RESUMEN
6-Thioguanine encapsulated chitosan nanoparticles (6-TG-CNPs) has formulated by the ionic-gelation method. Morphologically, the 6-TG-CNPs were spherical and showed mean size, PDI, zeta potential, and entrapment efficiency of 261.63 ± 6.01 nm, 0.34 ± 0.10, +15.97 ± 0.46 mV and 44.27%, respectively. The IR spectra confirmed the 6-TG complex with chitosan. The in vitro drug release profile of 6-TG-CNPs revealed an increase in sustained-release (91.40 ± 1.08% at 48 h) at pH 4.8 compared to less sustained-release (73.96 ± 1.12% at 48 h) at pH 7.4. The MTT assay was conducted on MCF-7 and PA-1 cell lines at 48 h incubation to determine % cell viability. The IC50 values of 6-TG, 6-TG-CNPs, and curcumin for MCF-7 were 23.09, 17.82, and 15.73 µM, respectively. Likewise, IC50 values of 6-TG, 6-TG-CNPs, and curcumin for PA-1 were 5.81, 3.92, and 12.89 µM, respectively. A combination of 6-TG-CNPs (IC25) with curcumin (IC25) on PA-1 and MCF-7 showed % cell viability of 43.67 ± 0.02 and 49.77 ± 0.05, respectively. The in vitro cytotoxicity potential in terms of % cell viability, early apoptosis, G2/M phase arrest, and DNA demethylating activity of 6-TG-CNPs alone and combination with curcumin proved to be more effective than that of 6-TG on PA-1 cells.
Asunto(s)
Antineoplásicos/farmacología , Quitosano/química , Curcumina/química , Nanopartículas/química , Tioguanina/farmacología , Antineoplásicos/química , Apoptosis/efectos de los fármacos , Puntos de Control del Ciclo Celular/efectos de los fármacos , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Portadores de Fármacos/química , Liberación de Fármacos , Humanos , Células MCF-7 , Tamaño de la Partícula , Tioguanina/químicaRESUMEN
A five year old Holstein-Friesian cross breed cow was brought to Teaching Veterinary Clinical Complex with the history of anorexia, shivering and respiratory distress since a week. On close physical examination, enlarged prescapular lymph nodes and conjunctival mucous membranes with petechiae were noticed. Physiological parameters like rectal temperature, heart rate and respiratory rates were found to be 105.3 °F, 86 beats per minute and 48 per minute respectively. On hematological examination, hemoglobin and total erythrocyte count were found to be low i.e., 5.2 g/dl and 3.2 million cells/cmm respectively. Peripheral blood smear examination revealed the presence of Anaplasma bovis organisms in the monocytes. Based on these findings a diagnosis of bovine monocytic anaplasmosis was made and the case was treated with two doses of long acting Oxytetracycline @20 mg/kg body weight 48 h apart, I/M, and Meloxicam @0.5 mg/kg body weight, I/M for 5 days. Improvement was noticed after 3 days of treatment.
