RESUMEN
It is acknowledged that neurotransmission in the mouse vas deferens is predominantly mediated by ATP and noradrenaline (NA) released from sympathetic nerves while cholinergic transmission in the rodent vas deferens is often overlooked despite early literature. Recently we have characterized a cholinergic component of neurogenic contraction of mouse isolated vas deferens. In the present paper, by confocal imaging of Ca(2+) dynamics we detected acetylcholine (ACh) action at muscarinic cholinergic neuroeffector junctions at high-resolution. Experiments were carried out in the presence of prazosin (100 nM) and alpha,beta methylene ATP (alpha,beta-MeATP) (1 microM) to inhibit responses to NA and ATP respectively. Exogenous ACh (10 microM) elicited Ca(2+) transients, an effect blocked by the muscarinic receptor antagonist, cyclopentolate (1 microM). Ca(2+) transients were evoked by electrical stimulation of intrinsic nerves in the presence of the cholinesterase inhibitor neostigmine (10 microM). Stimulation produced a marked increase in the frequency and number of Ca(2+) transients. Cyclopentolate reduced the frequency of occurrence of spontaneous and evoked events to control levels. The alpha(2)-adrenoceptor antagonist yohimbine (300 nM) did not affect the spontaneous Ca(2+) transients, but increased the frequency of occurrence of evoked transients, an effect inhibited by cyclopentolate. The postjunctional effects of neuronally-released ACh are limited by the action of cholinesterase. Release of ACh appears to be tonically inhibited by NA released from sympathetic nerve terminals through action at prejunctional alpha(2)-adrenoceptors. Tetrodotoxin (TTX, 300 nM) abolished the nerve-evoked Ca(2+) events, with no effect on Ca(2+) transients elicited by exogenous ACh. In conclusion, the presence of spontaneous and evoked cholinergic Ca(2+) transients in smooth muscle cells of the mouse isolated vas deferens has been revealed. These events are mediated by ACh acting at M(3) muscarinic receptors. This action stands in marked contrast to the lack of effect of neuronally-released NA on smooth muscle Ca(2+) dynamics in this tissue.
Asunto(s)
Unión Neuroefectora/metabolismo , Receptor Muscarínico M3/fisiología , Conducto Deferente/fisiología , Acetilcolina/metabolismo , Acetilcolina/farmacología , Antagonistas de Receptores Adrenérgicos alfa 2 , Factores de Edad , Animales , Calcio/fisiología , Inhibidores de la Colinesterasa/farmacología , Ciclopentolato/farmacología , Técnicas In Vitro , Masculino , Ratones , Ratones Endogámicos BALB C , Microscopía Confocal , Contracción Muscular , Músculo Liso/inervación , Músculo Liso/fisiología , Neostigmina/farmacología , Neuronas/metabolismo , Norepinefrina/metabolismo , Piperidinas/farmacología , Receptor Muscarínico M3/antagonistas & inhibidores , Receptores Adrenérgicos alfa 2/fisiología , Conducto Deferente/inervación , Yohimbina/farmacologíaRESUMEN
BACKGROUND AND PURPOSE: The antidysrhythmic bretylium is useful experimentally because it selectively abolishes neurotransmitter release from sympathetic peripheral nerve terminals. Its mechanism of action seemed settled, but recent results from optical monitoring of single terminals now suggests a new interpretation. EXPERIMENTAL APPROACH: Orthograde transport of a dextran-conjugated Ca(2+) indicator to monitor Ca(2+) in nerve terminals of mouse isolated vas deferens with a confocal microscope. In some experiments, local neurotransmitter release was detected by monitoring neuroeffector Ca(2+) transients (NCTs) in adjacent smooth muscles, a local measure of purinergic transmission. Sympathetic terminals were identified with catecholamine fluorescence (UV excitation) or post-experiment immunohistochemistry. KEY RESULTS: Bretylium (10 microM) abolished NCTs at 60/61 junctions over the course of 2 h, indicating effective abolition of neurotransmitter release. However, bretylium did not abolish the field stimulus-induced Ca(2+) transient in most nerve terminals, but did increase both action potential delay (by 2+/-0.4 ms) and absolute refractory period (by 4+/-2 ms). Immunohistochemistry demonstrated that 85-96% of terminals orthogradely filled with a dextran-conjugated fluorescent probe contained Neuropeptide Y (NPY). A formaldehyde-glutaraldehyde-induced catecholamine fluorescence (FAGLU) technique was modified to allow sympathetic terminals to be identified with a Ca(2+) indicator present. Most terminals contained catecholamines (based on FAGLU) or secrete ATP (as NCTs in adjacent smooth muscle cells are abolished). CONCLUSIONS AND IMPLICATIONS: Bretylium can inhibit neurotransmitter release downstream of Ca(2+) influx without abolishing the nerve terminal action potential. Bretylium-induced increases in the absolute refractory period permit living sympathetic terminals to be identified.
Asunto(s)
Potenciales de Acción/efectos de los fármacos , Compuestos de Bretilio/farmacología , Neurotransmisores/metabolismo , Terminales Presinápticos/efectos de los fármacos , Sistema Nervioso Simpático/efectos de los fármacos , Transmisión Sináptica/efectos de los fármacos , Conducto Deferente/inervación , Adenosina Trifosfato/metabolismo , Animales , Señalización del Calcio/efectos de los fármacos , Catecolaminas/metabolismo , Inmunohistoquímica/métodos , Masculino , Ratones , Ratones Endogámicos BALB C , Microscopía Confocal/métodos , Neuropéptido Y/metabolismo , Terminales Presinápticos/metabolismo , Sistema Nervioso Simpático/metabolismo , Factores de TiempoRESUMEN
Simultaneous electrophysiology and confocal microscopy were used to investigate purinergic neurotransmission at single smooth muscle cells (SMCs) in mouse isolated vas deferens, and to explore the relationship between two high-resolution P2X-receptor-mediated measures of per pulse ATP release: transient peaks in the first time derivative of the rising phase of excitatory junction potentials (EJPs) recorded in single SMCs ('discrete events'; DEs) and neuroeffector Ca(2+) transients (NCTs) in the impaled SMCs. This study shows that discrete events represent neurotransmitter release onto the impaled cell. First, the median amplitude of the first derivative of the EJP was larger when there was a coincident NCT in the impaled cell, compared with instances when no coincident NCT occurred. Second, the time-to-peak amplitude of the first derivative was shorter if there was a coincident NCT in the impaled cell, compared with when no coincident NCT was observed within the field. Surprisingly, first derivative amplitude increased with the distance (of the corresponding NCT) from the microelectrode. The microelectrode did not locally inhibit the functional quantal size as there was no effect of distance on the normalized NCT amplitude. When the significant effect of distance (between the microelectrode and NCTs) on the first derivative amplitude was removed, there was no correlation between the unstandardized residual (of distance vs. first derivative amplitude) and NCT amplitude. The absence of a correlation between DE and NCT amplitudes suggests that the NCT amplitude is a poor measure of quantal size. The usefulness of NCTs hence lies primarily in locating neurotransmitter release and measuring changes in local release probability.
Asunto(s)
Adenosina Trifosfato/metabolismo , Miocitos del Músculo Liso/metabolismo , Receptores Purinérgicos P2/metabolismo , Fibras Simpáticas Posganglionares/metabolismo , Transmisión Sináptica/fisiología , Conducto Deferente/metabolismo , Adenosina Trifosfato/farmacología , Animales , Señalización del Calcio/efectos de los fármacos , Señalización del Calcio/fisiología , Electrofisiología/métodos , Potenciales Postsinápticos Excitadores/efectos de los fármacos , Potenciales Postsinápticos Excitadores/fisiología , Masculino , Ratones , Ratones Endogámicos BALB C , Microscopía Confocal , Miocitos del Músculo Liso/efectos de los fármacos , Neurotransmisores/metabolismo , Técnicas de Cultivo de Órganos , Terminales Presinápticos/metabolismo , Receptores Purinérgicos P2X , Fibras Simpáticas Posganglionares/efectos de los fármacos , Transmisión Sináptica/efectos de los fármacos , Vesículas Sinápticas/metabolismo , Factores de Tiempo , Conducto Deferente/inervaciónRESUMEN
BACKGROUND AND PURPOSE: Nicotinic agonists increase sympathetic field-stimulus-evoked contraction of the rodent vas deferens, presumably by increasing evoked neurotransmitter release. This presumption was tested in two species. EXPERIMENTAL APPROACH: The effect of the nicotinic acetylcholine receptor (nAChR) agonist epibatidine on neurotransmitter release in mouse and guinea pig isolated vas deferens was investigated using contraction studies and conventional intracellular recording techniques. KEY RESULTS: In 12 of 14 mouse vasa deferentia, slow bath application of epibatidine (100 nM) had no significant effect on excitatory junction potential (EJP) amplitude and spontaneous EJP (SEJP) frequency. However, rapid application of epibatidine to the mouse vas deferens caused an increase in SEJP frequency (by 530%), with no effect on EJP amplitude. Despite the absence of an effect on EJPs, electrically-evoked contractions of the mouse vas deferens were significantly increased in the presence of epibatidine (by 50%). A transient contraction was reliably induced by a higher epibatidine concentration (1 microM). This contraction was significantly reduced in the presence of prazosin, tetrodotoxin, or alpha,beta-methyleneATP. Epibatidine did not induce a contraction in the presence of a combination of prazosin, alpha,beta-methyleneATP and cyclopentolate. In guinea pig vasa deferentia, bath-applied epibatidine potentiated EJP amplitude in a biphasic pattern, lasting for at least 30 minutes. CONCLUSION AND IMPLICATIONS: The nAChR-mediated augmentation of neurogenic contraction is indeed prejunctional, but in the mouse arises from an increase in spontaneous neurotransmitter release that primes smooth muscle for subsequent contraction, while in the guinea pig there is a direct augmentation of evoked neurotransmitter (ATP) release.
Asunto(s)
Compuestos Bicíclicos Heterocíclicos con Puentes/farmacología , Agonistas Nicotínicos/farmacología , Piridinas/farmacología , Receptores Nicotínicos/fisiología , Conducto Deferente/efectos de los fármacos , Animales , Cobayas , Técnicas In Vitro , Contracción Isométrica/efectos de los fármacos , Masculino , Potenciales de la Membrana/efectos de los fármacos , Ratones , Ratones Endogámicos BALB C , Conducto Deferente/fisiologíaRESUMEN
The skewed amplitude distribution of spontaneous excitatory junction potentials (sEJPs) in the mouse vas deferens and other electrically-coupled smooth muscle syncytia has been attributed to electrically-attenuated depolarizations resulting from the spontaneous release of quantized packets of ATP acting on remote smooth muscle cells (SMCs). However, in the present investigation surface SMCs of the mouse isolated vas deferens were poorly electrically coupled, with input resistances (176+/-18 MOmega, range: 141-221 MOmega, n=4) similar to those of dissociated cells. Furthermore, the amplitude of evoked EJPs was more variable in surface compared with deeper SMCs (F test, F=17.4, P<0.0001). Using simultaneous electrophysiology and confocal microscopy to investigate these poorly-coupled cells, it is shown that alpha-latrotoxin-stimulated sEJPs correlate, in timing (median delay ranged from -30 to -57 ms, P<0.05 in all experiments, n=5) and amplitude (Pearson product moment correlation, rho>0.55 and P<0.001), with purinergic neuroeffector Ca2+ transients (NCTs) in SMCs. The temporal correlation between sEJPs of widely ranging amplitude with NCTs in the impaled SMC demonstrates that all sEJPs could arise from neurotransmitter action on the impaled cell and that the skewed distribution of sEJPs can be explained by the variable effect of packets of ATP on a single SMC. The amplitude correlation of sEJPs and NCTs argues against the attenuation of electrical signal amplitude along the length of a single SMC. The skewed sEJP amplitude distribution arising from neurotransmitter release on single SMCs is consistent with a broad neurotransmitter packet size distribution at sympathetic neuroeffector junctions.
Asunto(s)
Potenciales de la Membrana/fisiología , Miocitos del Músculo Liso/fisiología , Unión Neuromuscular/fisiología , Conducto Deferente/citología , Adenosina Trifosfato/metabolismo , Animales , Señalización del Calcio/efectos de los fármacos , Señalización del Calcio/fisiología , Relación Dosis-Respuesta Inmunológica , Impedancia Eléctrica , Estimulación Eléctrica/métodos , Técnicas In Vitro , Masculino , Potenciales de la Membrana/efectos de los fármacos , Potenciales de la Membrana/efectos de la radiación , Ratones , Ratones Endogámicos BALB C , Microscopía Confocal/métodos , Unión Neuromuscular/efectos de los fármacos , Venenos de Araña/farmacología , Factores de TiempoRESUMEN
In 1969, Paton and Vizi described the inhibitory actions of noradrenaline on acetylcholine release from the innervation of the guinea-pig ileum longitudinal muscle. They concluded "that acetylcholine output by the nervous networks of the longitudinal strip is under the normal control of the sympathetic by a species of presynaptic inhibition mediated by <==> receptors". This work was carried out in the Pharmacology Department at Oxford University. Clearly, a period in the 'Dreaming Spires' of Oxford sufficiently inspired Sylvester to take up a life long career in scientific research. He has published more than 300 papers on a wide range of topics but clearly has a strong interest in neurotransmitter release mechanisms and recently, non-synaptic interactions between neurons. It seems fitting therefore to write a brief review on the continuing studies on neurotransmitter release mechanisms in sympathetic neurons in a volume honoring the now distinguished Professor Vizi.
Asunto(s)
Neuronas/fisiología , Neurotransmisores/fisiología , Sistema Nervioso Simpático/fisiología , Animales , Potenciales Postsinápticos Excitadores , Masculino , Neurotransmisores/metabolismo , Sistema Nervioso Simpático/citología , Sistema Nervioso Simpático/metabolismo , Conducto Deferente/inervaciónRESUMEN
1. Previous studies have demonstrated that chronic pre-synaptic inhibition of transmitter release by morphine evokes a counter-adaptive response in the sympathetic nerve terminals that manifests itself as an increase in transmitter release during acute withdrawal. In the present study we examined the possibility that other pre-synaptically acting drugs such as clonidine also evoke a counter-adaptive response in the sympathetic nerve terminals. 2. In chronically saline treated (CST) preparations, clonidine (0.5 microM) completely abolished evoked transmitter release from sympathetic varicosities bathed in an extracellular calcium concentration ([Ca(2+)](o)) of 2 mM. The inhibitory effect of clonidine was reduced by increasing [Ca(2+)](o) from 2 to 4 mM and the stimulation frequency from 0.1 to 1 Hz. 3. The nerve terminal impulse (NTI) was not affected by concentrations of clonidine that completely abolished evoked transmitter release. 4. Sympathetic varicosities developed a tolerance to clonidine (0.5 microM) following 7-9 days of chronic exposure to clonidine. 5. Acute withdrawal of preparations following chronic clonidine treatment (CCT) resulted in a significant (P < 0.005) enhancement of neurotransmitter release (3.75 times) above control levels observed in CST preparations. 6. The present findings demonstrate an enhancement of neurotransmitter release from sympathetic varicosities following acute withdrawal from chronic clonidine treatment.
Asunto(s)
Agonistas alfa-Adrenérgicos/farmacología , Clonidina/farmacología , Neurotransmisores/metabolismo , Conducto Deferente/efectos de los fármacos , Animales , Calcio/farmacología , Relación Dosis-Respuesta a Droga , Cobayas , Técnicas In Vitro , Masculino , Unión Neuromuscular/efectos de los fármacos , Unión Neuromuscular/fisiología , Fibras Simpáticas Posganglionares/efectos de los fármacos , Fibras Simpáticas Posganglionares/metabolismo , Conducto Deferente/inervación , Conducto Deferente/metabolismoRESUMEN
1. Action potential-evoked Ca(2+) transients in postganglionic sympathetic axon bundles in mouse vas deferens have been characterized using confocal microscopy and Ca(2+) imaging. 2. Axonal Ca(2+) transients were tetrodotoxin sensitive. The amplitude depended on both the frequency of stimulation and the number of stimuli in a train. 3. Removal of extracellular Ca(2+) abolished the Ca(2+) transient. Cd(2+)(100 microM) inhibited the Ca(2+) transient by 78 +/- 10 %. The N-type Ca(2+) channel blocker omega-conotoxin GVIA (0.1 microM) reduced the amplitude by -35 +/-4 %, whereas nifedipine (10 microM; L-type) and omega-conotoxin MVIIC (0.1 microM; P/Q type) were ineffective. 4. Caffeine (10 mM), ryanodine (10 microM), cyclopiazonic acid (30 microM) or CCCP (10 microM) had no detectable effects. 5. Blockade of large and small conductance Ca(2+)-dependent K+ channels with iberiotoxin (0.1 microM) and apamin (1 microM), respectively, or Ca(2+)-dependent Cl(-) channels by niflumic acid (100 microM) did not alter Ca(2+) transients. 6. In contrast, the non-specific K+ channel blockers tetraethylammonium (10 mM) and 4-aminopyridine (10 mM) markedly increased the amplitude of the Ca(2+) transient. Blockade of delayed rectifiers and A-like K+ channels, by tityustoxin-K (alpha) (0.1 microM) and pandinustoxin-K (alpha) (10 nM), respectively, also increased the Ca(2+) transient amplitude. 7. Thus, Ca(2+) transients are evoked by Na(+)-dependent action potentials in axons. These transients originate mainly from Ca(2+) entry through voltage-dependent Ca(2+) channels (80 % Cd(2+) sensitive of which 40 % was attributable to N-type). Twenty per cent of the Ca(2+) transient was not due to Ca(2+) entry through voltage-gated Ca(2+) channels. Intracellular stores and mitochondria were not involved in the generation of the transient. Ca(2+) transients are modulated by A-like K+ channels and delayed rectifiers (possibly K(V)1.2) but not by Ca(2+)-activated ion channels.
Asunto(s)
Axones/fisiología , Calcio/fisiología , Ganglios/fisiología , Sistema Nervioso Simpático/fisiología , Potenciales de Acción/fisiología , Animales , Canales de Calcio/metabolismo , Canales de Cloruro/antagonistas & inhibidores , Estimulación Eléctrica/métodos , Electrofisiología , Membranas Intracelulares/metabolismo , Activación del Canal Iónico , Ionomicina/farmacología , Ionóforos/farmacología , Ratones , Ratones Endogámicos BALB C , Bloqueadores de los Canales de Potasio/farmacología , Canales de Potasio/fisiología , Tetrodotoxina/farmacología , Factores de TiempoRESUMEN
While nicotine is known to act at neuronal nicotinic acetylcholine receptors (nAChRs) to facilitate neurotransmitter release, the mechanisms underlying this action are poorly understood. Some of its effects are known to be mediated by presynaptic receptors. In the mouse vas deferens nicotine (10-30 microM) transiently increased the force of neurogenic contraction by 135+/-25%, increased the amplitude of excitatory junction potentials by 74+/-6% and increased the frequency of spontaneous excitatory junction potentials in four out of six preparations. Confocal microscopy and the calcium indicator Oregon Green 488 BAPTA-1 dextran were used to measure calcium concentration changes in the nerve terminals. Nicotine did not affect the action potential-evoked calcium transient but instead triggered small, random fluctuations ("calcium spikes") in intra-varicosity calcium concentrations at an average frequency of 0.09+/-0.02 Hz. These were insensitive to tetrodotoxin at a concentration that blocked action-potential evoked calcium transients (300 nM). They were abolished by the nAChR blocker hexamethonium (100 microM) and by both ryanodine (100 microM) and caffeine (3 mM), agents that modify calcium release from intracellular stores. We propose a novel mechanism whereby nicotine's action at nAChRs triggers calcium-induced calcium release from a ryanodine-sensitive calcium store in nerve terminals. This primes neurotransmitter release mechanisms and enhances both spontaneous and action potential-evoked neurotransmitter release.
Asunto(s)
Señalización del Calcio/efectos de los fármacos , Calcio/metabolismo , Líquido Intracelular/efectos de los fármacos , Unión Neuromuscular/efectos de los fármacos , Nicotina/farmacología , Terminales Presinápticos/efectos de los fármacos , Receptores Nicotínicos/efectos de los fármacos , Antagonistas Adrenérgicos alfa/farmacología , Animales , Cafeína/farmacología , Canales de Calcio/efectos de los fármacos , Canales de Calcio/metabolismo , Señalización del Calcio/fisiología , Estimulación Eléctrica , Potenciales Postsinápticos Excitadores/efectos de los fármacos , Potenciales Postsinápticos Excitadores/fisiología , Colorantes Fluorescentes/farmacocinética , Hexametonio/farmacología , Líquido Intracelular/metabolismo , Masculino , Ratones , Ratones Endogámicos BALB C , Contracción Muscular/efectos de los fármacos , Contracción Muscular/fisiología , Músculo Liso/efectos de los fármacos , Músculo Liso/inervación , Músculo Liso/fisiología , Unión Neuromuscular/metabolismo , Unión Neuromuscular/ultraestructura , Antagonistas Nicotínicos/farmacología , Compuestos Orgánicos , Prazosina/farmacología , Terminales Presinápticos/metabolismo , Terminales Presinápticos/ultraestructura , Receptores Nicotínicos/metabolismo , Rianodina/farmacología , Fibras Simpáticas Posganglionares/citología , Fibras Simpáticas Posganglionares/efectos de los fármacos , Fibras Simpáticas Posganglionares/metabolismo , Tetrodotoxina/farmacología , Conducto Deferente/efectos de los fármacos , Conducto Deferente/inervación , Conducto Deferente/fisiologíaRESUMEN
An electropharmacological analysis of the type(s) of calcium channel controlling neurotransmitter release in preganglionic sympathetic nerve terminals in the guinea-pig anterior pelvic ganglion has been carried out. Conventional intracellular recording techniques were used to record excitatory postsynaptic potentials as a measure of neurotransmitter release. Excitatory postsynaptic potentials were abolished by hexamethonium (30-100 microM) and are therefore mediated by acetylcholine acting at nicotinic receptors. In studies of more than 150 cells, the N-type calcium channel blocker omega-conotoxin GVIA (100-300 nM) failed to block the initiation of the nerve impulse by the excitatory postsynaptic potential. In single-cell studies, omega-conotoxin GVIA (1 microM) sometimes altered the configuration of the excitatory postsynaptic potential/cell body nerve action potential complex, but on only one occasion was the excitatory postsynaptic potential reduced below the threshold required to initiate the action potential. Nifedipine (10 microM), omega-agatoxin IVA (100 nM) and omega-conotoxin MVIIC (300 nM), applied alone or in combination with omega-conotoxin GVIA (300 nM), were also ineffective. However, excitatory postsynaptic potentials evoked by trains of stimuli (0.1-0.5 Hz) were markedly reduced or abolished by the non-specific calcium channel blocker omega-grammotoxin SIA (300 nM). When trains of stimuli were delivered at higher frequencies (4 Hz), the block induced by omega-grammotoxin SIA could be overcome, and excitatory postsynaptic potentials were able to initiate action potentials even when omega-conotoxin GVIA, omega-agatoxin IVA and omega-conotoxin MVIIC were also present. The calcium channel(s) controlling acetylcholine release was (were) blocked by low concentrations of cadmium ions (30 microM) at all stimulation frequencies studied (0.1-50 Hz). Thus, the dominant calcium channels controlling acetylcholine release in sympathetic ganglia are not the L, N, P or Q types. At low frequencies of stimulation, omega-grammotoxin SIA-sensitive calcium channels play a dominant role in acetylcholine release, but at higher stimulation frequencies yet another pharmacologically distinct calcium channel (or subtype) supports neurotransmitter release.
Asunto(s)
Acetilcolina/metabolismo , Bloqueadores de los Canales de Calcio/farmacología , Canales de Calcio/fisiología , Potenciales Postsinápticos Excitadores/fisiología , Ganglios Simpáticos/fisiología , Neuronas/fisiología , omega-Conotoxinas , Animales , Cadmio/farmacología , Canales de Calcio Tipo N/fisiología , Estimulación Eléctrica , Potenciales Postsinápticos Excitadores/efectos de los fármacos , Ganglios Simpáticos/efectos de los fármacos , Cobayas , Hexametonio/farmacología , Técnicas In Vitro , Masculino , Neuronas/efectos de los fármacos , Nifedipino/farmacología , Técnicas de Placa-Clamp , Péptidos/farmacología , Péptidos Cíclicos/farmacología , Receptores Nicotínicos/fisiología , omega-Conotoxina GVIA/farmacologíaRESUMEN
1. The effects of Ca2+ concentration and Ca2+ channel blockers on noradrenaline (NA) and adenosine 5'-triphosphate (ATP) release from postganglionic sympathetic nerves have been investigated in rat tail arteries in vitro. Intracellularly recorded excitatory junction potentials (e.j.ps) were used as a measure of ATP release and continuous amperometry was used to measure NA release. 2. Varying the extracellular Ca2+ concentration similarly affected the amplitudes of e.j.ps and NA-induced oxidation currents evoked by trains of ten stimuli at 1 Hz. 3. The N-type Ca2+ blocker, omega-conotoxin GVIA (omega-CTX GVIA, 0.1 microM) reduced the amplitudes of both e.j.ps (evoked by trains of ten stimuli at 1 Hz) and NA-induced oxidation currents (evoked by trains of ten stimuli at 1 Hz and 50 stimuli at 10 Hz) by about 90%. 4. The omega-CTX GVIA resistant e.j.ps and NA-induced oxidation currents evoked by trains of 50 stimuli at 10 Hz were abolished by the non-selective Ca2+ channel blocker, Cd2+ (0.1 mM), and were reduced by omega-conotoxin MVIIC (0.5 microM) and omega-agatoxin IVA (40 nM). 5. Nifedipine (10 microm) had no inhibitory effect on omega-CTX GVIA resistant e.j.ps and NA-induced oxidation currents. 6. Thus both varying Ca2+ concentration and applying Ca2+ channel blockers results in similar effects on NA and ATP release from postganglionic sympathetic nerves. These findings are consistent with the hypothesis that NA and ATP are co-released together from the sympathetic nerve terminals.
Asunto(s)
Adenosina Trifosfato/metabolismo , Arterias/efectos de los fármacos , Bloqueadores de los Canales de Calcio/farmacología , Calcio/farmacología , Norepinefrina/metabolismo , Transmisión Sináptica/efectos de los fármacos , omega-Conotoxinas , Animales , Arterias/inervación , Arterias/metabolismo , Calcio/metabolismo , Relación Dosis-Respuesta a Droga , Electrofisiología , Potenciales Evocados/efectos de los fármacos , Femenino , Técnicas In Vitro , Potenciales de la Membrana/efectos de los fármacos , Nifedipino/farmacología , Péptidos/farmacología , Ratas , Ratas Wistar , Sistema Nervioso Simpático/efectos de los fármacos , Sistema Nervioso Simpático/metabolismo , Cola (estructura animal) , omega-Conotoxina GVIARESUMEN
The effects of sympathetic nerve stimulation on different cutaneous arteries were examined in arteries isolated from guinea-pig ears, by measuring membrane potential changes in smooth muscle cells in response to electrical field stimulation. Resting membrane potential (RMP) was similar in proximal (main ear artery) and distal (3rd or 4th branch order) cutaneous arteries (mean -71 mV). Single stimuli evoked excitatory junction potentials (EJPs) in all arteries. The EJPs in proximal arteries were twice the amplitude, and the time constant of EJP decay was almost half the value, compared with distal cutaneous arteries. EJP amplitude was reduced by > 90% by suramin (30 microM) or alpha,beta,methylene-ATP (alpha,beta,m-ATP)(1 microM) in all proximal, and most distal arteries. Residual responses in distal arteries were resistant to tetrodotoxin. The N-type calcium channel blocker, omega-conotoxin GVIA (30 nM), reduced EJP amplitude by 70-100% in both proximal and distal arteries. Successive EJPs evoked by trains of stimuli at 1 to 5 Hz were depressed in amplitude in proximal arteries, but showed facilitation in distal arteries. EJP depression in proximal arteries was reversed to facilitation by the alpha2-adrenoceptor antagonist, yohimbine (30 nM). Trains of stimuli delivered at 10-20 Hz produced summation of EJPs and active membrane responses in 30% of proximal arteries. Active responses were never detected in distal arteries. Slow depolarizations following the EJPs were detected in most arteries after trains of stimuli, and were abolished by prazosin (0.3 microM) or omega-conotoxin GVIA (30 nM). The density of the perivascular plexus of axons innervating proximal arteries, demonstrated with catecholamine fluorescence histochemistry, was twice that in distal cutaneous arteries. These regional differences in sympathetic neurotransmission suggest that cutaneous vasoconstriction in response to thermoregulatory stimuli, which occurs predominantly in distal cutaneous segments, is likely to be qualitatively different from cutaneous vasoconstriction of proximal arteries in response to other physiological stimuli.
Asunto(s)
Piel/irrigación sanguínea , Piel/inervación , Sistema Nervioso Simpático/fisiología , Transmisión Sináptica/fisiología , Antagonistas Adrenérgicos alfa/farmacología , Animales , Arterias/fisiología , Bloqueadores de los Canales de Calcio/farmacología , Canales de Calcio/fisiología , Oído , Femenino , Cobayas , Masculino , Potenciales de la Membrana/efectos de los fármacos , Potenciales de la Membrana/fisiología , Péptidos/farmacología , Receptores Adrenérgicos alfa/fisiología , Receptores Purinérgicos/fisiología , Sistema Nervioso Simpático/química , Vasoconstricción/efectos de los fármacos , Vasoconstricción/fisiología , Yohimbina/farmacología , omega-Conotoxina GVIARESUMEN
1 Intracellular recording techniques were used to study neurotransmitter release mechanisms in postganglionic sympathetic nerve terminals in the guinea-pig isolated vas deferens. 2 Recently, a component of action potential-evoked release which is insensitive to high concentrations of the selective N-type calcium channel blocker omega-conotoxin GVIA termed 'residual release' has been described. Under these conditions, release of the neurotransmitter ATP evoked by trains of low frequency stimuli is abolished, but at higher frequencies a substantial component of release is revealed. 3 'Residual release' was studied with trains of 5 or 10 stimuli at stimulation frequencies of 10, 20 and 50 Hz. The alpha2-adrenoceptor agonist clonidine (30-100 nM) inhibited 'residual release', the degree of inhibition being most marked at the beginning of a train. 4 The alpha2-adrenoceptor antagonist yohimbine (1 microM) induced a marked increase in 'residual release' which was dependent on both the frequency of stimulation and the number of stimuli in a train. 5 Prostaglandin E2 (30 nM) and neuropeptide Y (100 nM) caused a rapid inhibition of 'residual release' at all stimulation frequencies examined. 6 4-Aminopyridine (100 microM) induced a powerful potential of 'residual release' and could reverse the inhibition of omega-conotoxin GVIA. 7 'Residual release' was modulated through presynaptic alpha2-adrenoceptors suggesting that (i) residual release of ATP is subject to alpha-autoinhibition through the co-release of noradrenaline, (ii) noradrenaline release can be triggered by calcium channels other than the N-type and (iii) when presynaptic receptors are activated, inhibition of transmitter release can occur by mechanisms other than modulation of calcium-entry through N-type calcium channels in postganglionic sympathetic nerves. Prostaglandin E2 and neuropeptide Y also modulated neurotransmitter release.
Asunto(s)
Bloqueadores de los Canales de Calcio/farmacología , Neurotransmisores/metabolismo , Péptidos/farmacología , Receptores Adrenérgicos alfa 2/efectos de los fármacos , Receptores Adrenérgicos alfa 2/fisiología , Fibras Simpáticas Posganglionares/efectos de los fármacos , Fibras Simpáticas Posganglionares/metabolismo , Conducto Deferente/inervación , 4-Aminopiridina/farmacología , Agonistas alfa-Adrenérgicos/farmacología , Antagonistas Adrenérgicos alfa/farmacología , Animales , Clonidina/farmacología , Dinoprostona/farmacología , Cobayas , Técnicas In Vitro , Masculino , Ratones , Neuropéptido Y/farmacología , Yohimbina/farmacología , omega-Conotoxina GVIAAsunto(s)
Fibras Adrenérgicas/fisiología , Canales de Calcio/fisiología , Calcio/metabolismo , Neuronas/fisiología , Neurotransmisores/metabolismo , Canal Liberador de Calcio Receptor de Rianodina/fisiología , Potenciales de Acción , Adenosina Trifosfato/fisiología , Animales , Estimulación Eléctrica , Cobayas , Masculino , Norepinefrina/fisiología , Péptidos/farmacología , Rianodina/farmacología , Conducto Deferente/inervación , omega-Conotoxina GVIARESUMEN
1. The effects of prejunctional beta-adrenoceptor activation on electrically evoked noradrenaline (NA) and adenosine 5'-triphosphate (ATP) were studied by use of continuous amperometry and conventional intracellular recording techniques. Excitatory junction potentials (e.j.ps) were used as a measure of ATP release, and NA-induced slow depolarizations and oxidation currents as measures of NA release, from postganglionic sympathetic nerves innervating the rat tail artery in vitro. 2. Isoprenaline (0.1 microM) increased the amplitude of e.j.ps, slow depolarizations and oxidation currents evoked by short trains of stimuli at 1 to 4 Hz. The facilitatory effect of isoprenaline on e.j.ps and oxidation currents was most pronounced on responses evoked by the first stimulus in a train. 3. Isoprenaline (0.1 microM) did not detectably alter the amplitude-frequency distribution of spontaneous e.j.ps. 4. The facilitatory effect of isoprenaline on e.j.ps, slow depolarizations and oxidation currents was abolished by the beta-adrenoceptor antagonist, propranolol (0.1 microM). Propranolol alone had no effect on e.j.ps, slow depolarizations or oxidation currents. 5. Thus, activation of prejunctional beta-adrenoceptors increases the release of both NA and ATP from postganglionic sympathetic nerves. The findings are consistent with the hypothesis that NA and ATP are released from the same population of nerve terminals and presumably from the same vesicles.
Asunto(s)
Adenosina Trifosfato/metabolismo , Arterias/inervación , Norepinefrina/metabolismo , Receptores Adrenérgicos beta/fisiología , Sistema Nervioso Simpático/metabolismo , Cola (estructura animal)/irrigación sanguínea , Agonistas Adrenérgicos beta/farmacología , Animales , Arterias/fisiología , Electroquímica , Isoproterenol/farmacología , Masculino , Potenciales de la Membrana/efectos de los fármacos , Propranolol/farmacología , Ratas , Sistema Nervioso Simpático/fisiología , Simpatomiméticos/farmacologíaRESUMEN
1. Intracellular recording techniques were used to study neurotransmitter release mechanisms in postganglionic sympathetic nerve terminals of the rat isolated anococcygeus muscle. 2. Low concentrations of the N-type calcium channel blocker omega-conotoxin GVIA (omega-CgTX GVIA) irreversibly abolished excitatory junction potentials (EJPs) evoked by trains of < or = five stimuli at 10 Hz. When the frequency of stimulation was increased (10-50 Hz) trains of stimuli evoked EJPs even in the presence of 1 microM omega-CgTX GVIA. We have termed this omega-CgTX GVIA-resistant release 'residual release'. EJP amplitude in the presence of omega-CgTX GVIA depended on both the frequency and number of stimuli in a train. 3. Residual release was inhibited by the P-type calcium channel blocker omega-agatoxin IVA (100 nM). However, even in the presence of both toxins, longer trains of stimuli could still evoke neurotransmitter release. 4. Residual release was abolished by omega-conotoxin MVIIC and by the non-specific calcium channel antagonist omega-grammotoxin SIA. Therefore, it would appear that a heterogeneous population of calcium channels is involved in mediating neurotransmitter release from these sympathetic nerve terminals.
Asunto(s)
Canales de Calcio/metabolismo , Neurotransmisores/metabolismo , Terminales Presinápticos/metabolismo , omega-Conotoxinas , Animales , Bloqueadores de los Canales de Calcio/farmacología , Canales de Calcio/efectos de los fármacos , Potenciales Evocados/efectos de los fármacos , Masculino , Venenos de Moluscos/farmacología , Péptidos/farmacología , Péptidos Cíclicos/farmacología , Terminales Presinápticos/efectos de los fármacos , Ratas , Ratas Wistar , Venenos de Araña/farmacología , omega-Agatoxina IVA , omega-Conotoxina GVIARESUMEN
1. Intracellular and focal extracellular recording techniques were used to study neurotransmitter release mechanisms in postganglionic sympathetic nerve terminals in the guinea-pig isolated vas deferens. 2. High concentrations of the selective N-type calcium channel blocker omega-conotoxin GVIA abolished the release of the neurotransmitter ATP evoked by trains of low-frequency stimuli. However, in the presence of high concentrations of the blocker, a 'residual release' persisted at higher frequencies. 3. Residual release was dependent on calcium entry through a pharmacologically distinct voltage-dependent calcium channel. 4. Residual release was inhibited by ryanodine in a use- and time-dependent manner and this inhibitory effect was potentiated by caffeine. The inhibitory effect of ryanodine on residual release was reversed by 4-aminopyridine. 5. These findings indicate that calcium-induced calcium released from intraneuronal stores plays an important role in action potential-evoked neurotransmitter release mechanisms in postganglionic sympathetic nerve terminals.
Asunto(s)
Calcio/metabolismo , Neurotransmisores/metabolismo , Rianodina/farmacología , Sistema Nervioso Simpático/metabolismo , Adenosina Trifosfato/farmacología , Animales , Bloqueadores de los Canales de Calcio/farmacología , Potenciales Evocados/efectos de los fármacos , Cobayas , Masculino , Venenos de Moluscos/farmacología , Péptidos/farmacología , Conducto Deferente/inervación , omega-Conotoxina GVIARESUMEN
1. The effects of prostaglandin E1 (PGE1) and E2 (PGE2) on postjunctional electrical activity in the guinea-pig vas deferens evoked by sympathetic nerve stimulation were investigated using both intracellular and focal extracellular recording techniques in vitro. 2. Bath application of PGE1 (1-100 nM) or PGE2 (0.1-100 nM) concentration-dependently inhibited the amplitudes of all excitatory junction potentials (e.j.ps) evoked during short trains of stimuli (10 stimuli at 1 Hz). Increasing the duration of nerve stimulation (100 stimuli at 1 Hz) did not overcome this inhibitory effect. At these concentrations PGE1 and PGE2 were without any apparent inhibitory effect on the amplitudes of spontaneous e.j.ps. 3. Local application of PGE1 (10-100 nM) or PGE2 (10-30 nM) markedly reduced the frequency of occurrence of excitatory junction currents (e.j.cs) evoked by trains of 20-100 stimuli at 1 to 4 Hz without changing the amplitudes of spontaneous e.j.cs or the configuration of the nerve terminal impulse. 4. In the presence of PGE1 or PGE2, raising the frequency of stimulation (from 1 to 4 Hz), increased the likelihood of e.j.c. occurrence. 5. The postjunctional electrical activity recorded in the guinea-pig vas deferens is believed to be due to ATP released from the sympathetic nerve endings. Thus the present study demonstrates that both PGE1 and PGE2 powerfully inhibit quantal ATP release in the guinea-pig vas deferens.
Asunto(s)
Alprostadil/farmacología , Dinoprostona/farmacología , Potenciales de la Membrana/efectos de los fármacos , Conducto Deferente/efectos de los fármacos , Adenosina Trifosfato/metabolismo , Animales , Cobayas , Técnicas In Vitro , Cinética , MasculinoRESUMEN
Intracellular recording techniques were used to study neurotransmitter release in the guinea-pig isolated vas deferens. Low concentrations of the irreversible and selective N-type calcium channel blocker omega-conotoxin GVIA have previously been shown to block excitatory junction potentials evoked by low frequencies (< or = 1 Hz) of nerve stimulation. Here we report a component of action potential-evoked release which is insensitive to high concentrations of omega-conotoxin GVIA. We have termed this component "residual release" and show (i) it is positively frequency-dependent, (ii) its magnitude is dependent on both the train length and interval between trains, (iii) "residual release" can be modulated through prejunctional alpha 2-adrenoceptors and (iv) "residual release" is insensitive to many calcium entry blockers but abolished by omega-grammotoxin SIA and cadmium ions. Although noradrenaline is released by nerve action potentials, residual excitatory junction potentials were abolished by alpha,beta-methylene-ATP and therefore resulted entirely from the actions of neuronally released ATP acting through postjunctional P2x-purinoceptors. The results suggest that calcium entry through a novel, pharmacologically uncharacterized voltage-dependent calcium channel is responsible for "residual release" in sympathetic nerve terminals. It seems that in response to single or short trains of nerve action potentials, N-type calcium channels dominate the release process. However, at higher frequencies other voltage-dependent calcium channels are recruited and these may have an important role to play in triggering the mechanisms underlying frequency-dependent facilitation.
Asunto(s)
Ganglios Simpáticos/metabolismo , Terminaciones Nerviosas/metabolismo , Neurotransmisores/metabolismo , Péptidos/farmacología , Adenosina Trifosfato/análogos & derivados , Adenosina Trifosfato/farmacología , Animales , Calcio/metabolismo , Bloqueadores de los Canales de Calcio/farmacología , Estimulación Eléctrica , Cobayas , Masculino , Péptidos Cíclicos/farmacología , Yohimbina/farmacología , omega-Conotoxina GVIARESUMEN
1. A focal extracellular suction electrode was used to investigate the contributions of K+ and Ca2+ currents to the nerve impulse recorded from sympathetic nerve terminals innervating the guinea-pig vas deferens in vitro. 2. Perfusing the electrode with Cd2+ (0.1-0.5 mM) had little effect on the configuration of the nerve impulse. 3. Perfusing the electrode with Ba2+ (1-3 mM) caused the appearance of a second negative-going component of the nerve impulse. Local application of Cd2+ (0.1 mM) had little affect on this component of the nerve impulse. 4. Perfusing the electrode with 4-aminopyridine (4-AP) and/or tetraethylammonium (TEA) caused the appearance of a second negative-going component of the nerve impulse. This component has been termed the late negative-going component (LNC). 5. The LNC produced by local application of 1 mM 4-AP and 10 mM TEA was not changed when the solution perfusing the electrode contained no added Ca2+, 10 mM Ca2+ or omega-conotoxin GVIA (0.1 microM). Perfusion of the electrode with Cd2+ (0.1 mM) reduced the amplitude and slowed the time course of the LNC. 6. The LNC was markedly inhibited when the organ bath was perfused with TEA (10 mM) or 4-AP and TEA (1 and 10 mM, respectively). In some experiments the LNC was completely abolished. 7. The LNC was reduced in amplitude and slowed in time course when the solution perfusing the organ bath contained no added Ca2+. A similar effect on the LNC was observed when the solution perfusing the organ bath contained omega-conotoxin GVIA (0.1 microM), charybdotoxin (0.05 microM) or low concentrations of TEA (0.3-1 mM) or Ba2+ (10-500 microM). 8. Bath application of the alpha 2-adrenoceptor agonist clonidine (0.1-0.3 microM) did not detectably change the LNC. 9. The results demonstrate that the LNC produced by the local application of K+ blockers is due primarily to K+ efflux from sites outside the recording electrode and that a part of the change in conductance that underlies this component is due to opening of Ca(2+)-activated K+ channels. The failure to detect an effect of clonidine on the LNC suggests that activation of presynaptic alpha 2-adrenoceptors does not change either the K+ or the Ca2+ conductance of the nerve terminals.