RESUMEN
Nanofibrous polymeric materials, combined with protein therapeutics, play a significant role in biomedical and pharmaceutical applications. However, the upload of proteins into nanofibers with a high yield and controlled release has been a challenging issue. Here, we report the in situ loading of a model protein (bovine serum albumin) into hydrophilic poly(vinyl alcohol) nanofibers via ice-templating, with a 100% protein drug loading efficiency. These protein-loaded nanofibers were further coated by polydopamine in order to improve the nanofiber stability and achieve a controlled protein release. The mass ratio between poly(vinyl alcohol) and bovine serum albumin influenced the percentage of proteins in composite nanofibers and fiber morphology. More particles and less nanofibers were formed with an increasing percentage of bovine serum albumin. By varying the coating conditions, it was possible to produce a uniform polydopamine coating with tunable thickness, which acted as an additional barrier to reduce burst release and achieve a more sustained release profile.
RESUMEN
High sulfur content polymers prepared by inverse vulcanization have many reported potential applications, including as novel antimicrobial materials. High sulfur content polymers usually have limited water-solubility and dispersibility due to their hydrophobic nature, which could limit the development of their applications. Herein, we report the formulation of high sulfur content polymeric nanoparticles by a nanoprecipitation and emulsion-based method. High sulfur content polymeric nanoparticles were found to have an inhibitory effect against important bacterial pathogens, including Gram-positive methicillin-resistant Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa. Salt-stable particles were formulated with the addition of a surfactant, which did not inhibit the antibacterial activity of the polymeric particles. Furthermore, the polymeric nanoparticles were found to inhibit S. aureus biofilm formation and exhibited low cytotoxicity against mammalian liver cells. Interaction of the polymeric particles with cellular thiols could be a potential mechanism of action against bacterial cells, as demonstrated by reaction with cysteine as a model thiol. The findings presented demonstrate methods of preparing aqueous dispersions of high sulfur content polymeric nanoparticles that could have useful biological applications.
Asunto(s)
Staphylococcus aureus Resistente a Meticilina , Nanopartículas , Animales , Staphylococcus aureus , Polímeros/farmacología , Polímeros/química , Antibacterianos/farmacología , Antibacterianos/química , Azufre/química , Nanopartículas/química , Pruebas de Sensibilidad Microbiana , MamíferosRESUMEN
Elemental sulfur (S8), a by-product of the petroleum refining industries, possesses many favourable properties including photocatalytic activity and antibacterial activity, in addition to being intrinsically hydrophobic. Despite this, there is a relative lack of research employing elemental sulfur and/or sulfur copolymers within superhydrophobic materials design. In this work, we present the use of sulfur copolymers to produce superhydrophobic materials with advanced functionalities. Using inverse vulcanization and the use of a natural organic crosslinker, perillyl alcohol (PER), stable S8-PER copolymers were synthesised and later combined with silica (SiO2) nanoparticles, to achieve highly water repellent composites that displayed both antimicrobial and photocatalytic properties, in the absence of carcinogenic and/or expensive materials. Here, we investigated the antibacterial performance of coatings against the Staphylococcus aureus bacterial strain, where coatings displayed great promise for use in antifouling applications, as they were found to limit surface adhesion by more than 99%, when compared to uncoated glass samples. Furthermore, UV dye degradation tests were performed, utilizing the commercially available dye resazurin, and it was shown that coatings had the potential to simultaneously exhibit surface hydrophobicity and photoactivity, demonstrating a great advancement in the field of superhydrophobic materials.
Asunto(s)
Antiinfecciosos , Polímeros , Antibacterianos/farmacología , Polímeros/química , Polímeros/farmacología , Dióxido de Silicio/química , Azufre/química , Agua/químicaRESUMEN
Inverse vulcanization is a bulk polymerization method for synthesizing high sulfur content polymers from elemental sulfur, a byproduct of the petrochemical industry, with vinylic comonomers. There is growing interest in polysulfides as novel antimicrobial agents due to the antimicrobial activity of natural polysulfides found in garlic and onions (Tsao et al. J. Antimicrob. Chemother. 2001, 47, 665-670). Herein, we report the antibacterial properties of several inverse vulcanized polymers against Gram-positive Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa, two common causes of nosocomial infection and pathogens identified by the World Health Organization as priorities for antimicrobial development. High sulfur content polymers were synthesized with different divinyl comonomers and at different sulfur/comonomer ratios, to determine the effect of such variables on the antibacterial properties of the resulting materials. Furthermore, polymers were tested for their potential as antibacterial materials at different temperatures. It was found that the test temperature influenced the antibacterial efficacy of the polymers and could be related to the glass transition temperature of the polymer. These findings provide further understanding of the antibacterial properties of inverse vulcanized polymers and show that such polymers have the potential to be used as antibacterial surfaces.