[Depression of mPges expression by troglitazone in human renal glomerulus induced by high glucose and LPS].

OBJECTIVE: To determine the influence of troglitanzone on the expression of mPGEs induced by high glucose or LPS in human glomeruli cultured in vitro. METHODS: Human glomeruli were isolated and incubated by LPS or high glucose absence or presence with different concentrations of troglitazone. The concentration of mPGEs in the supernatant was measured by an enzyme-linked immunosorbent assay (ELISA). RESULTS: LPS (10 microg/ml) and 5% glucose could improve the concentration of mPGEs significantly in the supernatant by cultured human glomeruli, and troglitazone (10 - 20 nmol/L) could block its increase (P < 0.05). CONCLUSION: High glucose and LPS can improve mPGEs expression in cultured human glomeruli, and troglitazone can block this effects. These data suggest that PPAR-gamma agonist troglitanzone may be relevant to the treatment of diabetic nephropathy and inflammation in glomerluli.

[Influence of huangqi on TGF-beta 1 inducing extracellular matrix secretion of human peritoneal mesothelial cell].

OBJECTIVE: To study the effect of huangqi on peritoneal sclerosis and its possible mechanism. METHODS: Isolated human peritoneal mesothelial cells (HPMC) was cultured. Cultured HPMC were treated with F12 without serum (control group), F12 with TGF-beta 1 (TGF-beta 1 group), F12 with TGF-beta 1 and huangqi 100 micrograms/ml (huangqi 1 group), F12 with TGF-beta 1 and huangqi 1 mg/ml (huangqi 2 group). Fibronectin(FN) and plasminogen activator inhibitor-1 (PAI-1) were detected by ELISA method. The expression of FN mRNA, PAI-1 mRNA and bFGF mRNA was detected by RT-PCR method. RESULTS: 1. HPMC expressed FN, PAI-1, and bFGF. 2. Fn and PAI-1 significantly increased compared with control with 5 ng/ml TGF-beta 1 stimulated at 24 hours and 48 hours(P < 0.05); 3. The different concentration of huangqi decreased FN and PAI-1 expression compared with TGF-beta 1 group, especially 1 mg/ml huangqi at 24 hours(P < 0.05). 4. FN, PAI-1 and bFGF mRNA expression were higher in 12 hours after stimulation with TGF-beta 1 compared with control. FN, PAI-1 and bFGF mRNA expression were lower in 12 hours after stimulation with different concentration of huangqi (100 micrograms/ml and 1 mg/ml) than those in the TGF-beta 1 group. CONCLUSION: TGF-beta 1 promotes extracellular matrix synthesis and secretion of HPMC. Huangqi partly counteracts the synthesis of human peritoneal mesothelial cell's extracellular matrix by the stimulation of TGF-beta 1.