PCR primer design for mitochondrial cox-1 gene from Clinostomum complanatum towards diagnosis.

Metacercariae of Clinostomum Leidy, 1856 are frequently encountered in freshwater fish. Clinostomum complanatum is a digenetic zoonotic parasite harbouring the intestine and body cavity of the fishes. 19 human incidences of Clinostomum complanatum infection have been reported to cause pharyngitis and lacramalitis from Japan, Thailand and Korea. Hence, adequate yet effective diagnosis is an issue. Designing primers used in the amplification of genes with adequate specificity and efficiency is of help in diagnosis. Hence, we describe primer design for cox-1 gene from the helminth parasite, Clinostomum complanatum parasitizing the intestine of fish Channastriata (Snakehead murrel). Thus, these designed primers set will be of further use in the wet lab for amplification of concerned gene or DNA fragment.

Modification of structure and physicochemical properties of buckwheat and oat starch by γ-irradiation.

Effects of gamma irradiation on the physicochemical, rheological and thermal properties of the buckwheat and oat starch were studied. Results revealed that the transition temperature and enthalpy of gelatinization of starch decreased with increase in irradiation dose. Pasting properties (peak, trough, setback, pasting temperature and final viscosity) of both the starches were decreased by gamma irradiation. Gamma irradiation also resulted in the reduction in the per cent crystallinity of the starches. Apparent amylose content and swelling index decreased with increase in dose. However, solubility index was increased by gamma irradiation. No surface fissures were seen in irradiated starches. FTIR spectra pattern did not change by gamma irradiation.