[Preparation and examination of the vitro releasing of pH-dependent kuijiekang tablet for colon-specific delivery].

OBJECTIVE: To prepare the pH-dependent kuijiekang tablet for colon-specific delivery used in treating the ulcerative colitis and to develop the in vitro release method of kuijiekang tablets. METHODS: The coating prescription was screened by the in vitro delivery of Puerarin and Berberine hydrochloride. The in-vitro releasing property of the preparation was examined by the method of in-vitro delivery. RESULTS: The preparation methods of the pH-dependent kuijiekang tablet for colon-specific delivery was obtained from the in-vitro delivery, Puerarin and Berberine hydrochloride were not detected in the simulated gastric fluid after 2 h and the quantitied of Puerarin and Berberine hydrochlo4de were less 10% in the simulated intestinal fluid after 4 h. The quantities of Puerarin and Berberine hydrochloride were 90.07% and 89.87%. CONCLUSION: It can be prepared and the preparation is a promising delivery system for drugs to be delivery to the colon.

[Determination of tanshinone II A and deoxyschizandrin in danshenwuweizi tablet by RP-HPLC].

OBJECTIVE: to set up a determination of tanshinone II A and deoxyschizaadrin in danshenwuweizi tablet by RP-HPLC. METHODS: two components were separated through Kromasil C18 (250 mm x 4.6 mm, 5 microm) column with methanol-water (85:15) by volume as a mobile phase. The flow rate was 1 ml x min(-1) and the detection wavelength were 270 nm (tanshinone II A) and 254 nm (deoxyschizandrin). RESULTS: The linear range of tanshinone II A and deoxyschizandrin were 0.3232 microg-1.616 microg and 0.525 microg-2.625 microg, respectively. The average recovery was 99.83% with RSD 0.36% for tanshinone II A and 100.2% with RSD 0.72% for deoxyschizandrin respectively. CONCLUSION: The method is simple, accurate and rapid with good reproducibility. It can be used for the quality control of danshenwuweizi tablet.