RESUMEN
Tumor-associated eosinophilia has been observed in numerous human cancers and several tumor models in animals; however, the details surrounding this eosinophilia remain largely undefined and anecdotal. We used a B16-F10 melanoma cell injection model to demonstrate that eosinophil infiltration of tumors occurred from the earliest palpable stages with significant accumulations only in the necrotic and capsule regions. Furthermore, the presence of diffuse extracellular matrix staining for eosinophil major basic protein was restricted to the necrotic areas of tumors, indicating that eosinophil degranulation was limited to this region. Antibody-mediated depletion of CD4+ T cells and adoptive transfer of eosinophils suggested, respectively, that the accumulation of eosinophils is not associated with T helper cell type 2-dependent immune responses and that recruitment is a dynamic, ongoing process, occurring throughout tumor growth. Ex vivo migration studies have identified what appears to be a novel chemotactic factor(s) released by stressed/dying melanoma cells, suggesting that the accumulation of eosinophils in tumors occurs, in part, through a unique mechanism dependent on a signal(s) released from areas of necrosis. Collectively, these studies demonstrate that the infiltration of tumors by eosinophils is an early and persistent response that is spatial-restricted. It is more important that these data also show that the mechanism(s) that elicit this host response occur, independent of immune surveillance, suggesting that eosinophils are part of an early inflammatory reaction at the site of tumorigenesis.
Asunto(s)
Quimiotaxis/fisiología , Eosinófilos/inmunología , Inflamación/inmunología , Melanoma Experimental/inmunología , Animales , Antígenos CD4/inmunología , Linfocitos T CD4-Positivos/inmunología , Células Cultivadas , Factores Quimiotácticos/metabolismo , Quimiotaxis/efectos de los fármacos , Medios de Cultivo Condicionados/química , Medios de Cultivo Condicionados/farmacología , Eosinofilia/etiología , Eosinofilia/fisiopatología , Eosinófilos/trasplante , Vigilancia Inmunológica , Inmunoterapia Adoptiva , Inflamación/patología , Inyecciones Subcutáneas , Interleucina-5/genética , Depleción Linfocítica , Melanoma Experimental/complicaciones , Melanoma Experimental/metabolismo , Melanoma Experimental/patología , Melanoma Experimental/terapia , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Microscopía Confocal , Necrosis , Trasplante de Neoplasias , Células Th2/inmunologíaRESUMEN
BACKGROUND: Severe respiratory syncytial virus infection (RSV) during infancy has been shown to be a major risk factor for the development of subsequent wheeze. However, the reasons for this link remain unclear. The objective of this research was to determine the consequences of early exposure to RSV and allergen in the development of subsequent airway hyperreactivity (AHR) using a developmental time point in the mouse that parallels that of the human neonate. METHODS: Weanling mice were sensitized and challenged with ovalbumin (Ova) and/or infected with RSV. Eight days after the last allergen challenge, various pathophysiological endpoints were examined. RESULTS: AHR in response to methacholine was enhanced only in weanling mice exposed to Ova and subsequently infected with RSV. The increase in AHR appeared to be unrelated to pulmonary RSV titer. Total bronchoalveolar lavage cellularity in these mice increased approximately two-fold relative to Ova alone and was attributable to increases in eosinophil and lymphocyte numbers. Enhanced pulmonary pathologies including persistent mucus production and subepithelial fibrosis were observed. Interestingly, these data correlated with transient increases in TNF-alpha, IFN-gamma, IL-5, and IL-2. CONCLUSION: The observed changes in pulmonary structure may provide an explanation for epidemiological data suggesting that early exposure to allergens and RSV have long-term physiological consequences. Furthermore, the data presented here highlight the importance of preventative strategies against RSV infection of atopic individuals during neonatal development.
Asunto(s)
Hipersensibilidad Respiratoria/diagnóstico , Hipersensibilidad Respiratoria/virología , Infecciones por Virus Sincitial Respiratorio/diagnóstico , Infecciones por Virus Sincitial Respiratorio/virología , Virus Sincitiales Respiratorios/aislamiento & purificación , Animales , Pruebas de Provocación Bronquial , Cloruro de Metacolina , Ratones , Ratones Endogámicos BALB C , Ovalbúmina , Hipersensibilidad Respiratoria/inducido químicamente , Hipersensibilidad Respiratoria/complicaciones , Hipersensibilidad Respiratoria/fisiopatología , Infecciones por Virus Sincitial Respiratorio/complicaciones , Infecciones por Virus Sincitial Respiratorio/fisiopatologíaRESUMEN
Eosinophils are often dominant inflammatory cells present in the lungs of asthma patients. Nonetheless, the role of these leukocytes remains poorly understood. We have created a transgenic line of mice (PHIL) that are specifically devoid of eosinophils, but otherwise have a full complement of hematopoietically derived cells. Allergen challenge of PHIL mice demonstrated that eosinophils were required for pulmonary mucus accumulation and the airway hyperresponsiveness associated with asthma. The development of an eosinophil-less mouse now permits an unambiguous assessment of a number of human diseases that have been linked to this granulocyte, including allergic diseases, parasite infections, and tumorigenesis.
Asunto(s)
Asma/patología , Asma/fisiopatología , Eosinófilos/fisiología , Pulmón/patología , Pulmón/fisiopatología , Alérgenos/inmunología , Animales , Asma/inmunología , Toxina Diftérica/genética , Peroxidasa del Eosinófilo , Marcación de Gen , Recuento de Leucocitos , Pulmón/inmunología , Ratones , Ratones Transgénicos , Modelos Animales , Moco/metabolismo , Ovalbúmina/inmunología , Fragmentos de Péptidos/genética , Peroxidasas/genética , Hipersensibilidad Respiratoria/inmunología , Hipersensibilidad Respiratoria/patologíaRESUMEN
Adenosine signaling has been implicated in chronic lung diseases such as asthma and chronic obstructive pulmonary disease; however, the specific roles of the various adenosine receptors in processes central to these disorders are not well understood. In this study, we have investigated the role(s) of the A(3) adenosine receptor in adenosine-dependent pulmonary inflammation observed in adenosine deaminase (ADA)-deficient mice. The A(3) receptor (A(3)R) was found to be expressed in eosinophils and mucus-producing cells in the airways of ADA-deficient mice. Treatment of ADA-deficient mice with MRS 1523, a selective A(3)R antagonist, prevented airway eosinophilia and mucus production. Similar findings were seen in the lungs of ADA/A(3) double knockout mice. Although eosinophils were decreased in the airways of ADA-deficient mice following antagonism or removal of the A(3)R, elevations in circulating and lung interstitial eosinophils persisted, suggesting signaling through the A(3)R is needed for the migration of eosinophils into the airways. These findings identify an important role for the A(3)R in regulating lung eosinophilia and mucus production in an environment of elevated adenosine.
Asunto(s)
Adenosina Desaminasa/deficiencia , Moco/metabolismo , Receptor de Adenosina A3/inmunología , Sistema Respiratorio/inmunología , Transducción de Señal/inmunología , Adenosina Desaminasa/genética , Animales , Eosinófilos/metabolismo , Inflamación/inmunología , Ratones , Ratones Noqueados , ARN Mensajero/metabolismo , Receptor de Adenosina A3/genética , Sistema Respiratorio/metabolismoRESUMEN
The eosinophil-associated ribonuclease (Ear) family in the mouse consists of thirteen genes, eleven of which encode RNases that have physical/functional properties similar to the human Ears, eosinophil-derived neurotoxin and eosinophil cationic protein. The expression of Ear genes in the mouse is confined to sites of known eosinophilopoiesis, with the exception of the lung. Two Ear genes, Ear1 and Ear2, are predominantly expressed in the lungs of naive mice. Total Ear gene expression and RNase activity in bronchoalveolar lavage fluid increases significantly upon the induction of pulmonary inflammation using an ovalbumin (OVA) model of allergic sensitization and challenge. Interestingly, pulmonary Ear11 transcripts, which are absent in naive mice, accumulate as a consequence of OVA-mediated T(H)2 inflammation in the lung. The induction of Ear11 expression is dependent on the presence of T cells, in particular, CD4(+) T lymphocytes. This effect is likely the result of the elaboration of T(H)2 cytokine levels, because pulmonary instillation of interleukin-4 or interleukin-13 induces the accumulation of Ear11 transcripts in naive animals. This study demonstrates that despite an allergen-mediated pulmonary eosinophilia and earlier studies showing that Ears are constituents of eosinophil secondary granules, alveolar macrophages are a significant source of these RNases in lungs of OVA-treated mice.