miRNA-Based Early Healing Mechanism of Extraction Sockets: miR-190a-5p, a Potential Enhancer of Bone Healing.

Objective: Tooth extraction causes a wound with hard and soft tissue defects in the alveolar ridge. Few studies have reported the function of microRNAs (miRNAs) in the healing of extraction sockets. This study used bioinformatics analysis to reveal the possible relevance and role of miRNAs during the early stages following tooth extraction. Materials and Methods: Socket tissues from beagle dogs (Canis familiaris; two males and two females) were collected 1 and 12 hours after extraction of premolars on both sides of the mandible. miRNA expression was profiled through miRNA sequencing, and hub miRNAs showing characteristic expression patterns were selected and subjected to target enrichment analysis. Alkaline phosphatase (ALP) activity analysis and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) were performed to verify the effect of hub miRNA on osteoblast differentiation and bone regeneration in vivo. Results: Five miRNAs were identified to have consistently high expression levels, with cfa-miR-451 showing the highest expression. Additionally, 20 hub miRNAs were selected as candidates expected to play an important role in the healing process. Pathways, such as the MAPK, axon guidance, TGF-ß, and Wnt signaling, were significantly enriched. Among hub miRNAs, miR-190a-5p increased ALP activity and mRNA expression of osteogenic markers and increased new bone formation in vivo. Conclusions: Our findings suggest that miRNAs may be involved in the earliest stages of socket healing after tooth extraction and can play an important role in moderating the entire socket healing mechanism in the extraction socket.

Emission of Spin-Correlated Matter-Wave Jets from Spinor Bose-Einstein Condensates.

We report the observation of matter-wave jet emission in a strongly ferromagnetic spinor Bose-Einstein condensate of ^{7}Li atoms. Directional atomic beams with |F=1,m_{F}=1⟩ and |F=1,m_{F}=-1⟩ spin states are generated from |F=1,m_{F}=0⟩ state condensates or vice versa. This results from collective spin-mixing scattering events, where spontaneously produced pairs of atoms with opposite momentum facilitates additional spin-mixing collisions as they pass through the condensates. The matter-wave jets of different spin states (|F=1,m_{F}=±1⟩) can be a macroscopic Einstein-Podolsky-Rosen state with spacelike separation. Its spin-momentum correlations are studied by using the angular correlation function for each spin state. Rotating the spin axis, the inter- and intraspin-momentum correlation peaks display a high-contrast oscillation, indicating collective coherence of the atomic ensembles. We provide numerical calculations that describe the experimental results at a quantitative level. Our Letter paves the way to generating macroscopic quantum entanglement with the spin and motional degree of freedom with massive particles. It has a wide range of applications from quantum information science to the fundamental studies of quantum entanglement.

Hair Metabolomics in Animal Studies and Clinical Settings.

Metabolomics is a powerful tool used to understand comprehensive changes in the metabolic response and to study the phenotype of an organism by instrumental analysis. It most commonly involves mass spectrometry followed by data mining and metabolite assignment. For the last few decades, hair has been used as a valuable analytical sample to investigate retrospective xenobiotic exposure as it provides a wider window of detection than other biological samples such as saliva, plasma, and urine. Hair contains functional metabolomes such as amino acids and lipids. Moreover, segmental analysis of hair based on its growth rate can provide information on metabolic changes over time. Therefore, it has great potential as a metabolomics sample to monitor chronic diseases, including drug addiction or abnormal conditions. In the current review, the latest applications of hair metabolomics in animal studies and clinical settings are highlighted. For this purpose, we review and discuss the characteristics of hair as a metabolomics sample, the analytical techniques employed in hair metabolomics and the consequence of hair metabolome alterations in recent studies. Through this, the value of hair as an alternative biological sample in metabolomics is highlighted.

Ovarian Clear Cell Carcinoma Sub-Typing by ARID1A Expression.

PURPOSE: Loss of AT-rich DNA-interacting domain 1A (ARID1A) has been identified as a driving mutation of ovarian clear cell carcinoma (O-CCC), a triple-negative ovarian cancer that is intermediary between serous and endometrioid subtypes, in regards to molecular and clinical behaviors. However, about half of O-CCCs still express BAF250a, the protein encoded by ARID1A. Herein, we aimed to identify signatures of ARID1A-positive O-CCC in comparison with its ARID1A-negative counterpart. MATERIALS AND METHODS: Seventy cases of O-CCC were included in this study. Histologic grades and patterns of primary tumor, molecular marker immunohistochemistry profiles, and clinical outcomes were analyzed. RESULTS: Forty-eight (69%) O-CCCs did not express BAF250a, which were designated as "ARID1A-negative." The other 22 (31%) O-CCCs were designated as "ARID1A-positive." ARID1A-positive tumors were more likely to be histologically of high grades (41% vs. 10%, p=0.003), ERß-positive (45% vs. 17%, p=0.011), and less likely to be HNF1ß-positive (77% vs. 96%, p=0.016) and E-cadherin-positive (59% vs. 83%, p=0.028) than ARID1A-negative tumors. Patient age, parity, tumor stage were not significantly different in between the two groups. Cancer-specific survival was not significantly different either. CONCLUSION: We classified O-CCCs according to ARID1A expression status. ARID1A-positive O-CCCs exhibited distinct immunohistochemical features from ARID1A-negative tumors, suggesting a different underlying molecular event during carcinogenesis.

Exploring the many-body localization transition in two dimensions.

A fundamental assumption in statistical physics is that generic closed quantum many-body systems thermalize under their own dynamics. Recently, the emergence of many-body localized systems has questioned this concept and challenged our understanding of the connection between statistical physics and quantum mechanics. Here we report on the observation of a many-body localization transition between thermal and localized phases for bosons in a two-dimensional disordered optical lattice. With our single-site-resolved measurements, we track the relaxation dynamics of an initially prepared out-of-equilibrium density pattern and find strong evidence for a diverging length scale when approaching the localization transition. Our experiments represent a demonstration and in-depth characterization of many-body localization in a regime not accessible with state-of-the-art simulations on classical computers.

Observation of thermally activated vortex pairs in a quasi-2D Bose gas.

We measure the in-plane distribution of thermally activated vortices in a trapped quasi-2D Bose gas, where we enhance the visibility of density-depleted vortex cores by radially compressing the sample before releasing the trap. The pairing of vortices is revealed by the two-vortex spatial correlation function obtained from the vortex distribution. The vortex density decreases gradually as temperature is lowered, and below a certain temperature, a vortex-free region emerges in the center of the sample. This shows the crossover from a Berezinskii-Kosterlitz-Thouless phase containing vortex-pair excitations to a vortex-free Bose-Einstein condensate in a finite-size 2D system.

Observation of a geometric Hall effect in a spinor Bose-Einstein condensate with a Skyrmion spin texture.

For a spin-carrying particle moving in a spatially varying magnetic field, effective electromagnetic forces can arise due to the geometric phase associated with adiabatic spin rotation of the particle. We report the observation of a geometric Hall effect in a spinor Bose-Einstein condensate with a Skyrmion spin texture. Under translational oscillations of the spin texture, the condensate resonantly develops a circular motion in a harmonic trap, demonstrating the existence of an effective Lorentz force. When the condensate circulates, quantized vortices are nucleated in the boundary region of the condensate and the vortex number increases over 100 without significant heating. We attribute the vortex nucleation to the shearing effect of the effective Lorentz force from the inhomogeneous effective magnetic field.

Probing phase fluctuations in a 2D degenerate Bose gas by free expansion.

We measure the power spectrum of the density distribution of a freely expanding two-dimensional (2D) degenerate Bose gas, where irregular density modulations gradually develop due to initial phase fluctuations in the sample. The spectrum has an oscillatory shape, where the peak positions are found to be independent of temperature and show scaling behavior in the course of expansion. The relative intensity of phase fluctuations is estimated from the normalized spectral peak strength and observed to decrease at lower temperatures, confirming the thermal nature of the phase fluctuations. We investigate the relaxation dynamics of nonequilibrium states using the power spectrum. Free vortices are observed with ring-shaped density ripples in a perturbed sample after a long relaxation time.

Observation of topologically stable 2D Skyrmions in an antiferromagnetic spinor Bose-Einstein condensate.

We present the creation and time evolution of two-dimensional Skyrmion excitations in an antiferromagnetic spinor Bose-Einstein condensate. Using a spin rotation method, the Skyrmion spin textures were imprinted on a sodium condensate in a polar phase, where the two-dimensional Skyrmion is topologically protected. The Skyrmion was observed to be stable on a short time scale of a few tens of ms but to dynamically deform its shape and eventually decay to a uniform spin texture. The deformed spin textures reveal that the decay dynamics involves breaking the polar phase inside the condensate without having topological charge density flow through the boundary of the finite-sized sample. We discuss the possible formation of half-quantum vortices in the deformation process.

Development of coated nifedipine dry elixir as a long acting oral delivery with bioavailability enhancement.

To develop the long acting nifedipine oral delivery with bioavailability enhancement, a nifedipine dry elixir (NDE) containing nifedipine ethanol solution in dextrin shell was prepared using a spray-dryer, and then coated nifedipine dry elixir (CNDE) was prepared by coating NDE with Eudragit acrylic resin. The physical characteristics and bioavailability of NDE and CNDE were evaluated, and then compared to those of nifedipine powder. NDE and CNDE, which were spherical in shape, had about 6.64 and 8.68-8.75 µm of geometric mean diameters, respectively. The amount of nifedipine dissolved from NDE for 60 min increased about 7- and 40-fold compared to nifedipine powder in pH 1.2 simulated gastric fluid and pH 6.8 simulated intestinal fluid, respectively. Nifedipine released from CNDE was retarded in both dissolution media compared with that from NDE. After oral administration of NDE, the C(max) and AUC(0→8h) of nifedipine in rat increased about 13- and 7-fold, respectively, and the Tmax of nifedipine was reduced significantly compared with those after oral administration of nifedipine powder alone. The AUC(0→8h) and T(max) of nifedipine in CNDE increased markedly and the C(max) of nifedipine in CNDE was significantly reduced compared to those in NDE. It is concluded that CNDE, which could lower the initial burst-out plasma concentration and maintain the plasma level of nifedipine over a longer period with bioavailability enhancement, might be one of potential alternatives to the marketed long acting oral delivery system for nifedipine.

High efficient white organic light-emitting diodes using BCzVBi as blue fluorescent dopant.

Efficiency improvement and color optimization of white organic light-emitting diodes (WOLEDs) were achieved via employing blue host DPVBi doped with blue fluorescent, BCzVBi. The structure of high efficient WOLED device was composed of ITO/NPB/DPVBi:BCzVBi-6%/MADN:DCM2-0.5%/Bphen/Liq/Al. WOLED doped by blue fluorescent BCzVBi exhibits 6.19 cd/A of luminous efficiency and 15400 cd/m2 of maximum luminescence. It also performs 480 cd/m2 of luminance at 5.7 V and 15400 cd/m2 at 12.9 V with CIE(x,y) coordinates of (0.33, 0.32) and (0.32, 0.32), respectively. Hole carrier and energy transfer from DPVBi to BCzVBi are proposed to explain the observed phenomena.