RESUMEN
A novel cell-permeable urea-linked dipodal naphthalene-based fluorescent receptor 1 (1,1'-(1,5,5-trimethyl-3-oxocyclohexane-1,2-diyl)bis(3-(naphthalen-2-yl)urea) was designed and synthesized. The cation recognition ability of 1 was evaluated with a library of metal ions in DMSO/H2O (9:1, v/v). The receptor showed a selective chromogenic and fluorescent turn-off response towards Hg(2+) among the surveyed metal ions. The developed sensor was successfully applied to image intracellular Hg(2+) in living cells and also for the determination of Hg(2+) content in real water samples. Moreover, the DFT calculations were performed to complement the experimental evidences.
Asunto(s)
Colorantes Fluorescentes/química , Membranas Intracelulares/química , Mercurio/análisis , Naftalenos/química , Urea/química , Colorantes Fluorescentes/metabolismo , Células HeLa , Humanos , Membranas Intracelulares/metabolismo , Mercurio/metabolismo , Naftalenos/metabolismo , Urea/metabolismoRESUMEN
The noncyclic 2,2'-[ethane-1,2-diylbis (iminomethanediyl)]diphenol (4) fluorescent receptor bearing two amine and hydroxyl groups have been designed and investigated for their binding properties towards various cations. The fluorescent spectral measurements revealed that receptor 4 is a selective fluorescent sensor for Fe(3+) ions but not for metal ions such as Cr(3+), Mn(2+), Co(2+), Ni(2+), Cu(2+), Zn(2+), Cd(2+), Hg(2+), Pb(2+) and Bi(3+). The binding ability was confirmed with spectroscopic methods and density functional theory calculation (DFT). This straightforward and cost effective receptor provides rapid detection of Fe(3+) ions at concentrations as low as 2.5 µM and expected to be useful to design efficient chemically and biological sensor.
Asunto(s)
Aminas/química , Derivados del Benceno/química , Radical Hidroxilo/química , Hierro/análisis , Modelos Moleculares , Teoría Cuántica , Espectrometría de FluorescenciaRESUMEN
A new fluorescent receptor 1,1'-(4-methylbenzene-1,3-diyl)bis[3-(2-sulfanylphenyl)urea] (1) has been designed and synthesized. The receptor showed excellent selectivity for Fe(3+) in DMSO/H2O (8:2, v/v) solvent system over other commonly coexistent metal ions. The binding constant (Ka) of receptor with Fe(3+) was calculated to be 11,250 M(-1), 12,970 M(-1) and 12,970 M(-1) using Benesi-Hildebrand, Scatchard and Connor plot, respectively. The experimental results have been further supported by the detailed DFT calculations.
Asunto(s)
Hierro/análisis , Técnicas de Sonda Molecular , Sondas Moleculares/química , Colorantes Fluorescentes , Conformación Molecular , Espectrometría de Fluorescencia , Espectrofotometría UltravioletaRESUMEN
Natural resonance theory (NRT) and natural bond orbital (NBO) analysis have been carried out on a simple symmetrical and an unsymmetrical substituted squaraine with a view of understanding the structure of the latter type of squaraines. It is found that there are some fundamental differences in the structure and bonding between these two types of squaraines particularly in the resonance weights and delocalization energies. These differences are expected to reflect in the low energy transitions and charge transfer in these squaraines. To investigate this, the nature of the lowest energy transitions occurring on excitation in unsymmetrical squaraines has been studied using high-level symmetry adapted cluster-configuration interaction method (SAC/SAC-CI) and compared with reported experimental observations. In general the agreement with the experimental data is very good. The transition dipole moment always lies on the pi-backbone and is quite large in magnitude. The ground state dipole moment in some cases does not change in the excited state upon excitation while in some other cases there is a large reduction/enhancement in the magnitude indicative of some charge rearrangement in this direction. Inclusion of the solvent using the IEFPCM model, a slightly better agreement with the experiment is found in some cases. Studies are carried out with a different basis set and it is found that the change in basis set has very little effect on the transition energies. In the case of weak side donor groups attached to the central ring the larger charge transfer to the central acceptor ring in general takes place from the O- atoms of the squarylium moiety while in the case of strong donors the charge transfer from the O- atoms to the central rings drop down. We have not observed any correlation between the charge transfer in the excited state to the central ring from the side donor groups and the lowest energy excitation in the molecules. Reduction of the HOMO-LUMO gap (an indication of increase of the diradicaloid character) always leads to a bathochromic shift.
RESUMEN
Black dye (BD), isomer 1 ([Ru(II)(H3-tctpy)(NCS)3](-1), where H3-tctpy = 4,4',4' '-tricarboxy-2,2':6,2' '-terpyridine) is known to be an excellent sensitizer for dye-sensitized solar cells and exhibits a very good near-IR photo response, compared to other ruthenium dyes. Because isothiocyanate is a linear ambidentate ligand, BD has three other linkage isomers, [Ru(H3-tctpy)(NCS)2(SCN)](-1), isomer 2 and 2', and [Ru(H3-tctpy))(SCN)3](-1), isomer 3. In this study, we have calculated the geometry of BD and its isomers by DFT. Further, we have analyzed the bonding in these isomers using NBO methods. TDDFT calculations combined with scalar relativistic zero-order regular approximations (SR-ZORA) have been carried out to simulate the absorption spectra. Calculations have been performed for the isomers both in vacuo and in solvent (ethanol). The inclusion of the solvent is found to be important to obtain spectra in good agreement with the experiment. The first absorption bands are dominated by the metal-to-ligand charge transfer (MLCT) and ligand-to-ligand charge transfer (LLCT).
RESUMEN
It is well-known from experimental studies that the oxyallyl-substructure-based squarylium and croconium dyes absorb in the NIR region of the spectrum. Recently, another dye has been reported (J. Am. Chem. Soc. 2003, 125, 348) which contains the same basic chromophore, but the absorption is red-shifted by at least 300 nm compared to the former dyes and is observed near 1100 nm. To analyze the reasons behind the large red shift, in this work we have carried out symmetry-adapted cluster-configuration interaction (SAC-CI) studies on some of these NIR dyes which contain the oxyallyl substructure. From this study, contrary to the earlier reports, it is seen that the donor groups do not seem to play a major role in the red-shift of the absorption. On the other hand, on the basis of the results of the high-level calculations carried out here and using qualitative molecular orbital theory, it is observed that the orbital interactions play a key role in the red shift. Finally, design principles for the oxyallyl-substructure-based NIR dyes are suggested.