P27 Promotes TGF-ß-Mediated Pulmonary Fibrosis via Interacting with MTORC2.

Pulmonary fibrosis (PF), a progressive and life-threatening pulmonary disease, is the main pathological basis of interstitial lung disease (ILD) which includes the idiopathic pulmonary fibrosis (IPF). No effective therapeutic strategy for pulmonary fibrosis has been established. TGF-ß signaling has emerged as the vital regulator of PF; however, the detailed molecular mechanisms of TGF-ß in PF were uncertain. In the present study, we proved that inhibition of MTORC2 suppresses the expression of P27 in MRC5 and HLF cells. And in bleomycin-induced PF model, the expression of α-SMA and P27 was upregulated. Moreover, TGF-ß application increased the level of α-SMA, vimentin, and P27 in MRC5 and HLF cells. Furthermore, P27 overexpression advanced the cell cycle process and promoted the proliferation of MRC5 and HLF cells. Finally, the rescue experiment showed that MTORC2 knockdown reversed P27 overexpression-induced cell cycle acceleration and proliferation. Thus, our results suggest that P27 is involved in TGF-ß-mediated PF, which was regulated by MTORC2, providing a novel insight into the development of PF.

Two Highly Stable Luminescent Lead Phosphonates Based on Mixed Ligands: Highly Selective and Sensitive Sensing for Thymine Molecule and VO3 - Anion.

Two luminescent lead phosphonates with two-dimensional (2D) layer and three-dimensional (3D) framework structure, namely, Pb3[(L1)2(Hssc)(H2O)2] (1) and [Pb2(L2)0.5(bts)(H2O)2]·H2O (2) (H2L1 = O(CH2CH2)2NCH2PO3H2, H4L2 = H2PO3CH2NH(C2H4)2NHCH2PO3H2, H3ssc = 5-sulfosalicylic acid, NaH2bts = 5-sulfoisophthalic acid sodium) have been prepared via hydrothermal techniques. The two compounds not only show excellent thermal stability but also remain intact in aqueous solution within an extensive pH range. Moreover, the atomic absorption spectroscopy analysis experiment indicates that there does not exist the leaching of Pb2+ ions from the lead phosphonates, which show they are nontoxic in aqueous solution. In compound 1, the Pb(1)O4, Pb(2)O7, Pb(3)O4, and CPO3 polyhedra are interlinked into a one-dimensional chain, which is further connected to adjacent chain by sharing the Hssc2- to form a 2D layer. Interestingly, compound 1 as a highly selective and sensitive luminescent material can be used to detect the thymine molecule with a very low detection limit of 8.26 × 10-7 M. In compound 2, the Pb(1)O6 and Pb(2)O5 polyhedra are interlinked into a dimer via edge sharing, which is further connected to adjacent dimer to form a tetramer via corner sharing, and such a tetramer is then interlinked into a 2D layer through bts3- ligands; the adjacent 2D layers are finally constructed to a 3D structure by sharing the L2 4- ligand. Compound 2 can be applied as an excellent luminescent sensor for sensing of VO3 - anion. Furthermore, the probable fluorescent quenching mechanisms of the two compounds have also been studied.

Terbium Oxalatophosphonate as Efficient Multiresponsive Luminescent Sensors for Chromate Anions and Tryptophan Molecules.

A stable 2D terbium oxalatophosphonate with green luminescence, namely, [Tb2(H3L)(C2O4)3(H2O)4]·2H2O (1), has been hydrothermally obtained by using (4-carboxypiperidyl)-N-methylenephosphonic acid (H3L) and oxalate ligand. The luminescent investigation indicates that the emission behavior of compound 1 shows high water and pH stabilities. It can be applied as a multiresponsive luminescent probe with high selectivity, high sensitivity, recycling capability, and fast sensing of CrO4 2-, Cr2O7 2- anions and tryptophan (Trp) molecules in aqueous solution through the luminescence quenching effect. Moreover, the sensing results can be distinguished by the naked eye under the irradiation of UV light of 254 nm. In addition, the probable mechanisms for the quenching behavior are also discussed, which can be mainly attributed to the competitive absorption of excitation energy between compound 1 and the analytes.

Stereotactic body radiation therapy for centrally-located lung tumors.

The application of high-dose irradiation to centrally-located lung tumors is generally considered to be of high risk in causing bronchial injury. The aim of the present retrospective study was to investigate the safety and efficacy of stereotactic body radiation therapy (SBRT) for patients with centrally-located lung tumors. In total, 28 patients who underwent SBRT for lung tumors within 2 cm of a major bronchus were retrospectively analyzed. The median total dose prescribed was 45 Gy (range, 36.3-52.5 Gy), the median fraction was 12 (range, 10-15) and the median dose per fraction was 3.6 Gy (range, 3-5 Gy). The median follow-up period for the surviving patients was 14 months (range, 10-41 months). The local control rate of SBRT was 100%, with a complete response (CR) rate of 32.1% (9/28); a partial response (PR) rate of 50% (14/28) and a stable disease (SD) rate of 17.9% (5/28). In total, 15 patients survived and 13 patients succumbed; 11 patients succumbed to tumor progression, one to congestive heart failure and one to a brain hemorrhage. The main side-effects included grade 2 esophagitis (17.9%; 5/28) atelectasis (10.7%; 3/28) and grade 2 late radiation pneumonitis (7.1%; 2/28). Severe late toxicity (≥ grade 3) was not observed in any patient. SBRT is an effective and safe therapy for centrally-located lung tumors.

Slow magnetic relaxation in a cyano-bridged ferromagnetic {Fe(III)Ni(II)} alternating chain.

A cyano-bridged ferromagnetic {Fe(III)Ni(II)} alternating chain {[Fe(pzTp)(CN)3][Ni(chxn)2]}·ClO4·H2O (1) (pzTp = tetrakis(pyrazolyl)borate, chxn = (1R,2R)-1,2-diaminocyclohexane) was synthesized via rational design. Crystal structure analysis and magnetic studies demonstrated that compound had a one-dimensional zigzagging chain-like structure and slow magnetic relaxation.

A series of novel lanthanide carboxyphosphonates with a 3D framework structure: synthesis, structure, and luminescent and magnetic properties.

By introduction of 1,4-benzenedicarboxylic acid as the second organic ligand, a series of novel lanthanide carboxyphosphonates with a 3D framework structure, namely, [Ln(3)(H(2)L)(HL)(2)(bdc)(2)(H(2)O)]·7H(2)O (Ln = La (1), Ce (2), Pr (3), Nd (4), Sm (5), Eu (6), Gd (7), Tb (8); H(3)L = H(2)O(3)PCH(2)NC(5)H(9)COOH; H(2)bdc = HOOCC(6)H(4)COOH) have been synthesized under hydrothermal conditions. Compounds are isostructural and feature a 3D framework in which Ln(III) polyhedra are interconnected by bridging {CPO(3)} tetrahedra into 2D inorganic layers parallel to the ab plane. The organic groups of H(2)L(-) are grafted on the two sides of the layer. These layers are further cross-linked by the bdc(2-) ligands from one layer to the Ln atoms from the other into a pillared-layered architecture with one-dimensional channel system along the a axis. The thermal stability of compounds has been investigated. Luminescent properties of compounds , and the magnetic properties of compound have also been studied.