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1.
Mol Biol Cell ; 35(4): ar48, 2024 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-38335450

RESUMEN

Nuclear envelope reassembly during the final stages of each mitosis depends on disassembling spindle microtubules without disrupting chromosome separation. This process involves the transient recruitment of the ESCRT-III complex and spastin, a microtubule-severing AAA (ATPases associated with diverse cellular activities) mechanoenzyme, to late-anaphase chromosomes. However, dissecting mechanisms underlying these rapid processes, which can be completed within minutes, has been difficult. Here, we combine fast-acting chemical inhibitors with live-cell imaging and find that spindle microtubules, along with spastin activity, regulate the number and lifetimes of spastin foci at anaphase chromosomes. Unexpectedly, spastin inhibition impedes chromosome separation, but does not alter the anaphase localization dynamics of CHMP4B, an ESCRT-III protein, or increase γ-H2AX foci, a DNA damage marker. We show spastin inhibition increases the frequency of lamin-lined nuclear microtunnels that can include microtubules penetrating the nucleus. Our findings suggest failure to sever spindle microtubules impedes chromosome separation, yet reforming nuclear envelopes can topologically accommodate persistent microtubules ensuring nuclear DNA is not damaged or exposed to cytoplasm.


Asunto(s)
Anafase , Microtúbulos , Espastina/metabolismo , Microtúbulos/metabolismo , Cromosomas/metabolismo , Complejos de Clasificación Endosomal Requeridos para el Transporte/metabolismo
2.
Proc Natl Acad Sci U S A ; 119(39): e2204068119, 2022 09 27.
Artículo en Inglés | MEDLINE | ID: mdl-36122237

RESUMEN

Chromosome segregation requires load-bearing interactions across kinetochore fibers and antiparallel microtubule bundles, which constitute the spindle midzone. Mechanical properties of kinetochore fibers have been characterized during metaphase, when the mitotic spindle achieves steady state. However, it has been difficult to probe the mechanics of the spindle midzone that elongates during anaphase. Here, we combine superresolution expansion and electron microscopies, lattice light-sheet imaging, and laser microsurgery to examine how midzone organization sets its mechanics. We find that individual midzone bundles extend out to multiple positions across chromosomes and form multiple apparent microtubule-based connections with each other. Across the spindle's short axis, these microtubule bundles exhibit restricted, submicrometer-amplitude motions, which are weakly correlated on <10s timescales. Severing individual midzone bundles near their center does not substantially affect positions of neighboring bundles, nor the overall structural stability of the midzone. In contrast, severing multiple midzone bundles or individual bundles at their chromosome-proximal ends significantly displaces neighboring microtubule bundles. Together, these data suggest a model wherein multiple midzone connections both reinforce its structure and mechanically isolate individual bundles from local perturbations. This feature sets the robust midzone architecture to accommodate disruptions, including those which result from lagging chromosomes, and achieve stereotypic outputs, such as proper chromosome separation.


Asunto(s)
Anafase , Huso Acromático , Segregación Cromosómica , Cinetocoros , Microtúbulos
3.
Curr Biol ; 32(5): 1049-1063.e4, 2022 03 14.
Artículo en Inglés | MEDLINE | ID: mdl-35108523

RESUMEN

Proper segregation of chromosomes during mitosis depends on "amphitelic attachments"-load-bearing connections of sister kinetochores to the opposite spindle poles via bundles of microtubules, termed as the "K-fibers." Current models of spindle assembly assume that K-fibers arise largely from stochastic capture of microtubules, which occurs at random times and locations and independently at sister kinetochores. We test this assumption by following the movements of all kinetochores in human cells and determine that most amphitelic attachments form synchronously at a specific stage of spindle assembly and within a spatially distinct domain. This biorientation domain is enriched in bundles of antiparallel microtubules, and perturbation of microtubule bundling changes the temporal and spatial dynamics of amphitelic attachment formation. Structural analyses indicate that interactions of kinetochores with microtubule bundles are mediated by non-centrosomal short microtubules that emanate from most kinetochores during early prometaphase. Computational analyses suggest that momentous molecular motor-driven interactions with antiparallel bundles rapidly convert these short microtubules into nascent K-fibers. Thus, load-bearing connections to the opposite spindle poles form simultaneously on sister kinetochores. In contrast to the uncoordinated sequential attachments of sister kinetochores expected in stochastic models of spindle assembly, our model envisions the formation of amphitelic attachments as a deterministic process in which the chromosomes connect with the spindle poles synchronously at a specific stage of spindle assembly and at a defined location determined by the spindle architecture. Experimental analyses of changes in the kinetochore behavior in cells with perturbed activity of molecular motors CenpE and dynein confirm the predictive power of the model.


Asunto(s)
Cinetocoros , Huso Acromático , Segregación Cromosómica , Humanos , Microtúbulos , Mitosis
4.
Cell Rep ; 35(2): 108947, 2021 04 13.
Artículo en Inglés | MEDLINE | ID: mdl-33852852

RESUMEN

During mitochondrial fission, key molecular and cellular factors assemble on the outer mitochondrial membrane, where they coordinate to generate constriction. Constriction sites can eventually divide or reverse upon disassembly of the machinery. However, a role for membrane tension in mitochondrial fission, although speculated, has remained undefined. We capture the dynamics of constricting mitochondria in mammalian cells using live-cell structured illumination microscopy (SIM). By analyzing the diameters of tubules that emerge from mitochondria and implementing a fluorescence lifetime-based mitochondrial membrane tension sensor, we discover that mitochondria are indeed under tension. Under perturbations that reduce mitochondrial tension, constrictions initiate at the same rate, but are less likely to divide. We propose a model based on our estimates of mitochondrial membrane tension and bending energy in living cells which accounts for the observed probability distribution for mitochondrial constrictions to divide.


Asunto(s)
Citoesqueleto/metabolismo , Mitocondrias/metabolismo , Dinámicas Mitocondriales/fisiología , Membranas Mitocondriales/metabolismo , Animales , Fenómenos Biomecánicos , Células COS , Chlorocebus aethiops , Citoesqueleto/ultraestructura , Dinaminas/genética , Dinaminas/metabolismo , Complejo IV de Transporte de Electrones/genética , Complejo IV de Transporte de Electrones/metabolismo , Expresión Génica , Genes Reporteros , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Humanos , Proteínas Luminiscentes/genética , Proteínas Luminiscentes/metabolismo , Mitocondrias/ultraestructura , Membranas Mitocondriales/ultraestructura , Tensión Superficial , Transfección , Transgenes , Proteína Fluorescente Roja
5.
Dev Cell ; 54(5): 574-582.e4, 2020 09 14.
Artículo en Inglés | MEDLINE | ID: mdl-32818469

RESUMEN

Mesoscale macromolecular complexes and organelles, tens to hundreds of nanometers in size, crowd the eukaryotic cytoplasm. It is therefore unclear how mesoscale particles remain sufficiently mobile to regulate dynamic processes such as cell division. Here, we study mobility across dividing cells that contain densely packed, dynamic microtubules, comprising the metaphase spindle. In dividing human cells, we tracked 40 nm genetically encoded multimeric nanoparticles (GEMs), whose sizes are commensurate with the inter-filament spacing in metaphase spindles. Unexpectedly, the effective diffusivity of GEMs was similar inside the dense metaphase spindle and the surrounding cytoplasm. Eliminating microtubules or perturbing their polymerization dynamics decreased diffusivity by ~30%, suggesting that microtubule polymerization enhances random displacements to amplify diffusive-like motion. Our results suggest that microtubules effectively fluidize the mitotic cytoplasm to equalize mesoscale mobility across a densely packed, dynamic, non-uniform environment, thus spatially maintaining a key biophysical parameter that impacts biochemistry, ranging from metabolism to the nucleation of cytoskeletal filaments.


Asunto(s)
División Celular/fisiología , Citoplasma/metabolismo , Metafase/fisiología , Microtúbulos/metabolismo , Humanos , Nanopartículas/metabolismo , Orgánulos/metabolismo , Huso Acromático/fisiología
6.
J Cell Biol ; 218(8): 2529-2544, 2019 08 05.
Artículo en Inglés | MEDLINE | ID: mdl-31248912

RESUMEN

In the spindle midzone, microtubules from opposite half-spindles form bundles between segregating chromosomes. Microtubule bundles can either push or restrict chromosome movement during anaphase in different cellular contexts, but how these activities are achieved remains poorly understood. Here, we use high-resolution live-cell imaging to analyze individual microtubule bundles, growing filaments, and chromosome movement in dividing human cells. Within bundles, filament overlap length marked by the cross-linking protein PRC1 decreases during anaphase as chromosome segregation slows. Filament ends within microtubule bundles appear capped despite dynamic PRC1 turnover and submicrometer proximity to growing microtubules. Chromosome segregation distance and rate are increased in two human cell lines when microtubule bundle assembly is prevented via PRC1 knockdown. Upon expressing a mutant PRC1 with reduced microtubule affinity, bundles assemble but chromosome hypersegregation is still observed. We propose that microtubule overlap length reduction, typically linked to pushing forces generated within filament bundles, is needed to properly restrict spindle elongation and position chromosomes within daughter cells.


Asunto(s)
Cromosomas Humanos/metabolismo , Imagenología Tridimensional , Movimiento , Huso Acromático/metabolismo , Anafase , Proteínas de Ciclo Celular/metabolismo , Segregación Cromosómica , Recuperación de Fluorescencia tras Fotoblanqueo , Células HeLa , Humanos , Proteínas Asociadas a Microtúbulos/metabolismo , Microtúbulos/metabolismo , Mutación/genética
7.
Elife ; 82019 05 03.
Artículo en Inglés | MEDLINE | ID: mdl-31050340

RESUMEN

Protein concentration gradients pattern developing organisms and single cells. In Schizosaccharomyces pombe rod-shaped cells, Pom1 kinase forms gradients with maxima at cell poles. Pom1 controls the timing of mitotic entry by inhibiting Cdr2, which forms stable membrane-associated nodes at mid-cell. Pom1 gradients rely on membrane association regulated by a phosphorylation-dephosphorylation cycle and lateral diffusion modulated by clustering. Using quantitative PALM imaging, we find individual Pom1 molecules bind the membrane too transiently to diffuse from pole to mid-cell. Instead, we propose they exchange within longer lived clusters forming the functional gradient unit. An allelic series blocking auto-phosphorylation shows that multi-phosphorylation shapes and buffers the gradient to control mid-cell levels, which represent the critical Cdr2-regulating pool. TIRF imaging of this cortical pool demonstrates more Pom1 overlaps with Cdr2 in short than long cells, consistent with Pom1 inhibition of Cdr2 decreasing with cell growth. Thus, the gradients modulate Pom1 mid-cell levels according to cell size.


Asunto(s)
Citoplasma/enzimología , Proteínas Quinasas/metabolismo , Procesamiento Proteico-Postraduccional , Proteínas de Schizosaccharomyces pombe/metabolismo , Schizosaccharomyces/enzimología , Membrana Celular/metabolismo , Fosforilación , Unión Proteica , Proteínas Quinasas/análisis , Proteínas Serina-Treonina Quinasas/metabolismo , Proteínas de Schizosaccharomyces pombe/análisis
8.
PLoS One ; 10(11): e0142949, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-26600467

RESUMEN

Three-dimensional (3D) localization-based super-resolution microscopy (SR) requires correction of aberrations to accurately represent 3D structure. Here we show how a depth-dependent lateral shift in the apparent position of a fluorescent point source, which we term `wobble`, results in warped 3D SR images and provide a software tool to correct this distortion. This system-specific, lateral shift is typically > 80 nm across an axial range of ~ 1 µm. A theoretical analysis based on phase retrieval data from our microscope suggests that the wobble is caused by non-rotationally symmetric phase and amplitude aberrations in the microscope's pupil function. We then apply our correction to the bacterial cytoskeletal protein FtsZ in live bacteria and demonstrate that the corrected data more accurately represent the true shape of this vertically-oriented ring-like structure. We also include this correction method in a registration procedure for dual-color, 3D SR data and show that it improves target registration error (TRE) at the axial limits over an imaging depth of 1 µm, yielding TRE values of < 20 nm. This work highlights the importance of correcting aberrations in 3D SR to achieve high fidelity between the measurements and the sample.


Asunto(s)
Percepción de Profundidad , Imagenología Tridimensional/métodos , Microscopía Fluorescente/métodos , Proteínas Bacterianas/metabolismo , Calibración , Caulobacter/metabolismo , Proteínas del Citoesqueleto/metabolismo , Rotación
9.
Sci Rep ; 4: 4577, 2014 Apr 03.
Artículo en Inglés | MEDLINE | ID: mdl-24694686

RESUMEN

Super resolution microscopy such as STORM and (F)PALM is now a well known method for biological studies at the nanometer scale. However, conventional imaging schemes based on sparse activation of photo-switchable fluorescent probes have inherently slow temporal resolution which is a serious limitation when investigating live-cell dynamics. Here, we present an algorithm for high-density super-resolution microscopy which combines a sparsity-promoting formulation with a Taylor series approximation of the PSF. Our algorithm is designed to provide unbiased localization on continuous space and high recall rates for high-density imaging, and to have orders-of-magnitude shorter run times compared to previous high-density algorithms. We validated our algorithm on both simulated and experimental data, and demonstrated live-cell imaging with temporal resolution of 2.5 seconds by recovering fast ER dynamics.


Asunto(s)
Algoritmos , Microscopía Fluorescente , Animales , Células COS , Carbocianinas/química , Línea Celular , Chlorocebus aethiops , Retículo Endoplásmico/metabolismo , Colorantes Fluorescentes/química , Humanos , Microtúbulos/química , Microtúbulos/metabolismo , Relación Señal-Ruido
10.
Chemphyschem ; 15(4): 750-5, 2014 Mar 17.
Artículo en Inglés | MEDLINE | ID: mdl-24554553

RESUMEN

Cell-permeable rhodamine dyes are reductively quenched by NaBH4 into a non-fluorescent leuco-rhodamine form. Quenching is reversible, and their fluorescence is recovered when the dyes are oxidized. In living cells, oxidation occurs spontaneously, and can result in up to ten-fold higher densities of single molecule localizations, and more photons per localization as compared with unmodified dyes. These two parameters directly impact the achievable resolution, and we see a significant improvement in the quality of live-cell point-localization super-resolution images taken with reduced dyes. These improvements carry over to increase the density of trajectories for single-molecule tracking experiments.


Asunto(s)
Colorantes Fluorescentes/química , Microscopía Fluorescente/métodos , Rodaminas/química , Borohidruros/química , Supervivencia Celular , Fluorescencia , Humanos , Estructura Molecular , Oxidación-Reducción , Rodaminas/análisis , Células Tumorales Cultivadas
11.
Biomed Opt Express ; 5(11): 3935-48, 2014 Nov 01.
Artículo en Inglés | MEDLINE | ID: mdl-26526603

RESUMEN

Localization microscopy achieves nanoscale spatial resolution by iterative localization of sparsely activated molecules, which generally leads to a long acquisition time. By implementing advanced algorithms to treat overlapping point spread functions (PSFs), imaging of densely activated molecules can improve the limited temporal resolution, as has been well demonstrated in two-dimensional imaging. However, three-dimensional (3D) localization of high-density data remains challenging since PSFs are far more similar along the axial dimension than the lateral dimensions. Here, we present a new, high-density 3D imaging system and algorithm. The hybrid system is implemented by combining astigmatic and biplane imaging. The proposed 3D reconstruction algorithm is extended from our state-of-the art 2D high-density localization algorithm. Using mutual coherence analysis of model PSFs, we validated that the hybrid system is more suitable than astigmatic or biplane imaging alone for 3D localization of high-density data. The efficacy of the proposed method was confirmed via simulation and real data of microtubules. Furthermore, we also successfully demonstrated fluorescent-protein-based live cell 3D localization microscopy with a temporal resolution of just 3 seconds, capturing fast dynamics of the endoplasmic recticulum.

12.
ACS Chem Biol ; 8(12): 2643-8, 2013 Dec 20.
Artículo en Inglés | MEDLINE | ID: mdl-24079385

RESUMEN

Point localization super-resolution imaging (SR) requires dyes that can cycle between fluorescent and dark states, in order for their molecular positions to be localized and create a reconstructed image. Dyes should also densely decorate biological features of interest to fully reveal structures being imaged. We tested site-specific dyes in several live-cell compatible imaging media and evaluated their performance in situ. We identify a number of new dyes and imaging medium-dye combinations for live staining, that densely highlight intracellular structures with excellent photophysical performance for SR.


Asunto(s)
Colorantes Fluorescentes/química , Imagen Molecular/métodos , Coloración y Etiquetado/métodos , Línea Celular Tumoral , Medios de Cultivo/química , Retículo Endoplásmico/ultraestructura , Colorantes Fluorescentes/metabolismo , Aparato de Golgi/ultraestructura , Humanos , Lisosomas/ultraestructura , Microscopía Fluorescente , Mitocondrias/ultraestructura
13.
Phys Chem Chem Phys ; 15(25): 10418-28, 2013 Jul 07.
Artículo en Inglés | MEDLINE | ID: mdl-23681155

RESUMEN

The small thiol ß-mercaptoethanol (BME) has been used as an anti-blinking reagent for CdSe/ZnS quantum dots (QDs), although its effects on QD photoluminescence are complex. It acts as an antioxidant as well as a hole scavenger on both CdSe and CdTe, which leads to changes in emission intensity and lifetime that vary qualitatively according to BME concentration, time of incubation, and pH of the solution. Because the band edge energies of InP/ZnS are shifted from those of CdTe and CdSe, it may be expected that thiols including BME might be unable to trap holes from these QDs. In this study, we use steady-state and time-resolved emission spectroscopy with physical fitting models combined with blinking analysis to compare the effects of different concentrations of BME on CdSe/ZnS vs. InP/ZnS QDs over time. We also find excellent correspondence between simple physical model parameters and blinking off times, a finding that will be useful for all blinking studies involving semiconductor nanoparticles. BME alters blinking in InP/ZnS QDs with a single ZnS shell, but not those with double thickness shells. The effects are similar to those seen with CdSe/ZnS, despite very different effects of BME on steady-state spectra, and highly pH-dependent.


Asunto(s)
Compuestos de Cadmio/química , Mercaptoetanol/química , Puntos Cuánticos , Compuestos de Selenio/química , Sulfuros/química , Compuestos de Zinc/química , Concentración de Iones de Hidrógeno
14.
Chem Commun (Camb) ; 49(17): 1714-6, 2013 Feb 28.
Artículo en Inglés | MEDLINE | ID: mdl-23344112

RESUMEN

Carboxylate-terminated and dopamine-conjugated CdSe-ZnS quantum dots (QDs) are imaged in living fibroblasts using fluorescence lifetime imaging microscopy. Changes in lifetime are observed as the QDs are processed in the cells, and are consistent with lifetime measurements in bulk solution using buffers compositions that correspond to different cellular regions.


Asunto(s)
Microscopía Fluorescente , Puntos Cuánticos , Semiconductores , Animales , Ácidos Carboxílicos/química , Dopamina/química , Luz , Ratones , Células 3T3 NIH , Oxidación-Reducción
15.
Nanoscale ; 3(6): 2552-9, 2011 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-21509403

RESUMEN

Indium phosphide (InP) quantum dots (QDs) have emerged as a presumably less hazardous alternative to cadmium-based particles, but their cytotoxicity has not been well examined. Although their constituent elements are of very low toxicity to cells in culture, they nonetheless exhibit phototoxicity related to generation of reactive oxygen species by excited electrons and/or holes interacting with water and molecular oxygen. Using spin-trap electron paramagnetic resonance (EPR) spectroscopy and reporter assays, we find a considerable amount of superoxide and a small amount of hydroxyl radical formed under visible illumination of biocompatible InP QDs with a single ZnS shell, comparable to what is seen with CdTe. A double thickness shell reduces the reactive oxygen species concentration approximately two-fold. Survival assays in five cell lines correspondingly indicate a distinct reduction in toxicity with the double-shell InP QDs. Toxicity varies significantly across cell lines according to the efficiency of uptake, being overall significantly less than what is seen with CdTe or CdSe/ZnS. This indicates that InP QDs are a useful alternative to cadmium-containing QDs, while remaining capable of electron-transfer processes that may be undesirable or which may be exploited for photosensitization applications.


Asunto(s)
Radical Hidroxilo/metabolismo , Indio/farmacología , Fosfinas/farmacología , Puntos Cuánticos , Sulfuros/farmacología , Superóxidos/metabolismo , Compuestos de Zinc/farmacología , Animales , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Humanos , Radical Hidroxilo/química , Indio/química , Ratones , Células 3T3 NIH , Oxidación-Reducción , Fosfinas/química , Rodaminas/química , Rodaminas/farmacología , Sulfuros/química , Superóxidos/química , Compuestos de Zinc/química
16.
Phys Chem Chem Phys ; 11(21): 4298-310, 2009 Jun 07.
Artículo en Inglés | MEDLINE | ID: mdl-19458832

RESUMEN

The response of water-soluble, mercaptocarboxylic acid-capped fluorescent semiconductor nanoparticles, or quantum dots (QDs), to extended visible-light irradiation is variable and poorly described. Here we use time-resolved spectroscopy to investigate the photoluminescence intensities and lifetimes of CdSe/ZnS and CdTe QDs as a function of blue light illumination. Conjugates of the particles to the electron donor dopamine were also investigated, and the effect of the antioxidant beta-mercaptoethanol was explored. Both types of QD showed signs of direct electron transfer to the conjugate, but enhancement was much more pronounced in CdSe/ZnS. A model of the two different types of enhancement is proposed.


Asunto(s)
Compuestos de Cadmio/química , Dopamina/química , Puntos Cuánticos , Compuestos de Selenio/química , Sulfuros/química , Telurio/química , Compuestos de Zinc/química , Luminiscencia , Oxígeno/química , Fotones , Factores de Tiempo
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