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Urban green spaces are the soil component in cities that interacts most closely with humans. This study investigated the residues of seven neonicotinoids (NEOs) in soils from urban green spaces within the Pearl River Delta (PRD) region and analyzed the correlation between the residue characteristics and the region's economic development. Notably, we introduced the Nemerow Index method, a comprehensive approach, to quantify the overall pollution level of NEOs in the soil of urban park green spaces and utilized this to assess the cumulative exposure probability risks for different populations in this scenario. We found that: (1) The soil of urban park green spaces exhibited varying degrees of NEOs contamination (Σ7NEOs: N.D.-137.31; 6.25 µg/kg), with imidacloprid and clothianidin constituting the highest proportions (89.46 % and 83.60 %); (2) The residual levels of NEOs in Children's Park were significantly higher than those in community parks within Guangzhou, with an average value of 13.30 µg/kg compared to 3.30 µg/kg; (3) The residual characteristics of NEOs exhibited a positive correlation with regional economic development; specifically, the per capita GDP well correlated with IMIRPF, a summation of seven NEOs in imidacloprid equivalents via relative potency factors (R2 =0.86). Regions with higher economic development typically exhibited elevated IMIRPF levels; (4) The fitted cumulative probability distributions for average daily exposure doses revealed that children's exposure was an order of magnitude higher than adults'. Despite this, the exposure risks for both groups remained within acceptable limits.
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In order to explore the pollution characteristicsï¼ ecological risksï¼ and pollution sources of heavy metals in farmland soils around typical factories in Hunan Provinceï¼ the content characteristics of eight heavy metals in farmland soils around fluoride factoriesï¼ leather factoriesï¼ and plating plants were analyzed. The geo-accumulation index and potential ecological risk index were used to evaluate the pollution and environmental risk of heavy metals. The correlation analysisï¼ hierarchical cluster analysisï¼ and principal component analysis were used to analyze the sources of heavy metals. The Monte-Carlo model was used to evaluate the probability risk of regional ecological risk. The results showed that the main pollution elements in the soil were Cd and Znï¼ and their mean values were 4.46 and 2.73 times the background valuesï¼ respectively. Zn was at a mild pollution level in the soil of the three typical factoriesï¼ and Cd was at a moderate pollution level in the whole fluoride factory. The pollution sources of heavy metals in the typical factories were mainly natural sourcesï¼ industrial activity sources ï¼industrial waste dischargeï¼ mineral miningï¼ and smelting activitiesï¼ï¼ traffic sourcesï¼ etc. The results of potential ecological risk assessment showed that the ecological risk of the fluoride factory was at a high risk levelï¼ and the ecological risk of the leather factory and plating plants was at a high risk level. Cd was the main contributing element. The results of Monte-Carlo probabilistic ecological risk assessment reduced the uncertainty of deterministic assessmentï¼ which could provide scientific basis for accurate risk management and control in the regions.
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The goal of this study is to assess the efficacy and safety of Bifidobacterium animalis subsp. lactis BLa80, as an adjunct treatment for diarrhea in children with a randomized, double-blinded, placebo-controlled study design. Eligible diarrheal children, aged 0-3 years without the need for antibiotic treatment based on clinical diagnosis when recruited, were randomized into the intervention group (IG, n = 58, with probiotic) or the control group (CG, n = 53, placebo). The primary assessment was the duration of diarrhea. Fecal samples were collected for biochemical index measurement, analysis of gut microbiome composition, and prediction of gene family abundances. The total duration of diarrhea in the IG (122.6 ± 13.1 h) was significantly shorter than in the CG (148.4 ± 17.6 h, p < 0.001). More children in the IG showed improvements in diarrhea compared to the CG, both in intention-to-treat analysis (81.7% vs. 40.0%, p < 0.001) and per protocol analysis (84.4% vs 45.3%, p < 0.001). Cathelicidin level in the IG was significantly higher than that in the CG after the intervention (4415.00 ± 1036.93 pg/g vs. 3679.49 ± 871.18 pg/g, p = 0.0175). The intervention led to an increased abundance of Bifidobacterium breve and Collinsella aerofaciens species, higher alpha-diversity (p < 0.05), and enrichment of functional genes in the gut microbiota related to immunity regulation. Administration of BLa80 at a dose of 5 × 109 CFU/day resulted in a shorter duration of diarrhea and alterations in gut microbiome composition and gene functions.
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Bifidobacterium animalis , Diarrea , Heces , Microbioma Gastrointestinal , Probióticos , Humanos , Método Doble Ciego , Diarrea/terapia , Diarrea/microbiología , Probióticos/uso terapéutico , Probióticos/administración & dosificación , Lactante , Preescolar , Masculino , Femenino , Heces/microbiología , Recién Nacido , Enfermedad Aguda , Resultado del Tratamiento , CatelicidinasRESUMEN
This study explores the pollution characteristics, risks, and sources of heavy metals in small-scale areas. Rongcheng District, Jieyang City, Guangdong Province was considered as the study area and enrichment factor (EF), pollution load index (PLI), potential ecological risk index (RI), and US EPA health risk assessment model were used to evaluate its environmental risk. Moreover, the source apportionment of heavy metals was analyzed through correlation analysis, the characteristic of spatial distribution, and a PMF model. The results showed that the mean concentrations of ω(Cr), ω(Hg), ω(As), ω(Pb), ω(Ni), ω(Cd), ω(Cu), and ω(Zn) were 54.87, 0.25, 8.35, 56.00, 15.38, 0.35, 30.56, and 124.23 mg·kg-1, respectively. The mean concentrations of all elements exceeded the local soil background value. In terms of EF level, Cr, As, Pb, and Ni showed negligible accumulation; Zn and Cu showed minor accumulation; and Hg and Cd showed moderate accumulation. The mean value of the pollution load index was 2.37, with a severe pollution level, and the eight elements were in different pollution levels. In total, the study region suffered severe ecological risk, Hg and Cd presented strong ecological risk, and other elements presented slight ecological risk. The non-carcinogenic risks under the three exposure paths were within the acceptable level. The carcinogenic risks (CR) of adults and children were 9.81E-05 and 5.59E-04, respectively, and Cr and As were the main contributors of CR. The results showed that the four sources of heavy metals were transportation sources (37.02%), parent material sources (18.53%), atmospheric deposition sources (26.49%), and industrial sources (17.96%).
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Mercurio , Metales Pesados , Adulto , Niño , Humanos , Cadmio , Plomo , Carcinógenos , Medición de Riesgo , SueloRESUMEN
Herein, a method based on solubility parameter calculation was first used to analyze microplastics in indoor dust. The limit of quantification (LOQ) reached 0.2 mg/g, and the result of reference material SRM 2585 (n = 3) was 14.8 mg/g ± 1.8 %, suggesting satisfying sensitivity and precision. Recoveries of spiking experiments were > 80 % with no obvious matrix interferences observed, except ethylene propylene diene monomer (EPDM) MPs. Further, 69 indoor dust samples were analyzed to verify the method and to assess exposure scenarios for graduate students in Tianjin, China. EPDM was identified in an indoor environment for the first time as the second most widely detected type after PET in this work. The mass-based result is complementary to the outcomes from thermogravimetric analysis-gas chromatography-mass spectrometry and laser direct infrared imaging. Significant correlations were found between total organic carbon (TOC), microplastics, and BDE-209 concentrations, indicating microplastics important contaminant vectors in indoor dust. Dormitory stays and PET contributed the most to health risks among the three exposure scenarios and detected four polymers, respectively. This work provides an approach with the potential for the standardized determination of microplastics in complex environmental matrices and reveals exposure characteristics of indoor dust microplastics.
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OBJECTIVE: The aim of this study was to assess the efficacy and safety of Bifidobacterium animalis sp. Lactis XLTG11, as an adjunctive treatment for acute watery diarrhea in children, using a randomized, double-blinded, placebo-controlled study design. METHOD: Eligible children with diarrhea were randomly assigned into one of two groups: an intervention group (IG, n = 35), which received conventional treatment plus the probiotic, and a control group (CG, n = 35), which received only conventional treatment. Fecal samples were collected from all children before and after the intervention to measure biochemical indices and analyze gut microbiome (GM) composition. RESULT: The duration of diarrhea (121.3 ± 11.5 h) and hospital length of stay (3.4 ± 1.1 d) in the IG were significantly shorter than those in the CG (133.4 ± 14.1 h and 4 ± 1.3 d, respectively; P < 0.001 and P = 0.041, respectively). A higher percentage of children in the IG showed improvements compared with the CG (57.1% versus 25.7%, P < 0.001). The calprotectin level in the IG was significantly lower than that in the CG after the intervention (928.91 ± 158.90 ng/g versus 1029.86 ± 133.25 ng/g, P = 0.028). XLTG11 administration led to a higher abundance of species B. longum and < breve, increased α-diversity of the GM (P < 0.05), and upregulated the functional genes of the GM related to immunity and nutrient absorption. CONCLUSIONS: Administration of XLTG11 at a dose of 1 × 1010 CFU/d was effective in reducing the duration of diarrhea, inducing beneficial changes in GM composition and gene functions.
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Diarrea , Probióticos , Humanos , Niño , Diarrea/tratamiento farmacológico , Probióticos/uso terapéutico , Bifidobacterium , Heces/microbiología , Método Doble CiegoRESUMEN
In this research, enrichment factor (EF) and pollution load index were utilized to explore the contamination characteristics of toxic elements (TEs) in park dust. The results exhibited that park dust in the study area was mainly moderately polluted, and the EF values of dust Cd, Zn, Pb, Cu and Sb were all > 1. The concentrations of Cr, Cu, Zn and Pb increased with the decrease of dust particle size. The investigation results of chemical speciation and bioavailability of TEs showed that Zn had the highest bioavailability. Three sources of TEs were determined by positive matrix factorization model, Pearson correlation analysis and geostatistical analysis, comprising factor 1 mixed sources of industrial and transportation activities (46.62%), factor 2 natural source (25.56%) and factor 3 mixed source of agricultural activities and the aging of park infrastructures (27.82%). Potential ecological risk (PER) and human health risk (HHR) models based on source apportionment were exploited to estimate PER and HHR of TEs from different sources. The mean PER value of TEs in the park dust was 114, indicating that ecological risk in the study area was relatively high. Factor 1 contributed the most to PER, and the pollution of Cd was the most serious. There were no significant carcinogenic and non-carcinogenic risks for children and adults in the study area. And factor 3 was the biggest source of non-carcinogenic risk, and As, Cr and Pb were the chief contributor to non-carcinogenic risk. The primary source of carcinogenic risk was factor 2, and Cr was the cardinal cancer risk element.
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Monitoreo del Ambiente , Metales Pesados , Adulto , Niño , Humanos , Monitoreo del Ambiente/métodos , Polvo/análisis , Cadmio/toxicidad , Cadmio/análisis , Plomo/análisis , Metales Pesados/toxicidad , Metales Pesados/análisis , Medición de Riesgo/métodos , Carcinógenos/análisis , China , CiudadesRESUMEN
In this study, we compared the concentrations of the heavy metals Cd, Cr, Cu, Zn, Ni, and Pb in the surface soils of urban parks in Wuhan, Hubei Province, with those in the surface soils of urban parks worldwide. The soil contamination data were assessed using enrichment factors and spatial analysis of heavy metals using inverse distance weighting and quantitative analysis of heavy metal sources with a positive definite matrix factor (PMF) receptor model. Further, a probabilistic health risk assessment of children and adults using Monte Carlo simulation was performed. The average Cd, Cr, Cu, Zn, Ni, and Pb concentrations in the surface soils of urban parks were 2.52, 58.74, 31.39, 186.28, 27.00, and 34.89 mg·kg-1, respectively, which exceeded the average soil background values in Hubei. From the inverse distance spatial interpolation map, heavy metal contamination was primarily observed to be present to the southwest of the main urban area. The PMF model resolved four sources: mixed traffic and industrial emission, natural, agricultural, and traffic sources, with relative contributions of 23.9%, 19.3%, 23.4%, and 33.4%, respectively. The Monte Carlo health risk evaluation model demonstrated negligible noncancer risks for both adult and child populations, whereas the health effects of Cd and Cr on children were a concern for cancer risks.
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BACKGROUND: Circadian rhythms are physical and behavioral changes that follow the 24-h cycle of Earth's light and temperature and are regulated by clock genes. Timeless (Tim) has been identified as a canonical clock gene in some insects, however, its functions have been little studied in lepidopteran pests. RESULTS: To investigate Tim (HaTim) gene function in Helicoverpa armigera, an important lepidopteran pest, we obtained the HaTim mutant using the CRISPR/Cas9 gene editing system. Our results showed that the transcript levels of HaTim rhythmically peaked at night in heads of the wild larvae and adult, and the diel expression of HaTim was sensitive to photoperiod and temperature. The expression rhythms of other clock genes, such as HaPer, HaCry1, HaCry2 and HaCwo, were disturbed in the HaTim mutant larvae, as that stage is a sensitivity period for diapause induction. Fifth-instar wild-type larvae could be induced to pupate in diapause under a short-day photoperiod and low temperature, however, fifth-instar HaTim mutant larvae could not be induced under the same conditions. In addition, the emergence of wild-type adults peaked early at night, but the rhythm was disturbed in the HaTim mutant with arrhythmic expression of some clock genes, such as HaPer, HaCry1 and HaCwo in adults. CONCLUSION: Our results suggest that the clock gene Tim is involved in diapause induction and adult emergence in H. armigera, and is a potential target gene for controlling pest. © 2023 Society of Chemical Industry.
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Diapausa , Mariposas Nocturnas , Animales , Ritmo Circadiano/genética , Mariposas Nocturnas/genética , Fotoperiodo , Larva/genética , Larva/metabolismo , MutaciónRESUMEN
Neonicotinoid insecticides (NNIs) had been detected in soil and surface water frequently because of extensive use worldwide, however, data regarding regional characteristics and potential influential factors of sediment were scarce. In the present study, eight NNIs were analyzed in 86 surface sediment samples from different regions (central cities, rural areas and suburbs) and land use types (construction land and crop land) in Jiangsu Province. NNIs were widespread in the sediments, with a mean value of 1.73 ± 0.89 ng g-1 dry weight (dw) (ranged from 0.41 to 3.87 ng g-1 dw). Imidaclothiz (IMIZ), dinotefuran (DIN) and nitenpyram (NIT) were the dominant compounds in the surface sediment, accounted for half of combined total. The results of regional distribution analysis show that NNIs were at higher concentrations in rural areas and crop land, while the residues of NNIs in lakes were more severe compare with rivers in Jiangsu Province. Region characteristics and land use types have an influence on residues of NNIs in surface sediment. Principal component analysis showed that residues of NNIs in surface sediment in Jiangsu Province mainly originated from protect grain crops (maize), fruit (apples, pears) and vegetables in agricultural systems. The residues of NNIs were found to be mostly concentrated in the northwest and northeast in Jiangsu Province, where were the area of intensive agriculture. To investigate the residues of NNIs, while identify the contributing factors, could provide a scientific basis for basic of region environment management and pollution control.
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Insecticidas , Contaminantes Químicos del Agua , Insecticidas/análisis , Lagos/análisis , Neonicotinoides/análisis , Ríos/química , Suelo , Agua/análisis , Contaminantes Químicos del Agua/análisisRESUMEN
The widespread adoption of Bt crops expressing insecticidal proteins derived from Bacillus thuringiensis has created a need to assess the potential effects of these toxins on non-target organisms, especially species such as Arma custos, a generalist predator that provides important biological control services in many field crops in Asia. Direct dietary exposure of A. custos to Cry1Ah and Vip3Aa proteins produced no adverse effects on life history traits, despite continuous exposure throughout development and early adult life to concentrations significantly higher than the Bt protein concentration likely encountered by A.custos in the field, even when feeding directly on Bt plants. Enzyme-linked immunosorbent assay confirmed the presence of Bt proteins in A. custos midguts, but quantitative real-time PCR analysis of 12 genes associated with detoxification, antioxidative responses, immune responses, and metabolism revealed no significant changes in expression in adult bugs. Indirect exposure to these toxins via consumption of intoxicated prey, larvae of Helicoverpa armigera (Hübner), likewise produced no negative impacts on survival, development, adult weight, or female fecundity in either the F0 (exposed) or F1 (unexposed) generation, but female fresh weight was reduced in the F0 generation by the Cry1Ah (50 µg/g) treatment. Finally, a competitive binding assay with labelled protein and a ligand blotting assay both demonstrated that the Cry1Ah protein could not bind to receptors on the midgut brush border membrane vesicles (BBMVs) of A. custos adults. Therefore, we conclude that Cry1Ah and Vip3Aa proteins are unlikely to have significant negative effects on A. custos populations if employed as plant-incorporated protectants in field crops.
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Bacillus thuringiensis , Heterópteros , Animales , Bacillus thuringiensis/genética , Ligandos , Proteínas Bacterianas/genética , Toxinas de Bacillus thuringiensis , Endotoxinas/toxicidad , Endotoxinas/genética , Larva , Proteínas Hemolisinas/toxicidad , Proteínas Hemolisinas/genéticaRESUMEN
Helicoverpa armigera single nucleopolyhedrovirus (HearNPV) has a long coevolutionary history with its host, exerting profound effects on larval development, physiology and immune responses, although the mechanisms mediating these effects remain unclear. We demonstrate that HearNPV infection constrains the growth and development of larvae by inducing high levels of reactive oxygen species (ROS), which increase the expression of forkhead box O transcription factor (FoxO). FoxO upregulates the expression of peroxiredoxin 1 (Prx1) which serves to regulate larval development and immune responses following HearNPV infection. Collectively, our results provide novel insights into the role of Prx1 in larval development and immunity subsequent to HearNPV infection. Further investigation of the oxidative stress induced by HearNPV in H. armigera and its interactions with host immunity could yield novel insights useful in agricultural pest control.
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Baculoviruses can induce climbing behaviour in their caterpillar hosts to ensure they die at elevated positions to enhance virus transmission, providing an excellent model to study parasitic manipulation of host behaviour. Here, we demonstrate that climbing behaviour occurred mostly during daylight hours, and that the height at death of Helicoverpa armigera single nucleopolyhedrovirus (HearNPV)-infected larvae increases with the height of the light source. Phototaxic and electroretinogram (ERG) responses were enhanced after HearNPV-infection in host larvae, and ablation of stemmata in infected larvae prevented both phototaxis and climbing behaviour. Through transcriptome and quantitative PCR, we confirmed that two opsin genes (a blue light-sensitive gene, HaBL; and a long wave-sensitive gene, HaLW) as well as the TRPL (transient receptor potential-like channel protein) gene, all integral to the host's visual perception pathway, were significantly upregulated after HearNPV infection. Knockout of HaBL, HaLW, or TRPL genes using the CRISPR/Cas9 system resulted in significantly reduced ERG responses, phototaxis, and climbing behaviour in HearNPV-infected larvae. These results reveal that HearNPV alters the expression of specific genes to hijack host visual perception at fundamental levels-photoreception and phototransduction-in order to induce climbing behaviour in host larvae.
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Lepidópteros , Nucleopoliedrovirus , Animales , Baculoviridae , Larva/genética , Lepidópteros/fisiología , Nucleopoliedrovirus/genética , Percepción VisualRESUMEN
Non-ferrous metal smelting activities have always been considered as one of the foremost anthropogenic sources of potentially toxic elements (PTEs). The enrichment factor (EF) and pollution load index (PLI) were used to evaluate the pollution level of soil PTEs; positive matrix factorization (PMF), correlation analysis, and geostatistics were utilized to quantify the sources of soil PTEs; and potential ecological risk (PER) and human health risk (HHR) of different sources from farmland, construction land, and natural land were quantifiably determined via combined PTE sources with PER and HHR assessment models. Taking the smelting area of Daye City as an example, the evaluation results of EF and PLI showed that the soil PTE pollution in the study area was serious, especially Cd and Cu. And four sources were quantitatively allocated as agricultural practices (12.14%), traffic emissions (23.07%), natural sources (33.46%), and industrial activities (31.33%). For PER, industrial activities were the largest contributor to PER, accounting for 55.66%, 56.30%, and 55.36% of farmland, construction land, and natural land, respectively, and Cd was the most dangerous element. In terms of HHR, industrial activities were also the cardinal contributors under the three land use types. Children were exposed to serious non-carcinogenic risks under three land use patterns and slight carcinogenic risk in construction land (1.06E - 04). Significantly, the carcinogenic risk of children in farmland (9.06 × 10-5) was very close to the threshold (1 × 10-4), which requires attention. Both non-carcinogenic and carcinogenic risk for adults were all at acceptable levels. The health risks (carcinogenic and non-carcinogenic risks) of children from four different sources were distinctly higher than those of adults. Consequently, strict management and control of industrial activities should be given priority, and the management of agricultural practices should not be ignored.
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Metales Pesados , Contaminantes del Suelo , Adulto , Niño , China , Monitoreo del Ambiente , Contaminación Ambiental/análisis , Humanos , Metales Pesados/análisis , Medición de Riesgo , Suelo , Contaminantes del Suelo/análisisRESUMEN
Large-scale smelting activities release large amounts of potentially toxic elements (PTEs) in fine particles. These particles floating in the air eventually settle on leaves, roads, and even indoors. In smelting areas, indoor environments are generally considered relatively safe. However, these areas are not taken seriously and need to be assessed. This paper systematically studied pollution characteristics, main sources and health risks of ten potentially toxic elements, PTEs (Mn, Ni, Cu, Zn, Hg, Cd, As, Cr, Pb, and Tl), of dust samples from different indoor environments in smelting areas using various methods. Therefore, this study analyzed dust samples from 35 indoor environments. The enrichment factors showed that the indoor dust samples were extremely enriched by Cd and Cu and significantly enriched by Hg, Pb, As, and Zn. The result of the spatial distribution showed that the high-value PTEs were mainly distributed near the Cu smeltery. Three sources were quantitatively assigned for these PTEs, and they were industrial smelting and traffic activities (44.40%), coal-fired activities (18.11%), and natural existence (37.49%). Based on the calculation of health risk, the value of THI for children was 7.57, indicating a significant non-carcinogenic risk. For carcinogenic risk, the values of TCR for children and adults were 2.91×10-2 and 2.97×10-3, respectively, which were much higher than the acceptable risk value 1×10-4. Combining health risk assessment with source discrimination, we found that the industrial discharges and traffic activities were the most main source of non-cancer and cancer risks. Therefore, smelting activities should be more strictly monitored, and traffic emission management should be strengthened.
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Polvo , Metales Pesados , Adulto , Niño , China , Ciudades , Polvo/análisis , Monitoreo del Ambiente , Contaminación Ambiental , Humanos , Metales Pesados/análisis , Medición de RiesgoRESUMEN
To make pollution evaluation of potentially hazardous elements in the soil more accurately, the regional geochemical baseline concentrations of eight potentially hazardous elements (Cr, Ni, Cu, Zn, As, Cd, Hg, and Pb) were established in Huilai County using cumulative frequency distribution curves. Then, the pollution load index and enrichment factor were applied to estimate the contamination levels, based on these geochemical baseline concentrations. The results suggested that topsoil was moderately polluted by potentially hazardous elements, while Cd pollution in the construction land and As pollution in the farmland was relatively severe. The possible sources of eight potentially hazardous elements were analyzed by correlation analysis, geostatistics and positive matrix factorization. Four sources have been determined and apportioned, namely industrial activities, natural sources, agricultural practices, and traffic emissions. Combining the health risk assessment with the source profiles, the health risks quantified from four sources were estimated under farmland, construction land, and woodland. The results showed that agricultural practices were the most main source of non-cancer and cancer risks under woodland and farmland for adults; industrial activities were the most main source of non-cancer and cancer risks under construction land for adults. Children's health risks, both carcinogenic risk and non-carcinogenic risk, were greater than adults, and the health risk trends of adults and children showed similarities. Therefore, agricultural practices under woodland and farmland should be controlled and managed as a priority, while industrial activities should be given priority to control and management under construction land.
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Exposición a Riesgos Ambientales , Metales/análisis , Contaminantes del Suelo/análisis , Suelo/química , China , Monitoreo del Ambiente , Humanos , Medición de RiesgoRESUMEN
Insect metamorphosis is a complex process involving many metabolic pathways, such as juvenile hormones and molting hormones, bioamines, microRNAs (miRNAs), etc. However, relatively little is known about the biogenic amines and their miRNAs to regulate cotton bollworm metamorphosis. Here we show that one miRNA, miR-277 regulates larval-pupal and pupal-adult metamorphosis of cotton bollworm by targeting the 3'UTR of Dopa decarboxylase (DDC), a synthetic catalytic enzyme of dopamine. Injection of miR-277 agomir inhibited the expression of DDC at the mRNA and protein levels, leading to defects in the pupation and emergence of H. armigera that was consistent with the phenotype obtained by injection of DDC double-stranded RNA (dsRNA). Injection of miR-277 antagomir induced the mRNA and protein expression of DDC and rescued the phenotype of pupation failure caused by DDC gene silencing. Unexpectedly, miR-277 antagomir can also cause failure of emergence of H. armigera and both agomir and antagomir of miR-277 injection could cause abnormal phenotypes in wing veins. This study reveals that elaborate regulation of miRNA and its target gene expression is prerequisite for insect development, which provides a new insight to study the developmental mechanisms of insect wing veins.
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Dopa-Decarboxilasa/metabolismo , Metamorfosis Biológica/genética , MicroARNs/metabolismo , Mariposas Nocturnas/genética , Animales , Larva/genética , Larva/crecimiento & desarrollo , Larva/metabolismo , Mariposas Nocturnas/crecimiento & desarrollo , Mariposas Nocturnas/metabolismo , Pupa/genética , Pupa/crecimiento & desarrollo , Pupa/metabolismoRESUMEN
It is necessary to establish local geochemical baseline concentrations (GBCs) due to the lack or the inapplicability of regional background values in the study area. The establishment of GBCs of heavy metal (HM) in soil helps in making the accurate assessment of pollution, and then provides a basis for pollution control. Based on this, a case study was undertaken to study the GBCs of the Jiedong District, Guangdong Province, China. In this research, cumulative frequency distribution curves were utilized to determine the local GBCs in the subsoils. The determined GBCs of Cr, Hg, As, Pb, Ni, Cd, Cu, Zn, Co and V were 39.91, 0.072, 11.48, 47.62, 12.70, 0.17, 14.22, 64.54, 6.31, and 68.14 mg/kg, respectively. The average concentrations of Hg, As, Pb, Cd, Cu and Zn in the topsoils exceeded the corresponding baseline concentrations. In particular, the contents of Cd and Hg were 1.53 and 2.22 times higher than GBCs. According to this baseline criterion, enrichment factor (EF), pollution load index (PLI) and ecological risk index (RI) were applied to assessing HM pollution. EF and PLI suggested that most areas were under moderate contamination, while Hg and Cd pollution was more serious. And the RI values presented that the potential ecological risks were low in most parts of the study area. The possible origins of HMs were identified by combining positive matrix factorization with EF and geostatistics. Comprehensive analysis indicated that Hg and Cd were related to industrial activities, such as textile and garment processing, plastic and rubber production and metal manufacturing. Arsenic and part of Cu mainly came from agricultural activities, namely the use of pesticides, fertilizers and livestock manures. Lead and Zn were mainly attributed to traffic emissions. Chromium, Ni, V, Co, and part of Cu were originated from natural source controlled by parent materials. The corresponding contributions of these sources were 20.61%, 24.20%, 19.22% and 35.97%, respectively. This work provides information to prevent and control the soil HM pollution by proposing the efficient management of anthropogenic sources.
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Municipal wastewater treatment plants (MWTPs) serve an essential role in reducing mercury (Hg) pollution. However, few studies quantified the transport and transformation of Hg through MWTPs, particularly plants based on the anaerobic-anoxic-aerobic (A2/O) process. Here, we present a mass balance for total mercury (THg) and total methylmercury (TMeHg) at the plant, and investigate the influence of pH, temperature, and dissolved oxygen on the occurrence and fate of methylmercury (MeHg) in the system. The concentrations of the THg and TMeHg in the raw sewage were 40.3 ± 26.6 ng/L (4.3 ± 2.7 g/day) and 1.9 ± 0.6 ng/L (193 ± 58 mg/day), respectively. Their concentrations in the plant's effluent water were 7.4 ± 1.5 ng/L (0.74 ± 0.2 g/day) and 0.04 ± 0.01 ng/L (3.9 ± 1.0 mg/day), corresponding to decreases of ~82% for THg and ~98% for TMeHg. Within the plant, only ~10% of the THg was removed with primary sedimentation, as Hg in the raw sewage was predominately in dissolved form. In contrast, a significant portion of TMeHg (~43%) was associated with incoming particulate matter. Much of the remaining Hg was removed in subsequent A2/O process and secondary clarifiers, with a ~78% of the THg entering the plant transferring to the dewatered sludge (concentration 1.05 ± 0.28 µg/g; 3.2 ± 0.8 g/day). These same steps decreased TMeHg in the water by ~95%, with <10% of that reduction being TMeHg transferred to the sludge (concentration 2.1 ± 1.1 ng/g; 6.2 ± 3.3 mg/day), suggesting >90% TMeHg degradation. In addition, the most important factor that impacted the variation of TMeHg concentrations was pH, then was temperature. Dissolved oxygen showed no relationship with TMeHg and DMeHg. Overall, this study demonstrates that A2/O MWTPs effectively remove MeHg from wastewater, however, sludge remains an important potential source of Hg to the environment.
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Heavy metals (HMs) in soil cause adverse effects on ecosystem and human health. Quantifying ecological risk and human health risk (HHR) from sources can determine priority sources and help to mitigate the risks. In this research, geostatistics and positive matrix factorization (PMF) were used to identify and quantify the sources of soil HMs; and then ecological risk and HHR from different sources under woodland, construction land and farmland were quantitatively calculated by combining the potential ecological risk index (RI) and HHR assessment models with PMF model. Taking Jiedong District as an example, four sources were quantitatively apportioned, which were agricultural practices (23.08%), industrial activities (29.10%), natural source (22.87%) and traffic emissions (24.95%). For ecological risk, industrial activities were the greatest contributor, accounting for about 49.71%, 48.11% and 47.15% under construction land, woodland and farmland, respectively. For non-carcinogenic risk, agricultural practices were the largest source under woodland and farmland, while industrial activities were the largest source under construction land. As for carcinogenic risk, no matter which kind of land use, agricultural practices were the largest source. In addition, the health risks of children, including non-carcinogenic and carcinogenic risks, were higher than those of adults, and the trends in health risks for children and adults were similar. The integrated approach was useful to evaluate ecological risk and HHR quantification from sources under different land use, thereby providing valuable suggestions for reducing pollution and protecting human health from the sources.