An emergency situation for pen shells in the Mediterranean: The Adriatic Sea, one of the last Pinna nobilis shelters, is now affected by a mass mortality event.

We identified areas with high individual densities of the pen shell, Pinna nobilis, in two areas along the Croatian Adriatic coast. The surveys carried out in 2018 and 2019 showed population densities of approximately 9 to 13 individuals/100 m2. However, in 2019 a mass mortality event (MME) causing 36% to 100% mortality of this bivalve species was observed in the surveyed Croatian bays. The parasite Haplosporidium pinnae was identified by histological and molecular methods in all affected sampled individuals, while Mycobacterium sp. and Gram negative bacilli were detected in some affected and live bivalves. This finding constitutes the first record of these pathogens affecting P. nobilis in the middle Adriatic, confirming the continuous spread of the disease. Previously, the Adriatic water body was considered to be a natural shelter against the MME caused by pathogens in pen shell populations because of its distinct ecological features. The Adriatic Sea is a semi-closed water body with the largest continental shelf in the Mediterranean Sea, and due to its geomorphology and bathymetry, it is a sea with distinct characteristics. Monitoring plans and further studies in the Adriatic bays are now a priority for mitigating the high risk of extinction and working toward the conservation of this protected species.

Marine litter in sediments related to ecological features in impacted sites and marine protected areas (Croatia).

This study estimates levels of marine litter including plastics (macro-, meso- and microplastics) in sediments collected in shallow marine water from Croatia (Central Adriatic Sea). Selected sampling areas are characterized by different human pressure: Silba is highly stressed by tourism while Grebena is listed as marine protected area (MPA) of future institution. Obtained results on marine litter in sediments are correlated to ecological features recorded in each sampling site. Marine litter in collected sediments ranged within 180-528 items/kg d.w. Macroplastics were not recorded while mesoplastics were 1.3-4.8%. On a general basis, fibres are higher than microplastics. In Juzni Greben, level of fragments higher than fibres was recorded. A slight significance of the factor "water depth" was evidenced by the statistical analyses exploring relationships among marine litter and ecological features. Furthermore, Silba and Grebena Islands showed a significant different assessment of size, shape and colour features. These results suggest different sources/dynamics affecting marine litter recorded in marine areas stressed by tourism compared to MPA.

Plastic litter in sediments from the Croatian marine protected area of the natural park of Telascica bay (Adriatic Sea).

This paper reports baseline levels of litter (macro, meso and microplastics) in sediments collected from different areas of the Croatian MPA of the Natural Park of Telascica bay (Adriatic Sea, GSA n. 17). The distribution of total abundance according to size, for all analysed locations evidences that microplastics are the dominant fraction concerning item's numbers. In all analysed samples no macroplastics were found, while microplastics are 88.71% and mesoplastics are 11.29% of the total.

Plastic litter in sediments from a marine area likely to become protected (Aeolian Archipelago's islands, Tyrrhenian sea).

This research aims to define for the first time levels and patterns of different litter groups (macro, meso and microplastics) in sediments from a marine area designed for the institution of a new marine protected area (Aeolian Archipelago, Italy). Microplastics resulted the principal group and found in all samples analyzed, with shape and colours variable between different sampling sites. MPs levels measured in this study are similar to values recorded in harbour sites and lower than reported in Adriatic Sea, while macroplastics levels are notably lower than in harbor sites. Sediment grain-size and island extent resulted not significant in determining levels and distribution of plastic debris among islands. In the future, following the establishment of the MPA in the study area, these basic data will be useful to check for potential protective effects on the levels and distribution of plastic debris.