Inflammatory cytokines and two subtypes of breast cancer: A two-sample mendelian randomization study.

BACKGROUND: Breast cancer is a common cancer type that leads to cancer-related deaths among women. HER2-positive breast cancer, in particular, is associated with poor prognosis due to its high aggressiveness, increased risk of recurrence, and metastasis potential. Previous observational studies have explored potential associations between inflammatory cytokines and the risk of two breast cancer subtypes (HER2-positive and HER2-negative), but the results have been inconsistent. To further elucidate the causal relationship between inflammatory cytokines and the two breast cancer subtypes, we conducted a two-sample Mendelian randomization (MR) study. METHODS: We employed a two-sample bidirectional MR analysis using publicly available genome-wide association study (GWAS) statistics. After obtaining instrumental variables, we conducted MR analyses using five different methods to ensure the reliability of our results. Additionally, we performed tests for heterogeneity and horizontal pleiotropy. Subsequently, we conducted a reverse MR study by reversing exposure and outcome variables. RESULTS: Evidence from our IVW analysis revealed that genetically predicted levels of IL-5 [odds ratio (OR): 1.18, 95% confidence interval (CI): 1.04-1.35, P = 0.012], IL-7 (OR: 1.11, 95% CI: 1.01-1.22, P = 0.037), and IL-16 (OR: 1.13, 95% CI: 1.02-1.25, P = 0.025) were associated with an increased risk of HER2-positive breast cancer. Conversely, IL-10 (OR: 1.14, 95% CI: 1.03-1.26, P = 0.012) was associated with an increased risk of HER2-negative breast cancer. These results showed no evidence of heterogeneity or horizontal pleiotropy (P > 0.05). Results from the reverse MR analysis indicated no potential causal association between breast cancer and inflammatory cytokines (P > 0.05). CONCLUSION: Our findings demonstrate that IL-5, IL-7, and IL-16 are risk factors for HER2-positive breast cancer, with varying degrees of increased probability of HER2-positive breast cancer associated with elevated levels of these inflammatory cytokines. Conversely, IL-10 is a risk factor for HER2-negative breast cancer. Reverse studies have confirmed that breast cancer is not a risk factor for elevated levels of inflammatory cytokines. This series of results clarifies the causal relationship between different types of inflammatory cytokines and different subtypes of breast cancer. Based on this research, potential directions for the mechanism research of different inflammatory cytokines and different subtypes of breast cancer have been provided, and potential genetic basis for identifying and treating different subtypes of breast cancer have been suggested.

Telomere-to-telomere genome assembly of melon (Cucumis melo L. var. inodorus) provides a high-quality reference for meta-QTL analysis of important traits.

Melon is an important horticultural crop with extensive diversity in many horticultural groups. To explore its genomic diversity, it is necessary to assemble more high-quality complete genomes from different melon accessions. Meanwhile, a large number of QTLs have been mapped in several studies. Integration of the published QTLs onto a complete genome can provide more accurate information for candidate gene cloning. To address these problems, a telomere-to-telomere (T2T) genome of the elite melon landrace Kuizilikjiz (Cucumis melo L. var. inodorus) was de novo assembled and all the published QTLs were projected onto it in this study. The results showed that a high-quality Kuizilikjiz genome with the size of 379.2 Mb and N50 of 31.7 Mb was de novo assembled using the combination of short reads, PacBio high-fidelity long reads, Hi-C data, and a high-density genetic map. Each chromosome contained the centromere and telomeres at both ends. A large number of structural variations were observed between Kuizilikjiz and the other published genomes. A total of 1294 QTLs published in 67 studies were collected and projected onto the T2T genome. Several clustered, co-localized, and overlapped QTLs were determined. Furthermore, 20 stable meta-QTLs were identified, which significantly reduced the mapping intervals of the initial QTLs and greatly facilitated identification of the candidate genes. Collectively, the T2T genome assembly together with the numerous projected QTLs will not only broaden the high-quality genome resources but also provide valuable and abundant QTL information for cloning the genes controlling important traits in melon.

Study on Mechanical Behavior of Hollow-Core Slab Bridge with Pinned Reinforcement.

Joints connect prefabricated hollow-core slabs, the key elements of force transmission of hollow-core slab bridges. The joints are easily damaged, which affects the integrity and safety of the hollow-core slab bridge. This paper uses MIDAS FEA NX finite element analysis software to simulate the deep hinge joint segment model, comparing and analyzing the finite element simulation analysis results with the test results, and proposes the critical parameters of the hinge joint interface and concrete damage plasticity. Further, an assembled hollow slab bridge model is established to compare and analyze the force transfer performance of conventional and pinned reinforcement bridges and reveal the deep joint damage evolution process. The results showed that the hollow slab bridge damage appeared first at the hinge joint interface at the load location. Cracks in the joints can develop along the longitudinal and height directions, with the longitudinal crack length reaching 40% of the span. The vertical crack height can get the lower edge of the paving layer, increasing the distance from the load position, and the cracking height decreases symmetrically. Under an ultimate load, the hinge concrete of conventional reinforcement and pinned reinforcement hollow-core slab bridges showed significant damage in 30-70% and 40-60% of the span, respectively. Compared with the conventional reinforcement bridges, the cracking load and ultimate load of the pinned reinforcement bridges increase by 28.57% and 58.14%, respectively, and the relative deflection under 420 kN load reduces by 97.95%. The hollow slab bridges have improved the force performance and thus enhanced the integrity of the hollow-core slab bridges.

Study on Load Transfer Mechanism of Local Curved Prestressed Hollow-Core Slab Bridge.

The assembled hollow-core slab bridge is the most widely used beam bridge in China. With the increasing traffic volume and traffic load in China, the joints of the hollow-core slab bridge are prone to damage. In this paper, a hollow-core slab bridge with locally curved prestressed tendons is proposed. Based on the static load test of a beam with joints taken from the cross section of a hollow-core slab bridge in practical engineering, a finite element nonlinear analysis is used to simulate the test, and the concrete and interface parameters under the correct analysis results are obtained. Finally, the parameters are applied to the three-beam and two-joint hollow-core slab bridge with a span of 10 m and a finite element analysis is carried out to explore the total failure process and performance improvement effect of the prestressed hollow-core slab bridge. The results show that the interface unit method can successfully simulate the new-to-old concrete interface where the joint is in contact with the precast beam segment. Compared with the static load test results, the analysis error of each finite element model is basically within 15%. Compared with the traditional hollow-core slab bridge, the cracking load, through-joint load, and ultimate load of the prestressed hollow-core slab bridge are increased by 50.0%, 91.7%, and 66.7%, respectively. Under the same load, the stress of the U-bar, the relative deflection of both sides of the joint, and the maximum width of the joint of the prestressed hollow-core slab bridge are lower than those of the traditional hollow-core slab bridge. When the ultimate load is reached, the longitudinal crack lengths of the traditional hollow-core slab bridge and the prestressed hollow-core slab bridge are 0.48 L and 0.4 L, respectively, and the damage degree of the prestressed hollow-core slab bridge is lower than that of the traditional hollow-core slab bridge.

Impacts of stand density on diversity of understory plant and soil seed banks in a Pinus massoniana plantation.

Stand density is a critical factor impacting the diversity of understory plants. We analyzed the diversity of understory plants and soil seed banks, as well as their relationship by setting up three planting densities in a Pinus massoniana plantation, including low density (1575 trees·hm-2, D1), medium (2474 trees·hm-2, D2), and high (3550 trees·hm-2, D3). It aimed to provide a scientific basis for the implementation of the multi-objective sustainable development of plantations. The results showed that there were 70 species of herbs and shrubs belonging to 42 families and 62 genera. D1 was dominated by heliophiles, whereas both the D2 and D3 were dominated by shade-tolerant species. The Margalef (M), Shannon (H), Simpson (D), Pielou (Jsw), and Altalo (Al) indices of the herbs and shrubs exhibited a downward trend with increasing stand den-sity. In the herb layer, D1 and D3 showed significant difference in H, D, Jsw and Al. There were significant differences of Jsw and Al in the shrub layer among the three stand densities, but no diffe-rence of H and D. H, D, Jsw and Al in the soil seed bank first decreased and then increased with increasing stand density, with species richness and diversity being the highest in D1. The similarity coefficient of Jaccard and Sorensen among different stand densities was low. In the herb layer, M was positively correlated with Jsw. The correlations between stand density and H, D, Jsw and Al were greater in the shrub layer than in the herb layer. There was significant negative correlation between stand density and Jsw both in the shrub and herb layers. The stand density of 1575 trees·hm-2 was comparatively beneficial for the development of understory, plant diversity, and sustainability of P. massoniana plantation.

Mitochondrial ATP synthase regulates corpus cavernosum smooth muscle cell function in vivo and in vitro.

Adenosine triphosphate (ATP) levels are closely associated with diabetes-related erectile dysfunction (DMED). Mitochondrial ATP synthase serves a key role in ATP production. The present study aimed to investigate the relationship between F1-ATP synthase and DMED in vivo and in vitro. The F1-ATP synthase expression levels in corpus cavernosum tissues from rats with DMED were examined. F1-ATP synthase expression was found to be lower in corpus cavernosum tissues from rats with DMED compared with healthy controls, suggesting a role for ATP synthase under high glucose conditions. In addition, the present study also demonstrated that hyperglycemia could downregulate F1-ATP synthase expression in rat corpus cavernosum smooth muscle cells (CCSMCs) in vitro. The overexpression of F1-ATP synthase in CCSMCs influenced the phenotypic CCSMC transformation, upregulated eNOS expression, increased cGMP levels and reduced CCSMC apoptosis under high glucose in vitro. In conclusion, the present study indicates that the upregulation of mitochondrial ATP synthase expression may improve CCSMC function, suggesting that mitochondrial ATP synthase could serve as a potential therapeutic target for the treatment of DMED.

Comparative analysis of volatile compounds in thirty nine melon cultivars by headspace solid-phase microextraction and gas chromatography-mass spectrometry.

The types and amounts of volatiles in the fruits of 39 melon cultivars were determined. We identified 146 volatiles, including 55 esters, 23 aldehydes, 30 alcohols, 15 ketones, 6 acids and 17 others. Ethyl acetate, (Z)-6-nonenal and 3,6-(E,Z)-nonadien-1-ol were the most three abundant volatiles (average content > 50 µg/kg FW). Aroma profiles showed significant differences among cultivars. Zhongtian49 and Zhongtian20 had the most abundant aroma components (76) and Jinguniang exhibited the least (23). One non-climacteric inodorus cultivar (Xizhoumi25) had the highest content of total volatiles (1840 µg/kg FW). Principal component analysis clustered the 39 melon cultivars into five groups. This work describes the comparative diversity of melon fruit volatiles for a large number of cultivars. Furthermore, this study could support the selection of cultivars with a flavor that suits the public and also future breeding work towards the genetic improvement of melon flavor.

Study on the Preparation of Plasma-Modified Fly Ash Catalyst and Its De⁻NOX Mechanism.

Fly ash and bentonite were mixed in a certain proportion as raw materials to prepare a denitration catalyst. In previous studies, it has been concluded that fly ash-type catalysts can provide significant catalytic activity for denitrification after being modified with oxygen. In this study, the effect of plasma conditions on the denitration performance of the catalyst was investigated from the aspects of plasma modification power, modification time, and the flow rate of the gas. Boehm titration and infrared analysis systems were used to characterize the performance of the catalyst. The experimental results show that the optimal modification power is 60 W, the optimal modification time is 20 min, and the optimal gas flow rate is 40 mL/min.

Study on the NO removal efficiency of the lignite pyrolysis coke catalyst by selective catalytic oxidation method.

Selective catalytic oxidation (SCO) method is commonly used in wet denitration technology; NO after the catalytic oxidation can be removed with SO2 together by wet method. Among the SCO denitration catalysts, pyrolysis coke is favored by the advantages of low cost and high catalytic activity. In this paper, SCO method combined with pyrolysis coke catalyst was used to remove NO from flue gas. The effects of different SCO operating conditions and different pyrolysis coke catalyst made under different process conditions were studied. Besides, the specific surface area of the catalyst and functional groups were analyzed with surface area analyzer and Beohm titration. The results are: (1) The optimum operating conditions of SCO is as follows: the reaction temperature is 150°C and the oxygen content is 6%. (2) The optimum pyrolysis coke catalyst preparation processes are as follows: the pyrolysis final temperature is 750°C, and the heating rate is 44°C / min. (3) The characterization analysis can be obtained: In the denitration reaction, the basic functional groups and the phenolic hydroxyl groups of the catalyst play a major role while the specific surface area not.

Post-Operative Complications in Living Liver Donors: A Single-Center Experience in China.

The gap between the growing demand for available organs and the cadaveric organs facilitates the adoption of living donor liver transplantation. We retrospectively identified and evaluated the post-operative complications as per the modified Clavien classification system in 152 living liver donors at at the First Affiliated Hospital, College of Medicine, Zhejiang University between December, 2006 and June, 2014. Post-operative complications were observed in 61 patients (40.1%) in the present study, but no mortality was reported. Complications developed in 58 (40.0%) right, 1 (33.3%) left, and 2 (66.7%) lateral left hepatectomy donors. The prevalence of re-operation was 1.3%. Grade I and II complications were observed in 38 (25.0%) and 11 (7.2%) donors, respectively. Grade IIIa complications developed in 9 (5.9%) donors and only 3 (2.0%) patients reported grade IIIb complications. The most common complication was pleural effusion that occurred in 31 (20.4%) donors. No significant prognostic baseline factor was identified in this study. In conclusion, living donors experienced various complications, which were usually mild and had a good prognosis.