The mitochondrial genome of Bottapotamon fukienense (Brachiura: Potamidae) is fragmented into two chromosomes.

BACKGROUND: China is the hotspot of global freshwater crab diversity, but their wild populations are facing severe pressures associated with anthropogenic factors, necessitating the need to map their taxonomic and genetic diversity and design conservation policies. RESULTS: Herein, we sequenced the mitochondrial genome of a Chinese freshwater crab species Bottapotamon fukienense, and found that it is fragmented into two chromosomes. We confirmed that fragmentation was not limited to a single specimen or population. Chromosome 1 comprised 15,111 base pairs (bp) and there were 26 genes and one pseudogene (pseudo-nad1) encoded on it. Chromosome 2 comprised 8,173 bp and there were 12 genes and two pseudogenes (pseudo-trnL2 and pseudo-rrnL) encoded on it. Combined, they comprise the largest mitogenome (23,284 bp) among the Potamidae. Bottapotamon was the only genus in the Potamidae dataset exhibiting rearrangements of protein-coding genes. Bottapotamon fukienense exhibited average rates of sequence evolution in the dataset and did not differ in selection pressures from the remaining Potamidae. CONCLUSIONS: This is the first experimentally confirmed fragmentation of a mitogenome in crustaceans. While the mitogenome of B. fukienense exhibited multiple signs of elevated mitogenomic architecture evolution rates, including the exceptionally large size, duplicated genes, pseudogenisation, rearrangements of protein-coding genes, and fragmentation, there is no evidence that this is matched by elevated sequence evolutionary rates or changes in selection pressures.

Application of image recognition technology in pathological diagnosis of blood smears.

Traditional manual blood smear diagnosis methods are time-consuming and prone to errors, often relying heavily on the experience of clinical laboratory analysts for accuracy. As breakthroughs in key technologies such as neural networks and deep learning continue to drive digital transformation in the medical field, image recognition technology is increasingly being leveraged to enhance existing medical processes. In recent years, advancements in computer technology have led to improved efficiency in the identification of blood cells in blood smears through the use of image recognition technology. This paper provides a comprehensive summary of the methods and steps involved in utilizing image recognition algorithms for diagnosing diseases in blood smears, with a focus on malaria and leukemia. Furthermore, it offers a forward-looking research direction for the development of a comprehensive blood cell pathological detection system.

The application of functional imaging in visual field defects: a brief review.

Visual field defects (VFDs) represent a prevalent complication stemming from neurological and ophthalmic conditions. A range of factors, including tumors, brain surgery, glaucoma, and other disorders, can induce varying degrees of VFDs, significantly impacting patients' quality of life. Over recent decades, functional imaging has emerged as a pivotal field, employing imaging technology to illustrate functional changes within tissues and organs. As functional imaging continues to advance, its integration into various clinical aspects of VFDs has substantially enhanced the diagnostic, therapeutic, and management capabilities of healthcare professionals. Notably, prominent imaging techniques such as DTI, OCT, and MRI have garnered widespread adoption, yet they possess unique applications and considerations. This comprehensive review aims to meticulously examine the application and evolution of functional imaging in the context of VFDs. Our objective is to furnish neurologists and ophthalmologists with a systematic and comprehensive comprehension of this critical subject matter.

GDNet-EEG: An attention-aware deep neural network based on group depth-wise convolution for SSVEP stimulation frequency recognition.

Background: Steady state visually evoked potentials (SSVEPs) based early glaucoma diagnosis requires effective data processing (e.g., deep learning) to provide accurate stimulation frequency recognition. Thus, we propose a group depth-wise convolutional neural network (GDNet-EEG), a novel electroencephalography (EEG)-oriented deep learning model tailored to learn regional characteristics and network characteristics of EEG-based brain activity to perform SSVEPs-based stimulation frequency recognition. Method: Group depth-wise convolution is proposed to extract temporal and spectral features from the EEG signal of each brain region and represent regional characteristics as diverse as possible. Furthermore, EEG attention consisting of EEG channel-wise attention and specialized network-wise attention is designed to identify essential brain regions and form significant feature maps as specialized brain functional networks. Two publicly SSVEPs datasets (large-scale benchmark and BETA dataset) and their combined dataset are utilized to validate the classification performance of our model. Results: Based on the input sample with a signal length of 1 s, the GDNet-EEG model achieves the average classification accuracies of 84.11, 85.93, and 93.35% on the benchmark, BETA, and combination datasets, respectively. Compared with the average classification accuracies achieved by comparison baselines, the average classification accuracies of the GDNet-EEG trained on a combination dataset increased from 1.96 to 18.2%. Conclusion: Our approach can be potentially suitable for providing accurate SSVEP stimulation frequency recognition and being used in early glaucoma diagnosis.