RESUMEN
It is proposed to use the mixture of acetone and ethylacetate (1:1) as an universal solvent for the extraction of carbofuran from cadaveric tissues and fluids. Extracted carbufuran can be purified from endogenous admixtures on KSS No 3 Silica Gel columns (80/120 mcm) and identified using TLC, electron spectrophotometrty, HPLC, and GC-MS. The proposed method of forensic chemical analysis of carbofuran was applied for the purpose of forensic medical expertise.
Asunto(s)
Carbofurano , Acetatos , Acetona , Carbofurano/análisis , Carbofurano/química , Carbofurano/envenenamiento , Etanol , Toxicología Forense/métodos , Humanos , Insecticidas/análisis , Insecticidas/química , Insecticidas/envenenamiento , Hígado/patología , Espectrometría de Masas/métodos , SolventesRESUMEN
The objective of the present work was to study the distribution of tetramethylthiuram disulfide (TMTD) in the body of warm-blooded animals as exemplified by white rats. TMTD was administered intragastrically and detected in the unaltered form in other internal organs. It was found to accumulate in the largest amounts in the gastric contents, kidneys, and urine.
Asunto(s)
Tiram/farmacocinética , Administración Oral , Animales , Cromatografía Líquida de Alta Presión , Cromatografía en Capa Delgada , Contenido Digestivo/química , Riñón/metabolismo , Masculino , Ratas , Espectrofotometría Ultravioleta , Tiram/orina , Distribución TisularRESUMEN
Optimal conditions for the extraction of tetramethyltiuram disulfide (TMTD) from biological materials with ethylacetate are described. The isolated compound can be separated from co-extracted impurities on a silicagel L (40/100 mcm) column. Identification and quantitative determination of TMTD purified from cadaverous liver were performed using thin-layer chromatography, IR spectrophotometry, and high performance liquid chromatography with UF detection.
Asunto(s)
Acetatos/química , Medicina Legal/métodos , Fungicidas Industriales/análisis , Hígado/química , Tiram/análisis , Adulto , Cadáver , Cromatografía Líquida de Alta Presión/métodos , Cromatografía en Capa Delgada/métodos , Humanos , Masculino , Sensibilidad y Especificidad , Espectrofotometría Infrarroja/métodosRESUMEN
Optimal conditions for the identification and quantitative determination of tetraethyltiuram disulfide in "fresh" and putrefactive tissues of cadaverous liver are described for the purpose of TLC, HPLC, and IK-spectrophotometry following extraction of the compound of interest with ethyl acetate and its purification on a silicagel L column, 40/100 mcm. The persistence of tetraethyltiuram disulfide in the cadaverous tissues was evaluated. It was shown that the period during which tetraethyltiuram disulfide can be detected in the autopsied tissues decreases from 203 to 28 days with a rise in temperature from -15 degrees C to +36 degrees C.
Asunto(s)
Disulfiram/análisis , Ciencias Forenses/métodos , Hígado/química , Cromatografía Líquida de Alta Presión/métodos , Cromatografía en Capa Delgada/métodos , Disulfiram/química , Humanos , Sensibilidad y Especificidad , Espectrofotometría Infrarroja/métodosRESUMEN
Optimal conditions for the extraction of bancol from the biological material with toluene are described. The possibility of its purification and separation from co-extracted compounds on a silicagel L column, 40/100 mcm is illustrated. Identification and quantitative determination of bancol isolated from the cadaverous liver were performed by the electron spectrophotometry technique, thin-layer chromatography, and high performance liquid chromatography using normal-phase sorbents. A method of bancol detection was adapted for the purpose of forensic medical expertise and applied for the postmortem examination of the cadaverous tissues.
Asunto(s)
Bencenosulfonatos/análisis , Toxicología Forense/métodos , Hígado/química , Espectroscopía de Fotoelectrones/métodos , Bencenosulfonatos/química , Cromatografía Líquida de Alta Presión/métodos , Cromatografía en Capa Delgada/métodos , Humanos , Tolueno/químicaRESUMEN
Optimal conditions have been determined for furadan isolation from biological fluids by means of the mixture of solvents ethyl acetate-acetone in volume 1:1. Possible purification of the compound from coextractive substances of biomaterial on the column with silica gel L 100/160 mcm is demonstrated. IR-spectrophotometric methods and high performace liquid chromatography with a detector of photodiode matrix are proposed for identification and quantitation of furadan in blood and urine specimens.
