RESUMEN
Despite continuous efforts, eutrophication is still occurring in freshwater and phosphorus (P) is the most important nutrients that drive the eutrophication in rivers and streams. However, little information is available about the distribution of P fractions in river sediment. Here, the sequential extraction approach was used to evaluate the sediment P fractionation and its content in the anthropogenically damaged river Ganga, India. Different sedimentary P fractions viz. exchangeable (Ex-P), aluminum bound (Al-P), iron bound (Fe-P), calcium bound (Ca-P), and organically bound phosphorus (Org-P), were quantified. Significantly higher level of total P was recorded in pre-monsoon season (438.5 ± 95.8 mg/kg), than other [winter (345.7 ± 110.6 mg/kg), post-monsoon (319.2 ± 136.3 mg/kg), and monsoon (288.6 ± 77.3 mg/kg)] seasons. Different P fractions such as Ex-P, Al-P, Fe-P, Ca-P and Org-P varied from 2.88-12.8 mg/kg, 7.64-98.8 mg/kg, 32.2-179.2 mg/kg, 51.97-286.1 mg/kg and 9.3-143.7 mg/kg, respectively, which correspondingly represented 0.5-10.54%, 3.41-20.18%, 17.27-37.82%, 37.35-60.2%, 4.15-25.88% of the Total P with a rank order of P-fractions was Ca-P > Fe-P > Org-P > Al-P > Ex-P. Bio-available P contributes a considerable portion (37.9-46.0%) of total P which may increase the eutrophication to overlying water. Results demonstrate that inorganic P species control the P bio-availability in both time and space. However, an estimated phosphorus pollution index based on sediment total P content showed no ecological risk of phosphorus to Ganga River sediment.
Asunto(s)
Monitoreo del Ambiente , Sedimentos Geológicos , Fósforo , Ríos , Estaciones del Año , Contaminantes Químicos del Agua , India , Fósforo/análisis , Ríos/química , Sedimentos Geológicos/química , Contaminantes Químicos del Agua/análisis , Medición de Riesgo , Monitoreo del Ambiente/métodos , Fraccionamiento Químico , EutrofizaciónRESUMEN
Consumption of toxic metal contaminated fish is a significant risk to human health. The Ganga river is one of the vital river systems in India, and it nurtures a rich biodiversity of flora and fauna. In the present study, screening of potential toxic metal elements (Cd, Cr, Mn, Cu, Pb, Ni, Zn, and As) was undertaken in 31 food fishes, especially the small indigenous fishes (SIFs) from the lower stretch of river Ganga by inductively coupled plasma mass spectrometry (ICP-MS). The concentration of toxic metal elements varied among different fish species. Among the toxic metal elements studied, Cr, Zn, and As were found to be dominant in Eleotris fusca; Cd, Ni, and Pb were highest in Securicula gora; Cu was highest in Cabdio morar; and Mn was highest in Coilia dussumieri. The average pollution load index values (APLI) for all the fishes analyzed were less than one except for Eleotris fusca, which indicated no serious toxic metal element pollution load. The estimated daily intake values (EDI) for the toxic metal elements were found to be within the permissible maximum tolerable daily intake (MTDI). Among the fishes studied, only a few species showed hazard index (HI) greater than one, indicating non-carcinogenic health risks. Similarly, the target carcinogenic values (TCR) for most of the toxic metal elements were below the permissible limit (10-4) in the fishes that assures minimal cancer risk. This study provides a comprehensive data on the composition of potential toxic metal elements of 31 food fishes from the lower stretch of the river Ganga, the first of its kind, and suggests the necessity of periodic monitoring of these in the aquatic ecosystem.
RESUMEN
A mutant, Δsll1252ins, was generated to functionally characterize Sll1252. Δsll1252ins exhibited a slow-growth phenotype at 70 µmol photons m-2 s-1 and glucose sensitivity. In Δsll1252ins, the rate of PSII activity was not affected, whereas the whole chain electron transport activity was reduced by 45%. The inactivation of sll1252 led to the upregulation of genes, which were earlier reported to be induced in DBMIB-treated wild-type, suggesting that Sll1252 may be involved in electron transfer from the reduced-PQ pool to Cyt b6/f. The inhibitory effect of DCMU on PSII activity was similar in both wild-type and Δsll1252ins. However, the concentration of DBMIB for 50% inhibition of whole chain electron transport activity was 140 nM for Δsll1252ins and 300 nM for wild-type, confirming the site of action of Sll1252. Moreover, the elevated level of the reduced-PQ pool in Δsll1252ins supports that Sll1252 functions between the PQ pool and Cyt b6/f. Interestingly, we noticed that Δsll1252ins reverted to wild-type phenotype by insertion of natural transposon, ISY523, at the disruption site. Δsll1252-Ntrn, expressing only the C-terminal region of Sll1252, exhibited a slow-growth phenotype and disorganized thylakoid structure compared to wild-type and Δsll1252-Ctrn (expressing only the N-terminal region). Collectively, our data suggest that Sll1252 regulates electron transfer between the PQ pool and the Cyt b6/f complex in the linear photosynthetic electron transport chain via coordinated function of both the N- and C-terminal regions of Sll1252.