Glyphosate hormesis stimulates tomato (Solanum lycopersicum L.) plant growth and enhances tolerance against environmental abiotic stress by triggering nonphotochemical quenching.

BACKGROUND: Glyphosate is the most widely applied herbicide in the world. Hormesis caused by low glyphosate doses has been widely documented in many plant species. However, the specific adaptative mechanism of plants responding to glyphosate hormesis stimulation remains unclear. This study focused on the biphasic relationship between glyphosate dose and tomato plant growth, and how glyphosate hormesis stimulates plant growth and enhances tolerance to environmental stress. RESULTS: We constructed a hormesis model to describe the biphasic relationship with a maximal stimulation (MAX) of 162% above control by glyphosate at 0.063 g ha-1. Low-dose glyphosate increased photosynthetic pigment contents and improve photosynthetic efficiency, leading to plant growth stimulation. We also found that glyphosate hormesis enhanced plant tolerance to diuron (DCMU; a representative photosynthesis inhibitor) by triggering the nonphotochemical chlorophyll fluorescence quenching (NPQ) reaction to dissipate excess energy stress from photosystem II (PSII). Transcriptomic analysis and quantitative real-time polymerase chain reaction results revealed that the photosynthesis-antenna proteins pathway was the most sensitive to glyphosate hormesis, and PsbS (encoding photosystem II subunit S), ZEP (encoding zeaxanthin epoxidase) and VDE (encoding violaxanthin de-epoxidase) involved in NPQ played crucial roles in the plant response to glyphosate hormesis. CONCLUSION: These results provide novel insights into the mechanisms of plant hormesis and is meaningful to the application of glyphosate hormesis in agriculture. © 2024 Society of Chemical Industry.

Hormesis effects in tomato plant growth and photosynthesis due to acephate exposure based on physiology and transcriptomic analysis.

BACKGROUND: Hormesis is a common phenomenon in toxicology described as low-dose stimulation due to a toxin which causes inhibition at a high dose. Pesticide hormesis in plants has attracted considerable research interest in recent years; however, the specific mechanism has not yet been clarified. Acephate is an organophosphorus insecticide that is used worldwide. Here, hormesis in tomato (Solanum lycopersicum L.) plant growth and photosynthesis after acephate exposure is confirmed, as stimulation occurred at low stress levels, whereas inhibition occurred after exposure to high concentrations. RESULTS: We found that low acephate concentration (5-fold lower than recommended application dosage) could enhance chlorophyll biosynthesis and stimulate photosynthesis effects, and thus improve S. lycopersicum growth. A high level of acephate (5-fold higher than recommended application dosage) stress inhibited chlorophyll accumulation, decreased photosystem II efficiency and blocked antioxidant reactions in leaves, increasing reactive oxygen species levels and damaging plant growth. Transcriptomic analysis and quantitative real-time PCR results revealed that the photosynthesis - antenna proteins pathway played a crucial role in the hormesis effect, and that LHCB7 as well as LHCP from the pathway were the most sensitive to acephate hormesis. CONCLUSION: Our results showed that acephate could induce hormesis in tomato plant growth and photosynthesis, and that photosystem II and the photosynthesis - antenna proteins pathway played important roles in hormesis. These results provide novel insights into the scientific and safe application of chemical pesticides, and new guidance for investigation into utilizing pesticide hormesis in agriculture. © 2023 Society of Chemical Industry.

AMFormulaS: an intelligent retrieval system for traditional Chinese medicine formulas.

BACKGROUND: Formula is an important means of traditional Chinese medicine (TCM) to treat diseases and has great research significance. There are many formula databases, but accessing rich information efficiently is difficult due to the small-scale data and lack of intelligent search engine. METHODS: We selected 38,000 formulas from a semi-structured database, and then segmented text, extracted information, and standardized terms. After that, we constructed a structured formula database based on ontology and an intelligent retrieval engine by calculating the weight of decoction pieces of formulas. RESULTS: The intelligent retrieval system named AMFormulaS (means Ancient and Modern Formula system) was constructed based on the structured database, ontology, and intelligent retrieval engine, so the retrieval and statistical analysis of formulas and decoction pieces were realized. CONCLUSIONS: AMFormulaS is a large-scale intelligent retrieval system which includes a mass of formula data, efficient information extraction system and search engine. AMFormulaS could provide users with efficient retrieval and comprehensive data support. At the same time, the statistical analysis of the system can enlighten scientific research ideas and support patent review as well as new drug research and development.

Biological mechanisms of glycan- and glycosaminoglycan-based nutraceuticals.

A nutraceutical is defined as a standardized pharmaceutical-grade nutrient. Among hundreds of nutraceuticals, polysaccharide or glycan-based products such as those containing chondroitin sulfate glycosaminoglycan isolated from animal cartilage have been on the top nutraceutical selling list for many years. It is expected that the nutraceutical market will reach $250 billion dollars worldwide by 2018. Glycans are most abundant biopolymers on earth those are synthesized by bacteria, fungi, plants, insects, and animals. Glycans that are synthesized by animals or from all marine sources can be modified with covalently linked sulfates or containing acidic monosaccharides whereas glycans that are synthesized by terrestrial plants or fungi usually do not contain sulfates. Glycans such as starch are common sources of energy for animals, therefore they are on the nutrient-side of nutraceuticals. Undigestible polysaccharides from plants could serve as dietary fiber for humans that change the contents of the gastrointestinal tract and affect how other nutrients and chemicals are absorbed, thus dietary fibers could be called nutraceuticals. Other intra- and extracellular glycans from different sources serve as biological active components that regulate a myriad of physiological functions. The reported biological functions for such glycans are not limited to immune system regulatory, anti-coagulating, anti-tumor, anti-viral, anti-oxidant, anti-aging, and lipid-lowing activities, which make them pharmaceutical-side nutraceuticals. This review will present the full spectrum of glycan-based nutraceuticals and summarize current knowledge (published data from 1960s to current) of the structure, biological function, and mechanisms of glycans from both terrestrial and marine sources.

The sensitivity and specificity of serum glycan-based biomarkers for cancer detection.

Most of clinically used serum biomarkers for cancer detection were established in early 1980s when the Nobel Prize in physiology or medicine was awarded for the "discovery of the principle for the production of monoclonal antibodies." Using this "Nobel" technology, various monoclonal antibodies were obtained when different types of cancer cells were injected into mice and the ligands on the cancer cell surface were characterized. Both aberrant glycan structures and aberrant glycan-associated glycoproteins were revealed as a common feature of cancer cell surfaces through the specific interactions with the monoclonal antibodies. These results indicate that the biosynthesis of the environment-sensitive glycan structures goes awry in cancer cells, which is beyond genetic mutations. Later on, the glycan-related biomarkers were detected in the sera of cancer patients and then developed into serum biomarkers, such as CA125, CA153, CA195, CA199, CA242, CA27.29, CA50, and CA724, which are still in clinical use as of today. During the past 30 years, even with the advancement of different OMICS technologies not limited to genomics, epigenomics, proteomics, glycomics, lipidomics, and metabolomics, very few serum biomarkers have been introduced into clinical practice. The reason is that most of the newly discovered cancer biomarkers are inferior in terms of sensitivity and specificity to these biomarkers. We will summarize the reported sensitivity and specificity of currently used cancer biomarkers, especially the glycan-related biomarkers, in the forms of tables and radar plots and discuss the pros and cons of currently used cancer biomarkers.

Norcantharidin Inhibits cell growth by suppressing the expression and phosphorylation of both EGFR and c-Met in human colon cancer cells.

BACKGROUND: Norcantharidin (NCTD) is a Chinese FDA approved, chemically synthesized drug for cancer treatment. The effect of NCTD on signaling proteins of EGFR and c-Met was systematically elucidated in current study. METHODS: Two human colon cancer cell lines, HCT116 and HT29, were used as model systems to investigate the anti-cancer molecular mechanism of NCTD. Cell cycle arrest and early/late apoptosis were analyzed by flow cytometry. The levels of EGFR, phospho-EGFR, c-Met, phospho-c-Met and other related proteins were quantified by western blot analysis. RESULTS: NCTD induced cell cycle arrest at G2/M phase in both cell lines. The early and late apoptosis was also observed. Further investigation indicated that NCTD suppressed not only the expression of the total EGFR and the phosphorylated EGFR but also the expression of the total c-Met and the phosphorylated c-Met in colon cancer cells. Moreover, EGFR expression could be mostly restored by co-treatment with MG132, a proteasome inhibitor. In addition, NCTD-induced cell death was comparable to that of the anti-cancer drug gefitinib, a tyrosine kinase inhibitor for EGFR, based on the immunoblot analysis of the expressed proteins after the drug treatment. CONCLUSIONS: NCTD might be a useful and inexpensive drug candidate to substitute for gefitinib to serve the treatment needs of cancer patients.

Efficacy of Heparinoid PSS in Treating Cardiovascular Diseases and Beyond-A Review of 27 Years Clinical Experiences in China.

Propylene glycol alginate sodium sulfate (PSS) is the world's first oral heparinoid approved by Chinese Food and Drug Administration in 1987. Propylene glycol alginate sodium sulfate is produced by modifying partially hydrolyzed alginate, one of the most abundant marine polysaccharides isolated from brown algae, by epoxypropane esterification and by chemical sulfation. It is used for treating and preventing cardiovascular-related diseases. The low cost (US$1.29/100 tablets, ∼4 tablets/day), remarkable clinical effects, and convenient oral administration make PSS an ideal long-term prevention drug. Propylene glycol alginate sodium sulfate is available in most drug stores in China, and millions of patients take PSS routinely during the past 27 years. The 22 784 reported clinical cases as well as the structure, preparation, clinical efficacy, adverse reactions, pharmacokinetics, pharmacodynamics, and future perspectives of PSS based on the results of peer-reviewed publications will be discussed. This review should bring the knowledge of PSS gained in China to the world to stimulate in depth academic and clinical studies of PSS.

An efficient synthesis method targeted to marine alkaloids marinacarbolines A-D and their antitumor activities.

Marinacarbolines A-D are a series of marine ß-carboline alkaloids isolated from actinomycete Marinactinospora thermotolerans of the deep South China Sea with antiplasmodial activities. In inhibition assays of in vitro growth of Plasmodium falciparum, marinacarbolines exhibited antiplasmodial activity against drug-sensitive line 3D7 and drug-resistant line Dd2 of P. falciparum. However, approaches for the synthesis of such useful compounds are very limited. In this work, we reported a simple, efficient, and versatile process to synthesize marinacarbolines A-D (1-4). On the basis of that, the antitumor activities of marinacarbolines in a structure-dependent manner were allowed to be unveiled.