Prediction of the molecular mechanism of eucommiae Cortex - Achyranthis bidentatae radix in the treatment of osteoarthritis: network pharmacology and molecular docking.

OBJECTIVE: To retrieve the core drug of osteoarthritis in clinic using Data Mining, predict the drug molecular action target through the Network Pharmacology, identify the key nodes of the interaction by combining with the related targtes of osteoarthritis, explore the pharmacological mechanism of Traditional Chinese Medicine against osteoarthritis and other possible mechanisms of actions. METHODS: to retrieve the commonly used therapeutic formulations for osteoarthritis patients in clinical with PubMed, CNKI, VIP, CBM, Wan Fang Database and other databases, and screen out the core drugs through the Ancient and Modern Medical Case Cloud Platform and software Gephi, filter out the core drug molecules and targets combined with TCMSP database and the targets of osteoarthritis in Genecard and OMIM database, plunge those data into R project and Cytoscape to construct the intersection model of Drug molecule-osteoarthritis, establish PPI network and GO and conduct KEGG enrichment analysis with String database. Vina molecular docking was finally implemented to draw molecular docking diagram, and the results were analyzed after comprehensive analysis. RESULTS: The core drug pairs were identified as 'Eucommiae Cortex - Achyranthis Bidentatae Radix' through correlation analysis, complex network analysis based on the coefficient. 'Eucommiae Cortex - Achyranthis Bidentatae Radix' can intervene cell behavior through multiple pathways and regulate cell metabolism, cytokine synthesis, oxidative and cellular immunity with the help of topology analysis in String Database. CONCLUSIONS: The core molecules of Quercetin and Kaempferol derived from 'Eucommia bark - achyranthes' can change the spatial conformation of PTGSs by hydrogen bonding with PTGSs, the hydrophobic bonds and van der Waals forces generated by Baicalein, Wogonin and ß-carotene, thereby changing the activity of PTGSs and affecting bone properties the process of osteoarthritis.

Chitosan-Based Surface Molecularly Imprinted Polymer Microspheres for Sustained Release of Sinomenine Hydrochloride in Aqueous Media.

The surface molecular imprinting technique has been proposed as a prospective strategy for template molecule recognition and separation by devising the recognition sites on the surface of imprinted materials. The purpose of this study was to establish a novel drug delivery system which was developed by surface molecular imprinting method using ß-cyclodextrin (ß-CD)-grafted chitosan (CS) (CS-g-ß-CD) microspheres as matrix and sinomenine hydrochloride (SM) as the template molecule. By adjusting the amount of functional monomer and cross-linking agent, we got the more excellent adsorption of CS-g-ß-CD molecularly imprinted polymers (MIPs-CS-g-ß-CD). When the amount of functional monomer was 6 mmol and cross-linking agent was 20 mmol, the maximum binding capacity of MIPs and non-imprinted polymers (NIPs) was 55.9 mg/g and 37.2 mg/g, respectively. The results indicated that the recognition of SM with MIPs was superior to NIPs. The adsorption isotherms of MIPs-CS-g-ß-CD indicated that the adsorption behavior fitted better to the Langmuir model, which showed that the adsorption process of polymer was monomolecular layer. In in vitro drug release studies, the accumulative release amount of MIPs-CS-g-ß-CD was up to 78% within 24 h. MIPs exhibited an excellent controlled SM release profile without burst release and the mechanism of SM release was shown to conform to non-Fick diffusion. Therefore, MIPs-CS-g-ß-CD were successfully applied to extraction of SM and used as the materials for drug delivery system.

[Clinical effects of total knee arthroplasty in the treatment of ankylosed knee].

OBJECTIVE: To investigate the clinical effects of total knee arthroplasty in treatment of ankylosed knee caused by various reasons. METHODS: Four patients with 7 ankylosed knees, caused by ankylosing spondylitis in 1 case, septic arthritis with bony ankylosis in 1 case, and rheumatoid arthritis in 2 cases, underwent artificial knee replacement. Before the operation joint activity was 0 degrees , Knee Society score (KSS) was 42 (11 - 63), and the function score was 17. Follow-up was conducted for 5 - 27 months. RESULTS: Follow-up showed that the average joint activity was raised to 83 degrees (60 degrees - 110 degrees ), KSS score to 83 (64 - 91) points, and function score to 77 points. No infectious case was found. CONCLUSION: Total knee arthroplasty has a satisfactory effect in treatment of ankylosed knee. Computer assisted navigation system is helpful in femoro-tibial osteotomy and soft tissue balance. Individualized and directed rehabilitation is a pivotal factor.

[Computer-assisted navigation for total knee arthroplasty: a comparative study with conventional methods].

OBJECTIVE: To compare the effects of Computer-assisted navigation (CAS) and conventional location technique in total knee arthroplasty (TKA). METHODS: Eight patients (11 knees) underwent CAS and 9 patients (11 knees) underwent conventional location technique for TKA. Follow-up was conducted for 5 - 12 months. The outcomes were compared. RESULTS: The mechanical axe error of the CAS group was (2.14 +/- 0.25) degrees, significantly less than that of the conventional group [(5.12 +/- 2.13) degrees, P = 0.005]. The soft tissue balance angle variable of the CAS group was (1.04 +/- 0.18) degrees, significantly less than that of the conventional group [(4.12 +/- 1.72) degrees, P = 0.005]. The soft tissue separation variable of the CAS group was (3.4 +/- 1.2) mm, significantly less than that of the conventional group [(6.1 +/- 2.4) mm, P = 0.005]. No fat embolism occurred in these 2 groups. The operating time of the CAS group was (82 +/- 18) min, significantly longer than that of the conventional group [(59 +/- 11) min, P = 0.005]. CONCLUSION: CAS causes more reliable artificial joint implantation and better soft-tissues balance, however, needs more operating time.