationships between the change of the constituents in different extracts of QBD and the pharmacological effect in a rat model of acute lung injury (ALI) induced by lipopolysaccharide (LPS). The study aimed to discover the changes in constituents of different QBD extracts and the pharmacological effects on acute lung injury (ALI) induced by LPS. The results demonstrated that high dose and middle dose of QBD had significantly potent anti-inflammatory effects and reduced pulmonary edema caused by ALI in rats (p < 0.05). To explore the underlying constituents of QBD, we assessed its influence of six different QBD extracts on ALI and analyzed the different constituents in the corresponding HPLC chromatograms by a Principal Component Analysis (PCA) method. The results showed that the pharmacological effect of QBD was related to the polarity of its extracts, and the medium polarity extracts E2 and E5 in particular displayed much better protective effects against ALI than other groups. Moreover, HPLC-DAD-ESI-MSn and PCA analysis showed that verbascoside and angoroside C played a key role in reducing pulmonary edema. In addition, the current study revealed that ethyl gallate, pentagalloylglucose, galloyl paeoniflorin, mudanpioside C and harpagoside can treat ALI mainly by reducing the total cells and infiltration of activated polymorphonuclear leukocytes (PMNs).


Asunto(s)
Lesión Pulmonar Aguda/tratamiento farmacológico , Antiinflamatorios/farmacología , Medicamentos Herbarios Chinos/farmacología , Lesión Pulmonar Aguda/inmunología , Animales , Antiinflamatorios/química , Antiinflamatorios/aislamiento & purificación , Medicamentos Herbarios Chinos/análisis , Medicamentos Herbarios Chinos/química , Lipopolisacáridos/farmacología , Pulmón/efectos de los fármacos , Pulmón/inmunología , Pulmón/patología , Masculino , Infiltración Neutrófila/efectos de los fármacos , Ratas Sprague-Dawley , Factor de Necrosis Tumoral alfa/metabolismo
3.
ScientificWorldJournal ; 2015: 291680, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-26495421

RESUMEN

Due to the proved clinical efficacy, Shuang-Huang-Lian (SHL) has developed a variety of dosage forms. However, the in-depth research on targets and pharmacological mechanisms of SHL preparations was scarce. In the presented study, the bioinformatics approaches were adopted to integrate relevant data and biological information. As a result, a PPI network was built and the common topological parameters were characterized. The results suggested that the PPI network of SHL exhibited a scale-free property and modular architecture. The drug target network of SHL was structured with 21 functional modules. According to certain modules and pharmacological effects distribution, an antitumor effect and potential drug targets were predicted. A biological network which contained 26 subnetworks was constructed to elucidate the antipneumonia mechanism of SHL. We also extracted the subnetwork to explicitly display the pathway where one effective component acts on the pneumonia related targets. In conclusions, a bioinformatics approach was established for exploring the drug targets, pharmacological activity distribution, effective components of SHL, and its mechanism of antipneumonia. Above all, we identified the effective components and disclosed the mechanism of SHL from the view of system.


Asunto(s)
Biología Computacional/métodos , Medicamentos Herbarios Chinos/farmacología , Medicamentos Herbarios Chinos/uso terapéutico , Flavonas/farmacología , Flavonoides/farmacología , Glucósidos/farmacología , Humanos , Redes y Vías Metabólicas/efectos de los fármacos , Neumonía/tratamiento farmacológico , Mapas de Interacción de Proteínas/efectos de los fármacos
4.
Zhongguo Zhong Yao Za Zhi ; 39(7): 1229-33, 2014 Apr.
Artículo en Chino | MEDLINE | ID: mdl-25011259

RESUMEN

OBJECTIVE: The aim of the present study was to prepare uniform-sized silybin loaded poly (lactic-co-glycolic acid) (PLGA) microspheres in study of silybin with stainless steel membrane. METHOD: Silybin PLGA microspheres were prepared by stainless steel membrane emulsification. The preparation conditions were optimized by single-factor test and orthogonal experiment, and evaluating the mean diameters, the particle size distribution, drug loading, entrapment efficiency and morphology of microsphere. RESULT: Prepared microspheres were round and surface was smooth. The mean diameter was (4.961 +/- 0.56) microm. The span was (1.75 +/- 0.18). The entrapment efficiency was (54.997 +/- 4.05)% and the average drug loading was (23.6 +/- 1.70)%. CONCLUSION: The stainless steel membrane emulsification can be used to prepare the silybin PLGA microspheres. The mean diameters of the silybin PLGA microspheres can be controlled in certain level. Stainless steel membrane emulsification has great potentiality exploitation and utilization.


Asunto(s)
Composición de Medicamentos/métodos , Ácido Láctico/química , Ácido Poliglicólico/química , Silimarina/química , Emulsiones/química , Microesferas , Tamaño de la Partícula , Copolímero de Ácido Poliláctico-Ácido Poliglicólico , Silibina , Acero Inoxidable/química
5.
Zhongguo Zhong Yao Za Zhi ; 39(22): 4389-93, 2014 Nov.
Artículo en Chino | MEDLINE | ID: mdl-25850273

RESUMEN

This research is to study the relationship between HPLC fingerprints of Moutan Cortex, Paeoniae Radix Rubra and Paeoniae Radix Alba and their activity on lipopolysaccharide-induced acute lung injury. HPLC fingerprints of each extract of Moutan Cortex,Paeoniae Radix Rubra and Paeoniae Radix Alba were established by an optimized HPLC-MS method. The activities of all samples against protein and tumor necrosis a factor were tested by the model of lipopolysaccharide-induced acute lung injury. The possible relationship between HPLC-MS fingerprints and the activitieswere deduced by the Partial least squares regression analysis method. Samples were analyzed by HPLC-MS/MS to identify the major peaks. The results showed that each sample had some effect on acute lung injury. Four components with a lager contribution rate of efficacy were calculated by the research of spectrum-effect relationship. Moutan Cortex exhibited good activity on acute lung injury, and gallic acid, paeoniflorin, galloylpaeoniflorin and paeonol were the main effective components.


Asunto(s)
Lesión Pulmonar Aguda/inducido químicamente , Lesión Pulmonar Aguda/tratamiento farmacológico , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/farmacología , Lipopolisacáridos/farmacología , Paeonia/química , Acetofenonas/química , Acetofenonas/farmacología , Animales , Compuestos Bicíclicos Heterocíclicos con Puentes/química , Compuestos Bicíclicos Heterocíclicos con Puentes/farmacología , Cromatografía Líquida de Alta Presión/métodos , Ácido Gálico/química , Ácido Gálico/farmacología , Glucósidos/química , Glucósidos/farmacología , Masculino , Monoterpenos/química , Monoterpenos/farmacología , Raíces de Plantas/química , Ratas , Ratas Wistar , Espectrometría de Masas en Tándem/métodos
6.
J Asian Nat Prod Res ; 15(8): 849-54, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-23796188

RESUMEN

Phytochemical investigation of the leaves of Aralia elata has led to the isolation of two new compounds 3-O-ß-D-glucopyranosyl-(1 → 3)-ß-D-glucopyranosyl-(1 → 3)-ß-D-glucopyranosyl echinocystic acid (1) and 3-O-ß-D-glucopyranosyl-(1 → 3)-ß-D-glucopyranosyl-(1 → 3)-ß-D-glucopyranosyl oleanolic acid 28-O-ß-D-glucopyranoside (2), together with four known compounds 3-6, which were isolated for the first time from this genus. Structural determination was accomplished by spectroscopic analysis, particularly by ¹³C NMR, 2D NMR, and HR-ESI-MS techniques.


Asunto(s)
Aralia/química , Medicamentos Herbarios Chinos/aislamiento & purificación , Saponinas/aislamiento & purificación , Triterpenos/aislamiento & purificación , Medicamentos Herbarios Chinos/química , Espectroscopía de Resonancia por Spin del Electrón , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Hojas de la Planta/química , Saponinas/química , Estereoisomerismo , Triterpenos/química
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