Redesigning a S-nitrosylated pyruvate-dependent GABA transaminase 1 to generate high-malate and saline-alkali-tolerant tomato.

Although saline-alkali stress can improve tomato quality, the detailed molecular processes that balance stress tolerance and quality are not well-understood. Our research links nitric oxide (NO) and γ-aminobutyric acid (GABA) with the control of root malate exudation and fruit malate storage, mediated by aluminium-activated malate transporter 9/14 (SlALMT9/14). By modifying a specific S-nitrosylated site on pyruvate-dependent GABA transaminase 1 (SlGABA-TP1), we have found a way to enhance both plant's saline-alkali tolerance and fruit quality. Under saline-alkali stress, NO levels vary in tomato roots and fruits. High NO in roots leads to S-nitrosylation of SlGABA-TP1/2/3 at Cys316/258/316, reducing their activity and increasing GABA. This GABA then reduces malate exudation from roots and affects saline-alkali tolerance by interacting with SlALMT14. In fruits, a moderate NO level boosts SlGABA-TP1 expression and GABA breakdown, easing GABA's block on SlALMT9 and increasing malate storage. Mutants of SlGABA-TP1C316S that do not undergo S-nitrosylation maintain high activity, supporting malate movement in both roots and fruits under stress. This study suggests targeting SlGABA-TP1Cys316 in tomato breeding could significantly improve plant's saline-alkali tolerance and fruit quality, offering a promising strategy for agricultural development.

A genome assembly of ginger (Zingiber officinale Roscoe) provides insights into genome evolution and 6-gingerol biosynthesis.

Ginger is cultivated in tropical and subtropical regions and is one of the most crucial spices worldwide owing to its special taste and scent. Here, we present a high-quality genome assembly for 'Small Laiwu Ginger', a famous cultivated ginger in northern China. The ginger genome was phased into two haplotypes, haplotype A (1.55Gb), and haplotype B (1.44Gb). Analysis of Ty1/Copia and Ty3/Gypsy LTR retrotransposon families revealed that both have undergone multiple retrotransposon bursts about 0-1 million years ago. In addition to a recent whole-genome duplication event, there has been a lineage-specific expansion of genes involved in stilbenoid, diarylheptanoid, and gingerol biosynthesis, thereby enhancing 6-gingerol biosynthesis. Furthermore, we focused on the biosynthesis of 6-gingerol, the most important gingerol, and screened key transcription factors ZoMYB106 and ZobHLH148 that regulate 6-gingerol synthesis by transcriptomic and metabolomic analysis in the ginger rhizome at four growth stages. The results of yeast one-hybrid, electrophoretic mobility shift, and dual-luciferase reporter gene assays showed that both ZoMYB106 and ZobHLH148 bind to the promoters of the key rate-limiting enzyme genes ZoCCOMT1 and ZoCCOMT2 in the 6-gingerol synthesis pathway and promote their transcriptional activities. The reference genome, transcriptome, and metabolome data pave the way for further research on the molecular mechanism underlying the biosynthesis of 6-gingerol. Furthermore, it provides precious new resources for the study on the biology and molecular breeding of ginger.

Uncovering the dominant role of root lignin accumulation in silicon-induced resistance to drought in tomato.

The role of lignin accumulation in silicon-induced resistance has not been fully elucidated. Based on the finding that the root cell wall is protected by silicon, this study explored the role of lignin accumulation in silicon-induced drought resistance in tomato. The decreased silicon concentration of the root confirmed the dominant role of lignin accumulation in silicon-induced drought resistance. The lignin monomer content in the root was enhanced by silicon, and was accompanied by the enhancement of drought resistance. Histochemical and transcriptional analyses of lignin showed that lignin accumulation was promoted by silicon under drought stress. In addition, in the root zone, silicon-induced lignin accumulation increased as the distance from the root tip increased under drought stress. Surprisingly, the Dwarf gene was upregulated by silicon in the roots. Micro Tom Dwarf gene mutation and Micro Tom-d + Dwarf gene functional complementation were further used to confirm that Dwarf regulates the spatial accuracy of SHR expression in the root. Therefore, root lignin accumulation plays a dominant role in silicon-induced drought resistance in tomato and the regulation of spatial accuracy of root lignification by silicon under drought stress is through the BR pathway, thereby avoiding the inhibition of root growth caused by root lignification.

Physiological and molecular mechanism of ginger (Zingiber officinale Roscoe) seedling response to salt stress.

We used 'Shannong No.1' experimental material to simulate higher salt concentration in ginger and analyzed the physiological responses of different parts of ginger seedlings under salt stress. The results showed that salt stress led to a significant decrease in fresh and dry weight of ginger, lipid membrane peroxidation, increased sodium ion content and enhanced activity of antioxidant enzymes. Compared with the control, the overall plant dry weight of ginger under salt stress decreased by about 60%, and the MDA content in roots, stems, leaves, and rhizomes increased by 372.27%, 184.88%, 291.5%, and 171.13%, respectively, and the APX content increased by 188.85%, 165.56%, 195.38%, and 40.08%, respectively. After analysis of the physiological indicators, it was found that the roots and leaves of ginger were the most significantly changed parts. We analyzed the transcriptional differences between ginger roots and leaves by RNA-seq and found that they jointly initiated MAPK signaling pathways in response to salt stress. By combining physiological and molecular indicators, we elucidated the response of different tissues and parts of ginger to salt stress during the seedling stage.

Effects of soil waterlogging and high-temperature stress on photosynthesis and photosystem II of ginger (Zingiber officinale).

Heavy waterlogging and high temperatures occur frequently in North China, yet the effects of changing environments on photochemical reactions and carbon metabolism have not been described in ginger. To determine the impact of waterlogging and high temperature on ginger, in this study, treatment groups were established as follows: (a) well-watered at ambient temperature (28 °C/22 °C) (CK), (b) well-watered at moderate temperature (33 °C/27 °C) (MT), (c) well-watered at high temperature (38 °C/32 °C) (HT), (d) waterlogging at ambient temperature (CK-WL), (e) waterlogging at moderate temperature (MT-WL), and (f) waterlogging at high temperature (HT-WL) during the rhizome growth period. We analyzed the effect of different treatments on the photosynthetic performance of ginger. Here, our results showed that waterlogging and high temperature irreversibly decreased the photosynthetic pigment content, increased the ROS content of leaves, inhibited leaf carbon assimilation and limited PSII electron transport efficiency. In addition, waterlogging in isolation and high temperature in isolation affected photosynthesis to varying degrees. Taken together, photosynthesis was more sensitive to the combined stress than to the single stresses. The results of this research provide deep insights into the response mechanisms of crop photosynthesis to different water and temperature conditions and aid the development of scientific methods for mitigating plant damage over time.

Effect of ofloxacin levels on growth, photosynthesis and chlorophyll fluorescence kinetics in tomato.

Antibiotic pollution has become a global environmental pollution problem. Chlorophyll fluorescence is one of the most important indicators reflecting the degree to which plants are influenced by the environment. Ofloxacin (OFL) is a highly toxic antibiotic pollutant, and there are few reports on the effects of changes in OFL levels on tomato chlorophyll fluorescence parameters. In this study, we investigated the responses of tomato growth, photosynthetic activity and chlorophyll fluorescence kinetics to exogenous OFL exposure (as the concentrations of 0, 2.5, 5, 10 and 20 mg L-1). The results showed that lower concentrations of OFL (2.5 mg L-1) had little impact on tomato growth, while plant growth was inhibited with the OFL concentration increasing. At higher OFL concentrations (5, 10 and 20 mg L-1), chloroplasts ruptured, and chlorophyll became degraded, resulting in leaf etiolation. Furthermore, the photosynthetic and photochemical efficiency and electron transfer rate were significantly inhibited by OFL. Moreover, damage to the oxygen-evolving complex on the donor side of PSⅡ prevented electron transfer from QA to QB and led to photoinhibition. In conclusion, higher OFL concentration reduced photosynthesis by destroying the photosynthetic mechanism in tomato, resulting in tomato leaf etiolation and plant growth inhibition.

Effect of different LED light quality combination on the content of vitamin C, soluble sugar, organic acids, amino acids, antioxidant capacity and mineral elements in green onion (Allium fistulosum L.).

The effects of blue-white, green-white, yellow-white, and red-white light combinations on the nutrient composition and antioxidant capacity of pseudo-stems and leaves of 'Yuanzang' green onion were investigated using light-emitting diodes (LEDs) with precise modulation of light quality, using white light as the control. The results showed that the leaf pigment, vitamin C, soluble sugar, organic acids, free amino acids, mineral elements, and antioxidant levels were significantly higher in green onion under blue-white combined light treatment, followed by white and red-white combined light, while green-white and yellow-white combined light significantly reduced fruit quality and antioxidant capacity. In conclusion, supplementation with blue LED light was the most effective light condition to improve palatability, nutritional value, and storage resistance of green onion by enhancing various nutrients in the plants, increasing antioxidant levels, and delaying plant aging.

A hydrogen sulfide application can alleviate the toxic effects of cadmium on ginger (Zingiber officinale Roscoe).

Cadmium (Cd) is a poisonous element for human health. This study was conducted to explore whether H2S can alleviate the toxic effects of Cd on ginger. Specifically, ginger plants were grown in soil and treated with 7.5 mg·l-1 CdCl2, after which water (T1), 0.8 mM NaHS (T2), or 0.8 mM NaHS and 0.15 mM HT (T3) were added to the soil. The application of NaHS increased the activities of antioxidant enzymes (APX, GR, MDHAR, and DHAR) during the early treatment stage. It also inhibited the decrease in Pn, Gs, and Ls under Cd stress conditions while also limiting the increase in Ci. An analysis of the expression of Cd uptake-related genes indicated that NaHS upregulated the expression of ZoNramp1, which encodes a metal transporter, in roots as well as ZoPCS1, which encodes a phytochelatin synthase. In contrast, NaHS downregulated ZoHMA2 expression in the rhizomes and roots under Cd stress conditions.

Comprehensive insights into the influence of supplemental green light on the photosynthesis of ginger (Zingiber officinale Roscoe).

Although green light is not considered to contribute to the photosynthesis of plants, the photosynthesis of ginger, a dual-purpose vegetable used as a medicine and food, is affected by the green wave band. In this study, the supplementary green band of sunlight (SG) increased the net photosynthetic rate (Pn), maximal photochemical efficiency of PSII (Fv/Fm), and actual photochemical efficiency of PSII (Y(II)) compared with the sunlight treatment (S). The Pn and Fv/Fm of the SG treatment were higher than those of the white light (W) treatment, while the Pn and Fv/Fm of the green light (G) treatment alone were lower than those of the W treatment. Further analysis found that the minimal fluorescence (Fo) of the S treatment increased, especially at noon, while the Fo of the SG treatment decreased. Similarly, the Fo of the W treatment increased significantly, while the Fo of the white-green mixed light (WG) treatment decreased. The relative fluorescence values of the K-J-I bands in the SG and WG treatments were lower than those in the S and W treatments, respectively. The photochemical quenching (qP) of the WG treatment was higher than that of the W treatment, while the primary thermal losses corresponded to the sum of nonregulated heat dissipation and fluorescence emission (Y(NO)) of the WG treatment was lower than that of the W treatment. The SG treatment reduced the accumulation of plastoglobules but increased the accumulation of starch granules and leaf thickness. Moreover, the green band supplemented with white light significantly increased the biomass of the aboveground plant parts and promoted the active growth of the aboveground parts. Supplementing green light plays a regulatory role in ginger based on the following four points. First, it effectively promotes the transfer of electrons between the acceptor side of photosystem II; second, it optimizes ginger photosynthesis; third, it alleviates strong light stress by reducing the accumulation of reactive oxygen species; and fourth, it promotes heat dissipation and reduces the rapid burst of active oxygen in the chloroplast caused by excess energy. In summary, green light can significantly optimize the photosynthetic characteristics of ginger.

Silicon enhanced the resistance of Chinese cabbage (Brassica rapa L. ssp. pekinensis) to ofloxacin on the growth, photosynthetic characteristics and antioxidant system.

The negative impact of the misuse of antibiotics on agriculture and human health has become a popular research topic with the increasing usage of antibiotics; however, little information is available about the mechanisms of OFL (ofloxacin) and Si (silicon). In this experiment, we applied 7 OFL concentrations to two Chinese cabbage cultivars (Qinghua and Biyu) to screen proper OFL concentrations. OFL concentrations of 0, 1, 2.5 and 5 mg L-1 were selected for the subsequent test and 1.2 mmol L-1 Si was used as mitigation. The results showed that Biyu suffered more damage than Qinghua and the injury degree increased in a concentration-dependent manner. With increasing OFL concentrations, the photosynthetic fluorescence was weakened significantly; under 1, 2.5 and 5 mg L-1 OFL, the Pn reduced by 5.35%, 35.92% and 43.62% in Qinghua and 33.98%, 41.94% and 64.66% in Biyu, respectively. The production rate of O2-, H2O2 and the MDA content were increased and Biyu appeared higher increase rates. In addition, the antioxidant enzymes contents first increased and then decreased and that of Qinghua increased more than Biyu. Si ensured the growth under OFL and protected its photosynthetic ability. Under the OFL1+Si, OFL2.5 + Si and OFL5+Si treatments, Pn increased by 3.91%, 15.95 and 15.69% in Qinghua and 28.82%, 20.40% and 39.01% in Biyu. Si also maintained the structural integrity of leaf organelles and improved the scavenging ability of ROS by increasing the activity and relative gene expression of antioxidant enzymes. Moreover, varietal differences may play a more important role than Si.

Root morphology ion absorption and antioxidative defense system of two Chinese cabbage cultivars (Brassica rapa L.) reveal the different adaptation mechanisms to salt and alkali stress.

Salt stress and alkali stress are major factors that affect the growth and production of Chinese cabbage. To explore their tolerant mechanism to salt and alkali stress, three salinity levels (0, 50, 100 mmol/L NaCl) and three different pH levels (pH6.5, pH7.5, pH8.5) were interactively applied on Qinghua (salt-tolerant-alkali-sensitive) and Biyu (salt-sensitive-alkali-tolerant) cultivars; the root morphology, ion content and antioxidant enzymes were determined. The results showed that the root morphology and root water content of Qinghua under S0pH7.5 and S0pH8.5 were seriously affected, and the content of H2O2 and MDA increased by 143%, 190% and 234%, 294%, respectively, compared with S0pH6.5; when Biyu was under S50pH6.5 and S100pH6.5 stress, the content of H2O2 and MDA increase to 152%, 208% and to 240%, 263%, respectively, but the activities and genes expression of SOD, POD, CAT, AAO, APX, DHAR and MDHAR did not change. The root and the contents of H2O2 and MDA were not affected when Qinghua was treated with salt and Biyu was treated with alkali, but the activities of the antioxidant enzymes increased to 150-200%, and their relative expression was overexpressed and 2.5-3.5-fold of the S0pH6.5. The increase of Na+ in Qinghua was limited under salt stress, Mg2+ in Biyu increased significantly under alkali stress. These all indicated that the adaptability of roots could reflect the degree of tolerance; Chinese cabbage with high salt and alkali tolerance enhanced the regulation of their absorption of ions and increased the relative expression and activities of related antioxidant enzymes.

Different responses of two Chinese cabbage (Brassica rapa L. ssp. pekinensis) cultivars in photosynthetic characteristics and chloroplast ultrastructure to salt and alkali stress.

MAIN CONCLUSION: Salt and alkali stress affected the photosynthetic characteristics of Chinese cabbages. A salt-tolerant cultivar maintained its tolerance by ensuring the high ability of photosynthesis. The synthesis of organic acids and carbohydrates in leaves played important roles in improving the photosynthetic capacity of alkali-tolerant plants. Soil salinization has become an increasingly serious ecological problem, which limits the quality and yield of crops. As an important economic vegetable in winter, however, little is known about the response of Chinese cabbage to salt, alkali and salt-alkali stress in photosynthetic characteristics and chloroplast ultrastructure. Thus, two Chinese cabbage cultivars, 'Qinghua' (salt-tolerant-alkali-sensitive) and 'Biyu' (salt-sensitive-alkali-tolerant) were investigated under stresses to clarify the similarities and differences between salt tolerance and alkali tolerance pathways in Chinese cabbage. We found that the root of Qinghua, the leaf ultrastructure and net photosynthetic rate (Pn), stomatal conductance (Gs), water use efficiency (WUE), maximum photochemical quantum yield of PSII (Fv/Fm) and nonphotochemical quenching (NPQ) were not affected by salt stress. However, Biyu was seriously affected under salt stress. Its growth indexes decreased by between 60 and 30% compared with the control and the photosynthetic indexes were also seriously affected under salt stress. This indicated that the salt-tolerant cultivar Qinghua improved the photosynthetic fluorescence ability to promote the synthesis of organic matter resulting in salt tolerance. In contrast, under alkali treatment, the root of Biyu was affected by alkali stress, but could still maintain good growth, and root and leaf structure were not seriously affected and could maintain the normal operations. Biyu improved its tolerance by improving the water use efficiency, regulating the synthesis of organic acids and carbohydrates, ensuring the synthesis of organic matter and ensured the normal growth of the plant.

Comparative Transcriptome Analysis of Two Contrasting Chinese Cabbage (Brassica rapa L.) Genotypes Reveals That Ion Homeostasis Is a Crucial Biological Pathway Involved in the Rapid Adaptive Response to Salt Stress.

Salt is the most important limiting factor in plant yield and quality. Different Chinese cabbage cultivars appeared different salt tolerances, but there are few studies attempting to elucidate the mechanism underlying this phenomenon. In this study, 100 mmol L-1 NaCl was found to be the most suitable treatment concentration according to a sprouting bag test of 39 Chinese cabbage cultivars, and through comprehensive comparison and analysis, the relative values of fresh weight and electrolyte leakage in leaves proved to be convenient indicators for the identification of salt tolerance in Chinese cabbage. We analyzed the physiological responses of Qinghua45 (salt-tolerant) and Biyuchunhua (salt-sensitive) in terms of the growth indexes, ion homeostasis and Photosynthesis, the results indicated that Qinghua45 could ensure osmotic regulation, ion homeostasis and photosynthesis under salt stress. Next, we compared the transcriptome dynamics of the two cultivars. Overall, 2,859 differentially expressed genes (DEGs) were identified, and the number of DEGs in Qinghua45 was significantly less than that in Biyuchunhua. VDAC promoted the release of Ca2+, which indirectly promoted the transport of Na+ to vacuoles through the SOS2 pathway. Cation/H (+) antiporter 17 and V-H + -ATPase improve the exchange of Na+ and H+ and maintain Na+ in the vacuoles, thereby reducing the injury affected by salt stress. Increases in galactinol synthase and soluble protein synthesis helped relieve osmotic stress caused by salt, together, they regulated the Na+ content and chlorophyll biosynthesis of the plant and enabled the plant to adapt to salt stress over time.

The Spectral Irradiance, Growth, Photosynthetic Characteristics, Antioxidant System, and Nutritional Status of Green Onion (Allium fistulosum L.) Grown Under Different Photo-Selective Nets.

The active regulation of the plant growth environment is a common method for optimizing plant yield and quality. In horticulture today, light quality control is carried out using photo-selective nets or membranes to improve the yield and quality of cultivated plants. In the present study, with natural light as the control (CK), we tested different photo-selective nets (white, WN; blue, BN; green, GN; yellow, YN; and red, RN) with 30% shade for characteristics of growth, development, quality, yield, photosynthesis, and chlorophyll fluorescence, considering the antioxidant system, as well as the influence of element absorption and transformation of green onion (Allium fistulosum L.) plants at different growth stages. We found that plants under BN and WN have greater height and fresh weight than those of plants under the other nets. Plants under the BN treatment had the highest quality, yield, photosynthetic pigment content, net photosynthetic rate, transpiration rate, and stomatal conductance, whereas the intercellular CO2 concentration was the highest in plants in the YN treatment. The photosynthesis noon break phenomenon was significantly lower in plants with covered photo-selective nets than in CK plants. NPQ was the highest in the YN treatment, and Fv/Fm, ΦPSII, and qP among the plants in the other treatments were different; from highest to lowest, they were as follows: BN > WN > CK > RN > GN > YN. The active oxygen content of green onion leaves in the BN treatment was significantly lower than that in the other treatments, and their key enzyme activity was significantly increased. BN also improved the absorption and transformation of elements in various organs of green onion.

Response of growth, photosynthetic electron transfer, and chloroplast ultrastructure to different LED light combination in green onion (Allium fistulosum L.).

With the rapid development of facility agriculture, it has become popular to study the influences of different light qualities on the growth, material metabolism, and morphology of horticultural crops. Last several years, green onions cultivation models have undergone major changes, and facility cultivation has developed rapidly. To determine the impact of light quality on the green onions, we studied the parameters connected to photosynthesis, incorporating growth, and development, photosynthetic rate (Pn ), chlorophyll fluorescence, light response curve, photosynthetic electron transfer, and chloroplast ultrastructure. We roundly analyzed the influences of different LED light combination (white: W, white-blue combination 3:1:WB, white-green combination 3:1:WG, white-yellow combination 3:1:WY, and white-red combination 3:1:WR, light intensity: 500 ± 10 µmol photons m-2 s-1 ) on the photosynthetic performance of green onions. The WB light led to better results than those of the WR, WG, and WY. There were significant performance improvements in leaf area, plant height, stem thickness, relative growth rate (RGR), pigment content, photosynthetic capacity, photosynthetic electron transfer efficiency, and chloroplast ultrastructure integrity. In contrast, plants treated with WG and WY were exposed to appreciably blocked light, but they effectively formed a light protection mechanism. The results of this research not only provided insight into the response mechanism of crop photosynthesis to different light qualities, but they also provided a scientific foundation for better planting green onions.

Low pH alleviated salinity stress of ginger seedlings by enhancing photosynthesis, fluorescence, and mineral element contents.

We investigated the effects of low pH on the photosynthesis, chlorophyll fluorescence, and mineral contents of the leaves of ginger plants under salt stress. This experiment involved four treatments: T1 (pH 6, 0 salinity), T2 (pH 4, 0 salinity), T3 (pH 6, 100 mmol L-1 salinity) and T4 (pH 4, 100 mmol L-1 salinity). This study showed that photosynthesis (Pn, Gs, WUE and Tr) and chlorophyll fluorescence (qP, Φ PSII, and Fv/Fm) significantly decreased under salt stress; however, all the parameters of the ginger plants under the low-pH treatment and salt stress recovered. Moreover, low pH reduced the content of Na and enhanced the contents of K, Mg, Fe and Zn in the leaves of ginger plants under salt stress. Taken together, these results suggest that low pH improves photosynthesis efficiency and nutrient acquisition and reduces the absorption of Na, which could enhance the salt tolerance of ginger.

Comparison of the effects of LED light quality combination on growth and nutrient accumulation in green onion (Allium fistulosum L.).

The growth and development and metabolism of plants have different physiological responses to different light qualities. To study the influence of light qualities on green onions, the impacts of LED light treatment on the growth and development as well as the nutritional components and flavor substances in green onions were studied under controlled conditions. Leaf area, plant height, dry matter accumulation, Dickson's quality index (DQI), nutritional content, and volatile compounds under different light quality treatments were determined. The results indicated that the white and blue combined light (W/B: 3/1) treatment was the most beneficial to growth and nutrient accumulation and led to higher levels of sulfur compounds in the green onions than the other treatments. This shows that it is possible to control the contents of compounds that affect consumer preferences by adjusting the lighting conditions and to thereby increase the value and quality of seasoning vegetables.

Effect of a mist culture system on photosynthesis and nitrogen metabolism in ginger.

To evaluate the transition from traditional shading cultivation to mist cultivation, a field experiment was carried out. The results demonstrated that compared with traditional shading, the mist treatment significantly reduced leaf temperature. Likewise, the higher transpiration rate also contributes to reducing leaf temperature and protects ginger from heat stress in summer. Moreover, a higher instantaneous efficiency of water use suggested that water lost via transpiration was beneficial under a mist culture system. The higher instantaneous efficiency of water use in the mist treatment was caused mainly by the higher net photosynthetic rate, which is further reflected by the higher rhizome yield of ginger under the mist culture system. Instead of lowering the temperature by lowering photon flux density, mist treatment does not seriously reduce the photon flux density while reducing the temperature of the blade. Hence, the net photosynthetic rate in the shading treatment is significantly lower than that in the mist treatment, although the maximal quantum yield of photosystem II and the actual photochemical efficiency of photosystem II in ginger in the shading treatment were significantly higher than those in the mist treatment. Lower superoxide anion, hydrogen peroxide, and malondialdehyde contents were also found after mist treatment. Lower ammonium avoids the potential risk of ammonium toxicity and is based on higher nitrate reductase, glutamine synthetase, and glutamate synthase activity but lower glutamate dehydrogenase activity. Therefore, the mist cultivation system improved the physiological characteristics and yields of ginger and can be suggested as an alternative, sustainable, and cleaner cultivation measure.

Photosynthetic characteristics and chloroplast ultrastructure of welsh onion (Allium fistulosum L.) grown under different LED wavelengths.

BACKGROUND: The optimized illumination of plants using light-emitting diodes (LEDs) is beneficial to their photosynthetic performance, and in recent years, LEDs have been widely used in horticultural facilities. However, there are significant differences in the responses of different crops to different wavelengths of light. Thus, the influence of artificial light on photosynthesis requires further investigation to provide theoretical guidelines for the light environments used in industrial crop production. In this study, we tested the effects of different LEDs (white, W; blue, B; green, G; yellow, Y; and red, R) with the same photon flux density (300 µmol/m2·s) on the growth, development, photosynthesis, chlorophyll fluorescence characteristics, leaf structure, and chloroplast ultrastructure of Welsh onion (Allium fistulosum L.) plants. RESULTS: Plants in the W and B treatments had significantly higher height, leaf area, and fresh weight than those in the other treatments. The photosynthetic pigment content and net photosynthetic rate (Pn) in the W treatment were significantly higher than those in the monochromatic light treatments, the transpiration rate (E) and stomatal conductance (Gs) were the highest in the B treatment, and the intercellular CO2 concentration (Ci) was the highest in the Y treatment. The non-photochemical quenching coefficient (NPQ) was the highest in the Y treatment, but the other chlorophyll fluorescence characteristics differed among treatments in the following order: W > B > R > G > Y. This includes the maximum photochemical efficiency of photosystem II (PSII) under dark adaptation (Fv/Fm), maximum photochemical efficiency of PSII under light adaptation (Fv'/Fm'), photochemical quenching coefficient (qP), actual photochemical efficiency (ΦPSII), and apparent electron transport rate (ETR). Finally, the leaf structure and chloroplast ultrastructure showed the most complete development in the B treatment. CONCLUSIONS: White and blue light significantly improved the photosynthetic efficiency of Welsh onions, whereas yellow light reduced the photosynthetic efficiency.

Low pH altered salt stress in antioxidant metabolism and nitrogen assimilation in ginger (Zingiber officinale) seedlings.

The effects of low pH on antioxidant metabolism and nitrogen (N) assimilation in ginger seedlings under salt stress were investigated. A two-way randomized block design was used: the main treatment consisted of two pH levels, normal and low pH (6.0 and 4.0, respectively), and the other treatment consisted of two salinity levels, 0 and 100 mmol l-1 Na+ (NaCl and Na2 SO4 ). The results showed that low pH decreased the malondialdehyde (MDA) and hydrogen peroxide contents of ginger seedling leaves under salt stress. Moreover, low pH and salt stress significantly decreased the contents of non-enzymatic antioxidants, including ascorbate (AsA) and glutathione (GSH), and increased the activities of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR) and glutathione reductase (GR). In addition, salt stress inhibited the N assimilation process in ginger seedling leaves, but low pH improved N assimilation under salt stress. Our finding was that low pH alleviated oxidative damage and promoted N assimilation under salt stress.