Supplementation of Crataegi fructus alleviates functional dyspepsia and restores gut microbiota in mice.

Introduction: Functional dyspepsia (FD), also known as non-ulcerative dyspepsia, is a common digestive system disorder. Methods: In this study, an FD model was established using hunger and satiety disorders combined with an intraperitoneal injection of L-arginine. Indices used to evaluate the efficacy of hawthorn in FD mice include small intestinal propulsion rate, gastric residual rate, general condition, food intake, amount of drinking water, gastric histopathological examination, and serum nitric oxide (NO) and gastrin levels. Based on the intestinal flora and their metabolites, short-chain fatty acids (SCFAs), the mechanism of action of Crataegi Fructus (hawthorn) on FD was studied. The fecal microbiota transplantation test was used to verify whether hawthorn altered the structure of the intestinal flora. Results: The results showed that hawthorn improved FD by significantly reducing the gastric residual rate, increasing the intestinal propulsion rate, the intake of food and drinking water, and the levels of gastrointestinal hormones. Simultaneously, hawthorn elevated substance P and 5-hydroxytryptamine expression in the duodenum, reduced serum NO levels, and increased vasoactive intestinal peptide expression in the duodenum. Notably, hawthorn increased the abundance of beneficial bacteria and SCFA-producing bacteria in the intestines of FD mice, decreased the abundance of conditional pathogenic bacteria, and significantly increased the SCFA content in feces. Discussion: The mechanism by which hawthorn improves FD may be related to the regulation of intestinal flora structure and the production of SCFAs.

Arsenic field test kits based on solid-phase fluorescence filter effect induced by silver nanoparticle formation.

Millions of people worldwide are affected by naturally occurring arsenic in groundwater. The development of a low-cost, highly sensitive, portable assay for rapid field detection of arsenic in water is important to identify areas for safe wells and to help prioritize testing. Herein, a novel paper-based fluorescence assay was developed for the on-site analysis of arsenic, which was constructed by the solid-phase fluorescence filter effect (SPFFE) of AsH3-induced the generation of silver nanoparticles (AgNPs) toward carbon dots. The proposed SPFFE-based assay achieves a low arsenic detection limit of 0.36 µg/L due to the efficient reduction of Ag+ by AsH3 and the high molar extinction coefficient of AgNPs. In conjunction with a smartphone and an integrated sample processing and sensing platform, field-sensitive detection of arsenic could be achieved. The accuracy of the portable assay was validated by successfully analyzing surface and groundwater samples, with no significant difference from the results obtained through mass spectrometry. Compared to other methods for arsenic analysis, this developed system offers excellent sensitivity, portability, and low cost. It holds promising potential for on-site analysis of arsenic in groundwater to identify safe well locations and quickly obtain output from the global map of groundwater arsenic.

Portable Pyrolysis-Point Discharge Optical Spectrometer for In Situ Plastic Polymer Identification by Coupling with Machine Learning.

Rapid and in situ identification of specific polymers is a challenging and crucial step in plastic recycling. However, conventional techniques continue to exhibit significant limitations in the rapid and field classification of plastic products, especially with the wide range of commercially available color polymers because of their large size, high energy consumption, and slow and complicated analysis procedures. In this work, a simple analytical system integrating a miniature and low power consumption (22.3 W) pyrolyzer (Pyr) and a low temperature, atmospheric pressure point discharge optical emission spectrometer (µPD-OES) was fabricated for rapidly identifying polymer types. Plastic debris is decomposed in the portable pyrolyzer to yield volatile products, which are then swept into the µPD-OES instrument for monitoring the optical emission patterns of the thermal pyrolysis products. With machine learning, five extensively used raw polymers and their consumer plastics were classified with an accuracy of ≥97.8%. Furthermore, the proposed method was applied to the identification of the aged polymers and plastic samples collected from a garbage recycling station, indicating its great potential for identification of environmentally weathered plastics. This portable Pyr-µPD-OES system provides a cost-effective tool for rapid and field identification of polymer types of recycled plastic for proper management and resource recycling.

Automatic and Matrix Interference-Free Acid-Base Titration by Coupling CO2 Vapor Generation with Microplasma Carbon Optical Emission Spectrometry.

Acid-base titration of complex samples is conducive to the rapid evaluation of the degree and risk of environmental pollution to some extent. However, the traditional titration methods usually suffer from serious interference. Herein, an automatic acid-base titration method coupling miniature point discharge optical emission spectrometry (µPD-OES) with CO2 vapor generation was described for the precise, sensitive, and matrix interference-free acid-base titration of complex samples, particularly those with high color intensity, salinity, and turbidity such as wastewater and soil samples. In this work, acid-base titration was carried out in a chemical vapor generator where CO2 was generated through the addition of HCl or NaHCO3, thus enabling efficient separation of CO2 from a complex matrix. The generated CO2 was subsequently swept into the miniaturized point discharge for excitation and further detection by µPD-OES, where the carbon atomic emission at 193.0 nm was monitored. According to the consumed volume and concentration of HCl, accurate and automatic measurements of OH-, CO32-, and HCO3- can be accomplished. The proposed method possesses a high sensitivity of µPD-OES for the detection of CO2 with a relative standard deviation of below 3.0%. Moreover, the proposed system not only retains several unique advantages of accuracy, simplicity, and elimination of the use of complicated, expensive, and high power-consumption instruments but also alleviates the color and turbid interference from complex samples such as dyeing wastewater samples, oilfield water samples, and soil samples. It retains a promising potential application for titration analysis of other samples such as sludge, sediment, and landfill leachate.

Solid Phase Photothermo-Induced Chemical Vapor Generation: A New Desorption Method for Mercury Analysis by High-Throughput 20-Fiber Direct Immersion Solid Phase Microextraction.

A simple solid phase photothermo-induced chemical vapor generation (SP-PT-CVG) is described and used as an environmentally friendly desorption method for the sensitive determination of mercury in water by direct immersion solid phase microextraction (DI-SPME) atomic fluorescence spectrometry (AFS). A DI-SPME array equipped with 20 nano-TiO2-coated tungsten fibers was employed to simultaneously preconcentrate mercury from 20 samples, enabling an extraction throughput of 40 samples per hour. Subsequently, the fibers were drawn from the sample solutions and inserted into an inner tube sealed in a specially designed UV lamp in turn for SP-PT-CVG to generate Hg0, which was swept to an AFS detector for its detection. It is worth noting that the tube served as both a vapor generator and a desorption chamber. This proof-of-concept study confirms the feasibility of solid phase CVG. Compared to conventional CVG carried out in the liquid phase, solid phase CVG not only retains the advantages of conventional CVG but also alleviates the matrix interference on vapor generation and preconcentrates analyte prior to vapor generation, improving analytical performance for liquid state samples. DI-SPME-SP-PT-CVG-AFS provides a limit of detection of 2.3 ng L-1 for mercury determination by AFS. In the proposed method, the combination of DI-SPME and SP-PT-CVG eliminates the tedious derivatization steps required in conventional headspace SPME, thus minimizing toxic reagent consumption and improving extraction throughput. The practicality of DI-SPME-SP-PT-CVG-AFS was evaluated by analyzing two different certified reference materials and river water samples with good spike recoveries (98-107%).

Paper-assisted ratiometric fluorescent sensors for on-site sensing of sulfide based on the target-induced inner filter effect.

Dissolved sulfide tends to species transformation and loss upon leaving the matrix, thus the development of a practical on-site determination of sulfide is crucial for environmental monitoring and human health. In this work, a novel paper-based ratiometric fluorescence sensor was developed for the field analysis of sulfide, which system was constructed by the inner filter effect (IFE) of CdS quantum dots (QDs) toward carbon dots (C-dots). Instead of an aqueous phase system, the conversion of sulfide to its hydride would induce the in-situ formation of CdS QDs on the paper, which acted as an energy acceptor to quench the emission of C-dots, leading to a variation of ratiometric fluorescence from blue to yellow with the increasing concentration of sulfide. Moreover, we proposed a smartphone-based fluorescence capture device integrated with a programmed Python program, accomplishing both color recognition and accurate detection of sulfide. Under the optimal condition, this ratiometric fluorescence sensor allowed for the on-site analysis of sulfide with a limit of detection of 0.05 µM. The accuracy of the sensor was validated via the successful field analysis of environmental water samples with satisfactory recoveries. Compared to other fluorescence methods used for sulfide analysis, this developed system retains the advantages of label-free, low-cost, ease of operation, and miniaturization, showing great potential for the measurement of sulfide on-site, as well as environmental monitoring.

Battery-Operated and Self-Heating Solid-Phase Microextraction Device for Field Detection and Long-Term Preservation of Mercury in Soil.

The application of headspace solid-phase microextraction (HS-SPME) for mercury preservation and detection still has several shortcomings, including the use of high-temperature desorption chamber, the consumption of expensive reagent (NaBEt4 or NaBPr4), and analyte loss during sample storage. Herein, a self-heating HS-SPME device using a gold-coated tungsten (Au@W) fiber was developed for the field detection of mercury in soil by miniature point discharge optical emission spectrometry (µPD-OES). Hg2+ was reduced to Hg0 with NaBH4 solution and then preconcentrated with the Au@W fiber. The adsorbed Hg0 could be rapidly desorbed by directly heating the fiber with a mini lithium battery and subsequently detected by µPD-OES. A limit of detection (LOD) of 0.008 mg kg-1 was obtained with a relative standard deviation (RSD) of 2.4%. The accuracy of the self-heating HS-SPME was evaluated by analyzing a soil certified reference material (CRM) and nine soil samples with satisfactory recoveries (86-111%). Compared to the conventional external heating method, the proposed method reduces desorption time and power consumption from 80 s and 60 W to 20 s and 2.5 W, respectively. Moreover, the self-heating device enables the µPD-OES system to remove the high-temperature desorption chamber, making it more compact and suitable for field analytical chemistry. Interestingly, the Au@W SPME fiber can be also used for the long-term preservation of mercury with a sample loss rate <5% after 30 days of storage at room temperature.

Rapid Testing of Δ9-Tetrahydrocannabinol and Its Metabolite On-Site Using a Label-Free Ratiometric Fluorescence Assay on a Smartphone.

Excessive consumption of Δ9-tetrahydrocannabinol (THC) severely endangers human health and has raised public safety concerns. However, its quantification by readily rapid tools with simplicity and low cost is still challenging. Herein, we found that a G-rich THC aptamer (THC1.2) can tightly bind to thioflavin T (ThT) with strong fluorescence, which would be specifically quenched in the presence of THC. Based on that, a label-free ratiometric fluorescent sensor for the sensing of THC and its metabolite (THC-COOH) based on THC1.2/ThT as a color emitter and red CdTe quantum dots as reference fluorescence was constructed. Notably, a transition of the fluorescent color of the ratiometric probe from green to red can be instantly observed upon the increased concentration of THC and THC-COOH. Furthermore, a portable smartphone-based fluorescence device integrated with a self-programmed Python program was fabricated and used to accomplish on-site monitoring of THC and THC-COOH within 5 min. Under optimized conditions, this ratiometric fluorescent sensor allowed for an instant response toward THC and its metabolite with considerable limits of detection of 97 and 254 nM, respectively. The established sensor has been successfully applied to urine and saliva samples and exhibited satisfactory recoveries (88-116%). This ratiometric fluorescent sensor can be used for the simultaneous detection of THC and THC-COOH with the advantages of rapidness, low cost, ease of operation, and portability, providing a promising strategy for on-site detection and facilitating law enforcement regulation and roadside control of THC.

Nozzle Electrode Point Discharge-Enhanced Oxidation and Portable Gas Phase Molecular Fluorescence Spectrometry for Sensitive Field Detection of Sulfide.

It is still a challenge to accurately determine dissolved sulfide due to its susceptibility to contamination and loss during transportation, storage, and analysis in the laboratory, thus highlighting the necessity for its sensitive field analysis. Herein, a robust nozzle electrode point discharge (NEPD) enhanced oxidation coupling with chemical vapor generation (CVG) is described for the highly efficient and flameless conversion of sulfide (S2-) to SO2. Subsequently, a portable and low-power consumption gas phase molecular fluorescence spectrometry (GP-MFS) was constructed for the highly selective and sensitive determination of the generated SO2 via detecting its molecular fluorescence excited by a zinc hollow cathode lamp. Under optimal conditions, a limit of detection (LOD) of 0.1 µM was obtained for dissolved sulfide with a relative standard deviation (RSD, n = 11) of 2.6%. The accuracy and practicability of the proposed method were validated by the analyses of two certified reference materials (CRMs) and several river and lake water samples with satisfactory recoveries of 99%-107%. This work confirms that NEPD enhanced oxidation is a low energy consumption yet highly efficient method for the flameless oxidation of hydrogen sulfide and thus is suitable for the easy field detection of dissolved sulfide in environmental water by CVG-GP-MFS.

A high-throughput atomic emission analyzer for simultaneous field detection of dissolved inorganic and organic carbon in seawater and lake water.

Dissolved inorganic carbon (DIC) and dissolved organic carbon (DOC) are two important indicators of global carbon cycle. However, there are no portable analyzers available to simultaneously accomplish high-throughput field detection of them in the same sample. Herein, a simple analyzer comprising a dual-mode reactor to accomplish both chemical vapor generation and headspace sampling, and a miniature point discharge optical emission spectrometer (µPD-OES) was developed for simultaneous and high-throughput detection of DIC and DOC in seawater and lake water. Phosphoric acid and persulfate were successively injected into sample solutions to convert DIC and DOC to CO2 under the conditions of magnetic stirring and UV irradiation, respectively. Subsequently, the generated CO2 was swept into the µPD-OES for quantitation of DIC and DOC via monitoring carbon atomic emission at 193.0 nm. Under optimal conditions, limits of detection for DIC and DOC (as C) were both 0.01 mg L-1 with relative standard deviations (n = 20) better than 5% and sample throughput of 80 samples per hour. Compared to conventional analyzers, the proposed instrument provides the advantages of high throughput, compactness, low energy consumption and eliminates expensive instruments. The accuracy of the system was validated by simultaneous determination of DIC and DOC in various water samples in laboratory and field environments.

Characteristics and aetiology of low-temperature burns in Beijing of China.

This study was designed to analyse the characteristics and aetiology of low-temperature burns and explore the prevention and treatment strategies. In total, 206 patients hospitalised with low-temperature burns in a major burn center in Beijing from 2017 to 2021 were included. There were 35-49 cases per year, with an average of 41 ± 4.5 cases. The prevalence of low-temperature burns was higher in female than in male and are mainly resulted from two kinds of incidents: unintended burns from heat treatment (50.97%, 105/206) and improper use of heating devices to keep warm (43.69%, 90/206). Most cases occurred in autumn (33.01%, 68/206) and the least in spring (17.96%, 37/206); cases in summer (24.27%, 50/206) and winter (24.76%, 51/206) accounted for nearly a quadrant respectively. Low-temperature burns in summer were mainly unintended burns from heat treatment (80%, 40/50), whereas in autumn were mainly resulted from improper use of heating devices to keep warm (55.88%, 38/68), the difference was statistically significant (χ2  = 42.801, P < .001). Of all the cases, the burn size ranged from 0.2% to 5% TBSA, mostly less than 1% (85.92%, 177/206); third-degree burns accounted for 98.54% (203/206). Patients admitted after 3 weeks post-injury accounted for 42.23% (87/206). All patients were cured, and most of them were by surgeries (70.87%, 146/206). The results of the study show that low-temperature burn injury features a predictable morbidity among different seasons, a higher prevalence in adult women and a frequent occurrence at home. The wounds of low-temperature burns are often small in size but deep in depth, and can be easily misdiagnosed as superficial burns. However, most low-temperature burn wounds require surgical treatment. The study also suggests that based on the characteristics and aetiology of low-temperature burns, targeted prevention and treatment measures should be mapped out.

Accumulation, transportation, and distribution of tetracycline and cadmium in rice.

Co-exposure to heavy metal and antibiotic pollution might result in complexation and synergistic interactions, affecting rice growth and further exacerbating pollutant enrichment. Therefore, our study sought to clarify the influence of different Tetracycline (TC) and Cadmium(Cd) concentration ratios (both alone and combined) on rice growth, pollutant accumulation, and transportation during the tillering stage in hydroponic system. Surprisingly, our findings indicated that the interaction between TC and Cd could alleviate the toxic effects of TC/Cd on aerial rice structures and decrease pollutant burdens during root elongation. In contrast, TC and Cd synergistically promoted the accumulation of TC/Cd in rice roots. However, their interaction increased the accumulation of TC in roots while decreasing the accumulation of Cd when the toxicant doses increased. The strong affinity of rice to Cd promoted its upward transport from the roots, whereas the toxic effects of TC reduced TC transport. Therefore, the combined toxicity of the two pollutants inhibited their upward transport. Additionally, a low concentration of TC promoted the accumulation of Cd in rice mainly in the root tip. Furthermore, a certain dose of TC promoted the upward migration of Cd from the root tip. Laser ablation-inductively coupled plasma mass spectrometry demonstrated that Cd mainly accumulated in the epidermis and stele of the root, whereas Fe mainly accumulated in the epidermis, which inhibited the absorption and accumulation of Cd by the rice roots through the generation of a Fe plaque. Our findings thus provide insights into the effects of TC and Cd co-exposure on rice growth.

Sensitive and Environmentally Friendly Field Analysis of Waterborne Arsenic by Electrochemical Hydride Generation Microplasma Optical Emission Spectrometry.

To avoid polluting the environment, it is desirable to develop methods consuming as few chemicals as possible for field elemental analysis. In this work, a lithium-ion battery supplied, compact handheld optical emission spectrometer (OES) (0.3 kg, length 18 cm × width 5 cm × height 10 cm) was fabricated for the sensitive field analysis of waterborne arsenic by utilizing electrochemical hydride generation (ECHG) and miniaturized ballpoint discharge (µPD) as sample introduction means and excitation source, respectively. The high ECHG efficiency of arsenic was obtained using a superior cathode of Fe@PbO/Pb and the generated arsine was separated from an aqueous phase and further swept to the µPD microplasma for detection. It is worth noting that the Fe@PbO/Pb cathode not only retains advantages of large specific surface area, robust stability, and excellent reproducibility for the ECHG of arsenic but also accomplishes the preconcentration of As(III), thus improving the kinetics of the surface chemistry at the cathode, alleviating the corrosion of the electrode, and minimizing the release of Pb. A limit of detection of 1.0 µg L-1 was obtained with a relative standard deviation of 4.2% for 20 µg L-1 As(III). Owing to the advantages of ECHG and µPD-OES, the system retains a promising potential for the sensitive, cost-effective, and environmentally friendly field analysis of waterborne arsenic.

Effect of the R126C mutation on the structure and function of the glucose transporter GLUT1: A molecular dynamics simulation study.

Glucose transporter 1 (GLUT1) is responsible for basal glucose uptake and is expressed in most tissues under normal conditions. GLUT1 mutations can cause early-onset absence epilepsy and myoclonus dystonia syndrome (MDS), with MDS potentially lethal. In this study, the effect of the R126C mutation, which is associated with MDS, on structural stability and substrate transport of GLUT1 was investigated. Various bioinformatics tools were used to predict the stability of GLUT1, revealing that the R126C mutation reduces the structural stability of GLUT1. Molecular dynamics (MD) simulations were used to further characterize the effect of the R126C mutation on GLUT1 structural stability. Based on the MD simulations, specific conformational changes and dominant motions of the GLUT1 mutant were characterized by Principal component analysis (PCA). The mutation disrupts hydrogen bonds between substrate-binding residues and glucose, thus likely reducing substrate affinity. The R126C mutation reduces the conformational stability of the protein, and fewer intramolecular hydrogen bonds were present in the mutated GLUT1 when compared with that of wild-type GLUT1. The mutation increased the free energy of glucose transport through GLUT1 significantly, especially at the mutation site, indicating that passage of glucose through the channel is hindered, and this mutant may even release cytoplasmic glucose. This study provides a detailed atomic-level explanation for the reduced structural stability and substrate transport capacity of a GLUT1 mutant. The results aid our understanding of the structure of GLUT1 and provide a framework for developing drugs to treat GLUT1-related diseases, such as MDS.

Tetracycline-Induced Release and Oxidation of As(III) Coupled with Concomitant Ferrihydrite Transformation.

Cocontamination with tetracycline (TC) and arsenic (As) is very common in paddy fields. However, the process and underlying mechanism of arsenite (As(III)) transformation on iron mineral surfaces in the presence of antibiotic contaminants remain unclear. In this study, the release and oxidation of As(III) on ferrihydrite (Fh) surfaces and Fh transformation in the presence of TC under both aerobic and anaerobic conditions were investigated. Our results indicated that the TC-induced reductive dissolution of Fh (Fe(II) release) and TC competitive adsorption significantly promote the release of As, especially under anaerobic conditions. The release of As was increased with increasing TC concentration, whereas it decreased with increasing pH. Interestingly, under both aerobic and anaerobic conditions, the addition of TC enhanced the oxidation of As(III) by Fh and induced the partial transformation of Fh to lepidocrocite. Under aerobic conditions, the adsorbed Fe(II) activated the production of reactive oxygen species (·OH and 1O2) from dissolved O2, with Fe(IV) being responsible for As(III) oxidation. Under anaerobic conditions, the abundant oxygen vacancies of Fh affected the oxidation of As(III) during Fh recrystallization. Thus, this study provided new insights into the role of TC on the migration and transformation of As coupled with Fe in soils.

3D printed miniature atomic emission detector coupling with gas chromatography: A sensitive and cost-effective strategy for the determination of volatile methylsiloxanes in municipal sewage.

The determination of volatile methylsiloxanes (VMSs) in municipal sewage has attracted great attention. Gas chromatography-mass spectrometry (GC-MS) is the most mature detection technique for VMSs, however, its instrumentation and operation cost are unfavorable in low- and middle-income countries. Herein, a novel and cost-effective strategy by using a 3D printed miniature microplasma optical emission detector (µAED) as an alternative to MS detector, was developed to detect VMSs in municipal sewage by GC after preconcentration by a laboratory-built automatic purge and trap (P&T) system. Two types of µAEDs have been fabricated and their analytical performances were compared. The one using two tungsten rods as electrodes shows better performance and was thus selected as the detecting system for real sample analysis. Under the optimized conditions, the P&T-GC-µAED system provided limits of detection of 3.6 ng L-1 to 15.5 ng L-1 of Si for tested VMSs. Relative standard deviations were better than 3.0% and good recoveries ranging from 82.4% to 102.8% were obtained for all analytes. The applicability of this system was demonstrated via the measurement of VMSs in the influents and effluents from 10 wastewater treatment plants (WWTPs) in Chengdu, China.

Mechanistic insights into the environmental fate of tetracycline affected by ferrihydrite: Adsorption versus degradation.

Tetracycline (TC), a widely used antibiotic, is frequently detected in soil environments. It has a strong tendency to form complexes with metals, including iron (oxyhydr)oxide. In this study, ferrihydrite (Fh), a representative iron oxyhydroxide of the iron plaques on the surface of plant roots, was chosen to study the contributions of iron oxyhydroxide on the environmental fate of TC in the rhizosphere environment. Fh adsorption isotherm of TC showed good fitting to the Freundlich model, and the Fh adsorption capacity of TC was found much larger than the other iron oxyhydroxide of high crystallinity. The adsorption mechanisms mainly included electrostatic interaction, H-bonding, and complexation. The results of FTIR and XPS spectra revealed that tricarbonylamide, dimethylamino, and the hydroxyl in the B ring of TC were mainly responsible for the complexation with Fh surface hydroxyl groups. Furthermore, it should be noted that the adsorbed TC on Fh could be degraded and the degradation kinetics of TC better fitted to the pseudo-second-order model. Fh could promote electron transfer from TC to Fe(III) on the Fh surface, which led to the degradation of TC and the formation of Fe(II) ions. The degradation pathways of TC mainly involved three reactions: hydroxylation, dealkylation, and deamination. This study provides mechanistic insights on TC-Fh interaction, which improves the understanding of TC fate in the rhizosphere environment.

Three-Dimensional Printed Dual-Mode Chemical Vapor Generation Point Discharge Optical Emission Spectrometer for Field Speciation Analyses of Mercury and Inorganic Selenium.

Due to the large size and high energy consumption of instruments, field elemental speciation analysis is still challenging so far. In this work, a portable and compact system device (230 mm length × 38 mm width × 84 mm height) was fabricated by using three-dimensional (3D) printing technology for the field speciation analyses of mercury and inorganic selenium. The device comprises a cold vapor generator, photochemical vapor generator, and miniaturized point discharge optical emission spectrometer (µPD-OES). For mercury, inorganic mercury (IHg) was selectively reduced to Hg0 by cold vapor generation, whereas the reductions of both IHg and methylmercury (MeHg) were obtained by photochemical vapor generation (PVG) in the presence of formic acid. For selenium, Se(IV) and total inorganic selenium were converted to their volatile species by PVG in the presence and the absence of nano-TiO2, respectively. The generated volatile species were consequently detected by µPD-OES. Limits of detection of MeHg, IHg, Se(IV), and Se(VI) were 0.1, 0.1, 5.2, and 3.5 µg L-1, respectively. Precision expressed as the relative standard deviations (n = 11) were better than 4.5%. The accuracy and practicality of the proposed method were evaluated by the analyses of Certified Reference Materials (DORM-4, DOLT-5, and GBW(E)080395) and several environmental water samples with satisfactory recoveries (95-103%). This work confirms that 3D printing has great potential to fabricate a simple, miniaturized, easy-to-operate, and low gas and power consuming atomic spectrometer for field elemental speciation analysis.

Microplasma-induced vapor generation for rapid, sample preparation-free screening of mercury in fruits and vegetables.

A method for the rapid screening of toxic elements in fruits and vegetables is of significant importance to prevent human exposure to these elements. In this work, a simple method used for microplasma-induced vapor generation (µPIVG) was developed for the rapid screening and quantification of mercury in fruits and vegetables without sample preparation. A stainless-steel capillary was partly inserted into a juice droplet from the tested fruits and then the sample liquid automatically moved to the end of the capillary with the assistance of inherent capillary driving force. Subsequently, a high voltage was applied between the capillary and a tungsten electrode to generate microplasma wherein the juice was sprayed and the mercury ions contained in the juice were converted to mercury cold vapor (Hg0). The Hg0 was finally separated from the liquid phase and swept to an atomic fluorescence spectrometer (AFS) for rapid screening. A standard addition method coupled with µPIVG atomic fluorescence spectrometry was further used for the quantitative analysis of the suspected sample. Under the optimized conditions, the limits of detection (LODs) of 0.3, 0.5, and 0.4 µg L-1 were obtained for the tested tomato, lemon, and orange samples, respectively. The proposed technique provides a simple and cost-effective tool for the rapid screening of mercury in fruits and vegetables by atomic spectrometry.

Methanol-Enhanced Liquid Electrode Discharge Microplasma-Induced Vapor Generation of Hg, Cd, and Zn: The Possible Mechanism and Its Application.

Despite increased interest in microplasma-induced vapor generation (µPIVG) over the past several years, applications in real sample analyses remain limited due to their relatively low vapor generation efficiency and ambiguous mechanism. In this work, a novel method using methanol for significantly enhancing the liquid electrode discharge µPIVG efficiency was developed for the simultaneous and sensitive determination of Hg, Cd, and Zn by atomic fluorescence spectrometry (AFS). It is worth noting that the possible enhancement mechanism was investigated via the characterizations of volatile products by AFS, microplasma optical emission spectrometry, online gas chromatography, and gas chromatography-mass spectrometry, which involved the reductive species such as electrons, hydrogen radicals (·H), methyl radicals (·CH3), and other intermediates in the argon plasma adding methanol. Under the optimized conditions, the limits of detection of 0.007, 0.05, and 0.5 µg L-1 were obtained for Hg, Cd, and Zn, respectively, with relative standard deviations of 3.1, 3.7, and 5.2% for these elements, respectively. Vapor generation efficiencies of 90, 83, and 55% were achieved for Hg, Cd, and Zn, respectively, and improved 2.7-, 4.8-, and 7.9-fold, respectively, compared to those obtained in the absence of methanol. The accuracy and practicability of the proposed method were validated by the determination of Hg, Cd, and Zn in a certified reference material (CRM, Lobster hepatopancreas, TORT-3) and crayfish samples collected from three different provinces of China.