RESUMEN
Seven phenolic compounds (1-7) were isolated from the tubers of Gastrodia elata. Their structures were elucidated on the basis of MS and NMR spectral data. p-Ethoxymethyl phenyl-O-beta-D-glucoside (1) was proved to be a new compound, with N-(p-hydroxybenzyl)-adenosine (7) isolated from this plant for the first time. In this study, the protective effects of the six constituents (1-6) on PC12 cells against the cytotoxicity induced by KCl and glutamate were also investigated. The viability of the PC12 cells was significantly enhanced by pretreatment with the six phenolic constituents.
Asunto(s)
Adenosina/análogos & derivados , Antineoplásicos Fitogénicos/farmacología , Compuestos de Bencilo/farmacología , Éteres de Etila/farmacología , Gastrodia/química , Glucósidos/farmacología , Ácido Glutámico/toxicidad , Fenoles/farmacología , Cloruro de Potasio/antagonistas & inhibidores , Cloruro de Potasio/toxicidad , Adenosina/aislamiento & purificación , Adenosina/farmacología , Animales , Compuestos de Bencilo/aislamiento & purificación , Supervivencia Celular/efectos de los fármacos , Éteres de Etila/aislamiento & purificación , Antagonistas de Aminoácidos Excitadores/farmacología , Glucósidos/aislamiento & purificación , Espectroscopía de Resonancia Magnética , Espectrometría de Masas , Fármacos Neuroprotectores/aislamiento & purificación , Fármacos Neuroprotectores/farmacología , Células PC12 , Fenoles/aislamiento & purificación , Extractos Vegetales/química , Tubérculos de la Planta/química , Ratas , Sales de Tetrazolio , TiazolesRESUMEN
Two new ent-kaurane-type diterpenoids, E-semiaquilegin (1) and Z-semiaquilegin (2), together with eight known compounds (3-10) were isolated from the dried roots of Semiaquilegia adoxoides (DC.) Makino. The structures of compounds 1 and 2 were elucidated mainly by 2D NMR techniques including 1H-1H COSY, HSQC, HMBC, NOESY as 16alpha-hydroxy-ent-kaurane-17,20-di-(3,4-dihydroxy-E-cinnamoyl) ester and its (Z)-isomer.
Asunto(s)
Diterpenos/química , Diterpenos/aislamiento & purificación , Semiaquilegia/química , Estructura Molecular , Raíces de Plantas/químicaRESUMEN
A new flavonoid was isolated from the whole plant of Spiranthes australis (R. Brown) Lindl (Orchidacea). Its structure was characterized as 3,7-dimethoxy-5-hydroxy-2-([4-(3-methyl-2-butenyl)oxy]phenyl)-4H-1-benzopyran-4-one on the basis of chemical and spectral evidence including 2D NMR analysis.
