The Application Value of Clinical Intervention Approaches Based on the Guidance of Knowledge, Belief, and Action Theory for Children with AB and the Analysis of Risk Factors for Poor Prognosis.

In order to explore the clinical efficacy of knowledge, information, and action theory combined with clinical nursing in children with asthmatic bronchitis (AB) and to analyze the influencing factors of poor prognosis, a total of 98 children with AB in our hospital from January 2021 to August 2022 are collected. The baseline data are analyzed and are randomly divided into a combination group (n = 49) and a single group (n = 49). The experimental results show that the baseline data of the research subjects are not comparable (P > 0.05), the clinical efficacy of the combined group is higher than that of the single group, and the level of pulmonary function indexes in the combined group is significantly higher than that of the single group (P < 0.05). The observation shows that family history, repeated respiratory virus infection, and allergy history are all risk factors affecting the prognosis of children with AB.

Efficacy and safety of umbilical cord mesenchymal stem cells for the treatment of patients with COVID-19.

OBJECTIVES: The coronavirus disease (COVID-19) outbreak has catastrophically threatened public health worldwide and presented great challenges for clinicians. To date, no specific drugs are available against severe acute respiratory syndrome coronavirus 2. Mesenchymal stem cells (MSCs) appear to be a promising cell therapy owing to their potent modulatory effects on reducing and healing inflammation-induced lung and other tissue injuries. The present pilot study aimed to explore the therapeutic potential and safety of MSCs isolated from healthy cord tissues in the treatment of patients with COVID-19. METHODS: Twelve patients with COVID-19 treated with MSCs plus conventional therapy and 13 treated with conventional therapy alone (control) were included. The efficacy of MSC infusion was evaluated by changes in oxygenation index, clinical chemistry and hematology tests, immunoglobulin (Ig) levels, and pulmonary computerized tomography (CT) imaging. The safety of MSC infusion was evaluated based on the occurrence of allergic reactions and serious adverse events. RESULTS: The MSC-treated group demonstrated significantly improved oxygenation index. The area of pulmonary inflammation decreased significantly, and the CT number in the inflammatory area tended to be restored. Decreased IgM levels were also observed after MSC therapy. Laboratory biomarker levels at baseline and after therapy showed no significant changes in either the MSC-treated or control group. CONCLUSION: Intravenous infusion of MSCs in patients with COVID-19 was effective and well tolerated. Further studies involving a large cohort or randomized controlled trials are warranted.

Efficacy and safety of umbilical cord mesenchymal stem cells for the treatment of patients with COVID-19

OBJECTIVES: The coronavirus disease (COVID-19) outbreak has catastrophically threatened public health worldwide and presented great challenges for clinicians. To date, no specific drugs are available against severe acute respiratory syndrome coronavirus 2. Mesenchymal stem cells (MSCs) appear to be a promising cell therapy owing to their potent modulatory effects on reducing and healing inflammation-induced lung and other tissue injuries. The present pilot study aimed to explore the therapeutic potential and safety of MSCs isolated from healthy cord tissues in the treatment of patients with COVID-19. METHODS: Twelve patients with COVID-19 treated with MSCs plus conventional therapy and 13 treated with conventional therapy alone (control) were included. The efficacy of MSC infusion was evaluated by changes in oxygenation index, clinical chemistry and hematology tests, immunoglobulin (Ig) levels, and pulmonary computerized tomography (CT) imaging. The safety of MSC infusion was evaluated based on the occurrence of allergic reactions and serious adverse events. RESULTS: The MSC-treated group demonstrated significantly improved oxygenation index. The area of pulmonary inflammation decreased significantly, and the CT number in the inflammatory area tended to be restored. Decreased IgM levels were also observed after MSC therapy. Laboratory biomarker levels at baseline and after therapy showed no significant changes in either the MSC-treated or control group. CONCLUSION: Intravenous infusion of MSCs in patients with COVID-19 was effective and well tolerated. Further studies involving a large cohort or randomized controlled trials are warranted.

Adenovirus encoding XAF-1 and TNF­α in the same open reading frame efficiently inhibits hepatocellular cancer cells.

X­linked inhibitor of apoptosis (XIAP)­associated factor 1 (XAF­1), a tumor suppressor, is downregulated in most human malignant tumors. However, the tumor suppressive role of XAF­1 in hepatocellular carcinoma (HCC) and its therapeutic value require further elucidation. The present study examined the expression of XAF­1 at the mRNA and protein level in the HCC and paired peritumor tissue specimens, as well as in HCC cell lines and a normal liver cell line. A recombinant adenovirus which co­expressed XAF­1 and TNF­α was then constructed, and its effects on the proliferation and colony formation ability of the MHCC97H HCC cell line were assessed using apoptosis induction, flow cytometry, trypan blue staining assay and a clonogenic assay. The results demonstrated that the expression of XAF­1 was significantly reduced in HCC tissues compared with that in their matched peritumor specimens, and a significant correlation with the tumor size, stage and tumor ­ nodes ­ metastasis stage was identified. The reduced levels of XAF­1 were further confirmed the HCC cell lines MHCC97L, HepG2 and MHCC97H compared with those in the L02 normal liver cell line. The recombinant adenovirus Ad­XAF­1&TNF­α, which co­expressed XAF­1 and TNF­α, was shown to efficiently express the two proteins at the mRNA and protein level. Furthermore, infection with Ad­XAF­1&TNF­α synergistically induced apoptosis, reduced the proliferation and colony formation ability of MHCC97L cells to a significantly greater extent than overexpression of XAF­1 or TNF­α individually. To the best of our knowledge, the present study was the first to construct an adenovirus which co­expressed XAF­1 and TNF­α in the same open reading frame and expressed them proportionally. As Ad­XAF­1&TNF­α inhibited HCC cells with enhanced efficiency, it may be applicable for the treatment of HCC.