Polyphyllin VII as a potential medication for targeting epithelial mesenchymal transitionin in thyroid cancer.

The need for novel anti-thyroid cancer (TC) medications is urgent due to the rising incidence and metastatic rates of malignant TC. In this study, we investigated the effect of Polyphyllin VII (PPVII) to TC cells, and explored their potential mechanism. B-CPAP and TPC-1 cells, were used to analyze the antitumor activity of PPVII by quantifying cell growth and metastasis as well as to study the effect on epithelial mesenchymal transition (EMT). The results showed that PPVII dramatically reduced the capacity of B-CPAP and TPC-1 cells to proliferate and migrate in a dose-response manner. Following PPVII treatment of TC cells, the expression levels of E-cadherin progressively increased and were higher than the control group, while the expression levels of EMT-related genes Vimentin, N-cadherin, Slug, Zeb-1, and Foxe1 gradually declined and were lower than the control group. It was proposed that PPVII might prevent TC from undergoing EMT. The Foxe1 gene was shown to be significantly expressed in TC, and a statistically significant variation in Foxe1 expression was observed across clinical stages of the disease, according to a bioinformatics database study. There was a strong link between the expression of the Foxe1 gene and the EMT-related gene. In the meantime, TC cells' expression of Foxe1 can be inhibited by PPVII. In conclusion, our results showed that PPVII may as a potential medication for targeting EMT in thyroid cancer.

A demethylated lignin improved PVA-based supramolecular plastic with tough, degradable, and water-resistant performances.

Poly (vinyl alcohol) (PVA), as an excellent degradable plastic feedstock, is limited by its diminishing stability in wet environment, low strength, thermal instability and nonopaque properties. In response to these concerns, a PVA/demethylated lignin-based supramolecular plastic (DPVA-HA-Fe-5) was designed and produced from PVA, demethylated lignin (DL), humic acid (HA) and Fe3+ ions via a simple casting method. As compared with pure PVA plastic, the tensile strength of DPVA-HA-Fe-5 were increased by 411 % to 410.61 MPa, and the breaking strain was increased by 149 % to 239.47 %. Notably, the hydrophobicity of DPVA-HA-Fe-5 was also significantly improved. Although in highly humid environment (stored in RH = 100 % for 10 days) or in alkaline organic solvent (stored in pyridine for 3 h), DPVA-HA-Fe-5 also showed excellent mechanical strengths of 302.9 and 222.99 MPa, respectively, which are equivalent or even superior to the most of commercial petroleum-based plastics. Moreover, the prepared plastics showed an outstanding UV resistance and shading performance, and about 98.3 % protection against ultraviolet radiation B rays and 90.7 % protection against visible light were obtained. In short, the introduction of lignin to improve the performance of PVA-based plastic is a feasible method, and it could facilitate the development of high-value utilization of lignin.

Research progress in the preparation of lignin-based carbon nanofibers for supercapacitors using electrospinning technology: A review.

With the development of renewable energy technologies, the demand for efficient energy storage systems is growing. Supercapacitors have attracted considerable attention as efficient electrical energy storage devices because of their excellent power density, fast charging and discharging capabilities, and long cycle life. Carbon nanofibers are widely used as electrode materials in supercapacitors because of their excellent mechanical properties, electrical conductivity, and light weight. Although environmental factors are increasingly driving the application of circular economy concepts in materials science, lignin is an underutilized but promising environmentally benign electrode material for supercapacitors. Lignin-based carbon nanofibers are ideal for preparing high-performance supercapacitor electrode materials owing to their unique chemical stability, abundance, and environmental friendliness. Electrospinning is a well-known technique for producing large quantities of uniform lignin-based nanofibers, and is the simplest method for the large-scale production of lignin-based carbon nanofibers with specific diameters. This paper reviews the latest research progress in the preparation of lignin-based carbon nanofibers using the electrospinning technology, discusses the prospects of their application in supercapacitors, and analyzes the current challenges and future development directions. This is expected to have an enlightening effect on subsequent research.

Baricitinib relieves DSS-induced ulcerative colitis in mice by suppressing the NF-κB and JAK2/STAT3 signalling pathways.

Ulcerative colitis (UC) is a relapsing inflammatory disease with a unique aetiology. The treatment of UC is challenging, and the current clinical therapeutics for colitis have limited efficacy. Thus, finding new and effective treatment options remains urgent. Baricitinib, an inhibitor of Janus kinase (JAK), has been clinically used to treat rheumatoid arthritis (RA). However, its potential effects on UC have not been fully elucidated. In this study, we aimed to explore the effects of baricitinib on UC and its underlying mechanism. Dextran sulphate sodium (DSS)-induced murine model of chronic colitis was used to investigate the intervention efficacy following oral administration of baricitinib. The levels of key cytokines, such as IL-6, IFN-γ and IL-17A, were determined. Moreover, western blotting for IκBα, p-IκBα, JAK2, p-JAK2, STAT3 and p-STAT3 protein expression was performed to investigate the associated signalling pathways. Our findings demonstrated that baricitinib can significantly relieve DSS-induced UC in mice. After baricitinib intervention, IL-6, IFN-γ and IL-17A levels were decreased both in vitro and in vivo. Moreover, the elevated expression levels of p-IκBα, p-JAK2, and p-STAT3 were significantly reduced after treatment. Collectively, these results suggest that baricitinib is a potential therapeutic agent for alleviation of DSS-induced colitis. This study provides a method for subsequent investigations on potential curative drugs development of the for colitis.

LncRNA H19 Participates in Leukemia Inhibitory Factor Mediated Stemness Promotion in Colorectal Cancer Cells.

Colorectal cancer (CRC) is a common human malignancy and the third leading cause of cancer-related death worldwide. Cancer stem cells (CSCs) were considered to play important roles in the genesis and development of many tumors. In recent years, it has been observed that leukemia inhibitory factor (LIF) might be involved in the regulation of stemness in cancer cells. In this study, we observed that LIF could increase the spheroid formation and stemness marker expression (inculding Nanog and SOX2) in CRC cell lines, such as HCT116 and Caco2 cells. Meanwhile, we also observed that LIF could upregulate LncRNA H19 expression via PI3K/AKT pathway. Knockdown of the expression of LncRNA H19 could decrease the spheroid formation and SOX2 expression in LIF-treated HCT116 and Caco2 cells, and thereby LncRNA H19 knockdown could compensate for the stemness enhancement effects induced by LIF. Our results indicated that LncRNA H19 might participate in the stemness promotion of LIF in CRC cells.

Emodin is a Potential Drug Targeting CD44-positive Hepatocellular Cancer.

BACKGROUND: Liver cancer is one of the most prevalent forms of cancer of the digestive system in our country. The most common subtype of this disease is hepatocellular carcinoma (HCC). Currently, treatment options for HCC patients include surgical resection, liver transplantation, radiofrequency ablation, chemoembolization, and biologic-targeted therapy. However, the efficacy of these treatments is suboptimal, as they are prone to drug resistance, metastasis, spread, and recurrence. These attributes are closely related to cancer stem cells (CSCs). Therefore, the utilization of drugs targeting CSCs may effectively inhibit the development and recurrence of HCC. METHODS: HepG2 and Huh7 cells were used to analyze the antitumor activity of emodin by quantifying cell growth and metastasis, as well as to study its effect on stemness. RESULTS: Emodin effectively suppressed the growth and movement of HCC cells. Emodin also significantly inhibited the proliferation of CD44-positive hepatoma cells. CONCLUSION: Emodin shows promise as a potential therapeutic agent for HCC by targeting CD44-- positive hepatoma cells.

The function of MSP-activated γδT cells in hepatocellular carcinoma.

Immunotherapeutic strategies targeting γδT cells are now recognized as a promising treatment method for hepatocellular carcinoma (HCC). To date, no specific antigen or antigenic epitope recognized by γδT cells has been identified, limiting their application in the field of HCC treatment. Previously, we used an established screening strategy to identify a novel HCC protein antigen recognized by γδT cells called MSP. In this study, we explored the function of MSP activated-γδT cells in HCC. Results demonstrated that the proportions of γδT cells in the peripheral blood of HCC patients and the level of IFN-γ in the serum were higher than in healthy controls. We also determined that γδT cells can bind MSP protein. MSP-activated γδT cells were shown to contain a specific CDR3δ2 sequence that supports the recognition of MSP by γδT cells. We determined that MSP is highly expressed in HCC, MSP-activated γδT cells in the peripheral blood of HCC patients express co-stimulatory molecules, and MSP-activated γδT cells directly killed HCC cells. In conclusion, we demonstrated that the novel protein ligand MSP activated γδT cells, leading to the killing of HCC cells through direct and indirect mechanisms. These findings could provide a potential new target for the clinical diagnosis and treatment of HCC and a foundation for clinical treatment strategies in HCC.

Driving factors and decoupling analysis of natural gas consumption in major Organization for Economic Cooperation and Development countries.

Natural gas is regarded as the main transition energy under the carbon-neutral strategy and its main consumers are Organization for Economic Cooperation and Development countries, accounting for 44.5% of world consumption in 2021. In order to investigate the effects of technology, industry, and regions on natural gas consumption, 12 major Organization for Economic Cooperation and Development countries from three different country groups were selected in this paper to explore the consumption change. Firstly, the Logarithmetic Mean Divisia Index model is adopted to find out the driving factors. Then, the Tapio model is used to consider the decoupling state between natural gas consumption and economic growth. The results can be concluded as follows: (a) From 2000 to 2020, the technological progress effect has the biggest values of -148.86, followed by the industrial structure effect and the regional scale effect, with values of - 37.04 and 29.42, respectively. (b) From the perspective of industry view, these three effects have the largest impact on the secondary industry, followed by the tertiary industry and primary industry; (c) the regional scale effect has a positive effect on most countries, and the industrial structure effect and the regional scale effect have a negative effect on most countries; (d) the decoupling state vary differently in countries with different groups. Therefore, we concluded two policy recommendations for nature gas reduction: (a) Technological innovation is the most effective way for reducing natural gas consumption; (b) Industrial structure optimization can help save natural gas consumption.

Preparation and characterization of bifunctional wolfsbane-like magnetic Fe3O4 nanoparticles-decorated lignin-based carbon nanofibers composites for electromagnetic wave absorption and electrochemical energy storage.

Recently, with the pursuit of high-efficiency electromagnetic wave absorption (EMWA) and electrochemical energy storage (EES) materials, multifunctional lignin-based composites have attracted significant interest due to their low cost, vast availability, and sustainability. In this work, lignin-based carbon nanofibers (LCNFs) was first prepared by electrospinning, pre-oxidation and carbonization processes. Then, different content of magnetic Fe3O4 nanoparticles were deposited on the surface of LCNFs via the facile hydrothermal way to produce a series of bifunctional wolfsbane-like LCNFs/Fe3O4 composites. Among them, the synthesized optimal sample (using 12 mmol of FeCl3·6H2O named as LCNFs/Fe3O4-2) displayed excellent EMWA ability. When the minimum reflection loss (RL) value achieved -44.98 dB at 6.01 GHz with an thickness of 1.5 mm, and the effective absorption bandwidth (EAB) was up to 4.19 GHz ranging from 5.10 to 7.21 GHz. For supercapacitor electrode, the highest specific capacitance of LCNFs/Fe3O4-2 reached 538.7 F/g at the current density of 1 A/g, and the capacitance retention remained at 80.3 %. Moreover, an electric double layer capacitor of LCNFs/Fe3O4-2//LCNFs/Fe3O4-2 also showed a remarkable power density of 7755.29 W/kg, outstanding energy density of 36.62 Wh/kg and high cycle stability (96.89 % after 5000 cycles). In short, the construction of this multifunctional lignin-based composites has potential applications in electromagnetic wave (EMW) absorbers and supercapacitor electrodes.

Necroptosis in the pathophysiology of preeclampsia.

Necroptosis is a newly-identified form of gene-regulated cell necrosis that is increasingly considered to be a pathway associated with human pathophysiological conditions. Cells undergoing necroptosis exhibit necrotic phenotypes, including disruption of the plasma membrane integrity, organelle swelling, and cytolysis. Accumulating evidence suggests that trophoblast necroptosis plays a complex role in preeclampsia (PE). However, the exact pathogenesis remains unclear. Its unique mechanisms of action in various diseases are expected to provide prospects for the treatment of PE. Therefore, it is necessary to further explore its molecular mechanism in PE in order to identify potential therapeutic options. This review examines the current knowledge regarding the role and mechanisms of necroptosis in PE and provides a theoretical basis for new therapeutic targets for PE.

N-S co-doping lignin-based carbon magnetic nanoparticles as high performance supercapacitor and electromagnetic wave absorber.

Recently, multifunctional lignin-based materials are gaining more and more attention due to their great potential for low-cost and sustainability. In this work, to obtain both an excellent supercapacitor electrode and an outstanding electromagnetic wave (EMW) absorber, a series of multifunctional nitrogen-sulphur (N-S) co-doped lignin-based carbon magnetic nanoparticles (LCMNPs) had been successfully prepared through Mannich reaction at different carbonization temperature. As compared with the directly carbonized lignin carbon (LC), LCMNPs had more nano-size structure and higher specific surface area. Meanwhile, with the increase of carbonization temperature, the graphitization of the LCMNPs could also be effectively improved. Therefore, LCMNPs-800 displayed the best performance advantages. For the electric double layer capacitor (EDLC), the optimal specific capacitance of LCMNPs-800 reached 154.2 F/g, and the capacitance retention after 5000 cycles was as high as 98.14 %. When the power density was 2204.76 W/kg, the energy density achieved 33.81 Wh/kg. In addition, N-S co-doped LCMNPs also exhibited strong electromagnetic wave absorption (EMWA) ability, whose the minimum reflection loss (RL) value of LCMNPs-800 was realized -46.61 dB at 6.01 GHz with an thickness of 4.0 mm, and the effective absorption bandwidth (EAB) was up to 2.11 GHz ranging from 5.10 to 7.21 GHz, which could cover the C-band. Overall, this green and sustainable approach is a promising strategy for the preparation of high-performance multifunctional lignin-based materials.

A functional lignin for heavy metal ions adsorption and wound care dressing.

Recently, the application of lignin activation by demethylation to improve reactivity and enrich multiple functions has intensively attracted attention. However, it is still challenge up to now due to the low reactivity and complexity of lignin structure. Here, an effective demethylation way was explored by microwave-assisted method for substantially enhancing the hydroxyl (-OH) content and retaining the structure of lignin. Then, the optimum demethylated lignin was used to removal heavy metal ions and promote wound healing, respectively. In detail, for microwave-assisted demethylated poplar lignin (M-DPOL), the contents of phenolic (Ar-OH) and total hydroxyl (Tot-OH) groups reached the maximum for 60 min at 90 °C in DMF with 7.38 and 9.13 mmol/g, respectively. After demethylation, with this M-DPOL as lignin-based adsorbent, the maximum adsorption capacity (Qmax) for Pb2+ ions reached 104.16 mg/g. Based on the isotherm, kinetic and thermodynamic models analyses, the chemisorption occurred in monolayer on the surface of M-DPOL, and all adsorption processes were endothermic and spontaneous. Meanwhile, M-DPOL as a wound dressing had excellent antioxidant property, outstanding bactericidal activity and remarkable biocompatibility, suggesting that it did not interfere with cell proliferation. Besides, the wounded rats treated with M-DPOL significantly promoted its formation of re-epithelialization and wound healing of full-thickness skin defects. Overall, microwave-assisted method of demethylated lignin can offer great advantages for heavy metal ions removal and wound care dressing, which facilitates high value application of lignin.

A pH-Sensitive Lignin-Based Material for Sustained Release of 8-Hydroxyquinoline.

The fabrication of pH-sensitive lignin-based materials has received considerable attention in various fields, such as biomass refining, pharmaceuticals, and detecting techniques. However, the pH-sensitive mechanism of these materials is usually depending on the hydroxyl or carboxyl content in the lignin structure, which hinders the further development of these smart materials. Here, a pH-sensitive lignin-based polymer with a novel pH-sensitive mechanism was constructed by establishing ester bonds between lignin and the active molecular 8-hydroxyquinoline (8HQ). The structure of the produced pH-sensitive lignin-based polymer was comprehensively characterized. The substituted degree of 8HQ was tested up to 46.6% sensitivity, and the sustained release performance of 8HQ was confirmed by the dialysis method, the sensitivity of which was found to be 60 times slower compared with the physical mixed sample. Moreover, the obtained pH-sensitive lignin-based polymer showed an excellent pH sensitivity, and the released amount of 8HQ under an alkaline condition (pH = 8) was obviously higher than that under an acidic condition (pH = 3 and 5). This work provides a new paradigm for the high-value utilization of lignin and a theory guidance for the fabrication of novel pH-sensitive lignin-based polymers.

Designed synthesis of multifunctional lignin-based adsorbent for efficient heavy metal ions removal and electromagnetic wave absorption.

Multifunctional lignin-based adsorbents, which have shown great application prospect, have attracted widespread attention. Herein, a series of multifunctional lignin-based magnetic recyclable adsorbents were prepared from carboxymethylated lignin (CL), which was rich in carboxyl group (-COOH). After optimizing the mass ratio of CL to Fe3O4, the prepared CL/Fe3O4 (3:1) adsorbent showed efficient adsorption capacities for heavy metal ions. The kinetic and isotherm nonlinear fitting studies revealed that the adsorption process followed the second-order kinetic and Langmuir models, and the maximum adsorption capacities (Qmax) of CL/Fe3O4 (3:1) magnetic recyclable adsorbent for Pb2+, Cu2+ and Ni2+ ions reached 189.85, 124.43 and 106.97 mg/g, respectively. Meanwhile, after 6 cycles, the adsorption capacities of CL/Fe3O4 (3:1) for Pb2+, Cu2+ and Ni2+ ions could keep at 87.4 %, 83.4 % and 82.3 %, respectively. In addition, CL/Fe3O4 (3:1) also exhibited excellent electromagnetic wave absorption (EMWA) performance with a reflection loss (RL) of -28.65 dB at 6.96 GHz under the thickness of 4.5 mm, and its effective absorption bandwidth (EAB) achieved 2.24 GHz (6.08-8.32 GHz). In short, the prepared multifunctional CL/Fe3O4 (3:1) magnetic recyclable adsorbent with outstanding adsorption capacity for heavy metal ions and superior EMWA capability opens a new avenue for the diversified utilization of lignin and lignin-based adsorbent.

Leukemia Inhibitory Factor Impairs the Function of Peripheral γδT Cells in Patients with Colorectal Cancer.

Immunotherapeutic strategies are recognized as promising treatment methods for colorectal cancer (CRC). αßT cell-mediated cytotoxicity is tolerated by cancer cells with low MHC class I expression; therefore, γδT cell-based cancer immunotherapy has generated increasing interest as a potential treatment option. To enhance the potency of γδT cell-based immunotherapy, the key factors involved in the regulation of γδT cells in CRC need to be identified along with devising ways to overcome potential hurdles. In this study, we observed that leukemia inhibitory factor (LIF), the serum level of which was highly increased in those with solid tumors, could specifically attenuate the cytotoxic function of peripheral γδT cells in patients with CRC. We observed that in patients with CRC, the expression levels of perforin and granzyme were significantly decreased in the recombinant human LIF (rhLIF)-treated peripheral γδT cells, whereas that of the LIF receptor (LIFR) was higher. The regulation of the cytotoxic function of the γδT cells by rhLIF was effected mainly through the STAT3 signaling pathway, which caused an increase in the expression levels of interleukin (IL)-17, COX-2, and prostaglandin E2 (PGE2). Our results revealed that rhLIF could impair the function of γδT cells in CRC patients by reducing the cytotoxic function and increasing the expression of tumor-promoting molecules, such as IL-17, COX-2, and PGE2.

MCP-1/CCR2 axis is involved in the regulation of γδT cells in lupus nephritis.

γδT cells are important innate immune cells that are involved in the occurrence and development of autoimmune diseases such as systemic lupus erythematosus (SLE). Lupus nephritis (LN) is a serious complication of SLE, characterized by the accumulation of immune cells (including γδT cells) in the target organs to participate in the disease process. Therefore, clarifying how γδT cells chemotactically migrate to target organs may be a key to developing therapeutic methods against LN. Enzyme-linked immunosorbent assay (ELISA) was used to detect serum levels of chemokines in LN patients and healthy controls. Real-time polymerase chain reaction (RT-PCR) and flow cytometry were used to measure the expression of chemokine receptors on the surface of γδT cells. The chemotactic migration ability of γδT cells was detected by Transwell assay. Signalling pathway activation of γδT cells was detected by Automated Capillary Electrophoresis Immunoassay and flow cytometry. The serum levels of chemokines, including monocyte chemoattractant protein-1 (MCP-1) in LN patients, were significantly increased. CCR2, the receptor of MCP-1, was also highly expressed on the surface of peripheral γδT cells in LN patients. In addition, the exogenous addition of MCP-1 can enhance chemotactic migration of γδT cells in LN patients. MCP-1 could activate STAT3 signalling in LN patients' peripheral γδT cells. γδT cells might participate in the pathogenesis of LN through MCP-1/CCR2 axis. This finding provides new opportunities for developing treatment methods against LN by targeting MCP-1/CCR2 axis.

Preparation of activated lignin with high hydroxyl content using lewis acid as demethylation reagent.

Activation of lignin by demethylation for improving the reactivity has attracted extensive attentions. However, it still faces many challenges, such as the unsatisfied increase of hydroxyl content and the undesired cracking of linear linkages. Here, the efficient demethylations for significantly increasing the hydroxyl content and protecting the structure of industrial lignin were explored using lewis acid as modification reagent. As BBr3 was used, the phenolic hydroxyl content (Ar-OH) was increased by 80.65 %, but the lignin structure might be destroyed. About 75 % of the ß-O-4 linkages could be fortunately retained by using AlCl3. This method could also be used for the demethylation of alkaline poplar lignin with up to 171.67 % increase of Ar-OH (from 1.80 to 4.89 mmol/g). After activation, the antioxidant properties were improved 4.64-fold and 2.58-fold for 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals, respectively. This work would provide a theory guidance for activation of lignin and facilitate its high-value application.

Grave-to-cradle upcycling of Ni from electroplating wastewater to photothermal CO2 catalysis.

Treating hazardous waste Ni from the electroplating industry is mandated world-wide, is exceptionally expensive, and carries a very high CO2 footprint. Rather than regarding Ni as a disposable waste, the chemicals and petrochemicals industries could instead consider it a huge resource. In the work described herein, we present a strategy for upcycling waste Ni from electroplating wastewater into a photothermal catalyst for converting CO2 to CO. Specifically, magnetic nanoparticles encapsulated in amine functionalized porous SiO2, is demonstrated to efficiently scavenge Ni from electroplating wastewater for utilization in photothermal CO2 catalysis. The core-shell catalyst architecture produces CO at a rate of 1.9 mol·gNi-1·h-1 (44.1 mmol·gcat-1·h-1), a selectivity close to 100%, and notable long-term stability. This strategy of upcycling metal waste into functional, catalytic materials offers a multi-pronged approach for clean and renewable energy technologies.

Non-structure protein ORF1ab (NSP8) in SARS-CoV-2 contains potential γδT cell epitopes.

Upon activation by the pathogen through T-cell receptors (TCRs), γδT cells suppress the pathogenic replication and thus play important roles against viral infections. Targeting SARS-CoV-2 via γδT cells provides alternative therapeutic strategies. However, little is known about the recognition of SARS-CoV-2 antigens by γδT cells. We discovered a specific Vγ9/δ2 CDR3 by analyzing γδT cells derived from the patients infected by SARS-CoV-2. Using a cell model exogenously expressing γδ-TCR established, we further screened the structural motifs within the CDR3 responsible for binding to γδ-TCR. Importantly, these sequences were mapped to NSP8, a non-structural protein in SARS-CoV-2. Our results suggest that NSP8 mediates the recognition by γδT cells and thus could serve as a potential target for vaccines.

Ultrasonic assisted enhanced catalytic effect of perovskite to promote depolymerization of lignin.

The search for renewable energy sources to replace fossil fuel has made lignin a promising carbon-containing resource. In this paper, LaNiO3 perovskite catalyst supported by mesoporous carrier with specific pore structure was prepared by the pore filling of MCM-41 with citrate complex precursors of nickel and lanthanum. Then the catalysts applied to maize straw lignin depolymerization. The results of low-angle XRD, N2 adsorption-desorption, IR spectroscopy and SEM confirmed that the catalyst has been successfully manufactured. Based on the yield of phenolic monomer, low molecular weight lignin derived bio-oil and high molecular weight lignin derived bio-oil as standard, the catalyst showed best catalytic effect when the reaction temperature was 250 °C, the reaction time was 6 h, the ratio of lignin to catalyst mass was 5: 1 and with ultrasonic assist. The yield of phenolic monomer was 11.46 wt% and that of bio-oil was 68.0 wt%. In general, this method is an excellent embodiment of the principle of Lignin-first as well as an excellent strategy for the production of value-added phenolics and high-quality bio-oils from lignin. It plays an important role in promoting the high value utilization of lignin in the future.