Pan-cancer analyses reveal genomics and clinical outcome association of the fatty acid oxidation regulators in cancer.

Background: Fatty acid oxidation (FAO) is considered to play a vital part in tumor metabolic reprogramming. But the comprehensive description of FAO dysregulation in tumors has not been unknown. Methods: We obtained FAO genes, RNA-seq data and clinical information from the Msigdb, TCGA and GTEx databases. We assessed their prognosis value using univariate cox analysis, survival analysis and Kaplan-Meier curve. We determined the function of FAO genes using gene set variation analysis. The correlation analysis was calculated by corrplot R package. Immunotherapy response was assessed through TIDE scores. The protein expression levels of FAO genes were validated using immunohistochemistry (IHC). Results: The FAO scores were highest in COAD but lowest in PCPG. FAO scores were significantly associated with the prognosis of some cancers in OS, DSS, DFI and PFI. Besides, gene set variation analysis identified that FAO scores were related to immune-related pathways, and immune infiltration analysis showed FAO scores were positively related to cancer-associated fibroblasts and various immune-related genes. TIDE scores were significantly decreased in ACC, CHOL, ESCA, GBM, LAML, SARC, SKCM and THCA compared with normal samples, while it was significantly increased in BLCA, LUAD, LUSC, PCPG, PRAD and STAD. Besides, most FAO genes were downregulated in pan-cancer compared with normal samples. Moreover, we found copy number variation (CNV) of FAO genes played a positive role in their mRNA expression, while methylation was negative. We determined FAO genes were closely related to some drugs in pan-cancer. Conclusions: FAO score is a novel and promising factor for predicting outcomes.

[Benefit of network education to college students' knowledge about sexual and reproductive health in Ningbo city].

OBJECTIVE: To investigate how network education can improve college students' knowledge on sexual and reproductive health in Ningbo city. METHODS: From December 2012 to June 2013, we conducted a questionnaire investigation among college students in Ningbo city about the effects of network education on their knowledge about sexual psychology, sexual physiology, sexual ethics, and reproductive health. RESULTS: A total of 7 362 college students accomplished the investigation, of whom 2 483 (42.1% males and 57.9% females) received network education, while the other 4 879 (24.1% males and 75.9% females) did not. Approximately 47.1% of the male and 28.0% of the female students acquired sexual and reproductive knowledge via network education. Reproductive health-related network education significantly enriched the students' knowledge about the reproductive system and sex, pubertal development, sexual physiology, conception and embryonic development, methods of contraception, sexual psychology, sexually transmitted diseases and their prevention, pregnancy care and eugenics, and environment- and occupation-related reproductive health (P < 0.01). It also remarkably improved their cognitive attitude towards reproductive health knowledge (P < 0.01). Those who received reproductive health-related network education showed a significantly higher rate of masturbation (P < 0.01) but markedly later time of the first masturbation (P < 0.01) than those who did not. CONCLUSION: Network education can enhance the effect of reproductive health education among college students and improve their sexual experience and health.

[Types of arterio-portal fistula of hepatocellular carcinoma patients on helical computed tomography and digital subtraction angiography].

OBJECTIVE: To analyse the pathogenesis of the hepatocellular carcinoma (HCC) with arterio-portal fistula (APF) and the types of these APF on helical CT (HCT) and digital subtraction angiography (DSA), and to study the interventional therapy for HCC patients with APF. METHODS: HCC with APF in 48 patients were reported. All the patients received contrast media enhanced HCT scan and DSA examination. The APF were divided into the peripheral type, the hepatic segment type, and the center type according to the findings on HCT and DSA. RESULTS: The peripheral type of APF were found in 14 cases (14/48), the hepatic segment type in 15 cases (15/48), and the center type in 19 cases (19/48) on HCT scan, respectively. The peripheral type of APF were found in 13 cases (13/48), the hepatic segment type in 16 cases (16/48), and the center type in 19 cases (19/48) on DSA examination, respectively. The pertinency and consistency on the types of APF between HCT and DSA were compared, and the consistency rate was 85.42%; Kappa=0.779, u=7.60, P<0.01. It showed high pertinency of the types of APF between HCT and DSA. The center type APF in 11 of 19 cases disappeared, in 6 of the 19 cases partly disappeared, in 1 case was noneffective, and in 1 case was reformulation after the interventional therapy. The segment and peripheral types of APF in 20 of the 29 cases disappeared, in 8 of the 29 cases partly disappeared, and 1 case was noneffective after interventional therapy. CONCLUSION: It's vital to know well the features of various types of APF in HCC on HCT and DSA, which can bring about positive effect on interventional therapy for HCC with APF.