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1.
Front Public Health ; 12: 1373044, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38601492

RESUMEN

Objectives: To investigate the causal relationships between pneumoconiosis and rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), and gout. Methods: The random-effects inverse variance weighted (IVW) approach was utilized to explore the causal effects of the instrumental variables (IVs). Sensitivity analyses using the MR-Egger and weighted median (WM) methods were did to investigate horizontal pleiotropy. A leave-one-out analysis was used to avoid the bias resulting from single-nucleotide polymorphisms (SNPs). Results: There was no causal association between pneumoconiosis and SLE, RA or gout in the European population [OR = 1.01, 95% CI: 0.94-1.10, p = 0.74; OR = 1.00, 95% CI: 0.999-1.000, p = 0.50; OR = 1.00, 95% CI: 1.000-1.001, p = 0.55]. Causal relationships were also not found in pneumoconiosis due to asbestos and other mineral fibers and SLE, RA and gout [OR = 1.01, 95% CI: 0.96-1.07, p = 0.66; OR = 1.00, 95% CI: 1.00-1.00, p = 0.68; OR = 1.00, 95% CI: 1.00-1.00, p = 0.20]. Conclusion: Our study suggests that pneumoconiosis may have no causal relationship with the three inflammatory immune diseases.


Asunto(s)
Gota , Enfermedades del Sistema Inmune , Lupus Eritematoso Sistémico , Neumoconiosis , Humanos , Análisis de la Aleatorización Mendeliana , Neumoconiosis/epidemiología
2.
Environ Res ; 231(Pt 2): 116222, 2023 08 15.
Artículo en Inglés | MEDLINE | ID: mdl-37224951

RESUMEN

Endocrine-disrupting chemicals (EDCs) widely exist in people's production and life which have great potential to damage human and animal health. Over the past few decades, growing attention has been paid to the impact of EDCs on human health, as well as immune system. So far, researchers have proved that EDCs (such as bisphenol A (BPA), phthalate, tetrachlorodibenzodioxin (TCDD), etc.) affect human immune function and promotes the occurrence and development of autoimmune diseases (ADs). Therefore, in order to better understand how EDCs affect ADs, we summarized the current knowledge about the impact of EDCs on ADs, and elaborated the potential mechanism of the impact of EDCs on ADs in this review.


Asunto(s)
Enfermedades Autoinmunes , Disruptores Endocrinos , Dibenzodioxinas Policloradas , Animales , Humanos , Disruptores Endocrinos/toxicidad , Enfermedades Autoinmunes/inducido químicamente , Enfermedades Autoinmunes/epidemiología , Sistema Inmunológico
3.
Autoimmun Rev ; 22(2): 103234, 2023 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-36423833

RESUMEN

Nearly 20 years of studies have shown that fungi and the human immune system (non-specific immunity and specific immunity) and bacterial--fungal interactions maintain a balance that can't lead to diseases. Fungi--microorganism that lives in human intestine--may play an important role in human health and disease. Population studies and animal models in some diseases have found the changes in the diversity and composition of fungi. The dysregulation of the fungi can disrupt the normal "running" of the immune system and bacteria, which triggers the development of inflammatory diseases. The latest studies of fungi in inflammatory bowel disease, systemic lupus erythematosus, ankylosing spondylitis and type 1 diabetes mellitus were summarized. This review considers how the healthy host protect against the potential harm of intestinal fungi through the immune system and how fungal dysregulation alters host immunity.


Asunto(s)
Enfermedades Autoinmunes , Lupus Eritematoso Sistémico , Espondilitis Anquilosante , Animales , Humanos , Intestinos , Inmunidad Innata , Bacterias , Hongos , Enfermedades Autoinmunes/etiología
4.
Sci Total Environ ; 859(Pt 1): 160244, 2023 Feb 10.
Artículo en Inglés | MEDLINE | ID: mdl-36402344

RESUMEN

BACKGROUND: The relationship between greenness and health emerges as new public health concern. More published studies from multiple areas have explored the relationship between greenness and allergic rhinitis (AR) in children and adolescents. This study aims to determine the association between greenness and allergic rhinitis by systematic review and meta-analysis, in order to provide a more comprehensive assessment of the impact of greenness on AR in children and adolescents. METHODS: The relative literature was systematically searched in PubMed, Embase, and Web of science lastly on September 25, 2022. Terms related to greenness and allergic rhinitis were used for searching. Summary effect estimates of greenness on AR in children and adolescents were calculated for per 10 % increase of greenness exposure with different buffer sizes by random-effects model. RESULTS: A total of 579 studies were screened, and fourteen studies from Europe, Asia and North America were finally included. Most greenness exposure were measured by normalized difference vegetation index (NDVI). Enhanced vegetation index, outdoor-green environmental score and existed to measuring different greenness types. Greenness surrounding residences and schools were assessed. The overall effect of greenness on primary outcome was 1.00 (95%CI = 0.99-1.00). Most effect estimates of greenness were included in the NDVI-500 m group, and the pooled OR was 0.99 (95%CI = 0.97-1.01). No significant pooled estimates were found in analyses with study locations. CONCLUSION: This study indicates no significant association between greenness exposure and AR in children and adolescents. Various exposure measures and conversion of data may affect the results of this meta-analysis. More precise assessment of personal greenness exposure in well-designed prospective studies are vital for drawing a definite association in future. Furthermore, greenness exposure surrounding schools should be paid considerable attention for its effect on AR in school-aged children and adolescents.


Asunto(s)
Rinitis Alérgica , Niño , Adolescente , Humanos , Estudios Prospectivos , Rinitis Alérgica/epidemiología , Vivienda , Instituciones Académicas , Asia
6.
J Neurol ; 266(12): 3119-3125, 2019 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-31535268

RESUMEN

OBJECTIVE: Improvement of quality-of-life (QoL) has been termed as a primary objective in initiating therapy in both Parkinson's disease (PD) and multiple system atrophy Parkinsonian subtype (MSA-P). We aimed to compare the determinants of life quality in drug naïve PD and MSA-P patients. METHODS: Eighty-six drug-naïve PD patients and thirty-five drug-naïve MSA-P patients were included to explore the determinants of QoL. Demographic information, motor deficits, and non-motor symptoms were included in the clinical assessment. RESULTS: Both motor and non-motor functions were more severely impaired in the drug-naïve MSA-P patients, with higher PDQ-39 scores indicating poorer QoL. Physical discomfort and stigma were the main affected sub-domains in PD, while mobility and activity of daily life were the main affected ones in MSA-P. BECK depressive scores and UPDRS-III scores were independent variables of PDQ-39 in MSA-P patients. Age, depression, disease stages and non-motor scores were independent variables of PDQ-39 in PD patients. INTERPRETATION: Drug-naïve MSA-P patients suffered from more severe motor and non-motor disability, as well as poorer QoL. Depression and non-motor symptoms were proved to be the most critical determinants for QoL in PD, while motor function was supposed to be the major determinant for MSA-P. When initiating therapy, physicians need to focus more on motor functions in drug-naïve MSA-P patients, but on depression in PD patients.


Asunto(s)
Trastorno Depresivo/fisiopatología , Discinesias/fisiopatología , Atrofia de Múltiples Sistemas/fisiopatología , Trastornos Parkinsonianos/fisiopatología , Calidad de Vida , Anciano , Trastorno Depresivo/etiología , Quimioterapia/normas , Discinesias/etiología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Atrofia de Múltiples Sistemas/complicaciones , Enfermedad de Parkinson/complicaciones , Enfermedad de Parkinson/fisiopatología , Trastornos Parkinsonianos/complicaciones
7.
Cell Res ; 24(9): 1067-90, 2014 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-25081058

RESUMEN

The tyrosine phosphorylation barcode encoded in C-terminus of HER2 and its ubiquitination regulate diverse HER2 functions. PTPN18 was reported as a HER2 phosphatase; however, the exact mechanism by which it defines HER2 signaling is not fully understood. Here, we demonstrate that PTPN18 regulates HER2-mediated cellular functions through defining both its phosphorylation and ubiquitination barcodes. Enzymologic characterization and three crystal structures of PTPN18 in complex with HER2 phospho-peptides revealed the molecular basis for the recognition between PTPN18 and specific HER2 phosphorylation sites, which assumes two distinct conformations. Unique structural properties of PTPN18 contribute to the regulation of sub-cellular phosphorylation networks downstream of HER2, which are required for inhibition of HER2-mediated cell growth and migration. Whereas the catalytic domain of PTPN18 blocks lysosomal routing and delays the degradation of HER2 by dephosphorylation of HER2 on pY(1112), the PEST domain of PTPN18 promotes K48-linked HER2 ubiquitination and its rapid destruction via the proteasome pathway and an HER2 negative feedback loop. In agreement with the negative regulatory role of PTPN18 in HER2 signaling, the HER2/PTPN18 ratio was correlated with breast cancer stage. Taken together, our study presents a structural basis for selective HER2 dephosphorylation, a previously uncharacterized mechanism for HER2 degradation and a novel function for the PTPN18 PEST domain. The new regulatory role of the PEST domain in the ubiquitination pathway will broaden our understanding of the functions of other important PEST domain-containing phosphatases, such as LYP and PTPN12.


Asunto(s)
Dominio Catalítico , Proteínas Tirosina Fosfatasas no Receptoras/química , Proteínas Tirosina Fosfatasas no Receptoras/metabolismo , Receptor ErbB-2/metabolismo , Ubiquitinación , Secuencias de Aminoácidos , Secuencia de Aminoácidos , Neoplasias de la Mama/metabolismo , Neoplasias de la Mama/patología , Línea Celular Tumoral , Cristalografía por Rayos X , Femenino , Humanos , Interacciones Hidrofóbicas e Hidrofílicas , Cinética , Lisina/metabolismo , Lisosomas/metabolismo , Modelos Biológicos , Datos de Secuencia Molecular , Estadificación de Neoplasias , Péptidos/metabolismo , Fosforilación , Fosfotirosina/metabolismo , Complejo de la Endopetidasa Proteasomal/metabolismo , Proteolisis , Transducción de Señal , Relación Estructura-Actividad , Especificidad por Sustrato , Proteínas con Repetición de beta-Transducina/metabolismo
8.
Artículo en Inglés | MEDLINE | ID: mdl-24588753

RESUMEN

The cDNA fragments of hnRNPA2/B1 were cloned from the giant panda and black bear using RT-PCR method, which were, respectively, 1029bp and 1026bp in length encoding 343 and 341 amino acids. Analysis indicated the cDNA cloned from the giant panda encoded variant B1 while the cDNA cloned from black bear encoded variant A2. Analyzing the hnRNPA2B1 peptide of the giant panda and black bear, 76 glycine residues and 86 glycine residues were, respectively, found, and moreover, most glycine are concentrated in the latter halves of the hnRNPA2B1 peptides. Functional sites prediction also showed many N-myristoylation sites existed in the glycine-rich domain, which is probably related to the role of telomere maintenance. From base bias and substitution analysis, we can conclude that the ORF of hnRNPA2/B1 biased G while hated C, and transition of the third site did not achieve the level of saturation. Orthology analysis indicated that both the nucleotide sequence and the deduced amino acid sequence showed high identity to other 26 hnRNPA2/B1 sequences from mammals and nonmammals reported. These sequences were used to construct phylogenetic trees employing the NJ method with 1000 bootstrap, and the obtained tree demonstrated similar topology with the classical systematics, which suggested the potential value of hnRNPA2/B1 in phylogenetic analysis. This report will be the first step to the study function of hnRNPA2/B1 in the giant panda and black bear, and will provide a scientific basis to disease surveillance, captive breeding, and conservation of the endangered species.


Asunto(s)
Ribonucleoproteína Heterogénea-Nuclear Grupo A-B/genética , Filogenia , Animales , Secuencia de Bases , Ursidae/genética
9.
Ying Yong Sheng Tai Xue Bao ; 24(2): 319-25, 2013 Feb.
Artículo en Chino | MEDLINE | ID: mdl-23705373

RESUMEN

According to the 6th Second Class Forest Resource Inventory data in Linzhi area of Tibet, and by using the volume source biomass method and average-biomass computing method, in combining with the molecular formula carbon rate of different tree species, this paper estimated the carbon storage and carbon density of different standing forests and their components in the area, and analyzed the spatial distribution patterns of the carbon storage and carbon density. In 2004, the forest carbon storage in the area was 2. 43x10(8) t, and the average forest carbon density was 76.01 t.hm-2. The carbon storage followed the sequence of standing forest > shrub forest > open forest > scattered trees > bamboo forest > four-side trees. Standing forests had a carbon storage 2.51x10(5)-1.27x10(8) t, accounting for 92.0% of the total forest carbon storage in the area. The average carbon density of various standing forests was 103.16 t.hm-2, and fir forest had the highest carbon storage and carbon density. From the view of regional distribution, the forest carbon storage presented a trend of increased from northwest to southeast, whereas the forest carbon density tended to be increased from southwest to northeast. The carbon storage of the standing forests was mainly consisted by mature and over-matured forests, and the carbon density of over-matured forests was the highest among all the age classes' forests. The forest carbon storage in Linzhi area would be increased with the increase of over-matured forests, but tended to be decreased with the death and decomposition of over-matured forests.


Asunto(s)
Carbono/metabolismo , Ecosistema , Suelo/química , Árboles/crecimiento & desarrollo , Árboles/metabolismo , Abies/crecimiento & desarrollo , Abies/metabolismo , Carbono/análisis , Tibet , Árboles/clasificación
10.
Mol Biol Rep ; 40(2): 1495-8, 2013 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-23070920

RESUMEN

The Giant Panda is an endangered and valuable gene pool in genetic, its important functional gene POLR2H encodes an essential shared peptide H of RNA polymerases. The genomic DNA and cDNA sequences were cloned successfully for the first time from the Giant Panda (Ailuropoda melanoleuca) adopting touchdown-PCR and reverse transcription polymerase chain reaction (RT-PCR), respectively. The length of the genomic sequence of the Giant Panda is 3,285 bp, including five exons and four introns. The cDNA fragment cloned is 509 bp in length, containing an open reading frame of 453 bp encoding 150 amino acids. Alignment analysis indicated that both the cDNA and its deduced amino acid sequence were highly conserved. Protein structure prediction showed that there was one protein kinase C phosphorylation site, four casein kinase II phosphorylation sites and one amidation site in the POLR2H protein, further shaping advanced protein structure. The cDNA cloned was expressed in Escherichia coli, which indicated that POLR2H fusion with the N-terminally His-tagged form brought about the accumulation of an expected 20.5 kDa polypeptide in line with the predicted protein. On the basis of what has already been achieved in this study, further deep-in research will be conducted, which has great value in theory and practical significance.


Asunto(s)
Subunidades de Proteína/genética , ARN Polimerasa II/genética , Ursidae/genética , Animales , Clonación Molecular , ADN Complementario/genética , Escherichia coli , Genoma , Modelos Moleculares , Estructura Terciaria de Proteína , Subunidades de Proteína/biosíntesis , ARN Polimerasa II/biosíntesis , Análisis de Secuencia de ADN
11.
Mol Biol Rep ; 39(5): 5761-6, 2012 May.
Artículo en Inglés | MEDLINE | ID: mdl-22212708

RESUMEN

ATP6V1F encodes a component of vacuolar ATPase mediating acidification. The cDNA and the genomic sequences of ATP6V1F were cloned successfully for the first time from the Giant Panda (Ailuropoda melanoleuca) using reverse transcription polymerase chain reaction and touchdown-polymerase chain reaction, respectively. The cDNA fragment cloned is 364 bp in size, containing an open reading frame of 360 bp encoding 119 amino acids. Alignment analysis indicated that both ORF and the deduced amino acid sequence are highly conserved. The length of the genomic sequence of the Giant Panda is 2225 bp, including two exons and one intron. Topology prediction showed that there is one protein kinase C phosphorylation site, two Casein kinase II phosphorylation sites, and one N-myristoylation site in the ATP6V1F protein. The ATP6V1F gene was overexpressed in Escherichia coli indicating that ATP6V1F fusion with the N-terminally His-tagged form gave rise to the accumulation of an expected 17 kDa polypeptide, which was according with the predicted protein and also could be used to purify the protein and study its function.


Asunto(s)
Regulación Enzimológica de la Expresión Génica , Ursidae/genética , ATPasas de Translocación de Protón Vacuolares/genética , ATPasas de Translocación de Protón Vacuolares/metabolismo , Secuencia de Aminoácidos , Aminoácidos/metabolismo , Animales , Secuencia de Bases , Clonación Molecular , ADN Complementario/genética , Escherichia coli/metabolismo , Genoma/genética , Datos de Secuencia Molecular , Unión Proteica , Estructura Secundaria de Proteína , Análisis de Secuencia de ADN , ATPasas de Translocación de Protón Vacuolares/química
12.
Guang Pu Xue Yu Guang Pu Fen Xi ; 31(8): 2036-9, 2011 Aug.
Artículo en Chino | MEDLINE | ID: mdl-22007379

RESUMEN

High temperature annealing and Cs/O activation are external incentives, while the property of GaN material is internal factor in the preparation of negative electron affinity GaN photocathode. The similarities and differences of the performance of the two structure photocathodes are analysed based on the difference of the structure between uniform-doping and gradient-doping negative electron affinity GaN photocathodes and the changes in photocurrents in activation and the quantum yield after successfully activated of GaN photocathodes. Experiments show that: the photocurrent growth rate is slower in activation, activation time is longer and quantum efficiency is higher after successfully activated of gradient-doping GaN photocathode than those of uniform-doping photocathode respectively. The field-assisted photocathode emission model can explain the differences between the two, built-in electric field of gradient-doping structure creates additional electronic drift to the photocathode surface, and the probability of electrons to reach the photocathode surface is improved correspondingly.

13.
Mol Biol (Mosk) ; 44(2): 235-42, 2010.
Artículo en Inglés | MEDLINE | ID: mdl-20586183

RESUMEN

GAPDH (glyceraldehyde-3-phosphate dehydrogenase) is a key enzyme of the glycolytic pathway and it is related to the occurrence of some diseases. The cDNA and the genomic sequence of GAPDH were cloned successfully from the Giant Panda (Ailuropoda melanoleuca) using the RT-PCR technology and Touchdown-PCR, respectively. Both sequences were analyzed preliminarily. The cDNA of GAPDH cloned from the Giant Panda is 1191 bp in size, contains an open reading frame of 1002 bp encoding 333 amino acids. The genomic sequence is 3941 bp in length and was found to possess 10 exons and 9 introns. Alignment analysis indicates that the nucleotide sequence and the deduced amino acid sequence are highly conserved in some mammalian species, including Homo sapiens, Mu musculus, Rattus norvegicus, Canis lupus familiaris and Bos taurus. The homologies for the nucleotide sequences of the Giant Panda GAPDH to that of these species are 90.67, 90.92, 90.62, 95.01 and 92.32% respectively, while the homologies for the amino acid sequences are 94.93, 95.5, 95.8, 98.8 and 97.0%. Primary structure analysis revealed that the molecular weight of the putative GAPDH protein is 35.7899 kDa with a theoretical pI of 8.21. Topology prediction showed that there is one Glyceraldehyde 3-phosphate dehydrogenase active site, two N-glycosylation sites, four Casein kinase II phosphorylation sites, seven Protein kinase C phosphorylation sites and eight N-myristoylation sites in the GAPDH protein of the Giant Panda. The GAPDH gene was overexpressed in E. coli BL21. The results indicated that the fusion of GAPDH with the N-terminally His-tagged form gave rise to the accumulation of an expected 43 kDa polypeptide. The SDS-PAGE analysis also showed that the recombinant GAPDH was soluble and thus could be used for further functional studies.


Asunto(s)
ADN Complementario/genética , Gliceraldehído-3-Fosfato Deshidrogenasas/genética , Ursidae/genética , Secuencia de Aminoácidos , Animales , Clonación Molecular , Gliceraldehído-3-Fosfato Deshidrogenasas/metabolismo , Humanos , Procesamiento Proteico-Postraduccional/fisiología , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Ursidae/metabolismo
14.
Mol Biol Rep ; 36(8): 2139-45, 2009 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-19101821

RESUMEN

RPS25 is a component of the 40S small ribosomal subunit encoded by RPS25 gene, which is specific to eukaryotes. Studies in reference to RPS25 gene from animals were handful. The Giant Panda (Ailuropoda melanoleuca), known as a "living fossil", are increasingly concerned by the world community. Studies on RPS25 of the Giant Panda could provide scientific data for inquiring into the hereditary traits of the gene and formulating the protective strategy for the Giant Panda. The cDNA of the RPS25 cloned from Giant Panda is 436 bp in size, containing an open reading frame of 378 bp encoding 125 amino acids. The length of the genomic sequence is 1,992 bp, which was found to possess four exons and three introns. Alignment analysis indicated that the nucleotide sequence of the coding sequence shows a high homology to those of Homo sapiens, Bos taurus, Mus musculus and Rattus norvegicus as determined by Blast analysis, 92.6, 94.4, 89.2 and 91.5%, respectively. Primary structure analysis revealed that the molecular weight of the putative RPS25 protein is 13.7421 kDa with a theoretical pI 10.12. Topology prediction showed there is one N-glycosylation site, one cAMP and cGMP-dependent protein kinase phosphorylation site, two Protein kinase C phosphorylation sites and one Tyrosine kinase phosphorylation site in the RPS25 protein of the Giant Panda. The RPS25 gene was overexpressed in E. coli BL21 and Western Blotting of the RPS25 protein was also done. The results indicated that the RPS25 gene can be really expressed in E. coli and the RPS25 protein fusioned with the N-terminally his-tagged form gave rise to the accumulation of an expected 17.4 kDa polypeptide. The cDNA and the genomic sequence of RPS25 were cloned successfully for the first time from the Giant Panda using RT-PCR technology and Touchdown-PCR, respectively, which were both sequenced and analyzed preliminarily; then the cDNA of the RPS25 gene was overexpressed in E. coli BL21 and immunoblotted, which is the first report on the RPS25 gene from the Giant Panda. The data will enrich and supplement the information about RPS25, which will contribute to the protection for gene resources and the discussion of the genetic polymorphism.


Asunto(s)
Proteínas Ribosómicas/genética , Ursidae/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Clonación Molecular , ADN Complementario/genética , Escherichia coli/genética , Genoma , Datos de Secuencia Molecular , Músculo Esquelético/química , Reacción en Cadena de la Polimerasa , Proteínas Recombinantes de Fusión/biosíntesis , Proteínas Recombinantes de Fusión/genética , Proteínas Ribosómicas/biosíntesis , Alineación de Secuencia
15.
Recent Pat DNA Gene Seq ; 2(1): 16-9, 2008.
Artículo en Inglés | MEDLINE | ID: mdl-19075940

RESUMEN

The cDNA of RPS15 was cloned successfully for the first time from the Giant Panda using RT-PCR technology, which was also sequenced and analyzed preliminarily. The cDNA fragment is 442bp in size, containing an open reading frame (ORF) of 438bp encoding 145 amino acids. Alignment analysis indicates that the nucleotide sequence and the deduced amino acid sequence show a high homology to those of human and other mammalian species reported. Topology prediction shows there are two cAMP and cGMP-dependent kinase phosphorylation sites, one Ribosomal Protein S19 signature site, four N- myristoylation sites and five Casein kinase C phosphorylation sites in the RPS15 protein. Further analysis indicates that the expression sequence of RPS15 and the protein encoded are highly homologous to those of human and other mammals reported. The study is of significance to provide truthful and scientific data for studying the Giant Panda (Ailuropoda melanoleuca) at molecular level, especially for cloning and further researching the complete sequence of RPS15.


Asunto(s)
Proteínas Ribosómicas/química , Proteínas Ribosómicas/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , ADN Complementario , Sistemas de Lectura Abierta , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Alineación de Secuencia , Análisis de Secuencia de ADN , Ursidae
16.
Int J Biol Sci ; 3(7): 428-33, 2007 Oct 26.
Artículo en Inglés | MEDLINE | ID: mdl-18071584

RESUMEN

RPLP1 is one of acidic ribosomal phosphoproteins encoded by RPLP1 gene, which plays an important role in the elongation step of protein synthesis. The cDNA of RPLP1 was cloned successfully for the first time from the Giant Panda (Ailuropoda melanoleuca) using RT-PCR technology, which was also sequenced, analyzed preliminarily and expressed in E.coli. The cDNA fragment cloned is 449bp in size, containing an open reading frame of 344bp encoding 114 amino acids. Alignment analysis indicated that the nucleotide sequence and the deduced amino acid sequence are highly conserved to other five species studied, including Homo sapiens, Mus musculus, Rattus norvegicus, Bos Taurus and Sus scrofa. The homologies for nucleotide sequences of Giant Panda PPLP1 to that of these species are 92.4%, 89.8%, 89.0%, 91.3% and 87.5%, while the homologies for amino acid sequences are 96.5%, 94.7%, 95.6%, 96.5% and 88.6%. Topology prediction showed there are three Casein kinase II phosphorylation sites and two N-myristoylation sites in the RPLP1 protein of the Giant Panda (Ailuropoda melanoleuca). The RPLP1 gene was overexpressed in E. coli and the result indicated that RPLP1 fusion with the N-terminally His-tagged form gave rise to the accumulation of an expected 18kDa polypeptide, which was in accordance with the predicted protein and could also be used to purify the protein and study its function.


Asunto(s)
Fosfoproteínas/genética , Proteínas Ribosómicas/genética , Ursidae/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Conservación de los Recursos Naturales , ADN Complementario/química , Escherichia coli/genética , Expresión Génica , Humanos , Mamíferos/genética , Datos de Secuencia Molecular , Peso Molecular , Músculo Esquelético/metabolismo , Fosfoproteínas/química , Fosfoproteínas/metabolismo , Plásmidos/genética , Plásmidos/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Proteínas Ribosómicas/química , Proteínas Ribosómicas/metabolismo , Análisis de Secuencia de ADN , Homología de Secuencia de Aminoácido , Homología de Secuencia de Ácido Nucleico , Relación Estructura-Actividad , Transformación Bacteriana , Ursidae/metabolismo
17.
DNA Cell Biol ; 26(11): 799-802, 2007 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-17822358

RESUMEN

Mitochondrial ATP synthase (F1Fo-ATPase) is regulated by an intrinsic ATPase inhibitor protein. In the present study, using RT-PCR combined with in silico cloning, we isolated and sequenced the cDNA encoding the inhibitor protein of the giant panda (Ailuropoda melanoleuca). The deduced protein sequence showed that the protein is composed of 106 amino acids and the estimated molecular weight of the ATPIF(1) protein is 12.32 kDa with an isoelectric point (pI) of 10.17. Alignment analysis revealed that the deduced protein sequence shares 66%, 78.3%, 66%, 72.6%, 77.4%, and 78.3% homology with that of Mus musculus, Pan troglodytes, Rattus norvegicus, Bos taurus, Macaca mulatta, and Homo sapiens, respectively. Topology prediction showed that there are three protein kinase C phosphorylation sites, one amidation site, three N-myristoylation sites, one casein kinase II phosphorylation site, and one tyrosine kinase phosphorylation site in the ATPase inhibitor. In particular, amino acids in the region between 39 and 72, which is the minimum sequence showing ATPase inhibitory activity, were highly conserved in the protein.


Asunto(s)
Adenosina Trifosfatasas/antagonistas & inhibidores , Precursores de Proteínas/genética , Ursidae/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Secuencia Conservada , ADN Complementario , Proteínas Mitocondriales , Datos de Secuencia Molecular , Fosforilación , Homología de Secuencia
18.
Int J Biol Sci ; 3(2): 85-90, 2006 Dec 23.
Artículo en Inglés | MEDLINE | ID: mdl-17205108

RESUMEN

We obtained the complete mitochondrial genome of U.thibetanus mupinensis by DNA sequencing based on the PCR fragments of 18 primers we designed. The results indicate that the mtDNA is 16,868 bp in size, encodes 13 protein genes, 22 tRNA genes, and 2 rRNA genes, with an overall H-strand base composition of 31.2% A, 25.4% C, 15.5% G and 27.9% T. The sequence of the control region (CR) located between tRNA-Pro and tRNA-Phe is 1422 bp in size, consists of 8.43% of the whole genome, GC content is 51.9% and has a 6bp tandem repeat and two 10bp tandem repeats identified by using the Tandem Repeats Finder. U. thibetanus mupinensis mitochondrial genome shares high similarity with those of three other Ursidae: U. americanus (91.46%), U. arctos (89.25%) and U. maritimus (87.66%).


Asunto(s)
Genoma Mitocondrial , Ursidae/genética , Animales , ADN Mitocondrial , Genes Mitocondriales , Mitocondrias/genética , Reacción en Cadena de la Polimerasa , Análisis de Secuencia de ADN , Homología de Secuencia
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