Study on the Effect of Molecular Weight on the Gut Microbiota Fermentation Properties of Blackberry Polysaccharides In Vitro.

This study aimed to investigate the effect of different molecular weights on the metabolic characteristics of blackberry polysaccharides (BBP). After degradation, three fractions, namely, BBP-8, BBP-16, and BBP-24, were obtained. During fermentation, all polysaccharide fractions were significantly degraded and utilized by the intestinal microbiota, and the lower-molecular-weight polysaccharides were easier to be fermented with higher gas production and carbohydrate consumption rates. Furthermore, the monosaccharide utilization sequence of all polysaccharides was glucose > galactose > arabinose > galacturonic acid. In addition, the lower-molecular-weight polysaccharides had a faster short-chain fatty acid (SCFA) production rate but did not affect the final SCFA yields. The fermentation of BBP promoted the increase of Bacteroidetes and the decrease of Firmicutes. The proportions of Bacteroidetes in BBP, BBP-8, BBP-16, and BBP-24 were 45.41, 47.50, 48.08, and 50.09%, respectively.

A dynamic view on the chemical composition and bioactive properties of mulberry fruit using an in vitro digestion and fermentation model.

Mulberry is a kind of fruit rich in nutrients, however, the beneficial effects of mulberry fruits are related not only to the amount consumed, but also to the bioavailability of these nutrients in the organism. Hence, the aim of this study was to evaluate the bioaccessibility of main bioactive compounds from mulberry fruit using an in vitro digestion model, the changes in bioactivities as well as intestinal flora were also investigated. The results showed that the particle size of the mulberry fruit was gradually reduced (from 196.87 to 60.85 µm), as well as the phenolics and carbohydrates were significantly released during the digestion and maximized in the first 15 min in the intestinal phase (1752 ± 2.80 mg GAE per 100 g, DW; 277.402 ± 2.80 mg GE per 100 g, DW, respectively). Meanwhile, the bioaccessibility indices for phenolic compounds and carbohydrates were 55.49% and 84.62%. The antioxidant activity and α-glucosidase inhibitory effect of the mulberry fruit were positively correlated with their total content of released phenolic compounds. And the phenolic compounds (2,4,6-trihydroxybenzoic acid, cyanidin-3-O-glucoside, 3,4-dihydroxybenzoic acid and gallic acid) were the main compounds that inhibit the α-glucosidase activity by binding to its active cavity through hydrogen bonds. In addition, the mulberry fruit undigested fractions could be further fermented by intestinal microorganisms to produce short-chain fatty acids (SCFAs), which decreased the colon pH value (from 5.93 to 4.79) and the Firmicutes/Bacteroidetes ratio which was beneficial for obesity. Our results indicated that the mulberry fruit exhibited good bioactivity during digestion and fermentation, and could be a promising candidate as a dietary source of functional foods.

The structure, conformation, and hypoglycemic activity of a novel heteropolysaccharide from the blackberry fruit.

A novel heteropolysaccharide fraction (BBP-24-3) with a relative molecular weight of 145.1 kDa was isolated from blackberry fruits. The BBP-24-3 was mainly composed of arabinose, glucose, and galacturonic acid with a ratio of 5.30 : 3.60 : 91.10 mol%. Structural analysis showed that BBP-24-3 possessed a 1,6-linked ß-d-Glcp, 1, 2, 3, 5 linked α-l-Araf, and 1, 4 linked α-d-GalpA backbone with branches substituted at the C-2 and C-5 positions of arabinose units, which included 1, 2, 3, 4 linked ß-d-Glcp and T-linked ß-d-GalpA. The conformation analysis indicated that BBP-24-3 exhibited a solid spherical structure with a uniform distribution in 0.1 M NaCl solution. The BBP-24-3 exhibited excellent α-glucosidase inhibitory activity with an IC50 value of 3.70 mg mL-1, which was due to the structural change, including α-helix and random coil of α-glucosidase caused by BBP-24-3. The current work suggests the potential utilization of BBP-24-3 as an α-glucosidase inhibitor in healthy food for reducing the postprandial blood glucose level.

Comparative study on the effect of extraction solvent on the physicochemical properties and bioactivity of blackberry fruit polysaccharides.

In this study, hot water, 0.1 M HCl and 0.1 M NaOH and 0.1 M NaCl solution were separately used for extraction of blackberry polysaccharides (BPs: Hw, Ac, Al and Na). The physicochemical properties and biological activities were then investigated and compared. Results showed that the extraction yield, molecular weight, monosaccharide composition, particle size, triple-helical structure, surface morphology and rheological properties of BPs were greatly affected by extraction solvents. Bioactivity assays implied that the four BPs showed that the polysaccharides (Hw and Na) with higher molecular weight had stronger antioxidant and α-glucosidase inhibitory activity. Moreover, anti-glycated assay indicated that BPs with higher molecular weight and higher content of galacturonic acid possessed better inhibition of AGEs formation. These results suggested that the higher molecular weight of blackberry polysaccharide could be developed as a beneficial bioactive ingredient for diabetes mellitus and complications.

Antimicrobial effect of black pepper petroleum ether extract for the morphology of Listeria monocytogenes and Salmonella typhimurium.

This study aimed to evaluate the effects of black pepper petroleum extract (BPPE) on pathogenic bacteria. The extraction from black pepper showed intense antimicrobial activity against the Gram-positive Listeria monocytogenes ATCC 19115 and the Gram-negative bacteria Salmonella typhimurium ATCC 14028. The minimum inhibitory concentrations of BPPE against L. monocytogenes and S. typhimurium were 0.625 and 1.25 mg/ml, respectively. Detection of Alkaline phosphatase outside the cell revealed that BPPE treatment destroyed the cell wall integrity. BPPE also altered the membrane integrity, thereby causing leaching of 260 and 280 nm UV-absorbing materials into the medium, particularly, nucleic acids and proteins. Propidium iodide infiltration experiments also indicated that BPPE treatment altered the permeability of bacterial cell membrane. Moreover, Na+/K+-ATPase activity was inhibited by BPPE. And the results of scanning electron microscopy showed that BPPE treatment damaged the morphology of the tested bacteria. These results indicated that BPPE could destroy cell wall integrity, alter the permeability of cell membrane, and inhibit the activity of intracellular enzyme, which could kill bacteria.