RESUMEN
We have designed, built, and tested a climate-controlled, radiation-shielded incubator cabinet for the purpose of analyzing the effects of low-dose x-ray radiation on biological tissues and cell cultures. Bremsstrahlung x rays incident on exchangeable fluorescence plates produce strong, quasi-monochromatic radiation directed toward a small container of biological samples. The x-ray source, sample, and detector are enclosed in an incubator-maintaining the optimal environment for biological samples to increase longevity to a maximum of 72 h. To demonstrate the capabilities of the setup, an example experiment is presented. Rat vascular smooth muscle cell growth was observed after irradiation with characteristic x rays of iron, copper, and calcium to impart doses of 2 mGy each. Cultures show significant spectrum dependent increases in cell number over controls at 48 h after irradiation. The experiment lends credence to the efficacy of the apparatus and shows promise for future low-dose bio-radiation studies.
