Characterization of Salmonella Phage P1-CTX and the Potential Mechanism Underlying the Acquisition of the blaCTX-M-27 Gene.

The P1 phage has garnered attention as a carrier of antibiotic resistance genes (ARGs) in Enterobacteriaceae. However, the transferability of ARGs by P1-like phages carrying ARGs, in addition to the mechanism underlying ARG acquisition, remain largely unknown. In this study, we elucidated the biological characteristics, the induction and transmission abilities, and the acquisition mechanism of the blaCTX-M-27 gene in the P1 phage. The P1-CTX phage exhibited distinct lytic plaques and possessed a complete head and tail structure. Additionally, the P1-CTX phage was induced successfully under various conditions, including UV exposure, heat treatment at 42 °C, and subinhibitory concentrations (sub-MICs) of antibiotics. Moreover, the P1-CTX phage could mobilize the blaCTX-M-27 gene into three strains of Escherichia coli (E. coli) and the following seven different serotypes of Salmonella: Rissen, Derby, Kentucky, Typhimurium, Cerro, Senftenberg, and Muenster. The mechanism underlying ARG acquisition by the P1-CTX phage involved Tn1721 transposition-mediated movement of blaCTX-M-27 into the ref and mat genes within its genome. To our knowledge, this is the first report documenting the dynamic processes of ARG acquisition by a phage. Furthermore, this study enriches the research on the mechanism underlying the phage acquisition of drug resistance genes and provides a basis for determining the risk of drug resistance during phage transmission.

Antagonistic activity and mechanism of Bacillus subtilis CG-6 suppression of root rot and growth promotion in Alfalfa.

Root rot is a common disease, that severely affects the yield and quality of alfalfa. Biocontrol is widely used to control plant diseases caused by pathogenic fungi, however, biocontrol strains for alfalfa root rot are very limited. In this study, a Bacillus subtilis CG-6 strain with a significant biocontrol effect on alfalfa root rot was isolated. CG-6 secretes antibacterial enzymes and siderophore, phosphate solubilization and indoleacetic acid (IAA). The inhibition rate of strain CG-6 against Fusarium oxysporum was 87.33%, and it showed broad-spectrum antifungal activity. Inoculation with CG-6 significantly reduced the incidence of alfalfa root rot, the control effect of greenhouse cultivation reached 58.12%, and CG-6 treatment significantly increased alfalfa plant height, root length, fresh weight, and dry weight. The treatment with CG-6 significantly increased the levels of antioxidant enzymes (catalase, peroxidase, superoxide dismutase, and lipoxygenase) in alfalfa leaves by 15.52%-34.03%. Defensive enzymes (chitinase and ß-1,3-glucanase) increased by 24.37% and 28.08%, respectively. The expression levels of regulatory enzyme genes (MsCAT, MsPOD, MsCu, Zn-SOD1, MsCu, Zn-SOD2, MsCu, Zn-SOD3, and MsLOX2) and systemic resistance genes (MsPR1, MsPDF1.2, and MsVSP2) increased by 0.50-2.85 fold, which were higher than those in the pathogen treatment group. Therefore, CG-6 could be used as a potential strain to develop biopesticides against alfalfa root rot.

Metabolomic and physiological analysis of alfalfa (Medicago sativa L.) in response to saline and alkaline stress.

Alfalfa (Medicago sativa L.) is a leguminous forage widely grown worldwide. Saline and alkaline stress can affect its development and yield. To elucidate the physiological mechanisms of alfalfa in response to saline and alkaline stress, we investigated the growth and physiological and metabolomic changes in alfalfa under saline (100 mM NaCl) and alkaline (100 mM Na2CO3, NaHCO3) stress. At the same Na+ concentration, alkaline stress caused more damage than that caused by saline stress. A total of 65 and 124 metabolites were identified in response to saline and alkaline stress, respectively. Determination of gene expression, enzyme activity, substance content, and KEGG enrichment analysis in key pathways revealed that alfalfa responded to saline stress primarily by osmoregulation and TCA cycle enhancement. Flavonoid synthesis, TCA cycle, glutamate anabolism, jasmonate synthesis, and cell wall component synthesis increased as responses to alkaline stress. This study provides important resources for breeding saline-alkaline-resistant alfalfa.

CsPM5.2, a phosphate transporter protein-like gene, promotes powdery mildew resistance in cucumber.

Powdery mildew (PM) is one of the most serious fungal diseases affecting cucumbers (Cucumis sativus L.). The mechanism of PM resistance in cucumber is intricate and remains fragmentary as it is controlled by several genes. In this study, we detected the major-effect Quantitative Trait Locus (QTL), PM5.2, involved in PM resistance by QTL mapping. Through fine mapping, the dominant PM resistance gene, CsPM5.2, was cloned and its function was confirmed by transgenic complementation and natural variation identification. In cultivar 9930, a dysfunctional CsPM5.2 mutant resulted from a single nucleotide polymorphism in the coding region and endowed susceptibility to PM. CsPM5.2 encodes a phosphate transporter-like protein PHO1; H3. The expression of CsPM5.2 is ubiquitous and induced by the PM pathogen. In cucumber, both CsPM5.2 and Cspm5.1 (Csmlo1) are required for PM resistance. Transcriptome analysis suggested that the salicylic acid (SA) pathway may play an important role in CsPM5.2-mediated PM resistance. Our findings help parse the mechanisms of PM resistance and provide strategies for breeding PM-resistant cucumber cultivars.

Nickel molybdate/cobalt iron carbonate hydroxide heterojunction with oxygen vacancy enables interfacial synergism to trigger oxygen evolution reaction.

The development of electrocatalysts with excellent performance toward oxygen evolution reaction (OER) for the production of hydrogen is of great significance to alleviate energy crisis and environmental pollution. Herein, the heterostructure (NMO/FCHC-0.4) was fabricated by the coupling growth of NiMoO4 (NMO) and cobalt iron carbonate hydroxide (FCHC) on nickel foam as an electrocatalyst for OER. The interfacial synergy on NMO/FCHC-0.4 heterojunction can promote the interfacial electron redistribution, affect the center position of d band, optimize the adsorption of intermediate, and improve the conductivity. Beyond, oxygen defect sites are conducive to the adsorption of intermediates, and increase the number of active sites. Real-time OER kinetic simulation revealed that the interfacial synergism and molybdate could reduce the adsorption of hydroxide, promote the deprotonation step of M-OH, and facilitate the formation of M-OOH (M represents the metal active site). As a result, NMO/FCHC-0.4 displays excellent OER electrocatalytic performance with an overpotential of 250/280 mV at the current density 100/200 mA cm-2 and robust stability at 100 mA cm-2 for 100 h. This work provides deep insights into the roles of interfacial electronic modulation and oxygen vacancy to design high-efficiency electrocatalysts for OER.

Electronic modulation and reaction-pathway optimization on three-dimensional seaweed-like NiSe@NiMn LDH heterostructure to trigger effective oxygen evolution reaction.

The development of low-cost and high-efficiency electrocatalysts for the oxygen evolution reaction (OER) is essential to produce high-purity hydrogen in large scale. Herein, a three-dimensional (3D) seaweed-like hierarchical structure was fabricated using two-dimensional (2D) NiMn LDH nanosheets wrapped on one-dimensional (1D) NiSe nanowires with nickel foam (NF) as a substrate (NiSe@NiMn LDH/NF) via hydrothermal and electrodeposition processes. Owing to the strong interfacial synergy, 3D seaweed-like hierarchical structure, higher conductivity, and strong structural stability, the NiSe@NiMn LDH/NF exhibited superior OER performance with an overpotential of 287 mV at 100 mA cm-2, and stably operated for 160 h at large current. Moreover, the overall water splitting system with NiSe@NiMn LDH/NF as the anode and Pt/C/NF as the cathode exhibited a low cell voltage of 1.59/1.64 V to reach 50/100 mA cm-2, and excellent stability for 110 h at 300 mA cm-2. The density function theory (DFT) calculations unveiled that NiSe@NiMn LDH enabled the interfacial synergy, reallocating the electron density at the interface, and further weakening the energy barrier of OH* by strengthening chemical bonds with OH* intermediates to improve the intrinsic OER activity.

Coupling effect and electronic modulation for synergistically enhanced overall alkaline water splitting on bifunctional Fe-doped CoBi/CoP nanoneedle arrays.

Designing bifunctional electrocatalysts with high efficiency and low cost for water splitting is urgently required for the production of green hydrogen. Herein, a bifunctional iron-doped cobalt borate/cobalt phosphide hybrid supported on nickel foam (Fe-CoBi/CoP/NF) was fabricated via hydrothermal and phosphating process. Benefit from the unique nanoneedle architecture for faster mass transfer, the existence of borate on CoBi for accelerating proton transfer, the moderate adsorption of H* species on CoP, Fe doping and the synergistic effect between CoBi and CoP, Fe-CoBi/CoP/NF hybrid exhibits a low overpotential of 137 mV and 260 mV at 100 mA cm-2 for hydrogen evolution reaction (HER) and oxygen evolution reaction (OER), respectively. Moreover, Fe-CoBi/CoP/NF||Fe-CoBi/CoP/NF also presents a low cell potential of 1.65 V@100 mA cm-2 for overall alkaline water splitting and excellent durability (128 h) without decay. This work provides a new insight into the design of bifunctional electrocatalysts simultaneously through the morphological engineering and heteroatomic doping.

Isolation and Identification of a Phosphate-Solubilizing Pantoea dispersa with a Saline-Alkali Tolerance and Analysis of Its Growth-Promoting Effects on Silage Maize Under Saline-Alkali Field Conditions.

Phosphate-solubilizing bacteria (PSB) are microorganisms that can dissolve insoluble phosphorus (P) to accessible forms. This study aimed to screen saline-alkali-tolerant PSB and analyze its growth promoting properties, and evaluate its effects on the growth, quality, soil nutrient balance, and enzyme activities of silage maize in the field. We isolated six phosphate-solubilizing strains from rhizosphere soil of silage maize planted in saline-alkali land, and FC-1 with the best P-solubilizing effect was used for further study. The morphological, physiological and biochemical analysis, and 16S rDNA and housekeeping gene atpD sequencing were performed for identification. FC-1 was identified as Pantoea dispersa and had high P solubility. The phosphate solubility of FC-1 using four P sources ranged from 160.79 to 270.22 mg l-1. FC-1 produced indole-3-acetic acid (IAA) and decreased the pH of the growth media by secreting organic acids, including citric acid, malic acid, succinic acid, and acetic acid. The results of a field experiment indicated that FC-1 treatment increased the height, stem diameter, fresh weight, dry weight, starch content, crude protein content, and total P content of silage maize by 9.8, 9.2, 12.6, 11.7, 12.6, 18.3, and 17.4%, respectively. The nitrogen, potassium, phosphorus, and organic matter contents in the rhizosphere soil of silage maize increased by 29.8, 17.1, 17.9, and 25.3%, respectively; urease, catalase, sucrase, and alkaline phosphatase levels also increased by 24.7, 26.7, 24.0, and 19.5%, respectively. FC-1 promoted the growth of silage maize by improving nutrient metabolism and enzyme activities in saline-alkali soil and may be an effective alternative to fertilizers.

La and S Co-Doping Induced the Synergism of Multiphase Nickel-Iron Nanosheets with Rich Oxygen Vacancies to Trigger Large-Current-Density Oxygen Evolution and Urea Oxidation Reactions.

The development of cost-effective electrocatalysts for oxygen evolution reaction (OER) and urea oxidation reaction (UOR) is of great significance for hydrogen production. Herein, La and S co-doped multiphase electrocatalyst (LSFN-63) is fabricated by metal-corrosion process. FeOOH can reduce the formation energy of NiOOH, and enhance the stability of NiOOH as active sites for OER/UOR. The rich oxygen vacancies can increase the number of active sites, optimize the adsorption of intermediates, and improve electrical conductivity. Beyond, La and S co-doping can also regulate the electronic structure of FeOOH. As a result, LSFN-63 presents a low overpotential of 210/450 mV at 100/1000 mA cm-2 , small Tafel slope (32 mV dec-1 ), and outstanding stability under 1000 mA cm-2 @60 h, and can also display excellent OER activity with 180 mV at 250 mA cm-2 and long-term catalytic durability at 250 mA cm-2 @135 h in 30 wt% KOH under 60 °C. Moreover, LSFN-63 demonstrates remarkable UOR performance in 1 m KOH + 0.5 m urea, which just requires an ultra-small overpotential of 140 mV at 100 mA cm-2 , and maintain long-term durability over 120 h. This work opens up a promising avenue for the development of high-efficiency electrocatalysts by a facile metal-corrosion strategy.

The engagement of histone lysine methyltransferases with nucleosomes: structural basis, regulatory mechanisms, and therapeutic implications.

Histone lysine methyltransferases (HKMTs) deposit methyl groups onto lysine residues on histones and play important roles in regulating chromatin structure and gene expression. The structures and functions of HKMTs have been extensively investigated in recent decades, significantly advancing our understanding of the dynamic regulation of histone methylation. Here, we review the recent progress in structural studies of representative HKMTs in complex with nucleosomes (H3K4, H3K27, H3K36, H3K79, and H4K20 methyltransferases), with emphasis on the molecular mechanisms of nucleosome recognition and trans-histone crosstalk by these HKMTs. These structural studies inform HKMTs' roles in tumorigenesis and provide the foundations for developing new therapeutic approaches targeting HKMTs in cancers.

Mapping and identification of CsSF4, a gene encoding a UDP-N-acetyl glucosamine-peptide N-acetylglucosaminyltransferase required for fruit elongation in cucumber (Cucumis sativus L.).

KEY MESSAGE: The short fruit length phenotype in sf4 is caused by a SNP in Csa1G665390, which encodes an O-linked N-acetylglucosamine (GlcNAc) transferase in cucumber. Cucumber fruit is an excellent resource for studying fruit morphology due to its fast growth rate and naturally abundant morphological variations. The regulatory mechanisms underlying plant organ size and shape are important and fundamental biological questions. In this study, a short-fruit length mutant, sf4, was identified from an ethyl methanesulfonate (EMS) mutagenesis population derived from the North China-type cucumber inbred line WD1. Genetic analysis indicated that the short fruit length phenotype of sf4 was controlled by a recessive nuclear gene. The SF4 locus was located in a 116.7-kb genomic region between the SNP markers GCSNP75 and GCSNP82 on chromosome 1. Genomic and cDNA sequences analysis indicated that a single G to A transition at the last nucleotide of Csa1G665390 intron 21 in sf4 changed the splice site from GT-AG to GT-AA, resulting in a 42-bp deletion in exon 22. Csa1G665390 is presumed to be a candidate gene, CsSF4 that encodes an O-linked N-acetylglucosamine (GlcNAc) transferase (OGT). CsSF4 was highly expressed in the leaves and male flowers of wild-type cucumbers. Transcriptome analysis indicated that sf4 had alterations in expression of many genes involved in hormone response pathways, cell cycle regulation, DNA replication, and cell division, suggesting that cell proliferation-associated gene networks regulate fruit development in cucumber. Identification of CsSF4 will contribute to elucidating the function of OGT in cell proliferation and to understanding fruit elongation mechanisms in cucumber.

Tectonic Implication of the 2022 MS 6.9 Earthquake in Menyuan, Qinghai, China: Analysis of Precise Earthquake Locations and InSAR.

Precise earthquake locations and InSAR (Interferometric Synthetic Aperture Radar) deformation observation are the major methods to understand the earthquake occurrence and disaster-causing process. This paper proposes a processing framework for analyzing strong earthquake mechanisms from one-dimensional velocity inversion to precise earthquake locations combined with InSAR deformation observation, and discusses earthquake-generating fault and dynamic mechanisms of tectonic deformation. We analyzed the Menyuan Ms 6.9 earthquake in 2022 and discuss the historical seismic activities and corresponding stress adjustment processes in the research region. To analyze and study the seismogenic structure and mechanism of the earthquake, we investigated the spatial and temporal distribution characteristics of the Menyuan earthquake sequence and analyzed the InSAR coseismic deformation field. We obtained the precise locations of the main shock and aftershocks and the coseismic InSAR deformation field of the main shock. It was confirmed that the Ms 6.9 earthquake was a shallow sinistral strike-slip earthquake, which led to the sequential activation of the Tuolaishan and Lenglongling faults. The main seismogenic fault of the mainshock was the northwestern end of the Lenglongling fault, and the earthquake rupture was segmented. It can be inferred that the earthquake was a stress-adjusted event triggered in the Qilian-Haiyuan tectonic belt caused by the northeasterly push of the Qinghai-Tibet Plateau. The risk of moderate to high earthquakes in the region remains high in the future, requiring enhanced seismic observations.

RPS14 promotes the development and progression of glioma via p53 signaling pathway.

Glioma is a common primary intracranial brain disease that exhibits an increasing incidence and mortality rate. Accumulating evidences have suggested that Ribosomal protein S14 (RPS14) was involved in cell proliferation and tumor progression. Nevertheless, the biological function and underlying mechanism of RPS14 in glioma are still largely unclear. Herein, we found that RPS14 was overexpressed in glioma. In the loss-of-function experiments, RPS14 depletion markedly suppressed glioma cell proliferation, migration and prompted cell apoptosis in vitro. Further study suggested that RPS14 depletion inhibited tumor growth of glioma in vivo. Additionally, human phospho-kinase array profiling and Western blot analysis revealed that the effects of RPS14 knockdown on glioma may be closely associated with p53 signaling pathway. Further study indicated that addition of p53 inhibitor pifithrin-α (PFT-α) could attenuate the influences of RPS14 knockdown on cell proliferation and apoptosis. Taken together, our findings suggested that RPS14 exhibits a pro-oncogenic role in glioma progression and may be act as a novel potential therapeutic target for gliomas.

Self-sacrificial reconstruction of MoO42- intercalated NiFe LDH/Co2P heterostructures enabling interfacial synergies and oxygen vacancies for triggering oxygen evolution reaction.

Exploring high-efficiency electrocatalysts for oxygen evolution reaction (OER) is one of the most important concerns to produce hydrogen in water electrolysis. Herein, the FNM/Co2P-0.4 heterostructure was designed as an electrocatalyst for the OER process by the combination of MoO42- intercalating NiFe LDH and Co2P on nickel foam (NF). The surface reconstruction and MoO42- leaching can induce the conversion of Co2P and NiFe LDH on FNM/Co2P-0.4 to generate Co/NiOOH with more oxygen vacancies. Beyond, CoOOH and NiOOH can also synergize to reduce the energy barrier of OER, optimize conductivity, and improve stability. The surface reconstruction and the formation of OOH⁎ were further unveiled by in-situ UV-vis absorption spectra and Fourier-transformed alternative current voltammetry (FTACV). The integration of interfacial synergies and oxygen vacancies can facilitate the adsorption/desorption of intermediates, regulate the d-band center, and expose more active sites. And as a result, FNM/Co2P-0.4 shows a significant low overpotential (240 mV) at 50 mA cm-2, a small Tafel (74 mV dec-1), low activation energy (Ea) and remarkable durability. This work provides a new pathway to improve the OER performance by using interfacial synergies and rich oxygen vacancies derived from the self-sacrificial reconstruction of heterostructured electrocatalysts.

Doping Mo into NiFe LDH/NiSe Heterostructure to Enhance Oxygen Evolution Activity by Synergistically Facilitating Electronic Modulation and Surface Reconstruction.

It is of great significance to design highly efficient electrocatalysts with abundant earth elements instead of precious metals for water splitting. Herein, Mo-doped NiFe-layered double hydroxides/NiSe heterostructure (Mo-NiFe LDH/NiSe) was fabricated by coupling Mo-doped NiFe LDH and NiSe on nickel foam (NF). The heterostructure electrocatalyst showed ultra-low overpotential (250 mV) and remarkable durability for oxygen evolution reaction (OER) at 150 mA cm-2 . Both theoretical and experimental results confirmed that Mo doping and interfacial synergism induced the interfacial charge redistribution and the lifted d-band center to weaken the energy barrier (EB) of the formation of OOH* . Mo doping also facilitated the surface reconstruction of NiFe LDH into Ni(Fe)OOH as the active sites under electro-oxidation process. This work provides a facile strategy for electronic modulation and surface reconstruction of OER electrocatalyst by transition metal doping and heterostructure generation.

Synergistic coupling of heterostructured porous CoP nanosheets with P doped NiO for highly efficient overall alkaline water splitting.

Exploring non-noble metal materials as bifunctional catalysts for water electrolysis is of great significance for the development and utilization of hydrogen energy. Herein, a flower branch-leaf shaped phosphide/oxide heterogeneous electrocatalyst located on Ni foam (CoP/P-NiO/NF) was developed through hydrothermal and phosphorization strategy. Benefiting from the strong ability to dissociate H2O molecules on P-NiO and the suitable adsorption of intermediate H species on CoP, the optimal CoP/P-NiO/NF exhibited outstanding performance with low overpotentials of 52 mV at current density of 10 mA cm-2, smaller Tafel slopes of 73.6 mV dec-1 for hydrogen evolution reaction (HER). Meanwhile, CoP/P-NiO/NF indicated 265 mV at 100 mA cm-2 with Tafel slope of 101.8 mV dec-1 for oxygen evolution reaction (OER) due to the optimal redistribution of electrons among Ni2+, Co2+ and Co3+ for favorable adsorption/desorption of oxygen-intermediates. Both HER and OER shown robust stability during 32 h without decline. The corresponding two-electrode system for overall alkaline water splitting required a low voltage of 1.6 V at 100 mA cm-2 with long stability (20 h) which is far lower than that on RuO2-Pt/C and many other reported non-noble metal electrocatalysts. This work demonstrates that the synergistic effect and morphology engineering play vital roles in the enhanced electrocatalytic performance.

Comparative Transcriptome Analysis of Hard and Tender Fruit Spines of Cucumber to Identify Genes Involved in the Morphological Development of Fruit Spines.

The spines of cucumber fruit not only have important commercial value but are also a classical tissue to study cell division and differentiation modes of multicellular trichomes. It has been reported that CsTs (C-type Lectin receptor-like kinase) can influence the development of fruit spines. In this study, we took a pair of cucumber materials defined as hard (Ts, wild type) and tender spines (ts, mutant) and defined the developmental process of fruit spines as consisting of four stages (stage I to stage IV) by continuously observing by microscope and SEM. Comparisons of transcriptome profiles at different development stages of wild-type spines showed that 803 and 722 genes were upregulated in the stalk (stage II and stage III) and base (stage IV) development stages of fruit spines, respectively. The function analysis of DEGs showed that genes related to auxin polar transport and HD-ZIP transcription factor are significantly upregulated during the development of the stalk. bHLH transcription factors and cytoskeleton-related genes were significantly upregulated during the development of the base. In addition, stage III is the key point for the difference between wild-type and mutant spines. We detected 628 DEGs between wild type and mutant at stage III. These DEGs are mainly involved in the calcium signaling of the cytoskeleton and auxin polar transport. Coincidentally, we found that CsVTI11, a factor involved in auxin signal transmission, can interact with CsTs in vivo, but this interaction does not occur between CsVTI11 and Csts, further suggesting that CsTs may regulate the development of fruit spines by influencing cell polarity. These results provide useful tools to study the molecular networks associated with cucumber fruit spine development and elucidate the biological pathways that C-type Lectin receptor-like kinase plays in regulating the development of fruit spines.

IS26 Is Responsible for the Evolution and Transmission of blaNDM-Harboring Plasmids in Escherichia coli of Poultry Origin in China.

Carbapenem-resistant Enterobacteriaceae are some of the most important pathogens responsible for nosocomial infections, which can be challenging to treat. The blaNDM carbapenemase genes, which are expressed by New Delhi metallo-ß-lactamase (NDM)-producing Escherichia coli isolates, have been found in humans, environmental samples, and multiple other sources worldwide. Importantly, these genes have also been found in farm animals, which are considered an NDM reservoir and an important source of human infections. However, the dynamic evolution of blaNDM genetic contexts and blaNDM-harboring plasmids has not been directly observed, making it difficult to assess the extent of horizontal dissemination of the blaNDM gene. In this study, we detected NDM-1 (n = 1), NDM-5 (n = 24), and NDM-9 (n = 8) variants expressed by E. coli strains isolated from poultry in China from 2016 to 2017. By analyzing the immediate genetic environment of the blaNDM genes, we found that IS26 was associated with multiple types of blaNDM multidrug resistance regions, and we identified various IS26-derived circular intermediates. Importantly, in E. coli strain GD33, we propose that IncHI2 and IncI1 plasmids can fuse when IS26 is present. Our analysis of the IS26 elements flanking blaNDM allowed us to propose an important role for IS26 elements in the evolution of multidrug-resistant regions (MRRs) and in the dissemination of blaNDM. To the best of our knowledge, this is the first description of the dynamic evolution of blaNDM genetic contexts and blaNDM-harboring plasmids. These findings could help proactively limit the transmission of these NDM-producing isolates from food animals to humans. IMPORTANCE Carbapenem resistance in members of the order Enterobacterales is a growing public health problem that is associated with high mortality in developing and industrialized countries. Moreover, in the field of veterinary medicine, the occurrence of New Delhi metallo-ß-lactamase-producing Escherichia coli isolates in animals, especially food-producing animals, has become a growing concern in recent years. The wide dissemination of blaNDM is closely related to mobile genetic elements (MGEs) and plasmids. Although previous analyses have explored the association of many different MGEs with mobilization of blaNDM, little is known about the evolution of various genetic contexts of blaNDM in E. coli. Here, we report the important role of IS26 in forming multiple types of blaNDM multidrug resistance cassettes and the dynamic recombination of plasmids bearing blaNDM. These results suggest that significant attention should be paid to monitoring the transmission and further evolution of blaNDM-harboring plasmids among E. coli strains of food animal origin.

TERMINAL FLOWER 1 and TERMINAL FLOWER 1d respond to temperature and photoperiod signals to inhibit determinate growth in cucumber.

Plants monitor environmental cues to balance their vegetative and productive growth by optimizing their inflorescence architecture. TERMINAL FLOWER 1 (TFL1) and its orthologs regulate the inflorescence structure in cucumber, yet the mechanisms underlying their responses to environmental factors and the formation of terminal flowers remain elusive. Here, we performed map-based cloning to identify the gene that controls a season-dependent determinate growth phenotype and found that it was caused by the complete deletion of CsTFL1 in the genome of cucumber line WI1983Hde. In the CsTFL1 deletion plants (CsTFL1del ), determinate growth could be partially rescued by high-temperature and long-day conditions. The expressions of CsTFL1 and its ortholog CsTFL1d could be upregulated by long-day and high-temperature signals. Knockdown of CsTFL1d resulted in determinate growth and the formation of terminal flowers in WT. These results indicate that the induction of CsTFL1d expression by long-day and high-temperature might partially rescue determinate growth of CsTFL1del . Furthermore, biochemical analyses showed that CsTFL1d interacts directly with CsNOT2a, which indicated that CsTFL1d and CsTFL1 function via similar regulatory mechanism. Our data suggest that CsTFL1 and CsTFL1d co-contribute to inhibit determinate growth by responding to temperature and photoperiod signals. It provides mechanistic insights into how environmental cues sculpt the inflorescence architecture of cucumber.

A SNP of HD-ZIP I transcription factor leads to distortion of trichome morphology in cucumber (Cucumis sativus L.).

BACKGROUND: Trichomes are excellent model systems for the analysis of cell differentiation and play essential roles in plant protection. From cucumber inbred line 'WD1', we identified an EMS-induced trichome abnormally developing mutant, nps, which exhibited smaller, denser and no pyramid-shaped head trichomes. RESULTS: Using F2 and BC1 populations constructed from a cross between nps and '9930', the genetic analysis showed that the nps trait is controlled by a single recessive nuclear gene. We identified CsNps by map-based cloning with 576 individuals of the F2 population generated from the cross of nps and inbred line '9930'. The CsNps was located at a 13.4-kb genomic region on chromosome 3, which region contains three predicted genes. Sequence analysis showed that only one single nucleotide mutation (C → T) between 9930 and nps was found in the second exon of Csa3G748220, a plant-specific class I HD-Zip gene. The result of allelism test also indicated that nps is a novel allelic mutant of Mict (Micro-trichome). Thus, nps was renamed mict-L130F. By comparing the transcriptome of mict-L130F vs WD1 and 06-2 (mict) vs 06-1 (wildtype, near-isogenic line of 06-2), several potential target genes that may be related to trichome development were identified. CONCLUSIONS: Our results demonstrate that Mict-L130F is involved in the morphogenesis of trichomes. Map-based cloning of the Mict-L130F gene could promote the study of trichome development in cucumber.